BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0512.p (137 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogena... 271 2e-74 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 80 6e-17 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 74 5e-15 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 70 6e-14 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 69 2e-13 >gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 271 bits (692), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 130/133 (97%), Positives = 131/133 (98%) Query: 5 PVRRATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIPAE 64 P +ATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIPAE Sbjct: 126 PHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIPAE 185 Query: 65 VMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTTGQWIF 124 VMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTTGQWIF Sbjct: 186 VMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTTGQWIF 245 Query: 125 VDGGYTHLDRALT 137 VDGGYTHLDRALT Sbjct: 246 VDGGYTHLDRALT 258 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 80.1 bits (196), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 50/127 (39%), Positives = 69/127 (54%), Gaps = 5/127 (3%) Query: 3 ARPVRRATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIP 62 AR + R +RG I+N +S + G G GY ASKG LT+ A A DG+RVNA+ P Sbjct: 117 ARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAP 176 Query: 63 AEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTTGQW 122 + TPL + A D P + I ++ PLG R+ E++ + FL S +S TG Sbjct: 177 GWIATPLTQ---ALQDDPV-RAGPILARTPLG-RWGQPEDVGQVVAFLCSPAASFMTGAI 231 Query: 123 IFVDGGY 129 + VDGGY Sbjct: 232 VPVDGGY 238 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 73.9 bits (180), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 43/120 (35%), Positives = 62/120 (51%), Gaps = 2/120 (1%) Query: 13 AIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIPAEVMTPLYEK 72 ++I SS T+V G N S Y ASK A + R WA L+D +RVN + P + TP + Sbjct: 132 SVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNVVSPGPIRTPGLGE 191 Query: 73 WIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTTGQWIFVDGGYTHL 132 + D Q D + S++PLG R +E+ FL S+ +S G +FVDGG + Sbjct: 192 LVPD-DARQGLFDYLASQVPLG-RLGEPQEIGKAVAFLASDAASFVNGIELFVDGGMAQV 249 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 70.1 bits (170), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 53/127 (41%), Positives = 70/127 (55%), Gaps = 9/127 (7%) Query: 5 PVRRATRGA-IINVSSKTAVTGQGNTS-GYCASKGAQLSLTREWAAALRDDGVRVNALIP 62 PV RA G IINV+S A G G + Y ASKG +LTR A L D +RVNA+ P Sbjct: 129 PVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRVNAVAP 188 Query: 63 AEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSER-SSHTTGQ 121 +MTP E+ F P E+L+ IP+G R +E + ++L SE+ S + TGQ Sbjct: 189 GVIMTPFQER----FTTP-EQLEGFRKTIPMG-RIGEPDECSGAFLYLASEQMSGYVTGQ 242 Query: 122 WIFVDGG 128 I V+GG Sbjct: 243 IIDVNGG 249 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 68.9 bits (167), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 40/122 (32%), Positives = 67/122 (54%), Gaps = 2/122 (1%) Query: 7 RRATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIPAEVM 66 R+ GA IN++S + + + Y SKGA ++ ++ AA L D +RVN + P Sbjct: 130 RQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRVNCVNPVISA 189 Query: 67 TPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTTGQWIFVD 126 T L +++ D P+ + + IP+G RF+T E++A+ ++L S+ + TG I VD Sbjct: 190 TGLLSEFMGVPDTPENR-KKFVATIPMG-RFSTPEDIANACLYLGSDEAEFVTGVCIEVD 247 Query: 127 GG 128 GG Sbjct: 248 GG 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 434,986 Number of extensions: 15884 Number of successful extensions: 115 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 20 Length of query: 137 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 61 Effective length of database: 1,260,010 Effective search space: 76860610 Effective search space used: 76860610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)