BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0470.p (333 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydro... 656 0.0 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 78 1e-15 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 72 5e-14 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 69 5e-13 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 56 5e-09 >gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 656 bits (1692), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/333 (98%), Positives = 330/333 (99%) Query: 1 MKAILTQPQSPLISAPDALIETILPDPTPGEHDLLVEVKAISVNPVDTKIRASAKEARVL 60 MKAILTQPQSPLISAPDALIET LPDPTPGEHDLLVEVKAISVNPVDTKIRASAKEARVL Sbjct: 1 MKAILTQPQSPLISAPDALIETTLPDPTPGEHDLLVEVKAISVNPVDTKIRASAKEARVL 60 Query: 61 GWDVSGVVRGVGPAVTLFAPGDEVFYAGSITRPGANSELHVVDERIVGRKPASLGHADAA 120 GWDVSGVVR VGPAVTLFAPGDEVFYAGSITRPGANSELHVVDERIVGRKP+SLGHADAA Sbjct: 61 GWDVSGVVRAVGPAVTLFAPGDEVFYAGSITRPGANSELHVVDERIVGRKPSSLGHADAA 120 Query: 121 AIPLTAITAWELLFDRVAVAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASR 180 AIPLTAITAWELLFDRVAVAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASR Sbjct: 121 AIPLTAITAWELLFDRVAVAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASR 180 Query: 181 PETAHWVGELGAHHVIDHSQPLKPQLEKLGIPEVGVVISLTHTDQHYAQIIDVLAPQGQL 240 PETAHWVGELGAHHVIDHSQPLKPQLEKLGIPEVG+VISLTHTDQHYAQIIDVLAPQGQL Sbjct: 181 PETAHWVGELGAHHVIDHSQPLKPQLEKLGIPEVGIVISLTHTDQHYAQIIDVLAPQGQL 240 Query: 241 ALIDDPASLDAMPLKRKSISLHWELMFTRSMFQTPDMIRQHELLQRVSQLLDQGVLRTTT 300 ALIDDPASLDAMPLKRKSISLHWELMFTRSMFQTPDMIRQHELLQRVSQLLDQGVLRTTT Sbjct: 241 ALIDDPASLDAMPLKRKSISLHWELMFTRSMFQTPDMIRQHELLQRVSQLLDQGVLRTTT 300 Query: 301 GEHFGRIDAANLRRAHALLESGKAKGKIVLEGF 333 GEH+GRIDAANLRRAH LLESGKAKGKIVLEGF Sbjct: 301 GEHYGRIDAANLRRAHGLLESGKAKGKIVLEGF 333 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 90/308 (29%), Positives = 135/308 (43%), Gaps = 17/308 (5%) Query: 29 PGEHDLLVEVKAISVNPVDTKIRASAKEARV---LGWDVSGVVRGVGPAVTLFAPGDEVF 85 PG ++ + A+ +N +D RA + LG + +G + VG V+ F GD V Sbjct: 26 PGAGEVRIRHAAVGLNFIDCYFRAGLYPQPLPAGLGQEAAGTIEAVGAGVSGFKVGDRVA 85 Query: 86 YAGSITRP-GANSELHVVDERIVGRKPASLGHADAAAIPLTAITAWELLFDRVAVAEGGG 144 YAG RP GA +E V+ I+ + P ++ AAA+ L +T LL AV Sbjct: 86 YAG---RPNGAYAEERVMPADILVKLPDAISFDTAAAMMLQGMTVQYLLRQTYAVQP--- 139 Query: 145 QGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASRPETAHWVGELGAHHVIDH-SQPLK 203 G +L AAGG+G + Q AR L + +IGT S E A + GA HVI++ ++ Sbjct: 140 -GDTILFHAAAGGIGLIACQWARALG-VNLIGTVSSDEKAALALQHGAAHVINYKTENFV 197 Query: 204 PQLEKL-GIPEVGVVISLTHTDQHYAQIIDVLAPQGQLALIDDPASLDAMPLKRKSISLH 262 ++++L G V VV D +D L P+G + + AS P ++ Sbjct: 198 ERVKELTGGRGVQVVYDSIGKDTFLGS-LDCLRPRGMMVSFGN-ASGPVAPFGVSELASR 255 Query: 263 WELMFTRSMFQTPDMIRQHELLQRVSQLLDQGVLRTTTGEHFGRIDAANLRRAHALLESG 322 L TR R EL L D + + E R ++ +AHA LE Sbjct: 256 GSLFLTRPSLGNYTSTRA-ELDATAGDLFDMVLGNKISIEINQRYALKDVAQAHADLEGR 314 Query: 323 KAKGKIVL 330 K G +L Sbjct: 315 KTTGSTIL 322 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 72.4 bits (176), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 94/334 (28%), Positives = 140/334 (41%), Gaps = 51/334 (15%) Query: 24 LPDPTPGEHDLLVEVKAISVNPVDTKIRASAKE-----------ARVLGWDVSGVVRGVG 72 +P+ PGE +LV V A+ +NP D +R K +LG D+SGVV V Sbjct: 27 VPELQPGE--VLVRVHAVGLNPPDWYLREGYKMLPPEWQPTVAFPLILGTDISGVVEAVA 84 Query: 73 PAVTLFAPGDEV-----FYAGSITRPGANSELHVVDERIVGRKPASLGHADAAAIPLTAI 127 V FA GDEV F +G A ++ V + KP + HA AAA P++ + Sbjct: 85 DDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEIALKPQRISHAQAAAAPMSLL 144 Query: 128 TAWELLFDRVAVAEGGGQ----------GQHLLIIGAAGGVGSMLTQLAR-KLTQLTVIG 