BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0440.p (295 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310443|ref|YP_003774535.1| LysR family transcription regul... 591 e-170 gi|300309845|ref|YP_003773937.1| LysR family transcription regul... 318 3e-88 gi|300313122|ref|YP_003777214.1| transcription regulator protein... 149 4e-37 gi|300314051|ref|YP_003778143.1| transcription regulator protein... 137 9e-34 gi|300313964|ref|YP_003778056.1| LysR family transcription regul... 134 1e-32 >gi|300310443|ref|YP_003774535.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 295 Score = 591 bits (1524), Expect = e-170, Method: Compositional matrix adjust. Identities = 294/295 (99%), Positives = 295/295 (100%) Query: 1 MAVLPPLPNLQAFEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVR 60 MAVLPPLPNLQAFEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVR Sbjct: 1 MAVLPPLPNLQAFEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVR 60 Query: 61 ITTAGEAYLARIQGAISAITAASMDVRQGVNNTLYIHSAPSIASLWLMARLNDFARSYPE 120 ITTAGEAYLARIQGAISAITAASMDVRQGV+NTLYIHSAPSIASLWLMARLNDFARSYPE Sbjct: 61 ITTAGEAYLARIQGAISAITAASMDVRQGVSNTLYIHSAPSIASLWLMARLNDFARSYPE 120 Query: 121 ITFNLSAAHSQSNFELGEVDIDIRYGIPNWPGLVVEPLFEERIVPLASPAFIREYRLRKP 180 ITFNLSAAHSQSNFELGEVDIDIRYGIPNWPGLVVEPLFEERIVPLASPAFIREYRLRKP Sbjct: 121 ITFNLSAAHSQSNFELGEVDIDIRYGIPNWPGLVVEPLFEERIVPLASPAFIREYRLRKP 180 Query: 181 ADLLGVKLIRSNVSVVQWADWFGEFADVRAPEQFAVRFDRAQMSLDAATQGLGVALESAT 240 ADLLGVKLIRSNVSVVQWADWFGEFADVRAPEQFAVRFDRAQMSLDAATQGLGVALESAT Sbjct: 181 ADLLGVKLIRSNVSVVQWADWFGEFADVRAPEQFAVRFDRAQMSLDAATQGLGVALESAT 240 Query: 241 VAARHLEEKKLKPIFGLERAIKVKAHFAVYPAHHARRPAVAAFLEWLREQADGQG 295 VAARHLEEKKLKPIFGLERAIKVKAHFAVYPAHHARRPAVAAFLEWLREQADGQG Sbjct: 241 VAARHLEEKKLKPIFGLERAIKVKAHFAVYPAHHARRPAVAAFLEWLREQADGQG 295 >gi|300309845|ref|YP_003773937.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 318 bits (816), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 152/289 (52%), Positives = 208/289 (71%) Query: 3 VLPPLPNLQAFEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVRIT 62 ++PPL +L AFEA+ARRKSF LAAAELHLT SA+SHQVARLE LG+RLFERS GV +T Sbjct: 1 MIPPLTSLMAFEAIARRKSFNLAAAELHLTPSAISHQVARLEKLLGLRLFERSTKGVELT 60 Query: 63 TAGEAYLARIQGAISAITAASMDVRQGVNNTLYIHSAPSIASLWLMARLNDFARSYPEIT 122 AG++YL R+ GA+ AI +A+ D+R GV N+L IHS+PS ASLWLM R+ F + +P+I Sbjct: 61 PAGQSYLNRVAGALGAINSATDDLRHGVENSLQIHSSPSFASLWLMPRIGRFTQRHPDIA 120 Query: 123 FNLSAAHSQSNFELGEVDIDIRYGIPNWPGLVVEPLFEERIVPLASPAFIREYRLRKPAD 182 LSA+ S+FE+G+VD+DIR G+P+WP L +E + ER++P+ASP F+ +R+ P D Sbjct: 121 LGLSASPEISDFEIGQVDLDIRCGVPHWPDLEIEAVLSERVIPMASPDFLARHRIDTPDD 180 Query: 183 LLGVKLIRSNVSVVQWADWFGEFADVRAPEQFAVRFDRAQMSLDAATQGLGVALESATVA 242 L+ LI+S S++QW +WF +A PE+ A+RFDRA MS++AA QGLGV ES Sbjct: 181 LVLAPLIQSRGSLLQWPEWFARYAPDLRPERIALRFDRAMMSIEAAVQGLGVVFESDAFG 240 Query: 243 ARHLEEKKLKPIFGLERAIKVKAHFAVYPAHHARRPAVAAFLEWLREQA 291 A L +L+P+F A+++ H+AV PA + RP V FL+WLRE++ Sbjct: 241 AVALASGRLQPVFADGLALEITQHYAVCPARNKSRPEVMLFLQWLREES 289 >gi|300313122|ref|YP_003777214.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 296 Score = 149 bits (375), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 96/289 (33%), Positives = 145/289 (50%), Gaps = 7/289 (2%) Query: 4 LPPLPNLQAFEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVRITT 63 LPP+ L AFEA AR SFA+AA EL +T SA+SH++ LE ++G RLF R + ++ Sbjct: 5 LPPVHALSAFEAAARHNSFAIAAEELCITPSALSHRIRLLEEFVGERLFYRDGRSIGLSE 64 Query: 64 AGEAYLARIQGAISAITAASMDVRQ-GVNNTLYIHSAPSIASLWLMARLNDFARSYPEIT 122 G YL ++ A+ +T M R V + + P+ A LM R+ DF + +PEI Sbjct: 65 