BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0461.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 531 e-152 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 139 2e-34 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 138 5e-34 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 135 4e-33 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 134 6e-33 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 531 bits (1368), Expect = e-152, Method: Compositional matrix adjust. Identities = 261/264 (98%), Positives = 262/264 (99%) Query: 1 VTTKTTPLPAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVA 60 +TTKTTPLPAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVA Sbjct: 1 MTTKTTPLPAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVA 60 Query: 61 AQLRAEGHTIHESSFDATSSEEVKVAVQKIESEIGSIGILVNNAGIQRRNPLEEFKESDW 120 AQLRAEGHTIHESSFDATSSEEVK AVQKIESEIG IGILVNNAGIQRRNPLEEFKESDW Sbjct: 61 AQLRAEGHTIHESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDW 120 Query: 121 HDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTK 180 HDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTK Sbjct: 121 HDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTK 180 Query: 181 GMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVF 240 GMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVF Sbjct: 181 GMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVF 240 Query: 241 LASDASRFVNGHILYVDGGVTATL 264 LASDASRFVNGHILYVDGGVTATL Sbjct: 241 LASDASRFVNGHILYVDGGVTATL 264 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 139 bits (350), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 86/255 (33%), Positives = 132/255 (51%), Gaps = 5/255 (1%) Query: 9 PAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGH 68 P+ LQ F EGK+ ++TG++ GIG A AR + GA ++L RDA L V L E Sbjct: 6 PSRLQRF--EGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERT 63 Query: 69 TIHESSFDATSSEEVKVAVQKIESEIGSIGILVNNAGIQRRNPLEEFKESDWHDLISTNL 128 E+ D + E+V+ V G + +LV++AG+ + E DWH + +TN+ Sbjct: 64 AARET--DVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNV 121 Query: 129 NSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGK 188 N VF+ A+ H+ + G I+N+ SV ++ Y A+KGAV T+ MA+D+G+ Sbjct: 122 NGVFYGAREALPHL-EKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGR 180 Query: 189 YGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRF 248 G+++N + P T M + +D D R R D ++ FLAS + F Sbjct: 181 KGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASF 240 Query: 249 VNGHILYVDGGVTAT 263 VNG L VDGG+ A+ Sbjct: 241 VNGVNLPVDGGLMAS 255 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 138 bits (347), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 93/249 (37%), Positives = 125/249 (50%), Gaps = 6/249 (2%) Query: 16 SLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSF 75 +L+GK+ALITG+S GIG A A A+ GAA++L R L VAA + A+G + Sbjct: 3 TLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAG 62 Query: 76 DATSSEEVKVAVQKIESEIGSIGILVNNAG-IQRRNPLEEFKESDWHDLISTNLNSVFFA 134 D + + V E G + I +NNAG + PL E W D + NL S F Sbjct: 63 DVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLG 122 Query: 135 AQAVARHMIPRGAGKIINICS-VQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQV 193 A+ M+ RGAG I+ S V S G PG+A Y A K + KG+A D+ GI+ Sbjct: 123 ARCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRA 182 Query: 194 NGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHI 253 N L PG T+M D + W G +R E++ AA+FLAS + FV G Sbjct: 183 NALLPGGVDTDMG----GDPQQKQWAAGLHAMKRIAQPEEIAAAALFLASPMASFVTGTA 238 Query: 254 LYVDGGVTA 262 LY DGG +A Sbjct: 239 LYADGGNSA 247 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 135 bits (339), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 85/246 (34%), Positives = 135/246 (54%), Gaps = 3/246 (1%) Query: 16 SLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSF 75 +L+G++AL+TG+S GIG A+A +A+ GA ++ AA+ L A G + Sbjct: 5 NLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAV 64 Query: 76 DATSSEEVKVA-VQKIESEIGSIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFA 134 + E VA V++++ + GS+ ILVNNAGI + K+ +W +I+TNL +V Sbjct: 65 LNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRL 124 Query: 135 AQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVN 194 ++AV R M+ G+IINI SV E G PG Y A K V ++ +A + G I VN Sbjct: 125 SRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVN 184 Query: 195 GLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHIL 254 + PG+ T+M AL + + + ++ + P R G E++ A FLAS + ++ G L Sbjct: 185 CVAPGFIDTDMTRAL--NEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTL 242 Query: 255 YVDGGV 260 V+GG+ Sbjct: 243 NVNGGM 248 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 134 bits (338), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 78/245 (31%), Positives = 128/245 (52%), Gaps = 3/245 (1%) Query: 17 LEGKLALITGSSGGIGLALARGMAQAGAAIVLN-GRDAGKLAAVAAQLRAEGHTIHESSF 75 + ++A +TG GGIG A+ + + G +V G ++ + A +R +G IH S Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 Query: 76 DATSSEEVKVAVQKIESEIGSIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAA 135 + ++ E K A +K+ +EIG + +LVNNAGI R + + DW +I TNLNS+F Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 136 QAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNG 195 + V M RG G+IINI SV + G+ G Y+ K + FT +A + G+ VN Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 Query: 196 LGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILY 255 + PGY T+M A+ + + +V P +R G+ E++ ++AS + G Sbjct: 181 VSPGYIGTDMVRAI--RPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFS 238 Query: 256 VDGGV 260 ++GG+ Sbjct: 239 LNGGL 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 785,068 Number of extensions: 30692 Number of successful extensions: 278 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 49 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)