BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0433.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 876 0.0 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 239 4e-64 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 199 4e-52 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 104 2e-23 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 100 3e-22 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 876 bits (2264), Expect = 0.0, Method: Compositional matrix adjust. Identities = 447/447 (100%), Positives = 447/447 (100%) Query: 1 MSIHTSGALPTVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWG 60 MSIHTSGALPTVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWG Sbjct: 1 MSIHTSGALPTVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWG 60 Query: 61 VSKASLGPVFGAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQL 120 VSKASLGPVFGAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQL Sbjct: 61 VSKASLGPVFGAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQL 120 Query: 121 LVLRFITGFGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAY 180 LVLRFITGFGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAY Sbjct: 121 LVLRFITGFGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAY 180 Query: 181 GWHAVFWVGGAVPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEV 240 GWHAVFWVGGAVPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEV Sbjct: 181 GWHAVFWVGGAVPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEV 240 Query: 241 VVKETKAEGMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAV 300 VVKETKAEGMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAV Sbjct: 241 VVKETKAEGMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAV 300 Query: 301 LIGTSLQVGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIV 360 LIGTSLQVGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIV Sbjct: 301 LIGTSLQVGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIV 360 Query: 361 AGFCIVGGQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASL 420 AGFCIVGGQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASL Sbjct: 361 AGFCIVGGQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASL 420 Query: 421 FLAAAVPALISALTIARLHRTAQGVSA 447 FLAAAVPALISALTIARLHRTAQGVSA Sbjct: 421 FLAAAVPALISALTIARLHRTAQGVSA 447 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 239 bits (609), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 151/429 (35%), Positives = 251/429 (58%), Gaps = 7/429 (1%) Query: 9 LPTVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGP 68 + T+ V Q+++ RF F + ++ LC L L+ DG+D+ G V P ++ +W +S G Sbjct: 1 MKTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA 60 Query: 69 VFGAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITG 128 + LFGM+ G+ + P+ DR GR+ + +S + F++ + T+ + V RF+ G Sbjct: 61 LGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAG 120 Query: 129 FGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWV 188 G G +MPNA+AL+ E++P R T + ++ G+++G + ++P +GW ++F+ Sbjct: 121 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFF- 179 Query: 189 GGAVPLVLGLAMLVW-LPESIQFLVLHRRPRTQVAR-WLRKLDPNIVIDDKTEVVVKETK 246 AVPLVL L +++W LPES+ FL+ R+ R + AR L ++DP I T++V + K Sbjct: 180 AAAVPLVL-LPVILWKLPESVGFLL--RQGRLEQARAMLTQVDPQARIATDTQLVQADVK 236 Query: 247 AEGMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSL 306 G P+ LF+ RA T+++W+ F L+ +Y L +WLP L+ AGYS ++ +L Sbjct: 237 GAGAPMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLAL 296 Query: 307 QVGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAGFCIV 366 GGV+G + G RF +V+ S F + ++IAL+G + P +L++ +IVAG + Sbjct: 297 NFGGVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTP-VLYLLIIVAGATTI 355 Query: 367 GGQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFLAAAV 426 G Q + A FYP +RSTG+GWA G+GR G++VGP++GG L+A FL A+ Sbjct: 356 GTQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAI 415 Query: 427 PALISALTI 435 P L++AL + Sbjct: 416 PGLVAALAM 424 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 199 bits (505), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 142/439 (32%), Positives = 224/439 (51%), Gaps = 17/439 (3%) Query: 11 TVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGPVF 70 TV + ++++ G F FQ ++ L L +++DGFD Q +G+ P++I +WG+++ + P Sbjct: 11 TVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAPAV 70 Query: 71 GAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFF--ALCMLA-TPMVTTLDQLLVLRFIT 127 AGL GM +GS DR+GRR +I S F A ++A +P VTT+ +LRF Sbjct: 71 AAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIA---ILRFCA 