BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0492.p (548 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 1085 0.0 gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 92 1e-19 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 79 1e-15 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 78 2e-15 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 67 3e-12 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 1085 bits (2805), Expect = 0.0, Method: Compositional matrix adjust. Identities = 542/548 (98%), Positives = 544/548 (99%) Query: 1 METYLIGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAV 60 METYLIGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAV Sbjct: 1 METYLIGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAV 60 Query: 61 RAVVAKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRIN 120 RA V KSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRIN Sbjct: 61 RAAVTKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRIN 120 Query: 121 RMGHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQRSSC 180 RMGHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQRSSC Sbjct: 121 RMGHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQRSSC 180 Query: 181 TVTCKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMG 240 TVTCKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMG Sbjct: 181 TVTCKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMG 240 Query: 241 LRPGIAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWG 300 LRPGIAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWG Sbjct: 241 LRPGIAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWG 300 Query: 301 PYYNAMAPGMWLNEGGQSAAGAAIDHLLRLHPATPEAREEAARAQMELPQWLAQQALAAV 360 PYY AMAPGMWLNEGGQSAAGAAIDHLLRLHPATP+AREEAARAQMELPQWLAQQALAAV Sbjct: 301 PYYKAMAPGMWLNEGGQSAAGAAIDHLLRLHPATPQAREEAARAQMELPQWLAQQALAAV 360 Query: 361 VHPSEAVWLAGQLTVVPEFLGNRAPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGY 420 HPSEAVWLAGQLTVVPEFLGNR+PLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGY Sbjct: 361 DHPSEAVWLAGQLTVVPEFLGNRSPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGY 420 Query: 421 GLRQIIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEPVLLGSAMLA 480 GLRQIIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEPVLLGSAMLA Sbjct: 421 GLRQIIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEPVLLGSAMLA 480 Query: 481 AVAAGLHADLQSAMPAMSSVAGRSLPAQGDVAALHAARYQAFLKSQQLARELRDSLAPLL 540 AVAAGLHADLQSAMPAMSSVAGRSLPAQGDVAALHAARYQAFLKSQQLARELRDSLAPLL Sbjct: 481 AVAAGLHADLQSAMPAMSSVAGRSLPAQGDVAALHAARYQAFLKSQQLARELRDSLAPLL 540 Query: 541 AAHTQGHH 548 AAHTQGHH Sbjct: 541 AAHTQGHH 548 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 92.0 bits (227), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 129/507 (25%), Positives = 198/507 (39%), Gaps = 75/507 (14%) Query: 4 YLIGIDVGTGSARAGVFDPQGQLLASARHDI-DLFRDERRARVEQSSAQIWEAVCQAVRA 62 YL+ +D GT S+R+ +FD G ++A+A+ + +F + VE +W + Q + Sbjct: 3 YLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIF--PQPGWVEHDPMDLWHSQLQTAQK 60 Query: 63 VVAKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRA--LEQAQRIN 120 V+A+SGI + + +G+ V A G P + +VW D RA + A R Sbjct: 61 VLAQSGIKASELTALGITNQRETTVVWERA----TGKPVYNAVVWQDRRAEVVCAALRER 116 Query: 121 RMGHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAH----FFDLTDFLTWKATGSLQ 176 +G A+ G V+ P KL W+ +++ + A A F + +L W TG Sbjct: 117 GLGEAIQKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRL 176 Query: 177 RSSCTVTCKWTYL--AHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQT 234 S T L H WD + W G P Sbjct: 177 HVSDVSNASRTMLWNIHTGAWDEELLQ--------------------WLGIP-------- 208 Query: 235 AAQAMGLRPGIAVAAGLIDA-HAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPV 293 AA ++P A G IDA H G + G +A +FG +C A Sbjct: 209 AAMLPQVQPS-AHLYGEIDAQHLGAAVKIGGIAGDQQSA-----LFG-QACVRPGMAKNT 261 Query: 294 LVPGVWGPYYNAMAPGMWLNEGGQSAA---GAAIDHLLRLHPATPEAREEAARAQMELPQ 350 G + M L+ G Q A G ++ A E + + Q Sbjct: 262 YGTGCF----------MLLHTGDQCATSHNGLISTAACQVDEQKQYALEGSVFIGGAVVQ 311 Query: 351 WLAQQALAAVVHPSEAVWLA------GQLTVVPEFLGNRAPLADPQARAALVGLGMEHDI 404 WL + L A+ ++ LA G + VP F G AP +P A+ A+VGL + Sbjct: 312 WL-RDGLKAIKTSTDVEGLACSVEDSGGVVFVPSFTGLGAPYWNPSAKGAIVGLSRGSTV 370 Query: 405 DSLVALYVAGLCSLGYGLRQIIEAQAACGVR-VQSISVSGGAGVHPLTRQLLADATGMPV 463 + A L S+ + ++ A V + + V GGA + L Q AD G+PV Sbjct: 371 AHIAR---AALESIAFQSTALLNAMVRDAVSPITELRVDGGAAANNLLLQFQADLLGIPV 427 Query: 464 EITASPEPVLLGSAMLAAVAAGLHADL 490 E LG+A LA +A G++ DL Sbjct: 428 IRPQVTETTALGAAYLAGIATGVYRDL 454 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 79.0 bits (193), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 122/502 (24%), Positives = 191/502 (38%), Gaps = 44/502 (8%) Query: 4 YLIGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAVRAV 63 Y++G+D+GT S ++ VF G+++A + + E EQ QI A ++ Sbjct: 9 YMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEP-GMAEQDPEQIVAAALASIAGA 67 Query: 64 VAKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRINRM- 122 V + P +A + A V+ + + I W D RA A+RI Sbjct: 68 VKAAKAAPQEIALLSFSAAMHSVI-----ALDAENRLLSNSITWGDIRASVWAERIKHEH 122 Query: 123 -GHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQRSSCT 181 G+A+ G + P KL+W++ P+V+ AA F + ++L ++ G Sbjct: 123 DGNAIYRRTGTPVHPMSPLCKLMWMRHEKPDVFHRAARFVGIKEYLLYQLFGQWVVDHSI 182 Query: 182 VTCKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMGL 241 + + E WD G LA G P T L+ AQ +GL Sbjct: 183 ASATGMFNLRELAWDQ--------GALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGL 234 Query: 242 RPGIAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVP-GVWG 300 G D +G A G +A GTS T +P P G Sbjct: 235 SVDTPFIIGANDGVLSNLGVNAIEIGH------VAVTIGTSGAMRTVIDEPRTDPQGRLF 288 Query: 301 PYYNAMAPGMWLNEGGQSAAGAAIDHLLRLHPATPEAREEAARAQMELPQWLAQQALAAV 360 Y A+ W+ GG G I +R E AA + + + A +A Sbjct: 289 CY--ALTEKHWV-VGGPVNNGGNIFRWVR---DELATAEAAAAREEGVDPYEALTRIAEK 342 Query: 361 VHPSEAVWLAGQLTVVPEFLGNRAPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGY 420 V P A L P G RAPL + R + GL + H ++ + G+ + Sbjct: 343 VRPG-----AEGLLFHPFMAGERAPLWNADLRGSFFGLALHHGKHHMIRAALEGVI---F 394 Query: 421 GLRQIIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEPVLLGSAMLA 480 L I+ A + + +GG L RQ++AD V + S E LG+A+L Sbjct: 395 NLYSILPALEELVGPTKRMMATGGFARSALWRQMMADIFNREVVVPESVESSCLGAAVLG 454 Query: 481 AVAAGLHADLQSAMPAMSSVAG 502 A A GL +P++S ++G Sbjct: 455 AWALGL-------VPSLSVISG 469 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 78.2 bits (191), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 139/544 (25%), Positives = 205/544 (37%), Gaps = 58/544 (10%) Query: 6 IGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAVRAVVA 65 +GID+GT +A V D QG L+A A +++ R R EQ+ A W+A C V + Sbjct: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWS-EQAPADWWQATCDTVAKLRT 61 Query: 66 KSGIDP-AAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRINRMGH 124 + G + ++ IG+ + GA + + R I+W D R+ + + Sbjct: 62 QLGSERFGSIRAIGLSGQ----MHGAVL-LDERDEVLRPAILWSDSRSAPECAELESRAP 116 Query: 125 AVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQRSSCTVTC 184 + G + P PKLLW+ + P+++A A D+L K TG Sbjct: 117 RLHGIAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAG 176 Query: 185 KWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMGLRPG 244 W LA +G R + G+ ++G L AQA GLR Sbjct: 177 TLWLDVEGRNWSDEL--------LAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSD 228 Query: 245 IAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWGPYYN 304 + VA G D A VG A + G G + GTS + PG + Sbjct: 229 VIVAGGAGDGAASAVGIGAVKPGDGFLS------LGTSGVLFVVNDRFRPNPGRAIHAFC 282 Query: 305 AMAPGMWLNEGGQSAAGAAIDHLLRLHPATPEAREEAARAQMELPQWLAQQALAAVVHPS 364 P W +A + + RL E++ A++E L +QA Sbjct: 283 HTLPQRWHQMSVMLSAASCLRWFCRLC----SVDEKSLLAEIEQ---LDEQACNNAP--- 332 Query: 365 EAVWLAGQLTVVPEFLGNRAPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGYGLRQ 424 +P G R P DP A GL EH AL A L + +G+ Sbjct: 333 ---------LFLPYLSGERTPHNDPYATGVFHGLTPEH---QRAALGYAVLEGVAFGMVD 380 Query: 425 IIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEP-VLLGSAMLAAVA 483 ++A A G V +S+ GG QLLADA + + E LG+A LA +A Sbjct: 381 GLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIVTHVGSEAGGALGAARLAWLA 440 Query: 484 AGLHADLQSAMPAMSSVAGRSLPAQGDVAALHAARYQAFLKSQQLARELRDSLAPLLAAH 543 G + V + PAQ + AR+ A Q R + APL H Sbjct: 441 DGGDE---------AEVCRK--PAQQALYQPDPARHAALQARLQRYRAIYAPFAPL---H 486 Query: 544 TQGH 547 +GH Sbjct: 487 GEGH 490 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 67.4 bits (163), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 125/488 (25%), Positives = 186/488 (38%), Gaps = 61/488 (12%) Query: 6 IGIDVGTGSARAGVFDPQGQLLAS--ARHDIDLFRDERRARVEQSSAQIWEAVCQAVRAV 63 +GID+GT + + D ++AS AR +D + EQ+ W A A+ A+ Sbjct: 4 LGIDLGTSEVKLVLTDEDSNVIASTSARLRVD---NPHPLWSEQAPQSWWNATLDAIAAL 60 Query: 64 VAKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRINRMG 123 ++ A+ GIG+ + GA+ + G+ R I+W D RA + + + Sbjct: 61 RTQAPQAFQALRGIGLSGQ----MHGATL-LDRNGNVLRPAILWNDMRAHAECVELEALV 115 Query: 124 HAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSL-QRSSCTV 182 ++ G P PKLLWL + P VY + DFL WK TG S Sbjct: 116 PDAVAITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDFLGWKLTGEFVSEMSDAA 175 Query: 183 TCKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMGLR 242 W +A W R +G L + R+ G+ +AG L + G+ Sbjct: 176 GTLWLDVARR-DWSE---RMLGATGLDRSHMPRLV-----EGSAVAGQLRDELRREWGIS 226 Query: 243 PGIAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTM--TSHADPVLVPGVWG 300 + VA G D A VG R GDA + G+S T+H P GV Sbjct: 227 GPVVVAGGAGDNAASAVGIGVIRA--GDAFLSL----GSSGVLFAATAHHAPNAQQGVHA 280 Query: 301 PYYNAMAPGMWLNEGGQSAAGAAIDHLLRLHPATPEAREEAARAQMELPQWLAQQALAAV 360 + PG W +A A++ H L A P +A + Sbjct: 281 --FCHCLPGQWHQMSVILSAAASL-HWLSGVTARP---------------------VAEL 316 Query: 361 VHPSEAVWLAGQLTVVPEFL----GNRAPLADPQARAALVGLGMEHDIDSLVALYVAGLC 416 V +E + AGQ P FL G R P D A+ L G+ H+ L + G+ Sbjct: 317 VSGAERL-SAGQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMTPAHESAHLAYAVMEGVA 375 Query: 417 SLGYGLRQIIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEP-VLLG 475 + + A A G ++S + GG L A A G+P+ A E LG Sbjct: 376 ---FAMADGYAALQAAGTTLESAAFVGGGSRSAFWGVLCATALGIPLRRHAGAEVGAALG 432 Query: 476 SAMLAAVA 483 +A L +A Sbjct: 433 AARLGRLA 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,778,735 Number of extensions: 73906 Number of successful extensions: 323 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 312 Number of HSP's successfully gapped: 6 Length of query: 548 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 458 Effective length of database: 1,193,720 Effective search space: 546723760 Effective search space used: 546723760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)