BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0466.p (585 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310422|ref|YP_003774514.1| gamma-glutamyltranspeptidase [H... 1200 0.0 gi|300313075|ref|YP_003777167.1| gamma-glutamyltranspeptidase [H... 143 3e-35 >gi|300310422|ref|YP_003774514.1| gamma-glutamyltranspeptidase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 1200 bits (3105), Expect = 0.0, Method: Compositional matrix adjust. Identities = 585/585 (100%), Positives = 585/585 (100%) Query: 1 MQTFTGLCKPALALLPLCGALAFAPAQAQTAAPAAPVVKYDLTYDIISPVHSQHGMVATE 60 MQTFTGLCKPALALLPLCGALAFAPAQAQTAAPAAPVVKYDLTYDIISPVHSQHGMVATE Sbjct: 1 MQTFTGLCKPALALLPLCGALAFAPAQAQTAAPAAPVVKYDLTYDIISPVHSQHGMVATE 60 Query: 61 QELATKVGVDILKKGGNAVDAGVAVGFALAVVLPNAGNIGGGGFMMIHDAKTGKNVALDF 120 QELATKVGVDILKKGGNAVDAGVAVGFALAVVLPNAGNIGGGGFMMIHDAKTGKNVALDF Sbjct: 61 QELATKVGVDILKKGGNAVDAGVAVGFALAVVLPNAGNIGGGGFMMIHDAKTGKNVALDF 120 Query: 121 REMAPQRATRNMYLDDKGNVVPGRSLYTHLAVGVPGTVAGLSHALSKYGTMKLSQVIQPA 180 REMAPQRATRNMYLDDKGNVVPGRSLYTHLAVGVPGTVAGLSHALSKYGTMKLSQVIQPA Sbjct: 121 REMAPQRATRNMYLDDKGNVVPGRSLYTHLAVGVPGTVAGLSHALSKYGTMKLSQVIQPA 180 Query: 181 VELAEKGYPVSRSLAQILSAEREHLGQWDSSKAIFFKEGRPLQEGELLVQKDLAKSLRLI 240 VELAEKGYPVSRSLAQILSAEREHLGQWDSSKAIFFKEGRPLQEGELLVQKDLAKSLRLI Sbjct: 181 VELAEKGYPVSRSLAQILSAEREHLGQWDSSKAIFFKEGRPLQEGELLVQKDLAKSLRLI 240 Query: 241 AKEGPKAFYEGSIAKKIVAEMDKHGGLISADDLKNYKVVERVPVSGNYRGYQVMSMPPPS 300 AKEGPKAFYEGSIAKKIVAEMDKHGGLISADDLKNYKVVERVPVSGNYRGYQVMSMPPPS Sbjct: 241 AKEGPKAFYEGSIAKKIVAEMDKHGGLISADDLKNYKVVERVPVSGNYRGYQVMSMPPPS 300 Query: 301 SGGVHIIQMMNILERYPLKQYGADSAQTIHLMAETMKLAYADRSEYLGDPDFTKVPVKGL 360 SGGVHIIQMMNILERYPLKQYGADSAQTIHLMAETMKLAYADRSEYLGDPDFTKVPVKGL Sbjct: 301 SGGVHIIQMMNILERYPLKQYGADSAQTIHLMAETMKLAYADRSEYLGDPDFTKVPVKGL 360 Query: 361 TSRAYADELAKKINPDRATPSSEIKPGQPQPYESDQTTHYSVADKDGNLVATTYTLNLNF 420 TSRAYADELAKKINPDRATPSSEIKPGQPQPYESDQTTHYSVADKDGNLVATTYTLNLNF Sbjct: 361 TSRAYADELAKKINPDRATPSSEIKPGQPQPYESDQTTHYSVADKDGNLVATTYTLNLNF 420 Query: 421 GSGIVATGTGITLNNEMDDFAAKPGVPNAFGLVGGDANAVAPYKRPLSSMSPTFVLKDGK 480 GSGIVATGTGITLNNEMDDFAAKPGVPNAFGLVGGDANAVAPYKRPLSSMSPTFVLKDGK Sbjct: 421 GSGIVATGTGITLNNEMDDFAAKPGVPNAFGLVGGDANAVAPYKRPLSSMSPTFVLKDGK 480 Query: 481 PFLVTGSPGGSRIITTTLQTILNVIDHDMNVAEATITPRIHHQWAPDQLRVEKGLSADTL 540 PFLVTGSPGGSRIITTTLQTILNVIDHDMNVAEATITPRIHHQWAPDQLRVEKGLSADTL Sbjct: 481 PFLVTGSPGGSRIITTTLQTILNVIDHDMNVAEATITPRIHHQWAPDQLRVEKGLSADTL 540 Query: 541 KILQDKGQKISVQPSMGRTQTIQIRSDGFDGFSDPRNPDGRTLGF 585 