127 Query: 128 GFGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFW 187 G G+G +P++ + EFSP+ R + +G G + A++P YGW +F Sbjct: 128 GLGIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFL 187 Query: 188 VGGAVPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVID----DKTEVVVK 243 +GG +P+ + + +PES ++L H ++ R L+++ + D D E + Sbjct: 188 IGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFADAREQAI- 246 Query: 244 ETKAEGMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIG 303 ET+A ALF G+A T+ LW+ FMNL+ +Y +WLPT++ G S S+A Sbjct: 247 ETRAG---FGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGL 303 Query: 304 TSLQVGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKL--ATVPALLFVAVIVA 361 T+ GGV G L FI RFG + C L A S + L A+ LL V + + Sbjct: 304 TAYNFGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGIGLH 363 Query: 362 GFCIVGGQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASL- 420 G + Q + AL YPT +R+TG AL GR+G+++ G +I L Sbjct: 364 GLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVITQGGAAGYLA 423 Query: 421 FLAAAVPALISALTIARLH 439 LA A+ + AL + R H Sbjct: 424 MLAGAMLMVFVALLVVRKH 442 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 98/394 (24%), Positives = 175/394 (44%), Gaps = 25/394 (6%) Query: 18 VEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGPVFGAGLFGM 77 +E F + ++ G + D F ++ +V P ++ W +S A +G + +G G Sbjct: 33 IENVPFSRWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQ 92 Query: 78 LLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITGFGLGSIMPN 137 ++G+L + +RYGR P ++ ++ + + + L + RF+ G G+G +P Sbjct: 93 VIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPV 152 Query: 138 AMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWVGGAVPLVLG 197 A + E S + R +L F +G A V A L+P +GW +F VGG +P +L Sbjct: 153 AATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPRFGWEYMFLVGG-IPGILV 211 Query: 198 LAMLVWLPESIQFLVLHRR--PRTQVARWLRKLDPNIVIDDKTEVVVKETKAEGMPVAA- 254 ++ LPES ++L+ R ++ R L P +D +T+ E + + A Sbjct: 212 ALLITRLPESPRWLIARGRLDDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAG 271 Query: 255 ---------LFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTS 305 LF G T+++WL+ + ++NWLP+L Y T + + S Sbjct: 272 KRQRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSL-----YKTVYHLPLQES 326 Query: 306 LQVGGVIGTLS------LGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVI 359 L++ V LS I R G R + F+L+ +AL+G L A + + Sbjct: 327 LRMASVSNVLSACAVLGCALLIDRVGRRRWAIASFILSGALLALLGVLKAESAWSVMFLA 386 Query: 360 VAGFCIVGGQPAVNAL-AGTFYPTTLRSTGIGWA 392 + + ++G + L YPT +R+ G G A Sbjct: 387 SSAYAVMGTTTVLLYLYTPEIYPTRMRAIGTGLA 420 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 100 bits (249), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 105/415 (25%), Positives = 193/415 (46%), Gaps = 8/415 (1%) Query: 13 HVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGPVFGA 72 V+Q ++ G FQ+ L ++ GL + D V ++G+ AP I +G++ Sbjct: 7 SVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTM 66 Query: 73 GLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITGFGLG 132 GMLLG+ V + DR GRRPVL+ + + A +A+ LL LR +TG G+G Sbjct: 67 FFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVG 126 Query: 133 SIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYG---WHAVFWVG 189 +P ++ EF PS+ R ++++ + +G V A + ++G W +F+V Sbjct: 127 GTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLA-VLALVAVSWGNDAWRVIFFVT 185 Query: 190 GAVPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEVVVKETKAEG 249 G +P ++G+ + ++PES + L+R ++ AR + + + ++ E Sbjct: 186 G-LPALVGVVLRFYVPESPMY--LNRSGKSDAARQVLERVAKVNGSSTPIPPLQPEVVER 242 Query: 250 MPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSLQVG 309 P++ALF ++ L+L + I Y + +LP + G+ + L + Sbjct: 243 KPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLALV 302 Query: 310 GVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAGFCIVGGQ 369 + G ++R+G L +L+ + L L P L+ + ++ F ++G Sbjct: 303 QLPGYALSAYGVERWGRKPTLVGFLVLSAVGCMLY-SLGKDPMLVVGSTLLLSFSLLGTW 361 Query: 370 PAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFLAA 424 A+ A YPT LR++G+G A + R G + P I ++A +T A L+A Sbjct: 362 AALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASHFTLALAMLSA 416 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.143 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,421,519 Number of extensions: 61861 Number of successful extensions: 535 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 493 Number of HSP's successfully gapped: 29 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)