BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0458.p (503 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310428|ref|YP_003774520.1| methyl-accepting chemotaxis tra... 991 0.0 gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis tra... 260 2e-70 gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis tra... 198 7e-52 gi|300311931|ref|YP_003776023.1| methyl-accepting chemotaxis II ... 195 7e-51 gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis tra... 192 4e-50 >gi|300310428|ref|YP_003774520.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 517 Score = 991 bits (2561), Expect = 0.0, Method: Compositional matrix adjust. Identities = 501/503 (99%), Positives = 502/503 (99%) Query: 1 LSVLILLALLGLVATAAVSLRQIENVYTAANYAQVNTVPSVTTLDDAQAAFNAIQGRIYQ 60 LSVLILLALLGLVATAAVSLRQIENVYTAANYAQVNTVPSVTTLDDAQAAFNAIQGRIYQ Sbjct: 15 LSVLILLALLGLVATAAVSLRQIENVYTAANYAQVNTVPSVTTLDDAQAAFNAIQGRIYQ 74 Query: 61 YLVNPDPRDRSRLIKEMDVQRARMQRHLLTYANAYITDETDGAMLADDRAHIAAFEEVVR 120 YLVNPDPRDRSRLIKEMDVQRARMQRHL TYANAYITDETDGAMLADDRAHIAAFEEVVR Sbjct: 75 YLVNPDPRDRSRLIKEMDVQRARMQRHLHTYANAYITDETDGAMLADDRAHIAAFEEVVR 134 Query: 121 RLLAVAEEDRVEDSLGLSVLEFRAALDTVQASFARHRSYNVALGDASALTAREIIGQALE 180 RLLAVAEEDRVEDSLGLSVLEFRAA+DTVQASFARHRSYNVALGDASALTAREIIGQALE Sbjct: 135 RLLAVAEEDRVEDSLGLSVLEFRAAIDTVQASFARHRSYNVALGDASALTAREIIGQALE 194 Query: 181 TVALVAGGVLLILLGAGWLLTRTLLRQMGGEPGDVVTVMQRLSDGDLVQTITLKPGCRDS 240 TVALVAGGVLLILLGAGWLLTRTLLRQMGGEPGDVVTVMQRLSDGDLVQTITLKPGCRDS Sbjct: 195 TVALVAGGVLLILLGAGWLLTRTLLRQMGGEPGDVVTVMQRLSDGDLVQTITLKPGCRDS 254 Query: 241 MMHAIQKMMTRLAQVVGEVRGNADGLVSTAEEVSATAQSLSRASTEQANGVEQTSEALAQ 300 MMHAIQKMMTRLAQVVGEVRGNADGLVSTAEEVSATAQSLSRASTEQANGVEQTSEALAQ Sbjct: 255 MMHAIQKMMTRLAQVVGEVRGNADGLVSTAEEVSATAQSLSRASTEQANGVEQTSEALAQ 314 Query: 301 IHALLQSTAQNARLTDQIAAAAALEARQCAETVKQMVAAIQQIAGKIAVIDDIAYQTNLL 360 IHALLQSTAQNARLTDQIAAAAALEARQCAETVKQMVAAIQQIAGKIAVIDDIAYQTNLL Sbjct: 315 IHALLQSTAQNARLTDQIAAAAALEARQCAETVKQMVAAIQQIAGKIAVIDDIAYQTNLL 374 Query: 361 ALNATIEAAHAGEHGSGFAVVAAEVRRLAERSQSAAQEIDEVARANVTLACAAERQLSDM 420 ALNATIEAAHAGEHGSGFAVVAAEVRRLAERSQSAAQEIDEVARANVTLACAAERQLSDM Sbjct: 375 ALNATIEAAHAGEHGSGFAVVAAEVRRLAERSQSAAQEIDEVARANVTLACAAERQLSDM 434 Query: 421 VPTITRTSVLVQDIVQSSEQQSTAVSQISTAASQLAQVIQQNSLSSEELAVTSEELNRRA 480 VPTITRTSVLVQDIVQSSEQQSTAVSQISTAASQLAQVIQQNSLSSEELAVTSEELNRRA Sbjct: 435 