BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0473.p (376 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310414|ref|YP_003774506.1| spermidine/putrescine ABC trans... 774 0.0 gi|300312634|ref|YP_003776726.1| sugar ABC transporter ATPase [H... 258 4e-70 gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC trans... 255 4e-69 gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC trans... 240 1e-64 gi|300309785|ref|YP_003773877.1| sugar ABC transporter ATPase [H... 237 1e-63 >gi|300310414|ref|YP_003774506.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 774 bits (1998), Expect = 0.0, Method: Compositional matrix adjust. Identities = 375/376 (99%), Positives = 375/376 (99%) Query: 1 MMAAYAMKQAAQESVQQRDVLVQFCGVKKTYDGENLIVKNLDLDIRRGEFLTLLGPSGSG 60 MMAAYAMKQAAQESVQQ DVLVQFCGVKKTYDGENLIVKNLDLDIRRGEFLTLLGPSGSG Sbjct: 1 MMAAYAMKQAAQESVQQSDVLVQFCGVKKTYDGENLIVKNLDLDIRRGEFLTLLGPSGSG 60 Query: 61 KTTCLMMLAGFEFPTGGEIRLDGQLLNRVPPHKRNIGMVFQNYALFPHMTIAQNVAYPLS 120 KTTCLMMLAGFEFPTGGEIRLDGQLLNRVPPHKRNIGMVFQNYALFPHMTIAQNVAYPLS Sbjct: 61 KTTCLMMLAGFEFPTGGEIRLDGQLLNRVPPHKRNIGMVFQNYALFPHMTIAQNVAYPLS 120 Query: 121 VRKMDRATREAKVKQALDMVQMGKFGDRYPTQLSGGQQQRVALARTLVFDPQLVLMDEPL 180 VRKMDRATREAKVKQALDMVQMGKFGDRYPTQLSGGQQQRVALARTLVFDPQLVLMDEPL Sbjct: 121 VRKMDRATREAKVKQALDMVQMGKFGDRYPTQLSGGQQQRVALARTLVFDPQLVLMDEPL 180 Query: 181 GALDKQLREHMQLELKALHKRLGVTFVYVTHDQSEALTMSDRVAVFDQGVIQQLAPVTEL 240 GALDKQLREHMQLELKALHKRLGVTFVYVTHDQSEALTMSDRVAVFDQGVIQQLAPVTEL Sbjct: 181 GALDKQLREHMQLELKALHKRLGVTFVYVTHDQSEALTMSDRVAVFDQGVIQQLAPVTEL 240 Query: 241 YEYPDNQFVANFIGDNNRFKGSVESVQGEHCTVRLTDGAALTGLNVHRAHAGQSITACVR 300 YEYPDNQFVANFIGDNNRFKGSVESVQGEHCTVRLTDGAALTGLNVHRAHAGQSITACVR Sbjct: 241 YEYPDNQFVANFIGDNNRFKGSVESVQGEHCTVRLTDGAALTGLNVHRAHAGQSITACVR 300 Query: 301 PERIRLAAGGSAEPNSIRASIAGLIYFGDHVRVRCTVAGQEDCFVKLSLSDPALSGLSEG 360 PERIRLAAGGSAEPNSIRASIAGLIYFGDHVRVRCTVAGQEDCFVKLSLSDPALSGLSEG Sbjct: 301 PERIRLAAGGSAEPNSIRASIAGLIYFGDHVRVRCTVAGQEDCFVKLSLSDPALSGLSEG 360 Query: 361 SSVVLHVAQDRLRCFL 376 SSVVLHVAQDRLRCFL Sbjct: 361 SSVVLHVAQDRLRCFL 376 >gi|300312634|ref|YP_003776726.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 258 bits (660), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 139/300 (46%), Positives = 186/300 (62%), Gaps = 20/300 (6%) Query: 22 VQFCGVKKTYDGENLIVKNLDLDIRRGEFLTLLGPSGSGKTTCLMMLAGFEFPTGGEIRL 81 VQ VKK + G I++ +D+DI GEF L+GPSG GK+T L MLAG E TGGEI + Sbjct: 4 VQIRAVKKQF-GSTQIIRGVDIDIADGEFTVLVGPSGCGKSTLLRMLAGLEEITGGEILI 62 Query: 82 DGQLLNRVPPHKRNIGMVFQNYALFPHMTIAQNVAYPLSVRKMDRATREAKVKQALDMVQ 141 G ++N V P R+I MVFQNYAL+PHMT+ N+A+ L++ K D+A + +VK+A D++ Sbjct: 63 