BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0435.p
         (277 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310447|ref|YP_003774539.1| esterase [Herbaspirillum serope...   561   e-161
gi|300312950|ref|YP_003777042.1| lipase [Herbaspirillum seropedi...   156   1e-39
gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase fa...    71   9e-14

>gi|300310447|ref|YP_003774539.1| esterase [Herbaspirillum
           seropedicae SmR1]
          Length = 277

 Score =  561 bits (1446), Expect = e-161,   Method: Compositional matrix adjust.
 Identities = 276/277 (99%), Positives = 276/277 (99%)

Query: 1   MKIYRDYTSEELDAQYNVRARVPDFQDYFDRFARQGEAARQAHPHLADLPYGEEMLQSID 60
           MKIYRDYTSEELDAQYNVRARVPDFQDYFDRFARQGEAARQAHPHLADLPYGEE LQSID
Sbjct: 1   MKIYRDYTSEELDAQYNVRARVPDFQDYFDRFARQGEAARQAHPHLADLPYGEETLQSID 60

Query: 61  FFPAHSNGRPLLVFIHGGYWRSLDKHQFSHLALPYLEQDINVALINYRLAPQVRMGDIAA 120
           FFPAHSNGRPLLVFIHGGYWRSLDKHQFSHLALPYLEQDINVALINYRLAPQVRMGDIAA
Sbjct: 61  FFPAHSNGRPLLVFIHGGYWRSLDKHQFSHLALPYLEQDINVALINYRLAPQVRMGDIAA 120

Query: 121 DCGRALRLLHAKAPALRVDANAIWLMGHSAGAHLACVIAALRAAPVRGVCALSGLYDLEP 180
           DCGRALRLLHAKAPALRVDANAIWLMGHSAGAHLACVIAALRAAPVRGVCALSGLYDLEP
Sbjct: 121 DCGRALRLLHAKAPALRVDANAIWLMGHSAGAHLACVIAALRAAPVRGVCALSGLYDLEP 180

Query: 181 IRLSYLNEALHLSPADVQQASPLLLAMPDGVRAVLCAGGQESEEFLRQRDVYAANLRQSG 240
           IRLSYLNEALHLSPADVQQASPLLLAMPDGVRAVLCAGGQESEEFLRQRDVYAANLRQSG
Sbjct: 181 IRLSYLNEALHLSPADVQQASPLLLAMPDGVRAVLCAGGQESEEFLRQRDVYAANLRQSG 240

Query: 241 HQVEVVEAAQAHHLSIIDVAADGQSAFTRTVLRMMQD 277
           HQVEVVEAAQAHHLSIIDVAADGQSAFTRTVLRMMQD
Sbjct: 241 HQVEVVEAAQAHHLSIIDVAADGQSAFTRTVLRMMQD 277


>gi|300312950|ref|YP_003777042.1| lipase [Herbaspirillum seropedicae
           SmR1]
          Length = 289

 Score =  156 bits (395), Expect = 1e-39,   Method: Compositional matrix adjust.
 Identities = 99/268 (36%), Positives = 138/268 (51%), Gaps = 6/268 (2%)

Query: 11  ELDAQYNVRARVPDFQDYFDRFARQGEAARQAHPHLADLPYGEEMLQSIDFFPAHSNGRP 70
           E +A YN++A  PDF     ++ +   A        +D+ YG+  LQ  DF+ A    RP
Sbjct: 21  ECEAGYNLKAAFPDFPQVLQQWQQATAAIADTLLTESDISYGDAPLQRFDFYRAEGAQRP 80

Query: 71  LLVFIHGGYWRSLDKHQFSHLALPYLEQDINVALINYRLAPQVRMGDIAADCGRALRLLH 130
           LLVFIHGGYW+  DK     +A PY++  I+VA+INY LAPQ R+ D+  +    L  + 
Sbjct: 81  LLVFIHGGYWQGGDKRDIGFIAAPYVKAGISVAVINYSLAPQARIEDMVKEVQACLSTIA 140

Query: 131 AKAPALRVDANAIWLMGHSAGAHLACVIAALRA-APVRGVCALSGLYDLEPIRLSYLNEA 189
            +A  L +D + I LMGHSAG HLA  +AA     PV+ V A+SG++DL P+  + LN+A
Sbjct: 141 QQAERLGIDVDRISLMGHSAGGHLAAFVAAQPGRMPVQAVFAISGVFDLAPLIPTSLNKA 200

Query: 190 LHLSPADVQQASPLLLAMPDGVRAVLCAGGQESEEFLRQRDVYAANLRQ-SGHQVEVVEA 248
           L L        SP+L   P   R     G  E+ +F  Q  V A    Q   H V     
Sbjct: 201 LTLDQVRADALSPVLQTGPAQTRVHTIIGELETLQFHLQAAVMANCWPQVVAHHV----V 256

Query: 249 AQAHHLSIIDVAADGQSAFTRTVLRMMQ 276
              HH +++   AD  S+  R V+  M 
Sbjct: 257 PDTHHYTVLFPLADADSSVCRAVIAEMN 284


>gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase
           family protein [Herbaspirillum seropedicae SmR1]
          Length = 302

 Score = 71.2 bits (173), Expect = 9e-14,   Method: Compositional matrix adjust.
 Identities = 63/206 (30%), Positives = 92/206 (44%), Gaps = 17/206 (8%)

Query: 54  EMLQSIDFFPAHSNGRPLLVFIHGGYWRSLDKHQFSHLALPYLEQDINVALINYRLAPQV 113
           ++ +  D   A   GRP++VF +GG W    +  ++ +      + +   L +YRL P+V
Sbjct: 58  DIYRPADTASAPPQGRPVVVFFYGGSWNQGSRKDYAFVGEALASRGVIAVLPDYRLYPEV 117

Query: 114 RMGDIAADCGRALRLLHAKAPALRVDANAIWLMGHSAGAHLACVIA-------ALRAAP- 165
              D   D  +A+        AL  D   +++MGHSAGA+ A ++A       A  A P 
Sbjct: 118 SYPDFLQDSAQAVAWTLRSLQALGGDPQRVFVMGHSAGAYNAAMMALDARWLDAAGATPA 177

Query: 166 -VRGVCALSGLYDLEPIRLSYLNEALHL--SPADVQQASPLLLAMPDGVRAVLCAGGQES 222
            +RG   L+G YD  PI    +    H   SP D Q    +  + P    A+L A  Q+ 
Sbjct: 178 QLRGWIGLAGPYDFLPIENPDVKPVFHFPDSPPDSQPVRHVQASAPP---ALLIASHQDK 234

Query: 223 -EEFLRQRDVYAANLRQSGHQVEVVE 247
                R     AA LRQ  H V VVE
Sbjct: 235 LVSATRNTGGLAALLRQ--HGVPVVE 258


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.137    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 919,604
Number of extensions: 40519
Number of successful extensions: 172
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 171
Number of HSP's successfully gapped: 3
Length of query: 277
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 193
Effective length of database: 1,222,130
Effective search space: 235871090
Effective search space used: 235871090
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 130 (54.7 bits)