BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0471.p (516 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310416|ref|YP_003774508.1| MocR family transcription regul... 1054 0.0 gi|300311074|ref|YP_003775166.1| transcription regulator protein... 133 4e-32 gi|300311134|ref|YP_003775226.1| transcription regulator protein... 130 4e-31 gi|300313928|ref|YP_003778020.1| GntR family transcription regul... 127 1e-30 gi|300311139|ref|YP_003775231.1| MocR family transcription regul... 127 2e-30 >gi|300310416|ref|YP_003774508.1| MocR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 516 Score = 1054 bits (2725), Expect = 0.0, Method: Compositional matrix adjust. Identities = 513/516 (99%), Positives = 514/516 (99%) Query: 1 LKIASLSDYLLLRINRPAAKAAPSKAKGGSAPESGAAKPAPRTPVNRQIYQLIREAILAH 60 +KIASLSDYLLLRINRPAAKAAP KAK GSAPESGAAKPAPRTPVNRQIYQLIREAILAH Sbjct: 1 MKIASLSDYLLLRINRPAAKAAPGKAKAGSAPESGAAKPAPRTPVNRQIYQLIREAILAH 60 Query: 61 TLPAGMQLPSSRDLALELGSSRNTVTYAYEQLLAEGYLESRPGAGTFVADTAPDQIPEAV 120 TLPAGMQLPSSRDLALELGSSRNTVTYAYEQLLAEGYLESRPGAGTFVADTAPDQIPEAV Sbjct: 61 TLPAGMQLPSSRDLALELGSSRNTVTYAYEQLLAEGYLESRPGAGTFVADTAPDQIPEAV 120 Query: 121 ERPEPLTDPSGKSELSARGAMLTRQAGVGERQWGAFMPGVPDVTCVPYKIWGRLQNKHWR 180 ERPEPLTDPSGKSELSARGAMLTRQAGVGERQWGAFMPGVPDVTCVPYKIWGRLQNKHWR Sbjct: 121 ERPEPLTDPSGKSELSARGAMLTRQAGVGERQWGAFMPGVPDVTCVPYKIWGRLQNKHWR 180 Query: 181 RSNADLLTYGPGAGYAGLREQIAEYLRVARSVNCTPSQVLITTGIHQSLDIVVKLLGEHG 240 RSNADLLTYGPGAGYAGLREQIAEYLRVARSVNCTPSQVLITTGIHQSLDIVVKLLGEHG Sbjct: 181 RSNADLLTYGPGAGYAGLREQIAEYLRVARSVNCTPSQVLITTGIHQSLDIVVKLLGEHG 240 Query: 241 DSAWVEDPCYWGTRSVLNSLGIQSVPIAVDQEGMRMRLANLRRPPRFICTTPSHQYPLGM 300 DSAWVEDPCYWGTRSVLNSLGIQSVPIAVDQEGMRMRLANLRRPPRFICTTPSHQYPLGM Sbjct: 241 DSAWVEDPCYWGTRSVLNSLGIQSVPIAVDQEGMRMRLANLRRPPRFICTTPSHQYPLGM 300 Query: 301 VMSLSRRRMLLEYAATHKVWIIEDDYDSEFRYGGRPLASLQGMDTQDRVLYMGTFSKIMF 360 VMSLSRRRMLLEYAATHKVWIIEDDYDSEFRYGGRPLASLQGMDTQDRVLYMGTFSKIMF Sbjct: 301 VMSLSRRRMLLEYAATHKVWIIEDDYDSEFRYGGRPLASLQGMDTQDRVLYMGTFSKIMF 360 Query: 361 PGLRIGFLVVPESLARAFSTGIAELYRNGQVFLQATLADFMAEGHFASHIRKMRVLYAER 420 PGLRIGFLVVPESLARAFSTGIAELYRNGQVFLQATLADFMAEGHFASHIRKMRVLYAER Sbjct: 361 PGLRIGFLVVPESLARAFSTGIAELYRNGQVFLQATLADFMAEGHFASHIRKMRVLYAER 420 Query: 421 LQLLQQSINRHFGEKMTITGGEAGLHLVLGLPQECDDVLICEQALAAGIVVRPLSRYYMH 480 LQLLQQSINRHFGEKMTITGGEAGLHLVLGLPQECDDVLICEQALAAGIVVRPLSRYYMH Sbjct: 421 LQLLQQSINRHFGEKMTITGGEAGLHLVLGLPQECDDVLICEQALAAGIVVRPLSRYYMH 480 Query: 481 ARGARRGLLLGYASVPNDEIARAFDKLAAVIKPHLA 516 ARGARRGLLLGYASVPNDEIARAFDKLAAVIKPHLA Sbjct: 481 ARGARRGLLLGYASVPNDEIARAFDKLAAVIKPHLA 516 >gi|300311074|ref|YP_003775166.