BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0477.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310411|ref|YP_003774503.1| spermidine/putrescine ABC trans... 543 e-156 gi|300311432|ref|YP_003775524.1| molybdate ABC transporter perme... 70 2e-13 gi|300310985|ref|YP_003775077.1| sulfate ABC transporter permeas... 64 2e-11 gi|300309935|ref|YP_003774027.1| spermidine/putrescine ABC trans... 62 8e-11 gi|300312637|ref|YP_003776729.1| sugar ABC transporter permease ... 61 9e-11 >gi|300310411|ref|YP_003774503.1| spermidine/putrescine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 277 Score = 543 bits (1399), Expect = e-156, Method: Compositional matrix adjust. Identities = 277/277 (100%), Positives = 277/277 (100%) Query: 24 MNKPPLFPPYTSLVERAWFFASRGFNLLVLLFLVSPILVMIPLSFSDSSFLMYPIKSFSL 83 MNKPPLFPPYTSLVERAWFFASRGFNLLVLLFLVSPILVMIPLSFSDSSFLMYPIKSFSL Sbjct: 1 MNKPPLFPPYTSLVERAWFFASRGFNLLVLLFLVSPILVMIPLSFSDSSFLMYPIKSFSL 60 Query: 84 RWYENLFSSDDWVRAAQNSFIVAPAATVIATVLGTLAAVGLNKADFRGKGILMAILISPM 143 RWYENLFSSDDWVRAAQNSFIVAPAATVIATVLGTLAAVGLNKADFRGKGILMAILISPM Sbjct: 61 RWYENLFSSDDWVRAAQNSFIVAPAATVIATVLGTLAAVGLNKADFRGKGILMAILISPM 120 Query: 144 IVPVVVVGVGVYLFFARIGLSDSYLGLILAHAALGAPFVVTTVLATLQGFNHNLVRASLS 203 IVPVVVVGVGVYLFFARIGLSDSYLGLILAHAALGAPFVVTTVLATLQGFNHNLVRASLS Sbjct: 121 IVPVVVVGVGVYLFFARIGLSDSYLGLILAHAALGAPFVVTTVLATLQGFNHNLVRASLS 180 Query: 204 LGASPLRTFFRVTLPVIAPGLISGALFAFATSFDEVVLTLFVAGPEQATLPRQMFAGIKD 263 LGASPLRTFFRVTLPVIAPGLISGALFAFATSFDEVVLTLFVAGPEQATLPRQMFAGIKD Sbjct: 181 LGASPLRTFFRVTLPVIAPGLISGALFAFATSFDEVVLTLFVAGPEQATLPRQMFAGIKD 240 Query: 264 NISPTIAALATILIIFSTCLLLALEWLRGRNKAAARF 300 NISPTIAALATILIIFSTCLLLALEWLRGRNKAAARF Sbjct: 241 NISPTIAALATILIIFSTCLLLALEWLRGRNKAAARF 277 >gi|300311432|ref|YP_003775524.1| molybdate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 224 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 59/201 (29%), Positives = 99/201 (49%), Gaps = 21/201 (10%) Query: 102 SFIVAPAATVIATVLGTLAAVGLNKADFRGKGILMAILISPMIVPVVVVGVGVYLFFARI 161 S VA AT++ VLG AA GL++ + + ++L P+++P V+G + + F R Sbjct: 11 SLKVAGLATLLNLVLGVAAAYGLSRWRSALRDFIDSVLTLPLVLPPTVLGYYLLVLFGRR 70 Query: 162 GLSDSYL------------GLILAHAALGAPFVVTTVLATLQGFNHNLVRASLSLGASPL 209 G ++L G +LA + P V+ + A + +H + A+ LG S Sbjct: 71 GALGAWLERMGIELVFTWQGAVLASTVVAFPLVLKSARAAFESVDHQMEDAARVLGVSEA 130 Query: 210 RTFFRVTLPVIAPGLISGALFAFATSFDEVVLTLFVAGPEQATLP---RQMFAGIKDNIS 266 FFRV+LP+ G+++G L AFA + E TL VAG LP + + I + + Sbjct: 131 GVFFRVSLPLAMRGIMAGVLLAFARALGEFGATLMVAG----NLPGRTQTLSVAIYEAVQ 186 Query: 267 PTIAALATILIIFS--TCLLL 285 AT+L++ + TC++L Sbjct: 187 AGDDGSATLLVVITSITCIVL 207 >gi|300310985|ref|YP_003775077.1| sulfate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 299 Score = 63.5 bits (153), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 64/227 (28%), Positives = 102/227 (44%), Gaps = 19/227 (8%) Query: 