BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0444.p (281 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [He... 575 e-165 gi|300309645|ref|YP_003773737.1| transketolase N-terminal subuni... 147 7e-37 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 104 7e-24 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 98 6e-22 >gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [Herbaspirillum seropedicae SmR1] Length = 281 Score = 575 bits (1483), Expect = e-165, Method: Compositional matrix adjust. Identities = 280/281 (99%), Positives = 281/281 (100%) Query: 1 MSDNLANLRASAYRIRRNAVRMGEVQGQGYIGQALGWADVLAVAYKHAMSLRPDDPQWSG 60 MSDNLANLRASAYRIRRNAVRMGEVQGQGYIGQALGWADVLAVAYKHAMSLRPDDPQWSG Sbjct: 1 MSDNLANLRASAYRIRRNAVRMGEVQGQGYIGQALGWADVLAVAYKHAMSLRPDDPQWSG 60 Query: 61 RDRFLLSHGHYAIALYAALIEAGIVPDEELDSYGSDDSRLPMSGMATYTPGMEISGGSLG 120 RDRFLLSHGHYAIALYAALIEAGIVPDEELDSYGSDDSRLPMSGMATYTPGMEISGGSLG Sbjct: 61 RDRFLLSHGHYAIALYAALIEAGIVPDEELDSYGSDDSRLPMSGMATYTPGMEISGGSLG 120 Query: 121 QGLAIGVGMALGLRYNNNPAFVYNSMSDGELDEGSTWEAAMGAAHHQMGNLICLVDINNQ 180 QGLAIGVGMALGLRYNNNPAFVYNSMSDGELDEGSTWEAAMGAAHHQ+GNLICLVDINNQ Sbjct: 121 QGLAIGVGMALGLRYNNNPAFVYNSMSDGELDEGSTWEAAMGAAHHQLGNLICLVDINNQ 180 Query: 181 QADGPSGKIMGFEPLADKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFDTL 240 QADGPSGKIMGFEPLADKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFDTL Sbjct: 181 QADGPSGKIMGFEPLADKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFDTL 240 Query: 241 MGKGVPFLEQREKNHFIRVDPPEWQQALSILDQSYQQGSQA 281 MGKGVPFLEQREKNHFIRVDPPEWQQALSILDQSYQQGSQA Sbjct: 241 MGKGVPFLEQREKNHFIRVDPPEWQQALSILDQSYQQGSQA 281 >gi|300309645|ref|YP_003773737.1| transketolase N-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 272 Score = 147 bits (372), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 93/263 (35%), Positives = 135/263 (51%), Gaps = 4/263 (1%) Query: 1 MSDNLANLRASAYRIRRNAVRMGEVQGQGYIGQALGWADV-LAVAYKHAMSLRPDDPQWS 59 M ++ +LRA A+ + R + + + + +L D+ +A+ Y + P DP+ + Sbjct: 1 MKNDFVDLRAKAHWVWRETLAIHRRAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDA 60 Query: 60 GRDRFLLSHGHYAIALYAALIEAGIVPDEELDSYGSDDSRLPMSGMA-TYTPGMEISGGS 118 GRDR ++S GH +I +Y L + G P EEL S L G+ PG E GS Sbjct: 61 GRDRCVISKGHGSICMYPILADLGYFPMEELQHVCEAGSFL--GGIPDPVIPGYETVNGS 118 Query: 119 LGQGLAIGVGMALGLRYNNNPAFVYNSMSDGELDEGSTWEAAMGAAHHQMGNLICLVDIN 178 LG GL + GMALGL+ + + V+ DGEL EG+ WEA M A+ H++ NL +VD N Sbjct: 119 LGHGLGVATGMALGLKRSGSDRSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDN 178 Query: 179 NQQADGPSGKIMGFEPLADKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFD 238 G + I+ LA + +AFGW VDG+D+ AV A + KP+ I+ Sbjct: 179 RISMLGYTEDIVSHGELATRLSAFGWDCMEVDGHDVLAVQAALLQQKAHAAGKPKAIIAR 238 Query: 239 TLMGKGVPFLEQREKNHFIRVDP 261 TL G GVP LE +H I P Sbjct: 239 TLKGHGVPGLENAPLSHIINPKP 261 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 104 bits (260), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 79/255 (30%), Positives = 125/255 (49%), Gaps = 16/255 (6%) Query: 28 QGYIGQALGWADVLAVAYKHAMSLRPDDPQWSGRDRFLLSHGHYAIALYAALIEAGI--V 85 +G+ G LG A++ Y + P P W RDR +LS+GH ++ LY L G + Sbjct: 26 EGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRDRVVLSNGHGSMLLYCLLYLTGYEHI 85 Query: 86 PDEELDSYGSDDSRLPMSGMATYTP--GMEISGGSLGQGLAIGVGMALGLRYNNNP---- 139 E++ S+ S +G Y P G+E++ G LGQG+A GMA+ Y N Sbjct: 86 TLEQVRSFREFGSHC--AGHPEYAPELGIEVTTGPLGQGIANAFGMAVAEAYLNARFGDD 143 Query: 140 ---AFVYNSMSDGELDEGSTWEAAMGAAHHQMGNLICLVDINNQQADGPSGKIMGFEPLA 196 Y + DG L EG E A H ++ L L D +NQ D ++ E +A Sbjct: 144 IVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTLLWD-DNQITDDGKTELSISENVA 202 Query: 197 DKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFDTLMGKGVPFLEQREKNHF 256 +++ GW+V VDG+D+ AV +A D A+ +P +I T++G+G+P ++ + H Sbjct: 203 ERFRVAGWHVVEVDGHDIQAVSRALDAAQG--SDRPSMIACRTVIGRGIPRVQGQRGGHS 260 Query: 257 IRVDPPEWQQALSIL 271 R+ + Q A +L Sbjct: 261 ARLYEDDAQAARELL 275 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 98.2 bits (243), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 84/257 (32%), Positives = 123/257 (47%), Gaps = 24/257 (9%) Query: 18 NAVR---MGEVQ--GQGYIGQALGWADVLAVAYKHAMSLRPDDPQWSGRDRFLLSHGHYA 72 NA+R M VQ G+ G +G A++ + P +P W+ RDRF+LS+GH + Sbjct: 12 NAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPANPHWANRDRFVLSNGHGS 71 Query: 73 IALYAALIEAGI-VPDEELDSYGSDDSRLPMSGMATYTPGMEISGGSLGQGLAIGVGMAL 131 + YA L G + E++ S+ S+ TPG+E + G LGQG+ VGMAL Sbjct: 72 MLQYALLHLTGYDLSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLGQGITNAVGMAL 131 Query: 132 GLRY----NNNPA------FVYNSMSDGELDEGSTWEAAMGAAHHQMGNLICLVDINNQQ 181 + N P + Y + DG L EG + EA A + LI L D N Sbjct: 132 AEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSKLIVLYDDNGIS 191 Query: 182 ADGPSGKIMGF--EPLADKWAAFGW-YVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFD 238 D G + G+ + ++ A+GW + VDG+ + AV A A+ L D KP +I Sbjct: 192 ID---GHVEGWFKDDTPKRFEAYGWNVIPAVDGHSVEAVSAAIHQAK-LAD-KPTLICCR 246 Query: 239 TLMGKGVPFLEQREKNH 255 T++GKG P L +K H Sbjct: 247 TVIGKGSPNLAGTDKVH 263 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 948,575 Number of extensions: 41424 Number of successful extensions: 125 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 4 Length of query: 281 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 197 Effective length of database: 1,222,130 Effective search space: 240759610 Effective search space used: 240759610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)