FGRRYLDVVRSALRTLTDFPMPHRNAAVQPKIKVMLPPTFARHLLMPRIADFTQRHPEIV 124 Query: 123 FNLSAAHSQSNFELGEVDIDIRYGIPNWPGLVVEPLFEERIVPLASPAFIREY-RLRKPA 181 L + + L E D++IR+G N+P ++ E LFEE +ASPA+++ ++ PA Sbjct: 125 VELYLSVPLYDLSLSESDVEIRFGAGNYPNIITEKLFEEPAFAVASPAYLKTLPPMKSPA 184 Query: 182 DLLGVKLIRSNVSVVQWADWF--GEFADVRAPEQFAVRFDRAQMSLDAATQGLGVALESA 239 DL L+RS + W WF D P +R D + L+A G GV L Sbjct: 185 DLKQATLLRSALE--PWQPWFEAAGLKDWVEPSA-GLRVDDLGLLLEAVRHGYGVGLTRQ 241 Query: 240 TVAARHLEEKKLKPIFGLERAIKVKAHFAVYPAHHARRPAVAAFLEWLR 288 A L ++ +F + A++ VY RP V F+ W++ Sbjct: 242 HFAEELLARGEIVELFDIRLRTPPHAYYVVYERAVRERPEVDTFISWMQ 290 >gi|300314051|ref|YP_003778143.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 306 Score = 137 bits (345), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 101/297 (34%), Positives = 157/297 (52%), Gaps = 10/297 (3%) Query: 1 MAVLPPLPNLQAFEAVARR---KSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAH 57 MA L LPNL A A + F+ AA E+H+T A+SHQV LE LG++LF R Sbjct: 1 MADLRKLPNLAALRAFDAAARYECFSRAAEEVHVTPGAISHQVRALEEDLGLQLFLRHGK 60 Query: 58 GVRITTAGEAYLARIQGAISAITAASMDVR-QGVNNTLYIHSAPSIASLWLMARLNDFAR 116 + IT G+ + A I+ ++S I A+ +R Q L I + PS AS WL RL F Sbjct: 61 RISITETGQRFAATIRKSLSEIALAAEHLRSQNRQQRLVISTTPSFASRWLAPRLWRFID 120 Query: 117 SYPEITFNLSAAHSQSNFELGEVDIDIRYGIPNWPGLVVEPLFEERIVPLASPAF--IRE 174 ++PE+ L +A ++ L +D+ +R+G N+ GLV E L ++ P+A+P + R Sbjct: 121 AHPELEVVLQSAAHLNDLRLDGIDVGLRFGRGNYSGLVSEKLSDDYYYPVAAPGYRGKRS 180 Query: 175 YRLRKPADLLGVKLIRSNVSVVQWADWFGEFADVRAPE-QFAVRFDRAQMSLDAATQGLG 233 +L +PADL L+R + S W W E A + PE + + + ++L A +G G Sbjct: 181 KKLARPADLAHCSLLRVD-SRESWQPWLAE-AGLELPEPTGGLLVEDSSLTLRQAAEGKG 238 Query: 234 VALESATVAARHLEEKKLKPIFGLERAIKVKAHFAVYPAHHARRPAVAAFLEWLREQ 290 VAL +A++ + +L +F + + AH+ V+ +P VAAF EW+ E+ Sbjct: 239 VALARHVIASQEISTGELVRLFDVVVPSE-DAHYFVHLPEALEKPGVAAFKEWIFEE 294 >gi|300313964|ref|YP_003778056.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 314 Score = 134 bits (336), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 103/314 (32%), Positives = 149/314 (47%), Gaps = 22/314 (7%) Query: 1 MAVLPPLPNLQAFEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVR 60 M LPPL L+AFEA AR SF AA ELH+T SAVS QV LE++L +LF R + Sbjct: 1 MNKLPPLTALRAFEATARLLSFTAAADELHVTQSAVSRQVRILEAHLEQKLFVRLTRRIE 60 Query: 61 ITTAGEAYLARIQGAISAITAASMDV---RQGVNNTLYIHSAPSIASLWLMARLNDFARS 117 +T AG AY +I+ A I AA + RQ +TL I P +ASLWL+ RL F R Sbjct: 61 LTRAGAAYFQQIRQAFERICAAGEQLHARRQ--RHTLTIGVLPGLASLWLVPRLELFWRE 118 Query: 118 YPEITFNLSAAHSQSNFELGEVDIDIRYG-IP---------------NWPGLVVEPLFEE 161 EI + A ++F +D+ I+ G +P +W L + LF + Sbjct: 119 NKEIDVRIVVATGAADFGRTTMDMSIQVGNLPGTTSQGLPLKKRMAVDWHDLRADLLFPD 178 Query: 162 RIVPLASPAFIREYRLRKPADLLGVKLIRSNVSVVQWADWFGEFADVRAPEQFAVRFDRA 221 +V L P R++ +R ADL +LI + + WA W + Q + + Sbjct: 179 ALVALCHPRLWRQHPVRSVADLSQHRLIHTAIRPDAWAHWLQQHGSRLGARQAGMECSQY 238 Query: 222 QMSLDAATQGLGVALESATVAARHLEEKKLKPIFGLERAIKVKAHFAVYPAHHARRPAVA 281 ++L AA G G+AL + +A + +L+ +FG ++ + P AV Sbjct: 239 PVALQAAMNGEGIALVPSVLAESFDPQHRLRRLFG-GPTPSAGQYYLLTPGDRYDDSAVR 297 Query: 282 AFLEWLREQADGQG 295 F +WL QA G Sbjct: 298 RFRQWLLMQAQAMG 311 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 869,834 Number of extensions: 34636 Number of successful extensions: 243 Number of sequences better than 1.0e-08: 52 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 52 Length of query: 295 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 210 Effective length of database: 1,217,395 Effective search space: 255652950 Effective search space used: 255652950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)