VPTITRTSVLVQDIVQSSEQQSTAVSQISTAASQLAQVIQQNSLSSEELAVTSEELNRRA 494 Query: 481 LLLQTAVGFFQCQLARGMQARLA 503 LLLQTAVGFFQCQLARGMQARLA Sbjct: 495 LLLQTAVGFFQCQLARGMQARLA 517 >gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 595 Score = 260 bits (665), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 149/312 (47%), Positives = 214/312 (68%) Query: 190 LLILLGAGWLLTRTLLRQMGGEPGDVVTVMQRLSDGDLVQTITLKPGCRDSMMHAIQKMM 249 +++L WL R + Q+GGEP D ++V++R+++GDL + L G + S++++ Q+M+ Sbjct: 244 VVVLFACLWLSYRIIRSQIGGEPRDAMSVLRRVAEGDLTVVVPLPKGDKLSVLYSTQQMI 303 Query: 250 TRLAQVVGEVRGNADGLVSTAEEVSATAQSLSRASTEQANGVEQTSEALAQIHALLQSTA 309 + V+G+V G A L S +E++S+++Q+LS + +QA VE+TS ++ +I A + A Sbjct: 304 NKWTDVIGDVSGTATSLASASEQISSSSQALSHNAAQQAANVEETSASVEEITATIAQNA 363 Query: 310 QNARLTDQIAAAAALEARQCAETVKQMVAAIQQIAGKIAVIDDIAYQTNLLALNATIEAA 369 NAR TD IA+ +A A + E V++ VAA++QIA KI +IDDIAYQTNLLALNA IEAA Sbjct: 364 DNARTTDGIASLSASAATEGGEAVRETVAAMKQIASKIGIIDDIAYQTNLLALNAAIEAA 423 Query: 370 HAGEHGSGFAVVAAEVRRLAERSQSAAQEIDEVARANVTLACAAERQLSDMVPTITRTSV 429 AGEHG GFAVVAAEVR+LAERSQSAAQEI VA +V LA A L+ MVP+I +T+ Sbjct: 424 RAGEHGKGFAVVAAEVRKLAERSQSAAQEIIAVAETSVGLAEKAGGLLNQMVPSIRKTAD 483 Query: 430 LVQDIVQSSEQQSTAVSQISTAASQLAQVIQQNSLSSEELAVTSEELNRRALLLQTAVGF 489 LVQ+I +S +QST + QI+ A Q+AQ Q + +SEEL+ TSEE++ +A+ LQ + F Sbjct: 484 LVQEIAAASHEQSTGLEQINNAVHQMAQTTQMTASASEELSATSEEMSAQAIHLQDLMRF 543 Query: 490 FQCQLARGMQAR 501 FQ R R Sbjct: 544 FQVGEPRKNAPR 555 >gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 535 Score = 198 bits (504), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 174/512 (33%), Positives = 268/512 (52%), Gaps = 27/512 (5%) Query: 1 LSVLILLALLGLVATAAVSLRQIENVYTAANYAQVNTVPSVTTLDDAQAAFNAIQGRIYQ 60 L +L L +LGL + + + + Q V AA+Y+ VNTVPS+ L DA A I+ I++ Sbjct: 7 LHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGIRVGIWK 66 Query: 61 YLVNPDPRDRSRLIKEMDVQRARMQRHLLTYANAYITDETDGAMLADDRAHIAAFEEVVR 120 Y DP R+RL K M Q R+ YA I+D+TD +L DR A +E+ Sbjct: 67 YAAATDPVSRTRLEKAMAEQHDRVLAAFDRYAREDISDDTDRKLLEADRQAFADYEKQRA 126 Query: 121 RLLAVAEEDRVEDSLGLSVLEFRAALDTVQASFARHRSYNVALGDASALTAREIIGQALE 180 +L + E R E++L D + A+ A HR Y LG TA I+ + Sbjct: 127 VVLRIGAESRNEEALRAIEQLVVPPGDKLIAALAEHRKYKEQLGRQGVETAEGILQSSTY 186 Query: 181 TVALVAGGVLLI--LLGAGWLLTRTLLRQMGGEPGDVVTVMQRLSDGDLVQTITLKPGCR 238 LV+ LL+ ++GA LL + R + D + V ++ GDL + K + Sbjct: 187 