GGTVVNNVQPKDRDIAMVFQNYALYPHMTVRDNMAFSLTLAKKDKAFVDERVKKAADILG 122 Query: 142 MGKFGDRYPTQLSGGQQQRVALARTLVFDPQLVLMDEPLGALDKQLREHMQLELKALHKR 201 + + DRYP QLSGGQ+QRVA+ R +V DPQ+ L DEPL LD +LR M+ E+K LH+R Sbjct: 123 LNQLLDRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEPLSNLDAKLRVQMRTEIKELHQR 182 Query: 202 LGVTFVYVTHDQSEALTMSDRVAVFDQGVIQQLAPVTELYEYPDNQFVANFIGD------ 255 L T +YVTHDQ EA+TM+D++ V G+++Q +LY+YP N FVA FIG Sbjct: 183 LKTTSIYVTHDQIEAMTMADQIVVMRDGLVEQRGRPLDLYDYPANLFVAGFIGSPAMNFI 242 Query: 256 -----NNRFKGSVESVQGEHCTVRLTDGAALTGLNVHRAHAGQSITACVRPERIRLAAGG 310 N VE G GAAL G + GQ +T VRPE + + A G Sbjct: 243 PATLRRNATGAEVEFADGTRVPAPY--GAALQG------NDGQKVTYGVRPEHLSIGAAG 294 >gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 362 Score = 255 bits (651), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 133/308 (43%), Positives = 191/308 (62%), Gaps = 11/308 (3%) Query: 35 NLIVKNLDLDIRRGEFLTLLGPSGSGKTTCLMMLAGFEFPTGGEIRL------DGQLLNR 88 N I+K + ++++RGE + LLGPSGSGKTT L +AG E P G I + DG Sbjct: 20 NPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPKAGAIDIGKRRVFDGARNFE 79 Query: 89 VPPHKRNIGMVFQNYALFPHMTIAQNVAYPLSVRKMDRATREAKVKQALDMVQMGKFGDR 148 +P +RN+G+VFQ+YAL+PH T+A NVAY L +RK +VK L + +G G+R Sbjct: 80 MPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSGEIATRVKAVLSQLGLGHLGER 139 Query: 149 YPTQLSGGQQQRVALARTLVFDPQLVLMDEPLGALDKQLREHMQLELKALHKRLGVTFVY 208 YP QLSGGQQQRVA+AR LV++PQ++L+DEPL LD +LRE + L+ L RLG++ + Sbjct: 140 YPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLREEARAFLRELIVRLGLSALM 199 Query: 209 VTHDQSEALTMSDRVAVFDQGVIQQLAPVTELYEYPDNQFVANFIGDNNRFKGSVESVQG 268 VTHDQ EA+ +SDR+ + + G I+Q +Y+ P+ F A F+G NN+ V S +G Sbjct: 200 VTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFTAEFMGSNNKLAAEVLSREG 259 Query: 269 EHCTVRLTDGAALTGLNVHR-AHAGQSITACVRPERIRLAAGGSAEPNSIRASIAGLIYF 327 E TV+L G A+ L G+ +T +R E++R++ G A N+IR ++ +Y Sbjct: 260 ERVTVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRIS--GEASDNAIRLPLSTCMYL 317 Query: 328 GDHVRVRC 335 GD R C Sbjct: 318 GD--RWEC 323 >gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 240 bits (612), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 131/336 (38%), Positives = 190/336 (56%), Gaps = 13/336 (3%) Query: 16 QQRDVLVQFCGVKKTYDGENLIVKNLDLDIRRGEFLTLLGPSGSGKTTCLMMLAGFEFP- 74 Q V ++ K++ ++ LDL I GE L LLGPSG GKTT L M+AG EFP Sbjct: 4 HQEAVSIRLSQCAKSFANGTRALQPLDLQIHPGETLVLLGPSGCGKTTTLRMIAGLEFPD 63 Query: 75 TGGEIRLDGQLLNRVPPHKRNIGMVFQNYALFPHMTIAQNVAYPLSVRKMDRATREAKVK 134 GG + + + +P KR +GMVFQNYALFP+M++ +N+AY + +RK+ R +V+ Sbjct: 64 EGGRVLFGEEDVTMLPIEKRGVGMVFQNYALFPNMSVGENIAYGMKIRKIAAGERAERVE 123 Query: 135 