BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0447.p
         (248 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subun...   510   e-146
gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate ...    89   3e-19
gi|300309646|ref|YP_003773738.1| transketolase C-terminal subuni...    70   2e-13

>gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subunit
           [Herbaspirillum seropedicae SmR1]
          Length = 334

 Score =  510 bits (1313), Expect = e-146,   Method: Compositional matrix adjust.
 Identities = 246/248 (99%), Positives = 247/248 (99%)

Query: 1   MAKEGLVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCALPGLTTGYGPSHQATEDLA 60
           MAKEGLVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCALPGLTTGYGPSHQATEDLA
Sbjct: 87  MAKEGLVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCALPGLTTGYGPSHQATEDLA 146

Query: 61  MMRAIPGMTIVDPCDALDLEQAVPQIAAHQGPVYMRLARGNVPLVLDEYDYRFELGRAKE 120
           MMRAIPGMTIVDPCDALDLEQAVPQIAAHQGPVYMRLARGNVPLVLDEYDYRFELGRAKE
Sbjct: 147 MMRAIPGMTIVDPCDALDLEQAVPQIAAHQGPVYMRLARGNVPLVLDEYDYRFELGRAKE 206

Query: 121 IRDGNDVLIISSGFMTMRALEVAKALEADKLSVAVLHCPTIKPFDEATVVAQVKKKNRLV 180
           IRDGNDVLIISSGFMTMRALEVAKALEADK+SVAVLHCPTIKPFDEATVVAQVKKKNRLV
Sbjct: 207 IRDGNDVLIISSGFMTMRALEVAKALEADKVSVAVLHCPTIKPFDEATVVAQVKKKNRLV 266

Query: 181 VVAENHSVVGGLGEIVATTLMRHRVQPAGFHLAGLPDAFLDAGALPTLHDRYGISCEVLG 240
           VVAENHSVVGGLGEIVATTLMRHRVQPAGF LAGLPDAFLDAGALPTLHDRYGISCEVLG
Sbjct: 267 VVAENHSVVGGLGEIVATTLMRHRVQPAGFQLAGLPDAFLDAGALPTLHDRYGISCEVLG 326

Query: 241 RRIKEWLK 248
           RRIKEWLK
Sbjct: 327 RRIKEWLK 334


>gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate
           synthase [Herbaspirillum seropedicae SmR1]
          Length = 621

 Score = 89.4 bits (220), Expect = 3e-19,   Method: Compositional matrix adjust.
 Identities = 71/246 (28%), Positives = 111/246 (45%), Gaps = 9/246 (3%)

Query: 1   MAKEGLVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCALPGLTTGYGPSHQATEDLA 60
           MA EG+ P    Y+ F  +RAYD +   +A +NL+V       GL    G +H    DLA
Sbjct: 370 MACEGMKPVVAIYSTF-LQRAYDQLIHDVALQNLDVTFALDRAGLVGADGATHAGNYDLA 428

Query: 61  MMRAIPGMTIVDPCDALDLEQAVPQIAAHQGPVYMRLARG-NVPLVLDEYDYRFELGRAK 119
            +R IP M ++   D  +  + +     + GP  +R  RG  +   +D+      LGR +
Sbjct: 429 FLRCIPNMVVMAASDENECRKMLSTAYQYPGPASVRYPRGAGIGAAVDQTLETLPLGRGE 488

Query: 120 EIRDGNDVLIISSGFMTMRALEVAKALEADKLSVAVLHCPTIKPFDEATVVAQVKKKNRL 179
             R G +V I++ G +   +L        DKL+  V +   IKP D   +V Q+   +  
Sbjct: 489 IRRQGKNVAILAFGSLLAPSLGA-----GDKLNATVANMRFIKPLD-VELVKQLAASHDA 542

Query: 180 VVVAENHSVVGGLGEIVATTLMRHRVQPAGFHLAGLPDAFLDAGALPTLHDRYGISCEVL 239
           +V  E   ++GG G  V+  L    +     HL GLPD F+D G    L  + G+    +
Sbjct: 543 LVTVEEGCIMGGAGAAVSEALAAAGINKPILHL-GLPDRFIDHGDAAQLLAQCGLDANGI 601

Query: 240 GRRIKE 245
              I +
Sbjct: 602 ADSIAQ 607


>gi|300309646|ref|YP_003773738.1| transketolase C-terminal subunit
           [Herbaspirillum seropedicae SmR1]
          Length = 321

 Score = 69.7 bits (169), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 59/253 (23%), Positives = 112/253 (44%), Gaps = 10/253 (3%)

Query: 1   MAKEGLVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCALPGLTTGY-----GPSHQA 55
           +A EG   FA   A F T R Y+ +   +A  +    +   L G+  GY     GP+HQ 
Sbjct: 66  LALEGYKVFAYAIAPFITMRCYEQVRVSLALLSEVRPMNVNLIGVGAGYSYVVSGPTHQC 125

Query: 56  TEDLAMMRAIPGMTIVDPCDALDLEQAVPQIAAHQGPVYMRLARGNVPLVLDEYDYRFEL 115
            ED+ +MRA+P   ++   D +         +   GP Y+R     +P++    D   +L
Sbjct: 126 YEDITLMRALPNFQVLSAADHVTAAAFFDTCSNSMGPKYLRFDAQVLPVIYG--DGAPDL 183

Query: 116 GRAKEI-RDGNDVLIISSGFMTMRALEVAKALEADKLSVAVLHCPTIKPFDEATVVAQVK 174
            +   I R G  + ++++G+M   AL+ A+ L A+ + V V+    +  F    +   ++
Sbjct: 184 AQGFHIHRRGGKIGLVATGYMLHTALQAAEVLAAEGIEVGVIDLFDLARFSADALQETLQ 243

Query: 175 KKNRLVVVAENHSVVGGLGEIVATTLMRHRVQPAGFHLAGLPDAF-LDAGALPTLHDRYG 233
               +  + E     GG+  ++   + R  +Q    ++ G+   +  + G    LH+  G
Sbjct: 244 SYAGIATLEEGFRGRGGMDSMLFEFIARRGLQTRMLNI-GVDGVYRFELGTRTELHEEVG 302

Query: 234 ISCEVLGRRIKEW 246
           I  + +   I+E+
Sbjct: 303 IGPQAVLTSIREF 315


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.323    0.138    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 788,448
Number of extensions: 32553
Number of successful extensions: 128
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 125
Number of HSP's successfully gapped: 3
Length of query: 248
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 165
Effective length of database: 1,226,865
Effective search space: 202432725
Effective search space used: 202432725
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 129 (54.3 bits)