176 TAW+ + + + Q + +++ GAAGGVG QLA+ K ++ IG Sbjct: 145 TAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGGVGHFALQLAKWKGARVIAIG 204 Query: 177 TASRPETAHWVGELGAHHVIDHSQPLKPQLEKLGIPEVGVVISLTHTDQHYAQIIDVLAP 236 + ++ +LGA ID+++ + + +V +VI + + L Sbjct: 205 SGGHEA---FLRDLGADEFIDYTRSAAEDV----LSDVDLVIDAVGGPAS-GRFLRTLKR 256 Query: 237 QGQLALIDDPASLDAMPLKRKSISLHWELMFTRSMFQTPDMIRQHELLQRVSQLLDQGVL 296 G L I PL L S Q Q LQ+++ LLD GV+ Sbjct: 257 GGALFPI--------FPLGFSGAEEAARLGVNVSTTQVRSSGAQ---LQQLAGLLDSGVV 305 Query: 297 RTTTGEHFGRIDAANLRRAHALLESGKAKGKIVL 330 R F DA +AH G +GKIVL Sbjct: 306 RVAIDSTFALQDAG---KAHERAAQGHIRGKIVL 336 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 68.9 bits (167), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 89/315 (28%), Positives = 143/315 (45%), Gaps = 41/315 (13%) Query: 27 PTPGEHDLLVEVKAISVNPVDTKIRASAKE-------ARVLGWDVSGVVRGVGPAVTLFA 79 P P H + V ++A V PVDTK+RA + +V G D +GV+ +G V Sbjct: 24 PLPPGH-VRVAMQAAGVAPVDTKLRAGRLQHLVALDLPKVPGRDGAGVITALGEGVHTLQ 82 Query: 80 PGDEVFYAGSITRPGAN-SELHVVDERIVGRKPASLGHADAAAIPLTAITAWELLFDRVA 138 G+ V S+ G +EL + E++V RKP L AAA+ A +AW L Sbjct: 83 VGEAVCVMPSMAGRGTTLAELVLPQEQVV-RKPDVLSMQQAAALLQPACSAWIALMQTAP 141 Query: 139 VAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASRPETAHWVGELGAHHVIDH 198 +A G + I +G VGS++ QLA L + + R +V LGA V+ + Sbjct: 142 IAA----GMKVFIHAGSGAVGSLMVQLAAHLG--AEVTASCRSSNVEYVAALGASRVLAY 195 Query: 199 SQPLKPQLEKLG-IPEVGVVISLTHTDQHYAQIIDVLAPQGQLALIDDPASLDAMPLKRK 257 + ++LG + + VVI L H Q +L GQL L A P++ + Sbjct: 196 DR------DELGRVRDQDVVIDLVGGAAH-DQSYRMLRRGGQLVW------LVAAPIRER 242 Query: 258 SISLHWELMFTRSMFQTPDMIRQHELLQRVSQLLDQGVLRTTTGEHFGRIDAANLRRAHA 317 + + R++ ++ +LQ+V+ L QGV R G + + RAH Sbjct: 243 --GEEFGVSVRRAL-----IVEDQSILQQVAALAQQGVWRPAVS---GVLPMQEIARAHQ 292 Query: 318 LLESGK-AKGKIVLE 331 +E+G+ +G++VL+ Sbjct: 293 RIEAGEVTRGRLVLD 307 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 55.8 bits (133), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 83/331 (25%), Positives = 140/331 (42%), Gaps = 27/331 (8%) Query: 13 ISAPDALIETILPDPTPGEHDLLVEVKAISVNPVDTKIRASAK-----EARVLGWDVSGV 67 P+ L P P ++L++V A VN D R + + G +V+G Sbjct: 9 FGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDLPGLEVAGE 68 Query: 68 VRGVGPAVTLFAPGDEVFYAGSITRPGANSELHVVDERIVGRKPASLGHADAAAIPLTAI 127 + + F GD V ++ + G +E P L +AAA+P Sbjct: 69 IVAGDLGDSGFKLGDLVC---ALVQGGGYAEFCAAPLAQCLPVPKGLSALEAAALPENYF 125 Query: 128 TAWELLFDRVAVAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASRPETAHWV 187 T W +FDR ++ G+ + LL+ G + G+G Q+AR L V TA E Sbjct: 126 TVWSNVFDRGQLS---GE-ETLLVQGGSSGIGVTAIQIARALGH-RVFATAGSAEKCRAC 180 Query: 188 GELGAHHVIDH-SQPLKPQLEKL-GIPEVGVVISLTHTDQHYAQIIDVLAPQGQLALIDD 245 ELGA I++ ++ + +++L G V V++ + D + + I LA G+L I Sbjct: 181 EELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGD-YLPREIKALADDGRLVFIAQ 239 Query: 246 ----PASLDAMPLKRKSISLHWELMFTRSM-FQTPDMIRQHELLQRVSQLLDQGVLRTTT 300 LD + R+ +++ + R + F+ + L + V L++ G +R Sbjct: 240 LGGAKGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIAAK---LREHVWPLIEAGKIRPVI 296 Query: 301 GEHFGRIDAANLRRAHALLESGKAKGKIVLE 331 F AA +AHA++E GKI+LE Sbjct: 297 HHRFPLEQAA---QAHAMMEGSTHIGKIMLE 324 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,099,600 Number of extensions: 47386 Number of successful extensions: 196 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 5 Length of query: 333 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 247 Effective length of database: 1,212,660 Effective search space: 299527020 Effective search space used: 299527020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)