BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0437.p (850 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein... 1704 0.0 gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein... 74 4e-14 gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein... 73 9e-14 gi|300311857|ref|YP_003775949.1| integral membrane protein [Herb... 58 3e-09 >gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein component [Herbaspirillum seropedicae SmR1] Length = 850 Score = 1704 bits (4412), Expect = 0.0, Method: Compositional matrix adjust. Identities = 842/850 (99%), Positives = 843/850 (99%) Query: 1 MFKKIWFQLHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQVRQQATLTPQQ 60 MFKKIWFQLHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQVRQQATLTPQQ Sbjct: 1 MFKKIWFQLHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQVRQQATLTPQQ 60 Query: 61 VLEKLRQEEPQRRITTMTLSAEPGDSVRVVFAPPPGERRGQARYVDPYTGALLPPVTGME 120 VLEKLRQEEPQRRITTMTLSAEPGDSVRVVFAPPPGERRGQARYVDPYTGALLPPVTGME Sbjct: 61 VLEKLRQEEPQRRITTMTLSAEPGDSVRVVFAPPPGERRGQARYVDPYTGALLPPVTGME 120 Query: 121 FFEFTERFHRWLLLPTEIGKIVLGTLALCLLMLALSGLYLRWPRRALSLRAWLRLDFQLQ 180 FFEFTERFHRWLLLPTEIGKIVLGTLALCLLMLALSGLYLRWPRRALSLRAWLRLDFQLQ Sbjct: 121 FFEFTERFHRWLLLPTEIGKIVLGTLALCLLMLALSGLYLRWPRRALSLRAWLRLDFQLQ 180 Query: 181 GRSLLWNLHSVIGTWSLVMYVIFATTGAYWALDWFKDGVNTLAGETRRAPMARGEGKKKD 240 GRSLLWNLHSVIGTWSLVMYVIFATTGAYWALDWFKDGVNTLAGETRRAPMARGEGKKKD Sbjct: 181 GRSLLWNLHSVIGTWSLVMYVIFATTGAYWALDWFKDGVNTLAGETRRAPMARGEGKKKD 240 Query: 241 GPNNQTPVALDLTQAWNTFLAKVPDYQSAQLRLPEQGTQPLQIFFLGHDAPHERARSRMV 300 GPNNQTPVALDLTQAWN FL K PDYQSAQLRLPEQGTQPLQIFFLGHDAPHERARSRMV Sbjct: 241 GPNNQTPVALDLTQAWNAFLTKAPDYQSAQLRLPEQGTQPLQIFFLGHDAPHERARSRMV 300 Query: 301 VLPQSGEIKQLERYEDKSTGGRFIAAIYPLHMGTYFGLPGRIIMAIASLMMPLFGITGWM 360 VLPQSGEIKQLERYEDKSTGGRFIAAIYPLHMGTYFGLPGRIIMAIASLMMPLFGITGWM Sbjct: 301 VLPQSGEIKQLERYEDKSTGGRFIAAIYPLHMGTYFGLPGRIIMAIASLMMPLFGITGWM 360 Query: 361 LYLDRRRKKAALRAERAALGEPQQQAGGQLLVAYASQTGQAERIALHTAARLRRSGVAVT 420 LYLDRRRKKAALRAERAALGEPQQQAGGQLLVAYASQTGQAERIALHTAARLRRSGVAVT Sbjct: 361 LYLDRRRKKAALRAERAALGEPQQQAGGQLLVAYASQTGQAERIALHTAARLRRSGVAVT 420 Query: 421 LQALAQTTAETLRHHPRLLLIASTFGEGDAPDSARGFERRLRQAAGQSLAHLHYAVLALG 480 LQALAQTTAETLRHHPRLLLIASTFGEGDAPDSARGFERRLRQAAGQSLAHLHYAVLALG Sbjct: 421 LQALAQTTAETLRHHPRLLLIASTFGEGDAPDSARGFERRLRQAAGQSLAHLHYAVLALG 480 Query: 481 DRHYTSFCGFGHTLDHGLRQGGAKPMFPLVEVDRMDAAALRKWESELASHGLDAAVSDLL 540 DRHYTSFCGFGHTLDHGLRQ GAKPMFPLVEVDRMDAAALRKWESELASHGLDAAVSDLL Sbjct: 481 DRHYTSFCGFGHTLDHGLRQCGAKPMFPLVEVDRMDAAALRKWESELASHGLDAAVSDLL 540 Query: 541 PTSSPTWSSWVLVERHHLNAGSQGEPLFHLVLQSADSSQPAPTWQAGALLEVMPRHAPDR 600 PTSSPTW SWVLVERHHLNAGSQGEPLFHLVLQSADSSQPAP WQAGALLEVMPRHAPDR Sbjct: 541 PTSSPTWPSWVLVERHHLNAGSQGEPLFHLVLQSADSSQPAPAWQAGALLEVMPRHAPDR 600 Query: 601 VALVLQRLGLDGATEVHCRGMRCSLADALACSVLPMQAAADAQTQAQQLQPLTARSYSVA 660 VALVLQRLGLDGATEVHCRGMRCSLADALACSVLPMQAAADAQT AQQLQPLTARSYSVA Sbjct: 601 VALVLQRLGLDGATEVHCRGMRCSLADALACSVLPMQAAADAQTLAQQLQPLTARSYSVA 