BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0459.p
         (271 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb...   544   e-156
gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir...    90   2e-19

>gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 345

 Score =  544 bits (1402), Expect = e-156,   Method: Compositional matrix adjust.
 Identities = 268/269 (99%), Positives = 269/269 (100%)

Query: 3   SDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPHVQGAFRQEIIIERSQ 62
           +DIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPHVQGAFRQEIIIERSQ
Sbjct: 77  TDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPHVQGAFRQEIIIERSQ 136

Query: 63  AHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPIGALVVVAARRAGALE 122
           AHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPIGALVVVAARRAGALE
Sbjct: 137 AHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPIGALVVVAARRAGALE 196

Query: 123 VVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLFEASGNERALVGALE 182
           VVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLFEASGNERALVGALE
Sbjct: 197 VVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLFEASGNERALVGALE 256

Query: 183 ALRPQAIIVQVGLGGDIQFPANLLVGKELEWRGAFRFHEEFAMAVELMNRKLVDVKPLIT 242
           ALRPQAIIVQVGLGGDIQFPANLLVGKELEWRGAFRFHEEFAMAVELMNRKLVDVKPLIT
Sbjct: 257 ALRPQAIIVQVGLGGDIQFPANLLVGKELEWRGAFRFHEEFAMAVELMNRKLVDVKPLIT 316

Query: 243 ATYPIAQAVEAFEIAGDRSRMMKVQISFD 271
           ATYPIAQAVEAFEIAGDRSRMMKVQISFD
Sbjct: 317 ATYPIAQAVEAFEIAGDRSRMMKVQISFD 345


>gi|300312693|ref|YP_003776785.1| D-xylulose reductase
           [Herbaspirillum seropedicae SmR1]
          Length = 345

 Score = 90.1 bits (222), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 78/272 (28%), Positives = 126/272 (46%), Gaps = 7/272 (2%)

Query: 3   SDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPHVQGAFRQEIIIERSQ 62
           S +  G R+ + P  P         G  N    +RF+     TP + G     ++   + 
Sbjct: 77  SHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWA----TPPIHGCLTGSVVHPAAF 132

Query: 63  AHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPIGALVVVAARRAGALE 122
            + + D+VS  E A+ EPLS+ L A  +A    G   +V G G IGA+  +AA   GA  
Sbjct: 133 TYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAMTALAALAGGAAR 192

Query: 123 VVVTDVASMPLQAALQVGADSAINMA-ETPAALKAYAADKGTFDVLFEASGNERALVGAL 181
           V++ DV +  L       A   +++  ET   +   A D    DV+FEASG+       L
Sbjct: 193 VILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQATDGWGADVVFEASGHAGVYQTLL 252

Query: 182 EALRPQAIIVQVGL-GGDIQFPANLLVGKELEWRGAFRFHEEFAMAVELMNRKLVDVKPL 240
           + + P    V VG+    +      +  KE+     FR+   F  A+ L++  ++DVKP 
Sbjct: 253 DLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYANIFPRALALISSGMIDVKPF 312

Query: 241 ITATYPIAQAVEAF-EIAGDRSRMMKVQISFD 271
           I+  +P +Q++ AF E A  R + +K+QI  +
Sbjct: 313 ISRKFPFSQSIRAFEEAASGRPQDVKIQIEME 344


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.135    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 769,153
Number of extensions: 29564
Number of successful extensions: 120
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 116
Number of HSP's successfully gapped: 3
Length of query: 271
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 187
Effective length of database: 1,222,130
Effective search space: 228538310
Effective search space used: 228538310
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 130 (54.7 bits)