91 SSDDWVRAAQNSFIVAPAATVIATVLGTLAAVGLNKADFRGKGILMAILISPMIVPVVVV 150 +D W A + + I A A + V G AA + K +FRGK +L+ ++ P V V+ Sbjct: 72 EADAW-SAIKLTLITAAIAVPLNLVFGVAAAWAIAKFEFRGKNLLLTLIDLPFSVSPVIS 130 Query: 151 GVGVYLFFARIGLSDSYL------------GLILAHAALGAPFVVTTVLATLQGFNHNLV 198 G+ L F G +L G++LA + PFV ++ +Q Sbjct: 131 GLIYVLLFGLQGYLGPWLREHDIKILFAVPGIVLATIFVTFPFVARELIPLMQSQGSEEE 190 Query: 199 RASLSLGASPLRTFFRVTLPVIAPGLISGALFAFATSFDEVVLTLFVAGP---EQATLPR 255 A+L LGAS RTF+ VTLP I GL+ G + A + E V+G E T+P Sbjct: 191 EAALVLGASGWRTFWHVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGETNTIPL 250 Query: 256 QMFAGIKD-NISP--TIAALATILIIFSTCLLLALEWLRGRNKAAAR 299 Q+ + NI+ +A+L L + + + +EW + +A+ Sbjct: 251 QVEILYNEFNITGAFAVASLLAFLALVTLAIKSVIEWRLHQQHSASE 297 >gi|300309935|ref|YP_003774027.1| spermidine/putrescine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 61.6 bits (148), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 70/262 (26%), Positives = 115/262 (43%), Gaps = 26/262 (9%) Query: 42 FFASRGFNLLVLLFLVSPILVMIPLSFSDSSFLMYPIKSFSLRWYEN---LFSSDDWVRA 98 F+ GF LLV FLV P+ V+ L+ +F + +LRW ++ W Sbjct: 7 FYLQLGFTLLVCAFLVVPV-VLSMLAGVTENFFVGLSSGLTLRWVAQVWEMYQPTIW--- 62 Query: 99 AQNSFIVAPAATVIATVLGTLAAVGLNKADFRGKG-----ILMAILISPMIVPVVVVGVG 153 S +A A + G A L A +RG ++ +L+ P+ VP + + Sbjct: 63 --RSLFIALACLAVTLAAGVPLAYML--ARYRGGNSRLARVIEELLMMPVAVPGLATALA 118 Query: 154 VYLFFARIGLSDSYLGLIL-AHAALGAPFVVTTVLATLQGFN-HNLVRASLSLGASPLRT 211 + + + + + L IL H PF+V VLA +Q L A+ SLGA +R Sbjct: 119 LIMAYGQYREFRASLWFILVGHVIFTLPFMVRPVLAVMQSSQLAQLEEAAASLGAGRMRR 178 Query: 212 FFRVTLPVIAPGLISGALFAFATSFDEVVLTLFVAGPEQATLPRQMFAGIKD---NISPT 268 FF + +P A G+++GAL S E +T + P TLP G+ D ++ Sbjct: 179 FFGIVIPNAASGILAGALMVVTLSIGEFNITWMLHTPLTQTLP----VGLADAYASMRLE 234 Query: 269 IAALATILIIFSTC-LLLALEW 289 + + T++ F LL+ ++W Sbjct: 235 VGSAYTLIFFFMIIPLLIGMQW 256 >gi|300312637|ref|YP_003776729.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 61.2 bits (147), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 44/160 (27%), Positives = 76/160 (47%), Gaps = 11/160 (6%) Query: 101 NSFIVAPAATVIATVLGTLAAVGLNKADFRGK-----GILMAILISPMIVPVVVVGVGVY 155 N+ IV+ +T + AA + + F+G GI +A LI P I+ + + + Sbjct: 89 NTVIVSVVSTFASLAASVFAAYAIERLRFQGSKQVGLGIFLAYLIPPSILFIPLAAI--- 145 Query: 156 LFFARIGLSDSYLGLILAHAALGAPFVVTTVLATLQGFNHNLVRASLSLGASPLRTFFRV 215 ++GL D+ LIL + PF ++ + + L +L GA+ ++ Sbjct: 146 --VFKLGLFDTRWALILTYPTFLIPFCTWLLMGYFRSIPYELEECALIDGATRWEILVKI 203 Query: 216 TLPVIAPGLISGALFAFATSFDEVVLTL-FVAGPEQATLP 254 LP+ PGLIS +FAF S++E + L F++ E T+P Sbjct: 204 ILPLAVPGLISAGIFAFTLSWNEFIYALTFISSSEVKTVP 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 703,631 Number of extensions: 21836 Number of successful extensions: 129 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 125 Number of HSP's successfully gapped: 19 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)