BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0428.p (676 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310454|ref|YP_003774546.1| flagellar hook-associated prote... 1303 0.0 gi|300311383|ref|YP_003775475.1| flagellar hook-associated 2 pro... 917 0.0 gi|300312832|ref|YP_003776924.1| flagellar capping protein [Herb... 736 0.0 >gi|300310454|ref|YP_003774546.1| flagellar hook-associated protein 2 [Herbaspirillum seropedicae SmR1] Length = 676 Score = 1303 bits (3371), Expect = 0.0, Method: Compositional matrix adjust. Identities = 671/676 (99%), Positives = 674/676 (99%) Query: 1 MATSTGTISTSAGPLDVATLVNQLISVESKSRLTPLKNKESSFNTLISAYGSLKNAFTSY 60 MATSTGTISTSAGPLDVATLVNQLISVESKSRLTPLKNKESSFNTLISAYGSLKNAFTSY Sbjct: 1 MATSTGTISTSAGPLDVATLVNQLISVESKSRLTPLKNKESSFNTLISAYGSLKNAFTSY 60 Query: 61 QSALKTLTAASFSAQKTTLSNAGTGTNLTTDPFTADTNTDESTKVLAQKLQSAGFTAGQT 120 QSALKTLTAASFSAQKTTLSNAGTGTNLTTDPFTADTNTDESTKVLAQKLQSAGFTAGQT Sbjct: 61 QSALKTLTAASFSAQKTTLSNAGTGTNLTTDPFTADTNTDESTKVLAQKLQSAGFTAGQT 120 Query: 121 FNAGDSVAIKVGTNQPTFITLQANATLAGVRDAINAAKAGVTASITTDGSGDHLVLESNT 180 FNAGDSVAIKVGTNQPTFITLQANATLAGVRDAINAAKAGVTASITTDGSGDHLVLESNT Sbjct: 121 FNAGDSVAIKVGTNQPTFITLQANATLAGVRDAINAAKAGVTASITTDGSGDHLVLESNT 180 Query: 181 GDTANTIRITANNSLSSLAYDPSSNVSSTVTQIQAPRDASKAAAGKYNIAVNQLAQAVKV 240 G TANTI+ITANNSLSSLAYDPSSNVSSTVTQIQAPRDASKAAAGKYNIAVNQLAQAVKV Sbjct: 181 GGTANTIKITANNSLSSLAYDPSSNVSSTVTQIQAPRDASKAAAGKYNIAVNQLAQAVKV 240 Query: 241 SSAGIAPGSTFDNGVLAIKTGNGSTAIIQPKTNSLAGVRDAINASDAGVNASIVNDGTND 300 SSAGIAPGSTFDNGVLAIKTGNGSTAIIQPKTNSLAGVRDAINASDAGVNASIVNDGTND Sbjct: 241 SSAGIAPGSTFDNGVLAIKTGNGSTAIIQPKTNSLAGVRDAINASDAGVNASIVNDGTND 300 Query: 301 HLVLTAKDAGSANTLRVTGTGNFAAFNFDPSGTITTTGVATNQTYSSGSLALQVGSKSFT 360 HLVLTAKDAGSANTLRVTGTGNFAAFNFDPSGTITTTGVATNQTYSSGSLALQVGSKSFT Sbjct: 301 HLVLTAKDAGSANTLRVTGTGNFAAFNFDPSGTITTTGVATNQTYSSGSLALQVGSKSFT 360 Query: 361 INPTDLDGSGAIGLNDVMKAINDANTGVTASITNDGSKDHLVLTPTGTDAVKLTGAGDYS 420 INPTDLDGSGAIGLNDVMKAINDANTGVTASITNDGSKDHLVLTP+GTDAVKLTGAGDYS Sbjct: 361 INPTDLDGSGAIGLNDVMKAINDANTGVTASITNDGSKDHLVLTPSGTDAVKLTGAGDYS 420 Query: 421 DLAGSTMGQLAKAQDAKLTVDGVTVTITSNKVTNVISGVTLNLSKVTTSADNFSLSINND 480 DLAGS+MGQLAKAQDAKLTVDGVTVT TSNKVTNVISGVTLNLSKVTTSADNFSLSINND Sbjct: 421 DLAGSSMGQLAKAQDAKLTVDGVTVTSTSNKVTNVISGVTLNLSKVTTSADNFSLSINND 480 Query: 481 TSGLSTAANTFVTAYNTLAKAVANLTKQTPSTTKGQASTGSPLASESSVLALMGQVRNTM 