BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0485.p (422 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 800 0.0 gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 144 1e-35 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 110 2e-25 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 95 1e-20 gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 56 5e-09 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 800 bits (2066), Expect = 0.0, Method: Compositional matrix adjust. Identities = 419/422 (99%), Positives = 420/422 (99%) Query: 1 MSTPGIIEAPLTETAAPATAPAPTAHKAGQEVPANWSGIASLALGVFSLVTAEFLPASLL 60 MSTPGIIEAPLTETAAPATAPAPTAHKAGQE PANWSGIASLALGVFSLVTAEFLPASLL Sbjct: 1 MSTPGIIEAPLTETAAPATAPAPTAHKAGQEAPANWSGIASLALGVFSLVTAEFLPASLL 60 Query: 61 TAIATDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNIDRKRVVLMLSLLLLVSNILAV 120 TAIA+DLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNIDRKRVVLMLSLLLLVSNILAV Sbjct: 61 TAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNIDRKRVVLMLSLLLLVSNILAV 120 Query: 121 TAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFARAMSLILTGVSVATVCAAPI 180 TAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFARAMSLILTGVSVATVCAAPI Sbjct: 121 TAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFARAMSLILTGVSVATVCAAPI 180 Query: 181 GAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDHPSLKVLAELLGRKDVRVALL 240 GAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDHPSLKVLAELLGRKDVRVALL Sbjct: 181 GAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDHPSLKVLAELLGRKDVRVALL 240 Query: 241 AVLLVVSGHFAGFTYIRPLMEQITHMSVATISAVLLGYGVGGFFGNFAGGFIAERSERYA 300 AVLLVVSGHFAGFTYIRPLMEQITHMSV TISAVLLGYGVGGFFGNFAGGFIAERSERYA Sbjct: 241 AVLLVVSGHFAGFTYIRPLMEQITHMSVTTISAVLLGYGVGGFFGNFAGGFIAERSERYA 300 Query: 301 IVSGGALIALLAASLWVAGSSPVVAAVAITLWGFAFGAFPVGFQTWIVRAAPDHAEGAGG 360 IVSGGALIALLAASLWVAGSSPVVAAVAITLWGFAFGAFPVGFQTWIVRAAPDHAEGAGG Sbjct: 301 IVSGGALIALLAASLWVAGSSPVVAAVAITLWGFAFGAFPVGFQTWIVRAAPDHAEGAGG 360 Query: 361 LLVAAFQVAIASGAIGGGVLVDHVGALGGPIFAVVAITLGTLLTLRYGPRGGGAPVAGNA 420 LLVAAFQVAIASGAIGGGVLVDHVGALGGPIFAVVAITLGTLLTLRYGPRGGGAPVAGNA Sbjct: 361 LLVAAFQVAIASGAIGGGVLVDHVGALGGPIFAVVAITLGTLLTLRYGPRGGGAPVAGNA 420 Query: 421 HF 422 HF Sbjct: 421 HF 422 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 144 bits (364), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 105/343 (30%), Positives = 176/343 (51%) Query: 41 SLALGVFSLVTAEFLPASLLTAIATDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNID 100 +L + F++ T EF+ LL +A +L VS +AG VT A+ V AP + T + Sbjct: 7 ALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGRLP 66 Query: 101 RKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFA 160 RK +++ L ++ +V N+ AP+ L++ RV+ A G F+ + + +A LVP A Sbjct: 67 RKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQA 126 Query: 161 RAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDH 220 A++L+ TG++VA V P G ++G +GWR+ F A V+ ++A+A + +PR + Sbjct: 127 SAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGGEE 186 Query: 221 PSLKVLAELLGRKDVRVALLAVLLVVSGHFAGFTYIRPLMEQITHMSVATISAVLLGYGV 280 +L R V + LL +L G FA FTYI P++ ++ + +S +LL +G+ Sbjct: 187 TDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLLFGL 246 Query: 281 GGFFGNFAGGFIAERSERYAIVSGGALIALLAASLWVAGSSPVVAAVAITLWGFAFGAFP 340 G GN AGG +A++ ++ L+A + + + V A + + L G A A Sbjct: 247 GLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGMAAFATV 306 Query: 341 VGFQTWIVRAAPDHAEGAGGLLVAAFQVAIASGAIGGGVLVDH 383 Q ++ A A +AAF + A+GA GGV +DH Sbjct: 307 PPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDH 349 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 104/351 (29%), Positives = 164/351 (46%), Gaps = 7/351 (1%) Query: 35 NWSGIASLALGVFSLVTAEFLPASLLTAIATDLHVSDGAAGQAVTATAIVGAVAAPSIPL 