QALDMVQMGKFGDRYPTQLSGGQQQRVALARTLVFDPQLVLMDEPLGALDKQLREHMQLE 194 + L+MV + R QLSGGQ+QRVALAR L +P+++L+DEPL ALD +LRE ++ + Sbjct: 124 RLLEMVHLQGLSHRRVDQLSGGQKQRVALARALAMEPRVLLLDEPLTALDAKLREAVRSD 183 Query: 195 LKALHKRLGVTFVYVTHDQSEALTMSDRVAVFDQGVIQQLAPVTELYEYPDNQFVANFIG 254 L L + LG+T +YVTHDQ EA+ + DR+ V ++G I Q+ E+Y +P N FVA FIG Sbjct: 184 LNKLLRSLGITAIYVTHDQGEAMALGDRIVVMERGKISQIGTPQEIYYHPANDFVAEFIG 243 Query: 255 DNNRFKGSV--ESVQGEHCTVRLTDGAALTGLNVHRAHAGQSITACVRPERIRLAAGGSA 312 NR G V + +Q T+ L A AGQ A RPE + + A Sbjct: 244 AMNRVDGIVAGDRLQLPTGTLPLPPDAV----------AGQPAQALFRPEDVEVIDITEA 293 Query: 313 EPNSIRASIAGLIYFGDHVRVRCTVAGQEDCFVKLS 348 + + +R + + GD R + + + S Sbjct: 294 DAHCLRGKLINTFFLGDRTRFEIEIGAERPVIAETS 329 >gi|300309785|ref|YP_003773877.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 372 Score = 237 bits (604), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 136/361 (37%), Positives = 203/361 (56%), Gaps = 19/361 (5%) Query: 22 VQFCGVKKTYDGENLIVKNLDLDIRRGEFLTLLGPSGSGKTTCLMMLAGFEFPTGGEIRL 81 V + K YD +N ++++++L+I GEF+ +GPSG GK+T L M+AG E + G++ + Sbjct: 4 VSIRNLAKRYD-DNEVMRDINLEIEDGEFVVFVGPSGCGKSTLLRMIAGLEEISDGDLDI 62 Query: 82 DGQLLNRVPPHKRNIGMVFQNYALFPHMTIAQNVAYPLSVRKMDRATREAKVKQALDMVQ 141 + +N VP KR + MVFQ+YAL+PHM++ N+A+ L + +A +A V+ A ++ Sbjct: 63 GARRMNEVPASKRGVAMVFQSYALYPHMSLYDNMAFGLKIAGKSKAEIDAAVQHAAKILH 122 Query: 142 MGKFGDRYPTQLSGGQQQRVALARTLVFDPQLVLMDEPLGALDKQLREHMQLELKALHKR 201 + DR P LSGGQ+QRVA+ R + P + L DEPL LD LR M+LE LH Sbjct: 123 IDHLLDRKPRALSGGQRQRVAIGRAITRQPSVFLFDEPLSNLDAALRVKMRLEFAKLHDD 182 Query: 202 LGVTFVYVTHDQSEALTMSDRVAVFDQGVIQQLAPVTELYEYPDNQFVANFIGDN--NRF 259 L T +YVTHDQ EA+T++D++ V +G I+Q+ +LY +P N+FVA FIG N Sbjct: 183 LKTTMIYVTHDQIEAMTLADKIVVLSEGRIEQVGSPQQLYHHPANRFVAGFIGSPKMNFI 242 Query: 260 KGSVESVQGEHCTVRLTDG----AALTGLNVHRAHAGQSITACVRPERIRLAAGGSAEPN 315 G+V ++Q + V+L G AA+ G + GQ +T VRPE + +A G +A Sbjct: 243 DGTVAAIQADGVQVQLPGGGLQWAAVDGSTL---QVGQKVTLGVRPEHLNIAQGQAA--- 296 Query: 316 SIRASIAGLIYFGDHVRVRCTVAGQEDCFVKLSLSDPALSGLSEGSSVVLHVAQDRLRCF 375 ++A L GD + G ED + L + D L VL +A D RC Sbjct: 297 -LQARCTALELLGDFSYLYAAYEGSEDALI-LRVPD----SLDAPHGSVLPLAADPARCH 350 Query: 376 L 376 L Sbjct: 351 L 351 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,069,675 Number of extensions: 43522 Number of successful extensions: 538 Number of sequences better than 1.0e-08: 132 Number of HSP's gapped: 326 Number of HSP's successfully gapped: 146 Length of query: 376 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 289 Effective length of database: 1,207,925 Effective search space: 349090325 Effective search space used: 349090325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)