BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0478.p (484 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 985 0.0 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 490 e-139 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 375 e-105 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 337 1e-93 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 306 2e-84 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 985 bits (2547), Expect = 0.0, Method: Compositional matrix adjust. Identities = 482/484 (99%), Positives = 482/484 (99%) Query: 1 MLQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANA 60 MLQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANA Sbjct: 1 MLQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANA 60 Query: 61 AWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWF 120 AWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWF Sbjct: 61 AWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWF 120 Query: 121 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 180 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL Sbjct: 121 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 180 Query: 181 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKDIGGEMTSNPIVRKISFTGSTGVGK 240 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPK IGGEMTSNPIVRKISFTGSTGVGK Sbjct: 181 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGK 240 Query: 241 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD 300 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD Sbjct: 241 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD 300 Query: 301 GVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK 360 GVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK Sbjct: 301 GVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK 360 Query: 361 RHALGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEALALANDTEFGLASYFY 420 RHALGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEE LALANDTEFGLASYFY Sbjct: 361 RHALGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFY 420 Query: 421 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC Sbjct: 421 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 Query: 481 MGGI 484 MGGI Sbjct: 481 MGGI 484 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 490 bits (1261), Expect = e-139, Method: Compositional matrix adjust. Identities = 271/489 (55%), Positives = 352/489 (71%), Gaps = 9/489 (1%) Query: 2 LQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAA 61 L L+ L+ + W A G +L V +PA G++ VP GAA+ R A+EAA AA Sbjct: 4 LTLQRQDLMPGAQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAA 63 Query: 62 WPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFA 121 + AW+ AK+R+ I++RW +L+LA+ DDL +++ EQGKPLAEA+GE+ YAAS++EWF Sbjct: 64 FAAWRATPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFG 123 Query: 122 EEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLK 181 EE R GD IP+P R++ +KE +GV AAITPWNFPAAMI RK PALAAGC +V K Sbjct: 124 EEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCK 183 Query: 182 PAEATPFSALALAVLAERAGIPAGVFSVVTG----TPKDIGGEMTSNPIVRKISFTGSTG 237 PAE TP ++LAL LA+ AG+P GV ++VT TP+ + + ++ VRKISFTGST Sbjct: 184 PAEDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWL-ADGRVRKISFTGSTA 242 Query: 238 VGKLLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIY 297 VGK L + SA ++KKLSLELGGNAPFIVFDDAD+DAA++G +A+K+RN GQTCV NRIY Sbjct: 243 VGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIY 302 Query: 298 VQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLL 357 VQ+ VYDAF KL V KVG T+ GP+IN +A+ K++QHV DA+A+GARV+ Sbjct: 303 VQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVIT 362 Query: 358 GGKR---HALG-HSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEALALANDTEF 413 GGKR G +++ PTVLADVT AMQ + EETFGP+AP+ RF T++E +A AN T F Sbjct: 363 GGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPF 422 Query: 414 GLASYFYSRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDY 473 GLA+YFYS D+ RI RV + LESG+VG+N G ++ E APFGGVK+SG GREGS +G+DDY Sbjct: 423 GLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDY 482 Query: 474 LVVKYLCMG 482 L KY+C G Sbjct: 483 LHTKYVCQG 491 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 375 bits (964), Expect = e-105, Method: Compositional matrix adjust. Identities = 200/469 (42%), Positives = 288/469 (61%), Gaps = 2/469 (0%) Query: 13 QAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKE 72 Q F+NG+W + G + V NPA E++G + A+ RA+EAA+ + W+ +A E Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 73 RSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI 132 RS I+RR +L+ +++A +MT EQGKPLAEA+ E AA I+WFAEE +RTYG + Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 133 PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALA 192 P+ P +VIKE +G AA TPWNFP + RK ALAAGC +++K E TP S Sbjct: 126 PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAE 185 Query: 193 