BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0462.p (520 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 1054 0.0 gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 161 1e-40 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 142 9e-35 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 128 1e-30 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 89 6e-19 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 1054 bits (2725), Expect = 0.0, Method: Compositional matrix adjust. Identities = 517/520 (99%), Positives = 518/520 (99%) Query: 1 MSTSALARYMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAA 60 MSTSALARYMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAA Sbjct: 1 MSTSALARYMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAA 60 Query: 61 LASIAGAVKAAKAAPQEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRASVWAERIKH 120 LASIAGAVKAAKAAPQEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRASVWAERIKH Sbjct: 61 LASIAGAVKAAKAAPQEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRASVWAERIKH 120 Query: 121 EHDGNAIYRRTGTPVHPMSPLCKLMWMRHEKPEVFHRAARFVGIKEYLLYQLFGQWVVDH 180 EHDGNAIYRRTGTPVHPMSPLCKLMWMRHEKP+VFHRAARFVGIKEYLLYQLFGQWVVDH Sbjct: 121 EHDGNAIYRRTGTPVHPMSPLCKLMWMRHEKPDVFHRAARFVGIKEYLLYQLFGQWVVDH 180 Query: 181 SIASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSPEMAQRLGLSVDTPF 240 SIASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALS EMAQRLGLSVDTPF Sbjct: 181 SIASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPF 240 Query: 241 IIGANDGVLSNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGRLFCYALTEKHWVVG 300 IIGANDGVLSNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGRLFCYALTEKHWVVG Sbjct: 241 IIGANDGVLSNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGRLFCYALTEKHWVVG 300 Query: 301 GPVNNGGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPGAEGLLFHPFMAGER 360 GPVNNGGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPGAEGLLFHPFMAGER Sbjct: 301 GPVNNGGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPGAEGLLFHPFMAGER 360 Query: 361 APLWNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALEELVGPTKRMMATGGFA 420 APLWNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALEELVGPTKRMMATGGFA Sbjct: 361 APLWNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALEELVGPTKRMMATGGFA 420 Query: 421 RSALWRQMMADIFNREVVVPESVESSCLGAAVLGAWALGLVPSLSVISGMVGSTNHHAPE 480 RSALWRQMMADIFNREVVVPESVESSCLGAAVLGAWALGLVPSLSVISGMVGSTNHHAPE Sbjct: 421 RSALWRQMMADIFNREVVVPESVESSCLGAAVLGAWALGLVPSLSVISGMVGSTNHHAPE 480 Query: 481 AEAVAVYGRLQPIFAAIPAKLEAEYHAIAAFQREAGRSHP 520 AEAVAVYGRLQPIFAAIPAKLEAEYHAIAAFQREAGR HP Sbjct: 481 AEAVAVYGRLQPIFAAIPAKLEAEYHAIAAFQREAGRPHP 520 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 161 bits (407), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 133/472 (28%), Positives = 210/472 (44%), Gaps = 28/472 (5%) Query: 9 YMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAV 68 Y+L +D GT+S++S++F G +VA +E+P + +PG E DP + + L + + Sbjct: 3 YLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQKVL 62 Query: 69 KAAKAAPQEIALLSFSAAMHSVIALD-AENRLLSNSITWGDIRASVWAERIKHEHDGNAI 127 + E+ L + + + + A + + N++ W D RA V ++ G AI Sbjct: 63 AQSGIKASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGEAI 122 Query: 128 YRRTGTPVHPMSPLCKLMWMRHEKPEVFHRAAR----FVGIKEYLLYQLFGQW--VVDHS 181 ++TG + P KL W+ + RA R F + +L++ L G V D S Sbjct: 123 QKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSDVS 182 Query: 182 IASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSPEMAQRLGLSVDTPFI 241 AS T ++N+ AWD+ L LG+ LP P+ H + AQ LG +V I Sbjct: 183 NASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEID---AQHLGAAVKIGGI 239 Query: 242 IGANDGVLSNLGVNAIEIGHVAVTIGTSGAMRT-VIDEPRTDPQGRLF---CYALTEKHW 297 G L G + G T GT M D+ T G + C +K + Sbjct: 240 AGDQQSAL--FGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQKQY 297 Query: 298 VVGGPVNNGGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPGAEGLLFHPFMA 357 + G V GG + +W+RD L + + E L E + G++F P Sbjct: 298 ALEGSVFIGGAVVQWLRDGLKAIKTST-------DVEGLACSVED----SGGVVFVPSFT 346 Query: 358 GERAPLWNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPAL-EELVGPTKRMMAT 416 G AP WN +G+ GL+ H+ RAALE + F ++L A+ + V P + Sbjct: 347 GLGAPYWNPSAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVD 406 Query: 417 GGFARSALWRQMMADIFNREVVVPESVESSCLGAAVLGAWALGLVPSLSVIS 468 GG A + L Q AD+ V+ P+ E++ LGAA L A G+ LS +S Sbjct: 407 GGAAANNLLLQFQADLLGIPVIRPQVTETTALGAAYLAGIATGVYRDLSELS 458 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 142 bits (357), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 135/451 (29%), Positives = 202/451 (44%), Gaps = 20/451 (4%) Query: 11 LGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAG-AVK 69 LG+D+GT+ K+VV G +VA V P +EQ P A ++A + Sbjct: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLRTQ 62 Query: 70 AAKAAPQEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRASVWAERIKHEHDGNAIYR 129 I + S MH + LD + +L +I W D R++ E + E ++ Sbjct: 63 LGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSA--PECAELESRAPRLHG 120 Query: 130 RTGTPVHPMSPLCKLMWMRHEKPEVFHRAARFVGIKEYLLYQLFGQWVVDHSIASATGMF 189 G P KL+W+ +P++F R A + K++L ++ G+ V D S A+ T Sbjct: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL 180 Query: 190 NLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSPEMAQRLGLSVDTPFIIGANDGVL 249 ++ W LA G+R DQ+P V + +L PE+AQ GL D GA DG Sbjct: 181 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAA 240 Query: 250 SNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGR---LFCYALTEKHWVVGGPVNNG 306 S +G+ A++ G +++GTSG + V D R +P GR FC+ L ++ W + + Sbjct: 241 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNP-GRAIHAFCHTLPQR-WHQMSVMLSA 298 Query: 307 GNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPGAEGLLFHPFMAGERAPLWNA 366 + RW + E + E E L A P LF P+++GER P + Sbjct: 299 ASCLRWFCRLCSVDEKSLLAE-----IEQLDEQACNNAP-----LFLPYLSGERTPHNDP 348 Query: 367 DLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALEELVGPTKRMMATGGFARSALWR 426 G F GL H + + A LEGV F + L AL + GG ARSA W Sbjct: 349 YATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWA 408 Query: 427 QMMADIFNREVVVPESVES-SCLGAAVLGAW 456 Q++AD +V E+ LGAA L AW Sbjct: 409 QLLADALQVRIVTHVGSEAGGALGAARL-AW 438 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 122/452 (26%), Positives = 190/452 (42%), Gaps = 26/452 (5%) Query: 11 LGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAVKA 70 LG+D+GT+ K V+ D V+A + V P +EQ P+ A L +IA Sbjct: 4 LGIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAALRTQ 63 Query: 71 AKAAPQEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRASVWAERIKHEH---DGNAI 127 A A Q + + S MH LD +L +I W D+RA AE ++ E D AI Sbjct: 64 APQAFQALRGIGLSGQMHGATLLDRNGNVLRPAILWNDMRAH--AECVELEALVPDAVAI 121 Query: 128 YRRTGTPVHPMSPLCKLMWMRHEKPEVFHRAARFVGIKEYLLYQLFGQWVVDHSIASATG 