BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0467.p (597 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310421|ref|YP_003774513.1| hypothetical protein Hsero_1088... 939 0.0 gi|300312345|ref|YP_003776437.1| cell wall surface anchor protei... 380 e-106 gi|300313785|ref|YP_003777877.1| autotransporter adhesin protein... 195 8e-51 gi|300312343|ref|YP_003776435.1| hypothetical protein Hsero_3039... 77 5e-15 >gi|300310421|ref|YP_003774513.1| hypothetical protein Hsero_1088 [Herbaspirillum seropedicae SmR1] Length = 8016 Score = 939 bits (2428), Expect = 0.0, Method: Compositional matrix adjust. Identities = 483/520 (92%), Positives = 492/520 (94%), Gaps = 9/520 (1%) Query: 1 MTTTVLKLSESSAFLKTISLKSIKDVRALDVDLLLVLTNGDQILISGGAMHALNSPDMAL 60 MTTTVLKLSESSAFLKTISLKSIKDVRALDVDLLLVLTNGDQILISGGAMHALNSPDMAL Sbjct: 1 MTTTVLKLSESSAFLKTISLKSIKDVRALDVDLLLVLTNGDQILISGGAMHALNSPDMAL 60 Query: 61 QFSDGQLPLARVFEQIERINVSPEANLTVSSKEITRYNQNNARKKKARQQDEEGDKPIIS 120 QFSDGQLPLARVFEQIERINVSPEANLTVSSKEITRYNQNNARKKKARQQDEEGDKPIIS Sbjct: 61 QFSDGQLPLARVFEQIERINVSPEANLTVSSKEITRYNQNNARKKKARQQDEEGDKPIIS 120 Query: 121 PDDEKDPVAATETNGTGGNTNSTDYSPIRSTDNRKQLAEAEISSEKEKSWGVQWPIAAGV 180 PDDEKDPVAATETNGTGGNTNSTDYSPIRSTDNRKQLAEAEISSEKEKSWGVQWPIAAGV Sbjct: 121 PDDEKDPVAATETNGTGGNTNSTDYSPIRSTDNRKQLAEAEISSEKEKSWGVQWPIAAGV 180 Query: 181 LAVLAAAGGGGGGGGAGAAGASTAGGGGSGAASVEPPKANLEGAVVLGAIRNATITAYDN 240 LAVLAAAGGGGGGGGAGAAGASTAGGGGSGAASVEPPKANLEGAVVLGAIRNATITAYDN Sbjct: 181 LAVLAAAGGGGGGGGAGAAGASTAGGGGSGAASVEPPKANLEGAVVLGAIRNATITAYDN 240 Query: 241 AGNALSAATEVVDGKYALVLTRPFYKGPMLLVVRDNTPEVADNYIDEATQTITNLGATPL 300 AGNALSAATEVVDGKYALVLTRPFYKGPMLLVVRDNTPEVADNYIDEATQTITNLGATPL Sbjct: 241 AGNALSAATEVVDGKYALVLTRPFYKGPMLLVVRDNTPEVADNYIDEATQTITNLGATPL 300 Query: 301 RALVEANGVNQTVNVTALTELAVQKAGLAAGVTTLAGAPEVTTERINSANAAVGAFFKVD 360 RALVEANGVNQTVNVTALTELAVQKAGLAAGVTTLAGAPEVTTERINSANAAVGAFFKVD Sbjct: 301 RALVEANGVNQTVNVTALTELAVQKAGLAAGVTTLAGAPEVTTERINSANAAVGAFFKVD 360 Query: 361 AIAGEVVPTHIANSQGVGIVNPAYNAAISAGAQNYGAALKALANLVLLDDRTYPNQAAVL 420 AIAGEVVPTHIANSQGVG+VNPAYNAAISAGAQNYGAALKALANLVLLDDRTYPNQAAVL Sbjct: 361 AIAGEVVPTHIANSQGVGVVNPAYNAAISAGAQNYGAALKALANLVLLDDRTYPNQAAVL 420 Query: 421 QKLAEALVFVDEARSALKWATTAQGEPMAAAAAIQAALFSEALQQTMADLNNSERDHITA 480 QKLAEALVFVDEARSALKWATTAQGEP+AAAAAIQAALFSEALQQTMADLNNSE T Sbjct: 421 QKLAEALVFVDEARSALKWATTAQGEPLAAAAAIQAALFSEALQQTMADLNNSE----TT 476 Query: 481 RHECIQKGRRPAHQARPTGHRLQPAQIR-HCAWDLAQPYL 519 R + K + Q PTG + ++ H A +A P + Sbjct: 477 RAQASVKLAK--LQKLPTGTTVADYLVKNHVA--IANPVI 512 >gi|300312345|ref|YP_003776437.1| cell wall surface anchor protein [Herbaspirillum seropedicae SmR1] Length = 7175 Score = 380 bits (976), Expect = e-106, Method: Compositional matrix adjust. Identities = 230/420 (54%), Positives = 290/420 (69%), Gaps = 6/420 (1%) Query: 50 MHALNSPDMALQFSDGQLPLARVFEQIERINVSPEANLTVSSKEITRYNQNNARKKKARQ 109 M ALN+P+ LQF+DGQL + R+F+QI++INVSPEANLT+SSKEITRYNQN + KA++ Sbjct: 1 MQALNAPETPLQFADGQLQMGRMFQQIDQINVSPEANLTISSKEITRYNQNTIKASKAKR 60 Query: 110 QDEEGDKPIISPDDEKDPVAATETNGTGGNTNSTDYSPIRSTDNRKQLAEAEISSEKEKS 169 +++ KP+++ EK P A G GN + SP+++ D +KQ+A+ EI+S EK+ Sbjct: 61 TEDDSQKPVVAEQGEKAPEVADPNTGVPGNIAQPESSPVKTPDMQKQMADVEINSRSEKN 