BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0500.p (219 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein... 442 e-125 gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogena... 82 5e-17 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 62 4e-11 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 60 2e-10 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 59 4e-10 >gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 283 Score = 442 bits (1137), Expect = e-125, Method: Compositional matrix adjust. Identities = 218/219 (99%), Positives = 218/219 (99%) Query: 1 VALSAPRAEVLEEVAGAHPEAFIAPLDVLCHDAWRDQHARIVEAFGGVDLLIFCAARYRP 60 VALSA RAEVLEEVAGAHPEAFIAPLDVLCHDAWRDQHARIVEAFGGVDLLIFCAARYRP Sbjct: 65 VALSARRAEVLEEVAGAHPEAFIAPLDVLCHDAWRDQHARIVEAFGGVDLLIFCAARYRP 124 Query: 61 ERSWEVQEDEAEHTIRTNLASVYSALGAALPDMLARGSGGIALVASVAGYVGLPGATVYG 120 ERSWEVQEDEAEHTIRTNLASVYSALGAALPDMLARGSGGIALVASVAGYVGLPGATVYG Sbjct: 125 ERSWEVQEDEAEHTIRTNLASVYSALGAALPDMLARGSGGIALVASVAGYVGLPGATVYG 184 Query: 121 PGKAALINLAEILYSDLRPKGVNVYLVNPGFVKTDLTDKNDFTMPALQTPQQAASAIWRG 180 PGKAALINLAEILYSDLRPKGVNVYLVNPGFVKTDLTDKNDFTMPALQTPQQAASAIWRG Sbjct: 185 PGKAALINLAEILYSDLRPKGVNVYLVNPGFVKTDLTDKNDFTMPALQTPQQAASAIWRG 244 Query: 181 IGEGRFEIHFPLRFTAWLKLLRILPFRLRFMLFQRFLLS 219 IGEGRFEIHFPLRFTAWLKLLRILPFRLRFMLFQRFLLS Sbjct: 245 IGEGRFEIHFPLRFTAWLKLLRILPFRLRFMLFQRFLLS 283 >gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 268 Score = 81.6 bits (200), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 71/225 (31%), Positives = 104/225 (46%), Gaps = 11/225 (4%) Query: 1 VALSAPRAEVLEEVAGAHPEA---FIAPLDVLCHDAWRDQHARIVEAFGGVDLLIFCAAR 57 + L A R LE + A P A + LDV H A ++ FGG D++I A Sbjct: 37 LGLVARRTAELEALRAALPHAERHRVYALDVTDHAALAAAATSFMDEFGGADVVIANAGI 96 Query: 58 YRPERSWEVQEDEA--EHTIRTNLASVYSALGAALPDMLA-----RGSGGIALVASVAGY 110 + + E QED A E + N+ + ++ + M A R + + SVAG Sbjct: 97 SQGTLT-EFQEDLAAFERILAVNVTATFATFTPFVARMKASPEAQRRRCRLVGIGSVAGI 155 Query: 111 VGLPGATVYGPGKAALINLAEILYSDLRPKGVNVYLVNPGFVKTDLTDKNDFTMPALQTP 170 GLPGA Y K+A+I+ E L +LR G+ V + PG++ T +T N + MP L Sbjct: 156 RGLPGAGAYSASKSAVISYCESLRVELRASGIKVVTIAPGYIDTPMTRVNTYRMPFLMPV 215 Query: 171 QQAASAIWRGIGEGRFEIHFPLRFTAWLKLLRILPFRLRFMLFQR 215 ++ A+ + I EG P + KLLR+LP L M F R Sbjct: 216 ERFAARAVQTIDEGVSYRVIPWQMGVVAKLLRMLPNWLYDMAFAR 260 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 61.6 bits (148), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 49/160 (30%), Positives = 74/160 (46%), Gaps = 6/160 (3%) Query: 3 LSAPRAEVLEEVAG----AHPEAFIAPLDVLCHDAWRDQHARIVEAFGGVDLLIFCAARY 58 L+A R + LE+VA A + DV A ++ FGG+D+ I A Sbjct: 35 LTARRQDALEQVAALICAQGGRASVVAGDVGEAATHERGVAMALDEFGGLDIAINNAGAV 94 Query: 59 RPERSW-EVQEDEAEHTIRTNLASVYSALGAALPDMLARGSGGIALVASVAGY-VGLPGA 116 + E+ + + T+ NL S + +P MLARG+G I +S G GLPG Sbjct: 95 GACKPLAEISPQQWQDTLHANLTSAFLGARCQIPAMLARGAGAIVFTSSFVGSSAGLPGM 154 Query: 117 TVYGPGKAALINLAEILYSDLRPKGVNVYLVNPGFVKTDL 156 YG KA L+ L + + +D +G+ + PG V TD+ Sbjct: 155 AAYGAAKAGLMGLVKGIAADYAVQGIRANALLPGGVDTDM 194 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 38/126 (30%), Positives = 61/126 (48%), Gaps = 3/126 (2%) Query: 34 WRDQHA---RIVEAFGGVDLLIFCAARYRPERSWEVQEDEAEHTIRTNLASVYSALGAAL 90 W A ++ G VD+L+ A R + ++ +D+ + I TNL S+++ + Sbjct: 65 WESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQVI 124 Query: 91 PDMLARGSGGIALVASVAGYVGLPGATVYGPGKAALINLAEILYSDLRPKGVNVYLVNPG 150 M RG G I ++SV G G G T Y KA + L ++ KGV V V+PG Sbjct: 125 EGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPG 184 Query: 151 FVKTDL 156 ++ TD+ Sbjct: 185 YIGTDM 190 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 58.5 bits (140), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 46/150 (30%), Positives = 68/150 (45%), Gaps = 5/150 (3%) Query: 10 VLEEVAGAHPEAFIAPLDVLCHDAWRDQHARIVEAFGGVDLLIFCAA---RYRPERSWEV 66 V+EE+ A A A DV A +E FG +D+++ A R RP EV Sbjct: 45 VVEEIKAAGGRAVFAKADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRP--MLEV 102 Query: 67 QEDEAEHTIRTNLASVYSALGAALPDMLARGSGGIALVASVAGYVGLPGATVYGPGKAAL 126 +EDE + N+ S++ + +P G G +AS AG PG T Y K A+ Sbjct: 103 EEDEFDRVYAVNVKSIFLSAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAV 162 Query: 127 INLAEILYSDLRPKGVNVYLVNPGFVKTDL 156 I ++ + ++L P + V VNP T L Sbjct: 163 ITTSKSMAAELGPDKIRVNCVNPVISATGL 192 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 739,482 Number of extensions: 32259 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 225 Number of HSP's successfully gapped: 16 Length of query: 219 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 137 Effective length of database: 1,231,600 Effective search space: 168729200 Effective search space used: 168729200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)