BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0460.p (226 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 461 e-131 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 122 3e-29 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 119 2e-28 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 117 9e-28 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 115 3e-27 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 461 bits (1185), Expect = e-131, Method: Compositional matrix adjust. Identities = 224/226 (99%), Positives = 224/226 (99%) Query: 1 MAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSFDATSSEEVKVAVQKIESEIGSIG 60 MAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSFDATSSEEVK AVQKIESEIG IG Sbjct: 39 MAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSFDATSSEEVKAAVQKIESEIGPIG 98 Query: 61 ILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQS 120 ILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQS Sbjct: 99 ILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQS 158 Query: 121 ELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDW 180 ELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDW Sbjct: 159 ELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDW 218 Query: 181 LVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDGGVTATL 226 LVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDGGVTATL Sbjct: 219 LVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDGGVTATL 264 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 122 bits (305), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 67/200 (33%), Positives = 106/200 (53%), Gaps = 2/200 (1%) Query: 23 AQLRAEGHTIHESSFDATSSEEVKVAVQKIESEIGSIGILVNNAGIQRRNPLEEFKESDW 82 A +R +G IH S + ++ E K A +K+ +EIG + +LVNNAGI R + + DW Sbjct: 46 ATMREQGFDIHASEGNVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDW 105 Query: 83 HDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTK 142 +I TNLNS+F + V M RG G+IINI SV + G+ G Y+ K + FT Sbjct: 106 DAVIDTNLNSLFNVTKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTM 165 Query: 143 GMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVF 202 +A + G+ VN + PGY T+M A+ + + +V P +R G+ E++ + Sbjct: 166 ALAQEVATKGVTVNTVSPGYIGTDMVRAI--RPEVLEKIVSGIPVKRLGEPEEIASIVAW 223 Query: 203 LASDASRFVNGHILYVDGGV 222 +AS + G ++GG+ Sbjct: 224 IASSEGGYATGSDFSLNGGL 243 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 119 bits (297), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 65/200 (32%), Positives = 100/200 (50%), Gaps = 1/200 (0%) Query: 25 LRAEGHTIHESSFDATSSEEVKVAVQKIESEIGSIGILVNNAGIQRRNPLEEFKESDWHD 84 L A GH D S E + A IE + G +LVNNAG+ R ++ +W Sbjct: 52 LVAAGHRAAPLQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQ 111 Query: 85 LISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGM 144 ++ N+ V A Q AR M+ + G+I+NI S+ G Y +K AV T+ M Sbjct: 112 TMNVNVTGVLLAGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQM 171 Query: 145 AIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLA 204 AI+ +YGI VN + PG T M A+ ++ + P RR+G +++ A +LA Sbjct: 172 AIELAQYGITVNSVAPGPVDTPMTQAMHSEQTRESYY-RLVPMRRYGTTDEIASAVCYLA 230 Query: 205 SDASRFVNGHILYVDGGVTA 224 ++ S ++ GH++ VDGG A Sbjct: 231 AEESAYITGHVIPVDGGFVA 250 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 117 bits (292), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 79/225 (35%), Positives = 107/225 (47%), Gaps = 6/225 (2%) Query: 2 AQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSFDATSSEEVKVAVQKIESEIGSIGI 61 A+ GAA++L R L VAA + A+G + D + + V E G + I Sbjct: 27 ARHGAALILTARRQDALEQVAALICAQGGRASVVAGDVGEAATHERGVAMALDEFGGLDI 86 Query: 62 LVNNAG-IQRRNPLEEFKESDWHDLISTNLNSVFFAAQAVARHMIPRGAGKIINICS-VQ 119 +NNAG + PL E W D + NL S F A+ M+ RGAG I+ S V Sbjct: 87 AINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLGARCQIPAMLARGAGAIVFTSSFVG 146 Query: 120 SELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTD 179 S G PG+A Y A K + KG+A D+ GI+ N L PG T+M D + Sbjct: 147 SSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRANALLPGGVDTDMG----GDPQQKQ 202 Query: 180 WLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDGGVTA 224 W G +R E++ AA+FLAS + FV G LY DGG +A Sbjct: 203 WAAGLHAMKRIAQPEEIAAAALFLASPMASFVTGTALYADGGNSA 247 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 115 bits (287), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 71/221 (32%), Positives = 110/221 (49%), Gaps = 3/221 (1%) Query: 5 GAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSFDATSSEEVKVAVQKIESEIGSIGILVN 64 GA ++L RDA L V L E E+ D + E+V+ V G + +LV+ Sbjct: 38 GARVLLADRDAAALGKVFDSLPPERTAARET--DVSHHEQVRQLVDFAIERFGQLDVLVS 95 Query: 65 NAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGR 124 +AG+ + E DWH + +TN+N VF+ A+ H+ + G I+N+ SV Sbjct: 96 DAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAREALPHL-EKTRGCIVNVASVSGLAAD 154 Query: 125 PGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGR 184 ++ Y A+KGAV T+ MA+D+G+ G+++N + P T M + +D D R Sbjct: 155 WNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCPSLTHTAMTADMADDPPLLDKFAER 214 Query: 185 TPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDGGVTAT 225 R D ++ FLAS + FVNG L VDGG+ A+ Sbjct: 215 IALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGGLMAS 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 699,782 Number of extensions: 28292 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 47 Length of query: 226 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 144 Effective length of database: 1,231,600 Effective search space: 177350400 Effective search space used: 177350400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)