BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0463.p (450 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310424|ref|YP_003774516.1| high-affinity gluconate permeas... 867 0.0 gi|300311081|ref|YP_003775173.1| high-affinity gluconate transpo... 481 e-137 gi|300311416|ref|YP_003775508.1| gluconate transporter family pr... 327 1e-90 >gi|300310424|ref|YP_003774516.1| high-affinity gluconate permease [Herbaspirillum seropedicae SmR1] Length = 450 Score = 867 bits (2240), Expect = 0.0, Method: Compositional matrix adjust. Identities = 449/450 (99%), Positives = 449/450 (99%) Query: 1 MLGMSHDATLLFNAVIAILALILMITRLRIHPFIALTITSGLLGLISGMPLPKIVKSFQD 60 MLGMSHDATLLFNAVIAILALILMITRLRIHPFIALTITSGLLGLISGMPLPKIVKSFQD Sbjct: 1 MLGMSHDATLLFNAVIAILALILMITRLRIHPFIALTITSGLLGLISGMPLPKIVKSFQD 60 Query: 61 GFGGVLGFVGIVLGLGTMLGKLMAESGGADQIAQTLVRAFGRERVHWAMMMGAFLVGIPL 120 GFGGVLGFVGIVLGLGTMLGKLMAESGGADQIAQTLVRAFGRERVHWAMMMGAFLVGIPL Sbjct: 61 GFGGVLGFVGIVLGLGTMLGKLMAESGGADQIAQTLVRAFGRERVHWAMMMGAFLVGIPL 120 Query: 121 FFEIGFVLLIPLVFIVARRSGVPLFKIGMPMLASLSVMHGLVPPHPGPLLAIGIFGADIG 180 FFEIGFVLLIPLVFIVARRSGVPLFKIGMPMLASLSVMHGLVPPHPGPLLAIGIFGADIG Sbjct: 121 FFEIGFVLLIPLVFIVARRSGVPLFKIGMPMLASLSVMHGLVPPHPGPLLAIGIFGADIG 180 Query: 181 KTIFYGLLVGLPTVIIAGPMFGAFISRYVDIKPSEELMAQLAREPQTTKLPGFATTLITV 240 KTIFYGLLVGLPTVIIAGPMFGAFISRYVDIKPSEELMAQL REPQTTKLPGFATTLITV Sbjct: 181 KTIFYGLLVGLPTVIIAGPMFGAFISRYVDIKPSEELMAQLVREPQTTKLPGFATTLITV 240 Query: 241 LAPVFLMLLKTLVDVTLPEGHAMRDLMDFIGNPIVALLAALLLAIWTFGIARGFTRQQVL 300 LAPVFLMLLKTLVDVTLPEGHAMRDLMDFIGNPIVALLAALLLAIWTFGIARGFTRQQVL Sbjct: 241 LAPVFLMLLKTLVDVTLPEGHAMRDLMDFIGNPIVALLAALLLAIWTFGIARGFTRQQVL 300 Query: 301 KFVDQSLAPTAAIVLIIGAGGGFKQMLVNSGVGTAIGQLAVHAQISPLLLAWFVAGVIRV 360 KFVDQSLAPTAAIVLIIGAGGGFKQMLVNSGVGTAIGQLAVHAQISPLLLAWFVAGVIRV Sbjct: 301 KFVDQSLAPTAAIVLIIGAGGGFKQMLVNSGVGTAIGQLAVHAQISPLLLAWFVAGVIRV 360 Query: 361 ATGSATVATITGAGIVAPLVALIPGTNKELLVLATGAGSLILSHVNDAGFWLVKEYFNMS 420 ATGSATVATITGAGIVAPLVALIPGTNKELLVLATGAGSLILSHVNDAGFWLVKEYFNMS Sbjct: 361 ATGSATVATITGAGIVAPLVALIPGTNKELLVLATGAGSLILSHVNDAGFWLVKEYFNMS 420 Query: 421 VGETFKTWTVMETLISVVAIIFIMLLNLVV 450 VGETFKTWTVMETLISVVAIIFIMLLNLVV Sbjct: 421 VGETFKTWTVMETLISVVAIIFIMLLNLVV 450 >gi|300311081|ref|YP_003775173.1| high-affinity gluconate transporter (GNT-III system) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 481 bits (1238), Expect = e-137, Method: Compositional matrix adjust. Identities = 240/433 (55%), Positives = 321/433 (74%), Gaps = 1/433 (0%) Query: 6 HDATLLFNAVIAILALILMITRLRIHPFIALTITSGLLGLISGMPLPKIVKSFQDGFGGV 65 + LL A++A++ALI++I + +++PFI L + S +LGL GMP+ IVK+F+ G G Sbjct: 5 QGSALLVYALVAVIALIVLIAKFKMNPFIVLIVVSLVLGLAVGMPMGNIVKAFETGVGNA 64 Query: 66 LGFVGIVLGLGTMLGKLMAESGGADQIAQTLVRAFGRERVHWAMMMGAFLVGIPLFFEIG 125 LG + +V+GLGTMLGK+MAESGGA++IA T+++AFG + VHWAMM AF+VG+P+FFE+G Sbjct: 65 LGHIALVVGLGTMLGKMMAESGGAERIANTMIKAFGEKNVHWAMMTVAFIVGLPVFFEVG 124 Query: 126 FVLLIPLVFIVARRSGVPLFKIGMPMLASLSVMHGLVPPHPGPLLAIGIFGADIGKTIFY 185 FVLL+P+ F VA+R+G + +G+PM+A LSV+HGL+PPHP LLA+ + ADIG+TI Y Sbjct: 125 FVLLVPIAFNVAKRTGTNMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYSADIGRTILY 184 Query: 186 