660 Query: 661 SAMQDGTVQLLVRQQRHEDGLGLASGWLTEYAPLGARLQARLVDNAGFAPVSADTPAIFI 720 SAMQDGTVQLLVRQQRHEDGLGLASGWLTEYAPLGARLQARLVDNAGFAPVSADTPAIFI Sbjct: 661 SAMQDGTVQLLVRQQRHEDGLGLASGWLTEYAPLGARLQARLVDNAGFAPVSADTPAIFI 720 Query: 721 GNGSGMAGLRGHLRQRIHAGGTRNWLLFGERNERYDFLYREELQAWLQQGQLARLDLAFS 780 GNGSGMAGLRGHLRQRIHAGGTRNWLLFGERNERYDFLYREELQAWLQQGQLARLDLAFS Sbjct: 721 GNGSGMAGLRGHLRQRIHAGGTRNWLLFGERNERYDFLYREELQAWLQQGQLARLDLAFS 780 Query: 781 RDQEARVYVQDRLREAAGELRHWLDQGAVLYVCGSLDGMAAGVDAVLTSLLGETGLQDLI 840 RDQEAR+YVQDRLREAAGELRHWLDQGAVLYVCGSLDGMAAGVDAVLTSLLGETGLQDLI Sbjct: 781 RDQEARIYVQDRLREAAGELRHWLDQGAVLYVCGSLDGMAAGVDAVLTSLLGETGLQDLI 840 Query: 841 EQNRYRRDVY 850 EQNRYRRDVY Sbjct: 841 EQNRYRRDVY 850 >gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 416 Score = 74.3 bits (181), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 97/415 (23%), Positives = 174/415 (41%), Gaps = 49/415 (11%) Query: 9 LHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQVRQQ----ATLTPQQVLEK 64 LH + G+ A L G+TGA++++ E+ L+P + + Q A + ++ + Sbjct: 8 LHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSATQPGAGAPMPGLELARR 67 Query: 65 LRQEEPQRRITTMTLSAEPGDSVRVVFAPPPGERRGQARY-------VDPYT-------- 109 + Q EP+ R+T + AEPG ++ ++ P G+ VDP+T Sbjct: 68 VEQAEPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPFTAEIQGRRM 127 Query: 110 -GALLPPVTGMEFFEFTERFHRWLLLPT----EIGKIVLGTLALCLLMLALSGLYLRWPR 164 GA+ ++ F + H + LP E G +++G + + + L LYL +P Sbjct: 128 WGAI--SLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALYLSFP- 184 Query: 165 RALSLRAW---LRLDFQLQGRSLLWNLHSVIGTWSLVMYVIFATTGAYWALD--WFKDGV 219 + R+W L ++ G L ++LH G W + +I A T L+ + + Sbjct: 185 ---NWRSWRKSLAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQVMRPLL 241 Query: 220 NTLAGET-----RRAPMARGEGKKKDGPNNQTPVALDLTQAWNTFLAKVPD--YQSAQLR 272 TL+ T R P A E + + VAL QA L + + S Sbjct: 242 QTLSTLTPSPFDTRTPTAP-EKVAEPRITREQAVALGQAQARKMGLTEPVGGVFYSGMF- 299 Query: 273 LPEQGTQPLQIFFLGHD-APHERARSRMVVLPQSGEIKQLERYEDKSTGGRFIAAIYPLH 331 G + F G+D + + SG + + S G F+ A +PLH Sbjct: 300 ----GVYGVGYFQPGNDHGDIGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFLQAQFPLH 355 Query: 332 MGTYFGLPGRIIMAIASLMMPLFGITGWMLYLDRRRKKAALRAERAALGEPQQQA 386 G GLPGRI+++ ++ + +TG ++++ +R+ + R AA P ++ Sbjct: 356 SGRILGLPGRILISFMGALVAMLSVTGVIIWVRKRKARLQQRERTAAQKTPAREG 410 >gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 73.2 bits (178), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 101/421 (23%), Positives = 173/421 (41%), Gaps = 48/421 (11%) Query: 9 LHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQV----RQQATLTPQQVLEK 64 LH + G+ L+ +TG+LL F +E+ L+P + Q QA + P + EK Sbjct: 25 LHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQLQLAQPAPGQSAQAMMDPYLLREK 84 Query: 65 LRQEE-PQRRITTMTLSAEPGDSVRVVFAPPPGERRGQAR-------YVDPYTGAL---- 112 + Q PQ I+ + L A PG +V P G+ V+PYTG + Sbjct: 85 VAQAMGPQVHISQLVLHARPGRTVSFQADPATDPATGKPYRLGYDEILVNPYTGVVQGVR 144 Query: 113 ------LPPVTGMEFFEFTERFHRWLLLPTEIGKIVLGTLALCLLMLALSGLYLRWPRRA 