BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0490.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 350 3e-98 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 112 2e-26 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 98 3e-22 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 94 5e-21 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 89 3e-19 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 350 bits (899), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 173/174 (99%), Positives = 174/174 (100%) Query: 1 MAEAGTADFLVNSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGA 60 MAEAGTADFL+NSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGA Sbjct: 68 MAEAGTADFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGA 127 Query: 61 MVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAA 120 MVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAA Sbjct: 128 MVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAA 187 Query: 121 WSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFLAV 174 WSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFLAV Sbjct: 188 WSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFLAV 241 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 112 bits (279), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 69/165 (41%), Positives = 94/165 (56%), Gaps = 4/165 (2%) Query: 8 DFLVNSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGAMVNITSI 67 D +VN AGI ++ E+ E +E V+ VNL + C A AR + Q G +VN S+ Sbjct: 79 DIVVNCAGI--IKRGAELETEVFEQVIAVNLTGTMRVCAA-ARELLRQSRG-CIVNTASM 134 Query: 68 AGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAAWSDPAKS 127 G Y+ASK G+ T+ LA GIRVNAVAP T L +A DP ++ Sbjct: 135 LSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQDDPVRA 194 Query: 128 GPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFL 172 GP++AR P R+ PEDV + +A L SP A+ + GA+VPVDGG+L Sbjct: 195 GPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGYL 239 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 98.2 bits (243), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 59/167 (35%), Positives = 93/167 (55%), Gaps = 3/167 (1%) Query: 5 GTADFLVNSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGAMVNI 64 G+ LVN+AGI + + M DE +++V+ NL A +A R + + G ++NI Sbjct: 85 GSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAVLR-GMMKARTGRIINI 143 Query: 65 TSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAAWSDP 124 TS+ G G + YAA+KAG+ G +R LARE+G I VN VAP T++ A + Sbjct: 144 TSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAPGFIDTDMTRAL--NE 201 Query: 125 AKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGF 171 +S ++ + P R + E++A ++A L SP AA + G + V+GG Sbjct: 202 QQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNGGM 248 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 94.4 bits (233), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 59/171 (34%), Positives = 93/171 (54%), Gaps = 2/171 (1%) Query: 3 EAGTADFLVNSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGAMV 62 + G D LVN+AG+ S L+ E + + VN+ L+ Q AR+ + Q G +V Sbjct: 82 DYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAARLMVKQQWG-RIV 140 Query: 63 NITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAAWS 122 NI+SI+G R Y SKA + G TR +A EL +GI VN+VAP T + +A S Sbjct: 141 NISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPVDTPMTQAMHS 200 Query: 123 DPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFLA 173 + + P R+ + +++A ++ L + ++A + G V+PVDGGF+A Sbjct: 201 EQTRE-SYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGGFVA 250 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 88.6 bits (218), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 57/170 (33%), Positives = 85/170 (50%), Gaps = 5/170 (2%) Query: 5 GTADFLVNSAGINVLES-VLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGAMVN 63 G D +VN+AG +LE+ ++ ++ V VN+++ ++ + F Q GGGA +N Sbjct: 81 GKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFLSAKTFVPY-FRQVGGGAFIN 139 Query: 64 ITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAAW-- 121 I S AG R Y SK + ++ +A ELGP IRVN V P I+ T L Sbjct: 140 IASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRVNCVNPVISATGLLSEFMGV 199 Query: 122 -SDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGG 170 P +A P RF++PED+A + L S +A + G + VDGG Sbjct: 200 PDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVTGVCIEVDGG 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 567,554 Number of extensions: 22968 Number of successful extensions: 193 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 40 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)