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 516 Score = 133 bits (334), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 135/510 (26%), Positives = 234/510 (45%), Gaps = 51/510 (10%) Query: 30 SAPESGAAKPAPRTPVNRQIYQLIREAILAHTLPAGMQLPSSRDLALELGSSRNTVTYAY 89 SAPESG R P+ R++ + AI A TL AG ++PS RDL + S +T Sbjct: 33 SAPESG------RQPLYRRLADHYQSAIQAGTLVAGDRMPSVRDLMRQHQVSLSTALQTL 86 Query: 90 EQLLAEGYLESRPGAGTFVADTAPDQIPEAVERPEPLTDPSGKSELSARGAMLTRQAGVG 149 + G+LE+RP +G FV I P+ +P + A G + V Sbjct: 87 RHMEEGGWLEARPRSGYFVRQPRRSAI-------RPVQEPKSLLAIDA-GQFVGIHERVS 138 Query: 150 E----RQWGAFMPGVPDVTCVP--YKIWGRLQNKHWR--RSNADLLTYG-PGAGYAGLRE 200 + RQ GA + +TC P Y + L+ R R +LLT P G A R+ Sbjct: 139 KYIALRQAGAPHIDLSGMTCAPQLYAV-ETLKQAAMRALRERPELLTSAMPHNGNATFRQ 197 Query: 201 QIAEYLRVARSVNC----TPSQVLITTGIHQSLDIVVKLLGEHGDSAWVEDPCYWGTRSV 256 +A R++N +V++T G ++L++ ++ + GD+ VE P Y+G Sbjct: 198 AVAR-----RALNAGMRIDTEEVVVTHGCIEALNLAMRAVAGPGDTIAVESPTYFGLLQA 252 Query: 257 LNSLGIQSVPI------AVDQEGMRMRL---ANLRRPPRFICTTPSHQYPLGMVMSLSRR 307 L +LG++++ I + E + + + N++ + P+ Q PLG +M + Sbjct: 253 LENLGMKALEIPTSPHTGISVEALELAVRTYGNIK----AVVVVPNLQNPLGCIMPEDHK 308 Query: 308 RMLLEYAATHKVWIIEDDYDSEFRYGGRPLASLQGMDTQDRVLYMGTFSKIMFPGLRIGF 367 L+ K+ +IEDD +E G +SL+ D V+Y + +K++ PG+R+G+ Sbjct: 309 DQLVRLCDQLKLPLIEDDAYTELADGSVTPSSLKSRDRNGNVIYCASLNKVLAPGMRLGW 368 Query: 368 LVVPESLARAFSTGIAELYRNGQVFLQATLADFMAEGHFASHIRKMRVLYAERLQLLQQS 427 + R R + + Q T ADF+A + H+R++R + ++ + + +S Sbjct: 369 MNGGRWHERVKMLKFTH-TRANEEWTQVTAADFIASPAYDRHLRRLRQMLKQQRERMAES 427 Query: 428 INRHFGEKMTITGGEAGLHLVLGLPQECDDVLICEQALAAGIVVRPLSRYYMHAR-GARR 486 I +F E + G+ L + LP + + E+ALA G+ V P + + +R G Sbjct: 428 IAAYFPEGTRLNLPNGGVGLWVELPVQVCSQQLFERALAEGVGVSPGAIFSNSSRYGHFV 487 Query: 487 GLLLGYASVPNDEIARAFDKLAAVIKPHLA 516 + G+ N E+ +A +L ++ HLA Sbjct: 488 RICCGHPF--NRELDQALRRLGGLVH-HLA 514 >gi|300311134|ref|YP_003775226.