166 L P + F + H L LP G ++LGT+ L + G+ L WPR Sbjct: 145 DRDKISLKPA---DLMPFLFKLHHSLALPRAPGALLLGTVGLLWTLDCFVGMILTWPRAR 201 Query: 167 LSLRAWLRLDFQLQGRSLLW----NLHSVIGTWSLVMYVIFATTGAYWALDWFKDGV--- 219 L W + + ++ R+ + +LH G W VM ++FA + L +D + Sbjct: 202 PFLVKW-KPAWLIKWRAGFYRVNLDLHRAFGLWCWVMLLLFAWSSVMLNL---RDQIYLP 257 Query: 220 ---NTLAGETRRAPMARGEGKKKDGPNNQTPVALDLTQAWNTFLAK----VPDYQSAQLR 272 L+ +T P + GP P A L + A+ V D++ +L Sbjct: 258 VMSRILSFDTSWRP-GPPLPQALSGPPQSWPEAHALARRTMDGFARQRGLVIDFEE-KLD 315 Query: 273 LPEQGTQPLQIFFLGHDAPHERARSRMVVLPQSGEIKQLERYEDKSTGGRFIAAIYPLHM 332 + + + D + + + + ++G+I G F + LHM Sbjct: 316 FDRRRGVYIYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGDRDGNTFSNWLGALHM 375 Query: 333 GTYFGLPGRIIMAIASLMMPLFGITGWMLYLDRRRKKAALRAERAALGEPQQQAGGQLLV 392 G FGLP RI++ + + L +TG +++ +K+AA R + + G+ ++ + V Sbjct: 376 GHVFGLPWRILLVLVGGAVSLLTVTGVVVWW---KKRAARRLQLRSPGDGRKPSASHSDV 432 Query: 393 A 393 A Sbjct: 433 A 433 >gi|300311857|ref|YP_003775949.1| integral membrane protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 57.8 bits (138), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 97/419 (23%), Positives = 163/419 (38%), Gaps = 93/419 (22%) Query: 7 FQLHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQVRQQATLTPQQVLEKLR 66 ++H++IG+ G ++ +TG L +I L Q+R A+ P Q L Sbjct: 34 LRMHFYIGLFVGPFILVAALTGTLYVLTPQIESHL----YATQLRN-ASQGPAQPLAAQV 88 Query: 67 QEE-----PQRRITTMTLSAEPGDSVRVVFAPPP-GERRGQARYVDPY------------ 108 Q PQ R+ + + PG + RV+F+ P G+ +A +VDP Sbjct: 89 QAARAFLGPQARLFAVRPAKAPGWNTRVMFSQPGLGDSESRAIFVDPVSLQVRGDLIVYG 148 Query: 109 TGALLPPVTGMEFFEFTERFHRWLLLPTEIGKIVLGTLALCLLMLALSGLYLRWPRRALS 168 T +LP T +++ HR L+L E+G+ A L + AL G++L W R Sbjct: 149 TSGVLPLRTTLDYL------HRNLML-GELGRNYSELAASWLWLGALGGVWLWWRART-- 199 Query: 169 LRAWLRLDFQLQGRSLLWNLHSVIGTWSLVMYVIFATTGAYWA-------------LDWF 215 R ++ L R LH+++G W ++ V F+ TG W+ L W Sbjct: 200 -RGRVQAARPLTAR----RLHALVGLWIVIGLVFFSATGLTWSRWAGERVNLMRAQLGWI 254 Query: 216 KDGVNTLAGETRRAPMARGEGKKKD----------GPNNQTPVALDLTQAWNTFLAKVPD 265 V+T + + A G+ D P P D+ Q+ +A+ Sbjct: 255 TPSVST---QLQPGAPAMSMGEHADHMAAHGMAMAMPAVDDPARFDMVQS----IARAGG 307 Query: 266 YQSAQLRL-PEQGTQ------------PLQIFFLGHDAPHERARSRMVVLPQSGEIKQLE 312 +A++ + P + Q P Q+ + DA R SR I +L Sbjct: 308 IDAAEIEIRPPRAAQQAWVVREVDRSWPTQVDTVAIDADRMRITSR-ADFASFPLIAKLI 366 Query: 313 RYEDKSTGGRFIAAIYPLHMGTYFGLPGRIIMAIASLMMPLFGITGWMLYLDRRRKKAA 371 R+ + HMG FG+ +I+MA + + G++++ RR A Sbjct: 367 RWGVDA------------HMGVLFGVANQILMAAFGIALTGLIALGYLMWWRRRPAAGA 413 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,705,571 Number of extensions: 116643 Number of successful extensions: 420 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 415 Number of HSP's successfully gapped: 4 Length of query: 850 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 757 Effective length of database: 1,179,515 Effective search space: 892892855 Effective search space used: 892892855 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)