BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0453.p (554 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [... 1135 0.0 gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [... 569 e-163 >gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 554 Score = 1135 bits (2936), Expect = 0.0, Method: Compositional matrix adjust. Identities = 549/554 (99%), Positives = 551/554 (99%) Query: 1 MHTTALTDTAAFKALQHHHAKAVTWHMRELFAQQPDRFERMHVRAAGLLLDYSKNRIESE 60 MHTTALTDTAAFKALQHHHAKAVTWHMRELFAQQPDRFERMHVRAAGLLLDYSKNRIESE Sbjct: 1 MHTTALTDTAAFKALQHHHAKAVTWHMRELFAQQPDRFERMHVRAAGLLLDYSKNRIESE 60 Query: 61 TLALLMRLARERGVEALREAMFRGEKINFTEHRAVLHTALRAPAQACDAQVDGQPVSQEV 120 TLALLM LARERGVEALREAMFRGEKINFTEHRAVLHTALRAPAQACDAQVDGQPVSQEV Sbjct: 61 TLALLMSLARERGVEALREAMFRGEKINFTEHRAVLHTALRAPAQACDAQVDGQPVSQEV 120 Query: 121 HAVLDHIRDFSERVRSGAWRGYTGREITDIVNIGIGGSFLGPQMVCTALRNYRHARLTTH 180 HAVLDHIRDFSERVRSGAWRGYTGREITDIVNIGIGGSFLGPQMVCTALRNYRHARLTTH Sbjct: 121 HAVLDHIRDFSERVRSGAWRGYTGREITDIVNIGIGGSFLGPQMVCTALRNYRHARLTTH 180 Query: 181 FVSNVDGHDLDEVLSRVDPETTLFIVASKTFTTQETMLNAKSARAWFLRHDQQADLARHF 240 FVSNVDGHDLDEVLSRVDPETTLFIVASKTFTTQETMLNAKSARAWFLRH QQADLARHF Sbjct: 181 FVSNVDGHDLDEVLSRVDPETTLFIVASKTFTTQETMLNAKSARAWFLRHGQQADLARHF 240 Query: 241 VAVSTNAAEVEAFGIDPDNMFPFWDWVGGRYSVWSAIGLPIALLIGFEHFSAFLAGAHAM 300 VAVSTNAAEVE+FGIDPDNMFPFWDWVGGRYSVWSAIGLPIALLIGFEHFSAFLAGAHAM Sbjct: 241 VAVSTNAAEVESFGIDPDNMFPFWDWVGGRYSVWSAIGLPIALLIGFEHFSAFLAGAHAM 300 Query: 301 DRHFRDAPLEANMPVIQALIGIWNRNFFNSESLSVAPYHQWLRYFPAYLQQLDMESNGKR 360 DRHFRDAPLEANMPVIQALIGIWNRNFFNSESLSVAPYHQWLRYFPAYLQQLDMESNGKR Sbjct: 301 DRHFRDAPLEANMPVIQALIGIWNRNFFNSESLSVAPYHQWLRYFPAYLQQLDMESNGKR 360 Query: 361 ITRDGTPVGVATCPFIWGDVGTNGQHAFFQLLHQGTDVTPIDFIAVLKPSHGIDEHQDVL 420 ITRDGTPVGVATCPFIWGDVGTNGQHAFFQLLHQGT+VTPIDFIAVLKPSHGIDEHQDVL Sbjct: 361 ITRDGTPVGVATCPFIWGDVGTNGQHAFFQLLHQGTNVTPIDFIAVLKPSHGIDEHQDVL 420 Query: 421 LANCFAQSEAFMRGKQADEVRADLERQGVDAAELAALLPHKTFDGNRPSNTLLLPELSPH 480 LANCFAQSEAFMRGKQADEVRADLERQGVDAAELAALLPHKTFDGNRPSNTLLLPELSPH Sbjct: 421 LANCFAQSEAFMRGKQADEVRADLERQGVDAAELAALLPHKTFDGNRPSNTLLLPELSPH 480 Query: 481 ALGAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTIHGELRSEPNPVLHDASTNAL 540 ALGAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTIHGELRSEPNP LHDASTNAL Sbjct: 481 ALGAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTIHGELRSEPNPALHDASTNAL 540 Query: 541 IALARQARQADQQH 554 IALARQARQADQQH Sbjct: 541 IALARQARQADQQH 554 >gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 570 Score = 569 bits (1466), Expect = e-163, Method: Compositional matrix adjust. Identities = 291/550 (52%), Positives = 368/550 (66%), Gaps = 11/550 (2%) Query: 5 ALTDTAAFKALQHHHA-KAVTWHMRELFAQQPDRFERMHVRAAGLLLDYSKNRIESETLA 63 +LT +A+++LQ H+ +A + + LFA R E + A G+ LDYSKN + SET+A Sbjct: 21 SLTGKSAWRSLQEHYRDRARHFQLARLFAADDKRGESLTAEAVGIYLDYSKNLVTSETMA 80 Query: 64 LLMRLARERGVEALREAMFRGEKINFTEHRAVLHTALRAPAQACDAQVDGQPVSQEVHAV 123 LLM+LAR+ G+ EAMFRG++IN TE+R+VLHTALR PA VDGQ V EV V Sbjct: 81 LLMQLARQCGLPDRIEAMFRGDRINLTENRSVLHTALRKPASE-RLVVDGQDVVAEVQQV 139 Query: 124 LDHIRDFSERVRSGAWRGYTGREITDIVNIGIGGSFLGPQMVCTALRNYRHARLTTHFVS 183 L + F+ +VRSG W G+TG I +VNIGIGGS LGP M ALR Y H LT HFVS Sbjct: 140 LRRMEGFARQVRSGQWLGHTGLPIRHVVNIGIGGSDLGPMMAYEALRPYSHDDLTLHFVS 199 Query: 184 NVDGHDLDEVLSRVDPETTLFIVASKTFTTQETMLNAKSARAWFLRH-DQQADLARHFVA 242 +VDG + + + + E TLFIV SKTFTT ET+ NA +ARAW ++ + +ARHFV Sbjct: 200 DVDGAAMLDTVRGLPAEQTLFIVCSKTFTTLETITNATAARAWLVQQLGAEEAVARHFVG 259 Query: 243 VSTNAAEVEAFGIDPDNMFPFWDWVGGRYSVWSAIGLPIALLIGFEHFSAFLAGAHAMDR 302 V+TN E + FGID DNMF FWDWVGGRYS+ SAIGL + IG EHF A L G HAMD Sbjct: 260 VTTNIEEAQRFGIDADNMFGFWDWVGGRYSMTSAIGLSTMVAIGPEHFRAMLEGFHAMDE 319 Query: 303 HFRDAPLEANMPVIQALIGIWNRNFFNSESLSVAPYHQWLRYFPAYLQQLDMESNGKRIT 362 HFR APLE+N+PVI ++ +W NFF + S +V PY Q L+ PAYLQQL MESNGK IT Sbjct: 320 HFRSAPLESNLPVILGMLTVWYTNFFGAASQAVLPYSQHLKRLPAYLQQLTMESNGKHIT 379 Query: 363 RDGTPVGVATCPFIWGDVGTNGQHAFFQLLHQGTDVTPIDFIAVLKPSHGIDEHQDVLLA 422 +G PV T P WGD G NGQH+F+Q+LHQGT + P DFI + +P + + ++L+A Sbjct: 380 VNGEPVDYQTSPVYWGDNGINGQHSFYQMLHQGTQLVPCDFIGLCQPQQALGQQHELLMA 439 Query: 423 NCFAQSEAFMRGKQADEVRADLERQGVDAAELAALLPHKTFDGNRPSNTLLLPELSPHAL 482 N FAQSEA GK AD+VR + AL+PH+ F+GNRPSN++LL +L+P++L Sbjct: 440 NMFAQSEALAFGKDADQVRQE--------GTPEALVPHRVFEGNRPSNSMLLQQLTPYSL 491 Query: 483 GAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTIHGELRSEPNPVLHDASTNALIA 542 G ++ALYEH F QG +W I+SFDQWGVELGK LAK EL P+ HD+STNALI Sbjct: 492 GTLVALYEHSVFTQGVVWNIDSFDQWGVELGKQLAKRTARELIDHQQPLHHDSSTNALIR 551 Query: 543 LARQARQADQ 552 R A Q Sbjct: 552 RYRACSTATQ 561 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,761,943 Number of extensions: 77232 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 223 Number of HSP's successfully gapped: 2 Length of query: 554 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 464 Effective length of database: 1,193,720 Effective search space: 553886080 Effective search space used: 553886080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)