KILQDKGQKISVQPSMGRTQTIQIRSDGFDGFSDPRNPDGRTLGF Sbjct: 541 KILQDKGQKISVQPSMGRTQTIQIRSDGFDGFSDPRNPDGRTLGF 585 >gi|300313075|ref|YP_003777167.1| gamma-glutamyltranspeptidase [Herbaspirillum seropedicae SmR1] Length = 527 Score = 143 bits (361), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 147/542 (27%), Positives = 232/542 (42%), Gaps = 35/542 (6%) Query: 55 GMVATEQELATKVGVDILKKGGNAVDAGVAVGFALAVVLPNAGNIGGGGFMMIHDAKTGK 114 GMV+ LA + G IL++GGNAV+A VA +AVV P+ IGG GF +I + Sbjct: 10 GMVSAPHHLAAQAGAQILRQGGNAVEAMVAAAATIAVVYPHMNGIGGDGFWLISEPGRAP 69 Query: 115 NVALDFREMAPQRATRNMYLDDKGNVVPGRSLYTHL--AVGVPGTVAGLSHALSKYGTMK 172 VA+ A A + Y + +P R + L A V G A L HA + Sbjct: 70 -VAIRACGPAAALANKAFYAERGYRAMPTRGPHAALTVAGAVAGWAAALEHAQRFASGLP 128 Query: 173 LSQVIQPAVELAEKGYPVSRSLAQILSAEREHLGQWDSSKAIFFKEGRPLQEGELLVQKD 232 L ++ A+ A+ G PV+RS ++ + L A + +G P Q G++L Q Sbjct: 129 LPVLLADAIGHAQAGIPVTRSQHELTRDKWSELKDQPGFAATYAADGVPPQ-GKVLKQSA 187 Query: 233 LAKSLRLIAKEGPKAFYEGSIAKKIVAEMDKHGGLISADDLKNYKVVERVPVSGNYRGYQ 292 LA SL +A+ G FY G +A+ + A + G + DL+ ++ P+ + Sbjct: 188 LAASLSRLAEAGLDDFYRGDLAQSLAAGLQAAGSPLRRSDLEAFRAQVVQPLMVQLSSGR 247 Query: 293 VMSMPPPSSGGVHIIQMMNILERYPLKQYGADSAQTIHLMAETMKLAYADRSEYLGDPDF 352 V ++PPP+ GV + ++ + +R + Q AD +H + E K A+ R++++ DP Sbjct: 248 VYNLPPPTQ-GVSALMIIGLFDRLGVTQ--ADGFAHLHGLVEATKRAFILRNQHVTDPAH 304 Query: 353 TKVPVKGLTSRAYADELAKKINPDRATPSSEIKPGQPQPYESDQTTHYSVADKDGNLVAT 412 +V D+ + I+ RA P P P T AD G +V+ Sbjct: 305 MRVDAAQWLLAGALDQEVRHIDMQRAAP-------WPHPARPGDTIWMGAADAQGRVVSY 357 Query: 413 TYTLNLNFGSGIVATGTGITLNNEMDDFAAKPGVPNAFGLVGGDANAVAPYKRPLSSMSP 472 ++ FGSG+V TGI N F GV N + P K P +++P Sbjct: 358 IQSIFWEFGSGVVVPDTGIVWQNRGASFTLDEGV-----------NQLQPGKLPFHTLNP 406 Query: 473 TFVLKDGKPFLVTGSPGGSRIITTTLQTILNVIDHDMNVAEATITPR--IHHQWA--PDQ 528 + D L G+ GG T + N+ A PR + W Sbjct: 407 SLAQLDDGRTLAFGTMGGEGQPQTQAAVFTRHVLFGQNLQAAVSAPRWLLGRTWGDVSTN 466 Query: 529 LRVEKGLSADTLKILQDKGQKISV----QPSMGRTQTIQIRSDGF-DGFSDPRNPDGRTL 583 L++E + T++ L+ G ++ V MG + + DG +G +DPR DG Sbjct: 467 LKLEGRIPEATIQALRRAGHEVQVVDDYADMMGHAGAVCVHPDGLIEGATDPRA-DGVCA 525 Query: 584 GF 585 G Sbjct: 526 GL 527 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,729,458 Number of extensions: 72002 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 2 Length of query: 585 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 495 Effective length of database: 1,193,720 Effective search space: 590891400 Effective search space used: 590891400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)