BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0469.p (440 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 892 0.0 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 97 3e-21 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 94 3e-20 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 78 1e-15 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 892 bits (2306), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/440 (99%), Positives = 439/440 (99%) Query: 1 LENILLIVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYL 60 +ENILLIVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYL Sbjct: 1 MENILLIVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYL 60 Query: 61 SACQLGITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVA 120 SACQLGITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVA Sbjct: 61 SACQLGITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVA 120 Query: 121 GELAPKSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAH 180 GELAPKSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAH Sbjct: 121 GELAPKSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAH 180 Query: 181 YSSDELKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSL 240 YSSDELKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSL Sbjct: 181 YSSDELKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSL 240 Query: 241 EENLDIIYRNRYSRYPYYDAERQQVLGLVHLKDVFLAQQDGRAIANLKDYLRPVQYISPA 300 EENLDIIYRNRYSRYPYYDAERQQVLGLVHLKDVFLAQQDGRAIANLKDYLRPVQYISPA Sbjct: 241 EENLDIIYRNRYSRYPYYDAERQQVLGLVHLKDVFLAQQDGRAIANLKDYLRPVQYISPA 300 Query: 301 LPALDLLRRFRTGSPHFAIIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDD 360 LPALDLLRRFRTGSPHFA+IGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDD Sbjct: 301 LPALDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDD 360 Query: 361 GTLLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKR 420 GTLLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKR Sbjct: 361 GTLLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKR 420 Query: 421 MSGPKIVLVKVYPKLQEVRE 440 MSGPKIVLVKVYPKLQE RE Sbjct: 421 MSGPKIVLVKVYPKLQEARE 440 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 96.7 bits (239), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 92/433 (21%), Positives = 192/433 (44%), Gaps = 31/433 (7%) Query: 7 IVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAV-VHNQLDAYLSACQL 65 ++ F L+ L+ FF E +L+ + R+R +AK QG R I + + ++ + LS + Sbjct: 10 VLALFILILLSAFFSMTETALMAANRHRLRHLAK-QGNRAAITILWLLDRTERLLSLVLI 68 Query: 66 GITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELAP 125 T + A A L ++A N H A ++ L I+ E+ P Sbjct: 69 ANTAIN---------ALATALVTALAIAAFGN----HQRVITAATACVAALLIIFAEIVP 115 Query: 126 KSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAG-HGHDAHYSSD 184 K + PE++ L+ L PLIW +N + +LR+ + G + S + Sbjct: 116 KVIGATYPERISLFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPE 175 Query: 185 ELKLILRAGNKSGKNGKFT-RDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEEN 243 EL+ I+ G G F + ++L + ++ V D+M P S++ AL +++ Sbjct: 176 ELRSIVLEG------GSFIPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDI 229 Query: 244 LDIIYRNRYSRYPYYDAERQQVLGLVHLKDV--FLAQQDGRAIANLKDYLRPVQYISPAL 301 L + +++ P ++ E ++G++H++ +++ + ++++ L Y+ Sbjct: 230 LHQLATCYHNKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVET 289 Query: 302 PALDLLRRFRTGSPHFAIIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGE---WTRQ 358 L+ F+ ++ + G +TL++++ ++GE ++GE W + Sbjct: 290 DVFTQLQYFQENHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDK- 348 Query: 359 DDGTLLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVI 418 DG L +GS + + LG+ + D ++ GL++E L D+P+ + + I Sbjct: 349 -DGACLLEGSTTLRDINKRLGLQLPLDGP-KTINGLLLEYLQDIPEASVAVKMDGCVIEI 406 Query: 419 KRMSGPKIVLVKV 431 ++ I +VK+ Sbjct: 407 VQVQNQAIRMVKL 419 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 93.6 bits (231), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 93/416 (22%), Positives = 181/416 (43%), Gaps = 18/416 (4%) Query: 17 NGFFVAAEFSLVKLRQTRIRAIAKTQGMRG--RILAVVHNQLDAYLSACQLGITLASLGL 74 + F AE S+ R+ R+ ++K +G+ R+L++ Q + + Q+G+ ++ Sbjct: 17 SAFLSCAEISIAASRKIRLEMMSK-EGVHNADRVLSL-QAQPGNFFTVVQIGLNAVAILG 74 Query: 75 GWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELAPKSMAIRSPE 134 G +GEPA + + S + + SF +F V++ I+ +L PK +A+ +PE Sbjct: 75 GVVGEPALTPHMREIISSFYIG--PWLENASFAMSFLVVTAFFILFADLMPKRLAMVAPE 132 Query: 135 KLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAHYSSDELKLILRAGN 194 + + P+ PL+W N+ S W+ + GL + +E+ ++ AG Sbjct: 133 RAAVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGL--PQVRQEVITPNEIHAMVDAGA 190 Query: 195 KSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENLDIIYRNRYSR 254 ++ G E +++ V M P IV S I + +SR Sbjct: 191 QA---GVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPHSR 247 Query: 255 YPYYDAERQQVLGLVHLKDVF--LAQQDGRAIANLKDYLRPVQYISPALPALDLLRRFRT 312 + D VLG + KDV L + + ++A+ K + P I +L ++L R + Sbjct: 248 FLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLNLWEVLERMKA 306 Query: 313 GSPHFAIIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDDGTLLGKGSLPIV 372 AI+ + G I+ ++ +L+G + + R+D+ + L G P+ Sbjct: 307 MDEDLAIVVNEYALVVGIISTTDVTGVLMGNLL-AITEEEQQIVRRDENSWLMDGLTPLN 365 Query: 373 TLENMLGIDIEYDDSI--DSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKRMSGPKI 426 + + L ID EY + + +++ G +M L +PK+ + F F + + G +I Sbjct: 366 DVMSELDID-EYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 63/238 (26%), Positives = 116/238 (48%), Gaps = 13/238 (5%) Query: 205 DEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENLDIIYRNRYSRYPYYDAERQQ 264 D +++ ++L V DIM P S++ + K +E + + +SR+P D ER + Sbjct: 52 DALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDK 111 Query: 265 VLGLVHLKDV--FLAQQDGRAIANLKDYLRPVQYISPALPALDLLRRFRTGSPHFAIIGK 322 V+G++ KD+ + A++ +++D LRP +I + LLR FR H A++ Sbjct: 112 VVGILLAKDLLRYYAEES----FDVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVD 167 Query: 323 KGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWT--RQDDGTLLGKGSLPIVTLENMLGI 380 + AG IT++++L +VG+I DE+ + E DG + + +T Sbjct: 168 EYGGVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKALTEIEQFNE 227 Query: 381 DIE---YDDSIDSVGGLVMEKLGDLPKEGQKIDFPA--FDVVIKRMSGPKIVLVKVYP 433 ++ DD D++GGLV +G +P++G + D F+V+ ++LV P Sbjct: 228 EVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQLP 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.142 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,314,758 Number of extensions: 56522 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 4 Length of query: 440 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 352 Effective length of database: 1,203,190 Effective search space: 423522880 Effective search space used: 423522880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)