QAILVS---LLVAAIVGATSLL---ISRGISVSLDDAIRVANAIAHGDLSSKV--KMTGK 238 Query: 239 DS---MMHAIQKMMTRLAQVVGEVRGNADGLVSTAEEVSATAQSLSRASTEQANGVEQTS 295 D ++HAI +M L +VG VR + + S + E+++ LS + QA +E+T+ Sbjct: 239 DEVGQLLHAIGEMTASLVNIVGNVRQSVAAIESASSEIASGNLDLSARTEAQAGSIEETA 298 Query: 296 EALAQIHALLQSTAQNARLTDQIAAAAALEARQCAETVKQMVAAIQ-------QIAGKIA 348 A+ Q+ A ++ A NA D +A++A+ A + + V+++V + +I I+ Sbjct: 299 SAMEQLTATVKQNADNAHQADTLASSASSVASEGGKVVQEVVHTMDAINSSSLKIVDIIS 358 Query: 349 VIDDIAYQTNLLALNATIEAAHAGEHGSGFAVVAAEVRRLAERSQSAAQEIDEVARANVT 408 VID IA+QTN+LALNA +EAA AGE G GFAVVA+EVR LA+RS SAA+EI E+ +V Sbjct: 359 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVE 418 Query: 409 -------LACAAERQLSDMVPTITRTSVLVQDIVQSSEQQSTAVSQISTAASQLAQVIQQ 461 L A + +V ++ R S +V+DI +S +QS ++Q++ A + Q QQ Sbjct: 419 KVQSGSRLVMQAGNTMEQVVTSVQRVSDIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQ 478 Query: 462 NSLSSEELAVTSEELNRRALLLQTAVGFFQCQ 493 N+ EE A S+ L +A L AV F+ + Sbjct: 479 NAALVEEAAAASQSLREQAKGLAQAVSVFRLE 510 >gi|300311931|ref|YP_003776023.1| methyl-accepting chemotaxis II protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 195 bits (495), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 121/297 (40%), Positives = 192/297 (64%), Gaps = 2/297 (0%) Query: 198 WLLTRTLLRQMGGEPGDVVTVMQRLSDGDLVQTITLKPGCRDSMMHAIQKMMTRLAQVVG 257 W++ + R++GGEP ++++++DG+L + L+ R S+++A+ M RL +++ Sbjct: 233 WIIA-GVRRELGGEPAYAADIVRQIADGNLCVEVNLRSNDRSSLLYAMHTMRARLTEIMR 291 Query: 258 EVRGNADGLVSTAEEVSATAQSLSRASTEQANGVEQTSEALAQIHALLQSTAQNARLTDQ 317 V + L ++ +++ATA +L++AS+EQA VE+ A+A ++ +Q T ++AR T+Q Sbjct: 292 NVTLSTQALGVSSRQLNATAHALAQASSEQAAAVEEVHGAIASMNQAIQQTGEHARRTNQ 351 Query: 318 IAAAAALEARQCAETVKQMVAAIQQIAGKIAVIDDIAYQTNLLALNATIEAAHAGEHGSG 377 IA AAA +A + V+ V A++ IA ++ VIDDIAYQTNLLALNA IEAA AGEHG G Sbjct: 352 IAVAAASDADKSGAAVQTTVDAMRGIARQVGVIDDIAYQTNLLALNAAIEAARAGEHGRG 411 Query: 378 FAVVAAEVRRLAERSQSAAQEIDEVARANVTLA-CAAERQLSDMVPTITRTSVLVQDIVQ 436 F+VVAAEVR+LAERSQS+A EI +A+ +V LA ++R + + I + S + I Sbjct: 412 FSVVAAEVRKLAERSQSSAHEISVIAQQSVELAEQTSQRLVEGTLQGIRQASQQINQITL 471 Query: 437 SSEQQSTAVSQISTAASQLAQVIQQNSLSSEELAVTSEELNRRALLLQTAVGFFQCQ 493 SS Q V++I A +QL Q+N+ +SEELA+T+E + RA L + +F+ + Sbjct: 472 SSRMQEEGVAEIGVAVTQLNDTTQRNAAASEELAITAEAMAERARELNAQLAYFRLE 528 >gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 192 bits (489), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 