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 500 Score = 130 bits (326), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 122/445 (27%), Positives = 208/445 (46%), Gaps = 34/445 (7%) Query: 48 QIYQLIREAILAHTLPAGMQLPSSRDLALELGSSRNTVTYAYEQLLAEGYLESRPGAGTF 107 QI + IR I L +GM++PS R A SR TV AY++L+A+GYLESR G+G + Sbjct: 43 QIVRGIRARIDDKLLRSGMRMPSIRQFADTNQISRFTVVEAYDRLVAQGYLESRRGSGFY 102 Query: 108 VADTAPDQIPEAVERPEPLTDPSGKSELSARGAMLTRQAGVGERQWGAFMPGVPDVTCVP 167 V + AP + E T+P ++ + RQ + G +P P Sbjct: 103 VKERAPLGGSYGAAQAELDTEPGRGLDVVWLVRNMFRQMPPAKMPGGGLLP--------P 154 Query: 168 YKIWGRLQNKHWRRSNAD----LLTYGPGAGYAGLREQIAEYLRVAR-SVNCTPSQVLIT 222 + G L R + D LL YG G+ LR+Q+ L++A + P Q+++T Sbjct: 155 DWLDGELIANALRAVSRDNPQLLLGYGTPQGFLPLRQQLQ--LKLAELEIAAAPEQIVLT 212 Query: 223 TGIHQSLDIVVKLLGEHGDSAWVEDPCYWGTRSVLNSLGIQSVPIAVDQEG---MRMR-L 278 TG+ Q LD V + GD +V+DP ++ +LG + V I +G R+R L Sbjct: 213 TGVTQGLDTVARHFLRPGDVIFVDDPAWFLMFGSFAALGAKVVGIPRLGDGPDIARLREL 272 Query: 279 ANLRRPPRFICTTPSHQYPLGMVMSLSRRRMLLEYAATHKVWIIEDDYDSEFRYGG--RP 336 A + +P ++ + H P +S ++ +L A H I+EDD + G +P Sbjct: 273 AAVHKPKLYVINSVLHN-PTSTSLSAAKAFQVLRIAEEHDFVIVEDDIYCDMHPGSAVQP 331 Query: 337 LASLQGMDTQDRVLYMGTFSKIMFPGLRIGFLVVPESLARAFST-GIAELYRNGQVFLQA 395 + +D RV+Y+G FSK + LR+G++ LAR+ + + G++ + Sbjct: 332 ATRIAALDQLQRVIYLGGFSKTLSANLRVGYIATSAELARSLADRKLLSTLTTGEIG-ER 390 Query: 396 TLADFMAEGHFASHIRKMRVLYAERLQLLQQSINRHFGEKMTITGGEA---GLHLVLGLP 452 ++EGH+ H ++R RL + + R E++ + EA G+ L + Sbjct: 391 VAYKVLSEGHYRKHAERVRA----RLNGARDKVMRQL-ERLGMDVVEAPPCGMFLWVDTG 445 Query: 453 QECDDVLICEQALAAGIVVRPLSRY 477 ++ + ++ E A+ G ++ P S + Sbjct: 446 RDTN--VMTEAAMQEGFLLAPGSLF 468 >gi|300313928|ref|YP_003778020.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 472 Score = 127 bits (320), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 109/359 (30%), Positives = 170/359 (47%), Gaps = 23/359 (6%) Query: 62 LPAGMQLPSSRDLALELGSSRNTVTYAYEQLLAEGYLESRPGAGTFVADTAPDQIPEAV- 120 L +G +LPS R A +TV AYEQL+A G L++R GAG FVA A AV Sbjct: 38 LRSGERLPSVRQFAQHHDIGSSTVVEAYEQLVARGMLQARRGAGFFVAARAGQSGQHAVF 97 Query: 121 ERPEPLTDPSG-KSELSARGAMLTRQAGVGERQWGAFMPGVPDVTCVPYKIWGRLQNKHW 179 PEP+ D + SE+ A + +AG G ++PG + + + + + Sbjct: 98 VPPEPVIDSAWLLSEMFA-DERVPIKAGCG------WLPG----KWLNDEGLHQAERRII 146 Query: 180 RRSNADLLTYGPGAGYAGLREQIAEYLRVARSVNCTPSQVLITTGIHQSLDIVVKLLGEH 239 R A + YG GYA LR+ I ++L S+ QVL T G Q+LD+V++ + + Sbjct: 147 RSPGAQQVGYGHPYGYAPLRQLITQFLG-HWSLELGIEQVLTTHGATQALDLVIRTMTQP 205 Query: 240 GDSAWVEDPCYWGTRSV-----LNSLGIQSVPIAVDQEGMRMRLANLRRPPRFICTTPSH 294 GD+ ++DP Y ++ LN +G+ P VD + + LA P F TT Sbjct: 