120 Query: 170 WGVQWPIAAGVLAVLAAAGGGGGGGGAGAAGASTAGGGGSGAASVEPPKANLEGAVVLGA 229 WGVQWPIAAGVLA+LAAA GG G GA A S A + E A + GA +LG Sbjct: 121 WGVQWPIAAGVLALLAAA-----AGGGGGGGAGAAAAAASPADTPEKINAVISGAAMLGP 175 Query: 230 IRNATITAYDNAGNALSAATEVVDGKYALVLTRPFYKGPMLLVVRDNTPEVADNYIDEAT 289 + NAT TAYD+ GNA+S A +V+DG Y+L L P YKG +L+V+RDNTP V DN+ DEA+ Sbjct: 176 LNNATATAYDSRGNAISDAVQVIDGHYSLTLKSPGYKGVLLVVIRDNTPGVPDNFGDEAS 235 Query: 290 QTITNLGATPLRALVEANGVNQTVNVTALTELAVQKAGLAAGVTTLAGAPEVTTERINSA 349 +T+LG PLRAL A GVNQTVNVTALTELA KAGLA G GA + +N+A Sbjct: 236 LRMTDLGVIPLRALAVAGGVNQTVNVTALTELAAIKAGLAQGQVN-PGAASLDVNAVNNA 294 Query: 350 NAAVGAFFKVDAIAGEVVPTHIANSQGVGIVNPAYNAAISAGAQNYGAALKALANLVLLD 409 N AVG FKVDAIAGEVVPT N QG I N A+ AIS A NYGAALKA+ANLV LD Sbjct: 295 NNAVGGLFKVDAIAGEVVPTSTVNVQGQVISNAAFTNAISNQAHNYGAALKAIANLVELD 354 Query: 410 DRTYPNQAAVLQKLAEALVFVDEARSALKWATTAQGEPMAAAAAIQAALFSEALQQTMAD 469 YP QA +QKLA+AL FVD A ++LKWAT +G+ + AA +Q+ LFSE LQ+ + D Sbjct: 355 RNNYPTQADAIQKLADALQFVDGAGASLKWATNEKGQALPAANLMQSHLFSEPLQKIIYD 414 >gi|300313785|ref|YP_003777877.1| autotransporter adhesin protein [Herbaspirillum seropedicae SmR1] Length = 3631 Score = 195 bits (496), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 120/271 (44%), Positives = 168/271 (61%), Gaps = 12/271 (4%) Query: 219 ANLEGAVVLGAIRNATITAYDNAGNALSAATEVVDGKYALVLTRPFYKGPMLLVVRDNTP 278 + L GA LG + NAT+TAYD+ G + V++G+Y+LVL P YKG +LL VRD+TP Sbjct: 275 STLGGATSLGPLTNATVTAYDSQGRMIGKPVPVINGRYSLVLDVPGYKGLILLAVRDSTP 334 Query: 279 EVADNYIDEATQTITNLGATPLRALVEANGVNQTVNVTALTELAVQKAGLAAGVTTLAGA 338 VADN+ DEAT I +LG T LR++V+A G NQT+N+TALTELA KAGLA+G T L A Sbjct: 335 GVADNFADEATLRIADLGDTVLRSVVQAEGGNQTINITALTELAALKAGLASGQTNLGAA 394 Query: 339 PEVTTERINSANAAVGAFFKVDAIAGEVVPTHIANSQGVGIVNPAYNAAISAGAQNYGAA 398 +VT + +ANAAV A F V+ +G+V+ T + ++ G +NPA+ + + A++YG A Sbjct: 395 SQVTAPTVAAANAAVSALFMVNISSGDVLTTTVTDANGAAAINPAFARSSNTSARHYGIA 454 Query: 399 LKALANLVLLDDRTYPNQAAVLQKLAEALVFVDEARSALKWATTAQGEPMAAAAAIQAAL 458 LKA+ANL L D YP+Q +QKLA++L F D + LKW T Q+ L Sbjct: 455 LKAIANLQLTDATRYPDQGVAIQKLADSLGFSDTQPNKLKWRDT----------VTQSDL 504 Query: 459 FSEALQQTMAD--LNNSERDHITARHECIQK 487 F+E + D L+ +R A + +QK Sbjct: 505 FNERIASRANDSSLSAEQRQQARALLDVLQK 535 >gi|300312343|ref|YP_003776435.1| hypothetical protein Hsero_3039 [Herbaspirillum seropedicae SmR1] Length = 132 Score = 76.6 bits (187), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 38/80 (47%), Positives = 54/80 (67%) Query: 2 TTTVLKLSESSAFLKTISLKSIKDVRALDVDLLLVLTNGDQILISGGAMHALNSPDMALQ 61 TT L ++++ L+ I ++SIKDVR+L +DLLLV ++G QIL+ GA+ AL P LQ Sbjct: 16 TTYTLDIAQAGTALRKIPIESIKDVRSLGIDLLLVFSSGGQILLREGALEALVHPQRRLQ 75 Query: 62 FSDGQLPLARVFEQIERINV 81 FSDG L L ++F Q ER + Sbjct: 76 FSDGVLTLQQIFTQSERFDF 95 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.128 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,497,470 Number of extensions: 56043 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 4 Length of query: 597 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 506 Effective length of database: 1,188,985 Effective search space: 601626410 Effective search space used: 601626410 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)