187 TG P KL+W+ +P V+ + + K++L ++L G++V + S A+ T Sbjct: 122 ---TGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDFLGWKLTGEFVSEMSDAAGTL 178 Query: 188 MFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSPEMAQRLGLSVDTPFIIGANDG 247 ++ W + L G+ +P V + L E+ + G+S GA D Sbjct: 179 WLDVARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGISGPVVVAGGAGDN 238 Query: 248 VLSNLGVNAIEIGHVAVTIGTSGAM--RTVIDEPRTDPQGRLFCYALTEKHWVVGGPVNN 305 S +G+ I G +++G+SG + T P FC+ L + W + + Sbjct: 239 AASAVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCLPGQ-WHQMSVILS 297 Query: 306 GGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPG--AEGLLFHPFMAGERAPL 363 W+ A P L AE++ G A+ LF P++ GER P Sbjct: 298 AAASLHWLSGVTAR------------PVAELVSGAERLSAGQQAQAPLFLPYLNGERTPH 345 Query: 364 WNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALEELVGPTKRMMATGGFARSA 423 +A +G FG+ H H+ A +EGV F + AL+ + GG +RSA Sbjct: 346 NDAAAKGVLFGMTPAHESAHLAYAVMEGVAFAMADGYAALQAAGTTLESAAFVGGGSRSA 405 Query: 424 LWRQMMADIFNREVVVPESVE-SSCLGAAVLG 454 W + A + E + LGAA LG Sbjct: 406 FWGVLCATALGIPLRRHAGAEVGAALGAARLG 437 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 89.4 bits (220), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 125/502 (24%), Positives = 195/502 (38%), Gaps = 44/502 (8%) Query: 9 YMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEP-GMAEQDPEQIVAAALASIAGA 67 Y++G+D+GT S ++ VF G+++A + + E EQ QI A ++ A Sbjct: 4 YLIGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAVRAA 63 Query: 68 VKAAKAAPQEIALLSFSAAMHSVI-----ALDAENRLLSNSITWGDIRASVWAERIKHEH 122 V + P +A + A V+ + + I W D RA A+RI Sbjct: 64 VTKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRINRM- 122 Query: 123 DGNAIYRRTGTPVHPMSPLCKLMWMRHEKPEVFHRAARFVGIKEYLLYQLFGQWVVDHSI 182 G+A+ G + P KL+W++ PEV+ AA F + ++L ++ G Sbjct: 123 -GHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQRSSCT 181 Query: 183 ASATGMFNLRELAWDQ--------GALALLGVRPDQLPTPVPTTHRLPALSPEMAQRLGL 234 + + E WD G LA G P T L+ AQ +GL Sbjct: 182 VTCKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMGL 241 Query: 235 SVDTPFIIGANDGVLSNLGVNAIEIGH------VAVTIGTSGAMRTVIDEPRTDPQ--GR 286 G D +G A G +A GTS T +P P G Sbjct: 242 RPGIAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWGP 301 Query: 287 LFCYALTEKHWV-VGGPVNNGGNIFRWVRDELATAEA--AAAREEGVDP-YEALTRIAEK 342 + A+ W+ GG G I +R AT +A AAR + P + A +A Sbjct: 302 YY-KAMAPGMWLNEGGQSAAGAAIDHLLRLHPATPQAREEAARAQMELPQWLAQQALAAV 360 Query: 343 VRPG-----AEGLLFHPFMAGERAPLWNADLRGSFFGLALHHGKHHMIRAALEGVI---F 394 P A L P G R+PL + R + GL + H ++ + G+ + Sbjct: 361 DHPSEAVWLAGQLTVVPEFLGNRSPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGY 420 Query: 395 NLYSILPALEELVGPTKRMMATGGFARSALWRQMMADIFNREVVVPESVESSCLGAAVLG 454 L I+ A + + +GG L RQ++AD V + S E LG+A+L Sbjct: 421 GLRQIIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEPVLLGSAMLA 480 Query: 455 AWALGL-------VPSLSVISG 469 A A GL +P++S ++G Sbjct: 481 AVAAGLHADLQSAMPAMSSVAG 502 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,533,958 Number of extensions: 62645 Number of successful extensions: 195 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 182 Number of HSP's successfully gapped: 5 Length of query: 520 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 431 Effective length of database: 1,198,455 Effective search space: 516534105 Effective search space used: 516534105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)