206 GDTVLIDDPGYCNLIAMLRLAGLNVIGVPRTPGGVDTDVLET-LARTHHPKMFF-TTSVL 263 Query: 295 QYPLGMVMSLSRRRMLLEYAATHKVWIIEDDYDSEFRYGGRPLASLQGMDTQDRVLYMGT 354 Q P G + + +L+ A H W++EDD E P+ L +D RV+Y+G+ Sbjct: 264 QNPTGTSYTPACAMRVLQAAERHGFWVVEDDIFRELGQATDPM--LAALDGLQRVIYVGS 321 Query: 355 FSKIMFPGLRIGFLVVPESLARAFSTGIAELYRNGQVFLQATLADFMAEGHFASHIRKM 413 +SK + P LR+G++ LAR L + + + + EGH H++ + Sbjct: 322 YSKTIAPSLRVGYIACQRELARQIVHTKMVLTLTNSEINERLVHNVLTEGHHRRHVQTL 380 >gi|300311139|ref|YP_003775231.1| MocR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 398 Score = 127 bits (319), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 91/363 (25%), Positives = 161/363 (44%), Gaps = 5/363 (1%) Query: 155 AFMPGVPDVTCVPYKIWGRLQNKHWRRSNADLLTYGPGAGYAGLREQIAEYLRVARSVNC 214 +F G+P P + ++ + L YGP GY LRE IA L Sbjct: 39 SFAGGLPSPLTFPVEHMKTAFDRVLSQQGKMALQYGPTDGYLPLREWIAASLST-NGAQI 97 Query: 215 TPSQVLITTGIHQSLDIVVKLLGEHGDSAWVEDPCYWGTRSVLNSLGIQSVPIAVDQEGM 274 + QVL+ +G Q LD++ K+L + G VE P Y G G + + D+ G+ Sbjct: 98 SAEQVLMVSGSQQGLDLLGKVLIDEGSKVLVETPSYLGALQAFALYGAKFESVPSDEFGL 157 Query: 275 RMR-LANLRRPPRFICTTPSHQYPLGMVMSLSRRRMLLEYAATHKVWIIEDDYDSEFRYG 333 + + + R + + P+ Q P G + RR L+E A + +IEDD Y Sbjct: 158 QPETIEAIAGGARMLYSLPNFQNPTGRTLPTERRFKLVETCARLGLPLIEDDPYGALSYQ 217 Query: 334 GRPLASLQGMDTQDRVLYMGTFSKIMFPGLRIGFLVVPESLARAFSTGIAELYRNGQVFL 393 PL + M+ V+YMG+FSK++ PG+R+G++V P L + Sbjct: 218 NAPLPKMLSMNPSG-VIYMGSFSKVLTPGIRLGYVVAPRPLILKMEQAKQATDLHTAQLT 276 Query: 394 QATLADFMAEGHFASHIRKMRVLYAERLQLLQQSINRHFGEKMTITGGEAGLHLVLGLPQ 453 Q + + + +G H+ +R LY ++ Q + ++ ++F + + E G+ + + LP+ Sbjct: 277 QMVVYEAIKDGFLDQHVPTIRKLYGDQCQAMLDALQQYFPASCSWSKPEGGMFIWVTLPE 336 Query: 454 ECD-DVLICEQALAAGIVVRPLSRYYMHARGARRGLLLGYASVPNDEIARAFDKLAAVIK 512 D L+ E + P + +Y + + L L + +VP ++I ++L +I Sbjct: 337 HIDAGALLNEAVEQEKVAFVPGAPFYANV-AQKNTLRLSFVTVPPEQIRAGVERLGKLIA 395 Query: 513 PHL 515 L Sbjct: 396 SKL 398 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,680,451 Number of extensions: 74461 Number of successful extensions: 265 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 246 Number of HSP's successfully gapped: 10 Length of query: 516 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 427 Effective length of database: 1,198,455 Effective search space: 511740285 Effective search space used: 511740285 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)