BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0484.p (206 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb... 400 e-113 gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 72 3e-14 gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 62 3e-11 gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herb... 62 5e-11 >gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 400 bits (1029), Expect = e-113, Method: Compositional matrix adjust. Identities = 203/206 (98%), Positives = 204/206 (99%) Query: 1 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAGAHKQPVFLALNPFGQVPVLEDGEV 60 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAGAHK+P FLALNPFGQVPVLEDGEV Sbjct: 1 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAGAHKKPAFLALNPFGQVPVLEDGEV 60 Query: 61 VIADSNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAF 120 VIADSNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAF Sbjct: 61 VIADSNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAF 120 Query: 121 NAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQG 180 NAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQG Sbjct: 121 NAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQG 180 Query: 181 FLQRIEALPGFVPFAQTPAGLMAAQA 206 FLQRIEALPGFVPFAQT AGLMAAQA Sbjct: 181 FLQRIEALPGFVPFAQTAAGLMAAQA 206 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 72.0 bits (175), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 53/190 (27%), Positives = 85/190 (44%), Gaps = 15/190 (7%) Query: 11 HAHRAVLFASLLGLPAELVEVDLAAGAHKQPVFLALNPFGQVPVL-----EDGE-VVIAD 64 + H+ + GLP +D+ AG P FL ++P ++P + DGE + + + Sbjct: 11 NGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGPDGEPISLFE 70 Query: 65 SNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAA-----RLVTVFGAA 119 S AIL+YLA K GR +L E + V +W+ G L GP RL Sbjct: 71 SGAILIYLAGKTGR--FLGETTREKYEVLQWVMFQMGGL--GPMLGQAHHFRLYAPEQIE 126 Query: 120 FNAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQ 179 + A L +++ L + +LA +H TIAD+A + + +DL +P V+ Sbjct: 127 YAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLDDFPNVK 186 Query: 180 GFLQRIEALP 189 + I A P Sbjct: 187 RWYDAISARP 196 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 62.4 bits (150), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 56/185 (30%), Positives = 83/185 (44%), Gaps = 22/185 (11%) Query: 23 GLPAELVEVDLAAGAHKQPVFLALNPFGQVPVLEDGE------VVIADSNAILVYLAKKA 76 GLP E V P FL+LNP ++P + D + + +S AIL+YLA+K Sbjct: 43 GLPYEAHLVSFETNDQMSPEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKT 102 Query: 77 GRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAFNAEEV---------IQ 127 G+ +LP DA V +W+ G GP +L F F +E + Sbjct: 103 GQ--FLPADAAARYQVIQWVMFQMG--GVGPMFGQLG--FFHRFAGKEYEDKRPRDRYVN 156 Query: 128 RAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQGFLQRIEA 187 + LLG +++ LA+ WL D +IAD+A + ++ R G + G Q F A Sbjct: 157 ESKRLLGVMDKRLAQSQWLGGDAYSIADIATFPWI-RNLIGFYEAADLVGFQDFPHVARA 215 Query: 188 LPGFV 192 L FV Sbjct: 216 LEAFV 220 >gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 61.6 bits (148), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 57/203 (28%), Positives = 92/203 (45%), Gaps = 6/203 (2%) Query: 1 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAGAHKQPVFLALNPFGQVPVLEDGEV 60 +KI+ S + + + + LG+ E + +P FLA+NP VPV+E+ Sbjct: 2 LKIYGRDSSINVRKVLWACAELGIAFEREDWGAGFRPTSEPAFLAMNPNAMVPVIEEDGF 61 Query: 61 VIADSNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGEL--AYGPAAARLVTVFGA 118 V+ +SNAI+ YLA + G + P AQ A V +W+ A +L A+ A LV A Sbjct: 62 VLWESNAIIRYLANRHGGAALYPLQAQARARVDQWIDWQASDLNRAWSYAYLTLVRKSPA 121 Query: 119 AFNAEEV---IQRAHLLLGRLEQHLAKR-DWLAADHPTIADVAVYSYLARAPEGNVDLRG 174 NA++V I + LEQ L ++A T+AD++V + R + Sbjct: 122 HGNAQQVAASIADWTRFMEVLEQQLQHSGGYVAGAEFTLADISVGLSVNRWFGTPFEHPP 181 Query: 175 YPGVQGFLQRIEALPGFVPFAQT 197 P V + R+ GF + + Sbjct: 182 LPAVAAYYDRLTERDGFRRYGRN 204 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 701,946 Number of extensions: 28766 Number of successful extensions: 117 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 4 Length of query: 206 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 125 Effective length of database: 1,236,335 Effective search space: 154541875 Effective search space used: 154541875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)