94 NW + +LA+ F + T EF+ LL +A DL V+ AAG V+ A+ A AP + + Sbjct: 2 NWP-LFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAI 60 Query: 95 LTRNIDRKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLV 154 LT RK+ ++ L L ++ N+L APN L+VARV+ F+ + + +A LV Sbjct: 61 LTAKWPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELV 120 Query: 155 PDHLFARAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPR 214 A+A++L+ TG++VA + P+G G+ GWRS F A + ++A + W +P+ Sbjct: 121 APQKRAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPK 180 Query: 215 LPPKDHPSLKVLAELLGRKDVRV--ALLAVLLVVSGHFAGFTYIRPLMEQITHMSVATIS 272 P + + E D RV +LL L F+ TYI ++ +T + S Sbjct: 181 KIPMQAGN--IFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQES 238 Query: 273 AVLLGYGVGGFFGNFAGGFIAERSERYAIVSGGALIALLAASLWVAGSSPVVAAVAITLW 332 VLL +G G G GG +A+ + IA++ + + W Sbjct: 239 WVLLLFGAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSHYKWPTLATMFAW 298 Query: 333 G-FAFGAFPVGFQTWIVRAAPDHAEGAGGLLVAAFQVAIASGAIGGGVLVD 382 G FAF P G Q ++ A A L AF + A+GA GG+ + Sbjct: 299 GAFAFAVVP-GAQIRVLDTARGAPNLASTLNQGAFNLGNATGAWVGGLAIS 348 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 94.7 bits (234), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 111/391 (28%), Positives = 186/391 (47%), Gaps = 31/391 (7%) Query: 7 IEAPLTETAAPATAPAPTAHKAGQEVPANWSGIASLALGVFSLVTAEFLPASLLTAIATD 66 + P +AP+ +PT +AG + A + LALG F++ T F+ A LL +A D Sbjct: 1 MNPPPLGPSAPSACASPT--QAGSLLTAGF-----LALGTFAIGTEGFMIAPLLPRMAAD 53 Query: 67 LHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNIDRKRVVLMLSLLLLVSNILAVTAPNLP 126 +S V +V A+++P +L+ ++R+RV++ +N+LA + + Sbjct: 54 FGMSVPMVASLVVIFTLVLALSSPISTVLSAAMNRRRVLIAAMTAFTAANLLAACSSSFT 113 Query: 127 VLLVARVLLGVALGGFWSMAAALAMRLVPDHLFARAMSLILTGVSVATVCAAPIGAYMGN 186 LLVAR+L+ VA G + A+ALA +V RA++++ G++ + P+GA +G+ Sbjct: 114 GLLVARLLMAVAAGLYTPNASALAGAMVAPGQRGRALAIVSGGMTSPSALGLPLGAVVGH 173 Query: 187 LWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDHPSLKVLAE---LLGRKDVRVALLAVL 243 +GWR+ F+A + VA+A + +PR H + L E ++G+ V+ L L Sbjct: 174 AFGWRATFLAVSAMGAVALAGILLGVPR-QAGAHLHVAGLRERMAVIGQPQVQRLLAVTL 232 Query: 244 LVVSGHFAGFTYIRPLMEQITHMSVATISAVLLGYGVGGFFGNFAGGFIAER--SERYAI 301 L G +A + YI P + + + IS + +G G GG + +R S R Sbjct: 233 LWSVGAYAAYPYIAPYLSHVLSFGESRISLTVSLWGAAAAVGVSVGGTLNDRYGSARVVR 292 Query: 302 VSGGALIALLAASLWVAGSS----------PVVAAVAITLWGFAFGA-FPVGFQTWIVRA 350 VS + LL S W ++ PV+ +A+ +WGF+ A FP I Sbjct: 293 VS----LLLLMLSFWTLAAATALPPPLALGPVL--LAVVVWGFSVWAFFPAQMARLIGAG 346 Query: 351 APDHAEGAGGLLVAAFQVAIASG-AIGGGVL 380 AP A A L + + + G A G G+L Sbjct: 347 APAQAPVALSLNTSTLYLGFSLGSAAGAGIL 377 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 56.2 bits (134), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 40/128 (31%), Positives = 62/128 (48%), Gaps = 4/128 (3%) Query: 98 NIDRKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDH 157 DRKR++L ++ + A + LL+AR++ G+ G S+ A+A L H Sbjct: 83 RFDRKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLAIATDLFAPH 142 Query: 158 LFARAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPP 217 L R M +I T + + V PIG Y+ N W W F+A + L A + + R+ P Sbjct: 143 LRGRVMGVIQTSFAASQVLGIPIGLYLSNRWNWHVPFLAMAALGL---AGGLVVLVRMQP 199 Query: 218 KD-HPSLK 224 D H +LK Sbjct: 200 VDGHLTLK 207 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 963,040 Number of extensions: 31227 Number of successful extensions: 268 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 276 Number of HSP's successfully gapped: 16 Length of query: 422 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 334 Effective length of database: 1,203,190 Effective search space: 401865460 Effective search space used: 401865460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)