LAVLAERAGIPAGVFSVVTGTPKDIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKK 252 L AG+PAGV ++V G P +I + +P+++KISFTGST VGK L + +K+ Sbjct: 186 LIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKR 245 Query: 253 LSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVE 312 ++ELGG+AP +VFDDAD+D A + +K+RNAGQ CV R VQ GV++AF K V Sbjct: 246 ATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVT 305 Query: 313 AVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPT 372 ++ KVG G E G+T GP+ N++ + +E + DAV++GA++ GGKR FF+PT Sbjct: 306 YAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPT 365 Query: 373 VLADVTPAMQVAREETFGPMAPLFRFKTDEEALALANDTEFGLASYFYSRDIGRIWRVAE 432 VL ++ + EE FGP+A + F+T +E + AN +GLA+Y +++ +A Sbjct: 366 VLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAA 425 Query: 433 GLESGMVGIN-TGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 +ESGM+ IN GL E+ PFGG+K SG G EG I+ Y+ K + Sbjct: 426 RVESGMLTINHLGLALPEL-PFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 337 bits (864), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 190/468 (40%), Positives = 285/468 (60%) Query: 13 QAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKE 72 Q +++G++ + V +PA G++LG +P A+ AI+AA+ A+ W++ + E Sbjct: 6 QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLE 65 Query: 73 RSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI 132 RS ILRR L +A +T + GKPLA+A EI AA ++W AEEG+R YG + Sbjct: 66 RSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVV 125 Query: 133 PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALA 192 + P R +V++E +GVCAAI+PWNFP +K A+AAGC +VLK +P + + Sbjct: 126 GARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVL 185 Query: 193 LAVLAERAGIPAGVFSVVTGTPKDIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKK 252 +A L AG+PAGVF++V+G +I + +P+VR ISFTGS VG L Q+A +K+ Sbjct: 186 MAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKR 245 Query: 253 LSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVE 312 +S+ELGG+AP +VFDDA+++ A K RNAGQ CV +R YVQ G+Y F E Sbjct: 246 MSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFAE 305 Query: 313 AVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPT 372 A+ + KVG G E GV GPLI+ + + V+ + DA+A+G+ ++ GG R F+ PT Sbjct: 306 ALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGHFYAPT 365 Query: 373 VLADVTPAMQVAREETFGPMAPLFRFKTDEEALALANDTEFGLASYFYSRDIGRIWRVAE 432 ++ V P+ +V +EE FGP+A + F EEA+A+AN GLASY +++ + RV+ Sbjct: 366 LITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTAHRVSR 425 Query: 433 GLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 L++GMV IN +++ PFGG+ +SG G EG G + +LV K + Sbjct: 426 DLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMIS 473 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 306 bits (785), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 177/469 (37%), Positives = 265/469 (56%), Gaps = 14/469 (2%) Query: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 +++G+W ER AV PA E + + L AA+ +A++AA A PAW + ER+ Sbjct: 19 YIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLERA 78 Query: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI-- 132 A+L R + L+L ++ A +T E G P I YA + A E R D + Sbjct: 79 ALLGRVHALLLERSELFAQALTLEMGAP-------ISYARTAHVPLAAEHLRVARDNLAD 131 Query: 133 -PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSAL 191 P P +++E IGVCA ITPWN+P IT K GPALAAGC +VLKP+E +P SAL Sbjct: 132 YPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSAL 191 Query: 192 ALAVLAERAGIPAGVFSVVTGTPKDIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIK 251 A + AG+PAGVF++V G+ ++G ++++P V IS TGST G L+ Q +A ++K Sbjct: 192 LFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVK 251 Query: 252 KLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLV 311 +++ ELGG +P +V DADL+ A+ +A+ RN GQ+C R+ V + Sbjct: 252 RVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELAL 311 Query: 312 EAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLG--GKRHALGHSFF 369 + + KVG + T GPL N +V+Q + + GAR+L G G+ L ++ Sbjct: 312 ATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYY 371 Query: 370 -EPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEALALANDTEFGLASYFYSRDIGRIW 428 PT+ ++V M +A+EE FGP+ + + T EEA+A+ANDT +GL ++ RD ++ Sbjct: 372 ARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVR 431 Query: 429 RVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVK 477 VA ++SG V +N + APFGG KQSG GRE G+++Y+ VK Sbjct: 432 AVAARIQSGQVHLNYPAWDPQ-APFGGYKQSGNGREYGVEGMEEYMEVK 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,510,691 Number of extensions: 65457 Number of successful extensions: 330 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 281 Number of HSP's successfully gapped: 21 Length of query: 484 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 395 Effective length of database: 1,198,455 Effective search space: 473389725 Effective search space used: 473389725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)