GLLVGLPTVIIAGPMFGAFISRYVDIKPSEELMAQLARE-PQTTKLPGFATTLITVLAPV 244 L+VG+PT IIAGP+FG IS+ V P L++Q E + +LPGF TL T+L PV Sbjct: 185 ALIVGIPTAIIAGPIFGKLISKVVIPNPDNPLISQFVDEGKKDRELPGFGITLFTILLPV 244 Query: 245 FLMLLKTLVDVTLPEGHAMRDLMDFIGNPIVALLAALLLAIWTFGIARGFTRQQVLKFVD 304 LML+ + D+ D + IGN ++ALL A L++ WTFG ARGF Q+LKF + Sbjct: 245 ALMLIGSWADLFFAPKTFANDFLRLIGNSVIALLIATLVSFWTFGRARGFGADQILKFTN 304 Query: 305 QSLAPTAAIVLIIGAGGGFKQMLVNSGVGTAIGQLAVHAQISPLLLAWFVAGVIRVATGS 364 + LAP A+I L++GAG GF ++L++ GV AI +A A +SPL+L WFVA +IRVATGS Sbjct: 305 ECLAPIASITLVVGAGAGFGRILMDGGVSKAIVGIATDAHLSPLILGWFVAALIRVATGS 364 Query: 365 ATVATITGAGIVAPLVALIPGTNKELLVLATGAGSLILSHVNDAGFWLVKEYFNMSVGET 424 ATVA T GIVAP+V+ + G EL+VLATGAGSLILSHVND GFWLVKEYFNM+V +T Sbjct: 365 ATVAMTTACGIVAPIVSTVGGVRPELMVLATGAGSLILSHVNDGGFWLVKEYFNMTVPQT 424 Query: 425 FKTWTVMETLISV 437 FKTWTVMETL+SV Sbjct: 425 FKTWTVMETLVSV 437 >gi|300311416|ref|YP_003775508.1| gluconate transporter family protein [Herbaspirillum seropedicae SmR1] Length = 456 Score = 327 bits (838), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 180/421 (42%), Positives = 282/421 (66%), Gaps = 3/421 (0%) Query: 21 LILMITRLRIHPFIALTITSGLLGLISGMPLPKIVKSFQDGFGGVLGFVGIVLGLGTMLG 80 ++++I+ L I PF+++ I + + G+ +G+PL I K+F G G +LG GI++ LG MLG Sbjct: 27 IVVLISFLSIAPFLSILIGTFVAGIGAGLPLEDIAKAFSKGAGSLLGEAGIIIALGAMLG 86 Query: 81 KLMAESGGADQIAQTLVRAFGRERVHWAMMMGAFLVGIPLFFEIGFVLLIPLVFIVARRS 140 LMAESG AD+I TL+R + W M + A +VG+PLFFE+G V++ P++F++ARRS Sbjct: 87 ALMAESGAADRIVNTLLRYARGSFIPWMMALVALVVGLPLFFEVGLVMMAPIIFVMARRS 146 Query: 141 GVPLFKIGMPMLASLSVMHGLVPPHPGPLLAIGIFGADIGKTIFYGLLVGLPTVIIAGPM 200 +P+ ++ +P LA ++ +H L+PPHPGPL+A+ AD+G T+ G +V +P VIIAGP+ Sbjct: 147 ELPIMRVAIPALAGMTTLHALLPPHPGPLIAVSALHADLGTTMLLGFIVAIPAVIIAGPL 206 Query: 201 FGAFISRYVDIKPSEELMAQL-AREPQTTKLPGFATTLITVLAPVFLMLLKTLVDVTLPE 259 +G FI+ +++ +++ ARE Q+ P F L+T+L PV LML +T+ + + Sbjct: 207 YGNFIAPRLNLAEPDQIGKLFSAREGQSQ--PSFLVALVTILLPVVLMLGRTVARIWVTP 264 Query: 260 GHAMRDLMDFIGNPIVALLAALLLAIWTFGIARGFTRQQVLKFVDQSLAPTAAIVLIIGA 319 + ++++F G PI+AL +L A+ G +G +R +V + ++L P A ++L IGA Sbjct: 265 KTELFEMLNFFGEPIIALTVTVLFAVVVLGWGQGSSRFEVGATLRRALPPIAGLLLTIGA 324 Query: 320 GGGFKQMLVNSGVGTAIGQLAVHAQISPLLLAWFVAGVIRVATGSATVATITGAGIVAPL 379 GGG KQ L+++G+ I ++A + +LLAW +A +R ATGSATVAT AGIVAPL Sbjct: 325 GGGLKQTLLSAGISDTITKIADGTHMPLILLAWIIAVALRQATGSATVATTATAGIVAPL 384 Query: 380 VALIPGTNKELLVLATGAGSLILSHVNDAGFWLVKEYFNMSVGETFKTWTVMETLISVVA 439 VA + T+ L+ LA GAGS+ HVNDAGFW+VKEYF +++ +T TW+V++TL+SV+ Sbjct: 385 VAGLSATHNSLMALAIGAGSVFFCHVNDAGFWMVKEYFGLNLKQTLATWSVIQTLVSVIG 444 Query: 440 I 440 + Sbjct: 445 L 445 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.145 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,193,583 Number of extensions: 47319 Number of successful extensions: 294 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 287 Number of HSP's successfully gapped: 3 Length of query: 450 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 362 Effective length of database: 1,203,190 Effective search space: 435554780 Effective search space used: 435554780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)