158/511 (30%), Positives = 270/511 (52%), Gaps = 27/511 (5%) Query: 1 LSVLILLALLGLVATAAVSLRQIENVYTAANYAQVNTVPSVTTLDDAQAAFNAIQGRIYQ 60 L +LI+ +LG++ A V L Q + ++A Y+ +T+PSV L+ A A I+ I + Sbjct: 37 LYLLIVAVVLGVIMQAVVGLYQSHRIASSAGYSATDTIPSVLALNGATDAIYGIRLNIAR 96 Query: 61 YLVNPDPRDRSRLIKEMDVQRARMQRHLLTYANAYITDETDGAMLADDRAHIAAFEEVVR 120 Y++ P R+++ + M + Q + I+D+TD ML DR A +E+ Sbjct: 97 YVIEPTAREKT--VANMAALHQKAQDFFDQFERCCISDDTDRRMLEADRRFFAEYEKHRM 154 Query: 121 RLLAVAEEDRVEDSLGLSVLEFRAALDT-VQASFARHRSYNVALGDASALTAREIIGQAL 179 ++ + + ED+L +++ E A + A H++YN LG A A E + ++ Sbjct: 155 AMVDLGSAGK-EDALRVAMEEKMAPTGQRLMAVLNEHKNYNEQLGRQGAEAADEHLQLSI 213 Query: 180 ETVALVAGGVLLILLGAGWLLTRTLLRQMGGEPGDVVTVMQRLSDGDLVQTITLKPGCRD 239 + + A ++++++ A LL L+R + + + GDL I L+ RD Sbjct: 214 -YLTIAAATLVIVMVSALSLL---LVRNIRHSLHAATELADTIGRGDLSSQIVLRG--RD 267 Query: 240 S---MMHAIQKMMTRLAQVVGEVRGNADGLVSTAEEVSATAQSLSRASTEQANGVEQTSE 296 +M ++Q+M L Q++G+V +A + + E+++ LS + QA +E+T Sbjct: 268 EVTQLMLSLQRMTGSLVQILGKVNDSAATIETATREIASGNLDLSSRTEAQAGSLEETVS 327 Query: 297 ALAQIHALLQSTAQNARLTDQIAAAAALEARQCAETVKQMVAAIQ-------QIAGKIAV 349 A+ Q+ ++ A NAR D++A +A+ A Q + V +++ ++ +I I+V Sbjct: 328 AMGQLTTTVKQNADNARQADELAQSASGVAGQAGKVVDEVILTMEAINASSLKIVDIISV 387 Query: 350 IDDIAYQTNLLALNATIEAAHAGEHGSGFAVVAAEVRRLAERSQSAAQEIDEVARANVT- 408 ID IA+QTN+LALNA +EAA AGE G GFAVVA+EVR LA+RS SAA+EI E+ +V Sbjct: 388 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVNK 447 Query: 409 ------LACAAERQLSDMVPTITRTSVLVQDIVQSSEQQSTAVSQISTAASQLAQVIQQN 462 L A + ++V ++ R S +V+DI +S +QS ++Q++ A + Q QQN Sbjct: 448 VQSGSRLVLQAGSTMEEVVTSVRRVSGIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQN 507 Query: 463 SLSSEELAVTSEELNRRALLLQTAVGFFQCQ 493 + EE A S+ L +A L AV F+ + Sbjct: 508 AALVEEAAAASQSLREQAKGLAQAVSVFRLE 538 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.127 0.332 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,074,067 Number of extensions: 34903 Number of successful extensions: 614 Number of sequences better than 1.0e-08: 68 Number of HSP's gapped: 282 Number of HSP's successfully gapped: 69 Length of query: 503 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 414 Effective length of database: 1,198,455 Effective search space: 496160370 Effective search space used: 496160370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)