540 TSGLSTAANTFVTAYNTLAKAVANLTKQTPSTTKGQASTGSPLASESSVLALMGQVRNTM Sbjct: 481 TSGLSTAANTFVTAYNTLAKAVANLTKQTPSTTKGQASTGSPLASESSVLALMGQVRNTM 540 Query: 541 LGALGDNGATNLSQIGISFQKDGTLALDATKLTNAANKDFDGITQLFTSATNGVVPKLQK 600 LGALGDNGATNLSQIGISFQKDGTLALDATKLTNAANKDFDGITQLFTSATNGVVPKLQK Sbjct: 541 LGALGDNGATNLSQIGISFQKDGTLALDATKLTNAANKDFDGITQLFTSATNGVVPKLQK 600 Query: 601 LMDGILGESGVLASKTKGLQDSLKIVTDQQTAANARLQDLKDTYTNQFNRLNVTLATMQS 660 LMDGILGESGVLASKTKGLQDSLKIVTDQQTAANARLQDLKDTYTNQFNRLNVTLATMQS Sbjct: 601 LMDGILGESGVLASKTKGLQDSLKIVTDQQTAANARLQDLKDTYTNQFNRLNVTLATMQS 660 Query: 661 RQSYLTQQLAKLSKSS 676 RQSYLTQQLAKLSKSS Sbjct: 661 RQSYLTQQLAKLSKSS 676 >gi|300311383|ref|YP_003775475.1| flagellar hook-associated 2 protein [Herbaspirillum seropedicae SmR1] Length = 690 Score = 917 bits (2371), Expect = 0.0, Method: Compositional matrix adjust. Identities = 482/690 (69%), Positives = 575/690 (83%), Gaps = 15/690 (2%) Query: 1 MATSTGTISTSAGPLDVATLVNQLISVESKSRLTPLKNKESSFNTLISAYGSLKNAFTSY 60 MATSTGTISTSAGPLDVATLV+QLIS ES+S+LTPL + SS+NTLISAYGSLK+A ++Y Sbjct: 1 MATSTGTISTSAGPLDVATLVSQLISAESQSQLTPLTTQASSYNTLISAYGSLKSAMSTY 60 Query: 61 QSALKTLTAASFSAQKTTLSNAGTGTNLTTDPFTADTNTDESTKVLAQKLQSAGFTAGQT 120 QSA+K +T+ASFSAQK+++SNAGTG+NLTTDPF+AD N D+STK+LAQK+QSAG ++G T Sbjct: 61 QSAIKAMTSASFSAQKSSVSNAGTGSNLTTDPFSADINGDDSTKILAQKIQSAGISSGTT 120 Query: 121 FNAGDSVAIKVGTNQPTFITLQANATLAGVRDAINAAKAGVTASITTDGSGDHLVLESNT 180 FNAGDS+AIK+GTN P F+TLQAN+TLAGVRDAINA K G TASI TDG+GDHLVLESNT Sbjct: 121 FNAGDSIAIKIGTNSPVFVTLQANSTLAGVRDAINAGKTGATASIVTDGAGDHLVLESNT 180 Query: 181 GDTANTIRITANNSLSSLAYDPSSNVSSTVTQIQAPRDASKAAAGKYNIAVNQLAQAVKV 240 G TANTI++TANNSLS YDPSS+ ST+TQ QAPRDA+KAA+G Y +AV+QLAQA K+ Sbjct: 181 GGTANTIKVTANNSLSGFFYDPSSSTPSTMTQTQAPRDATKAASGNYAVAVSQLAQAEKL 240 Query: 241 SSAGIAPGSTFDNGVLAIKTGNGSTAIIQPKTNSLAGVRDAINASDAGVNASIVNDGTND 300 SSA IAPGSTFDNG+LAIKTG+GST II+P TN+LAGVRDAIN+SDAGVNA+IV+DGTND Sbjct: 241 SSASIAPGSTFDNGLLAIKTGSGSTTIIKPTTNTLAGVRDAINSSDAGVNATIVSDGTND 300 Query: 301 HLVLTAKDAGSANTLRVTGTGNFAAFNFDPSGTITTTGVATNQTYSSGSLALQVGSKSFT 360 HLV+TAK++GS NT+++TGT NFA FN DPSGT+T+ VA+ QTYS+G+L L+VG SF Sbjct: 301 HLVVTAKNSGSTNTIKITGTENFAVFNSDPSGTVTSPNVASGQTYSAGNLTLKVGDSSFN 360 Query: 361 INPTDLDGSGAIGLNDVMKAINDANTGVTASITNDGSKDHLVLTPTG---TDAVKLTGAG 417 I P +GSG I L V AIN+AN GVTASITNDGS DHLVLTPTG T AV LTG Sbjct: 361 IAPV-ANGSGNIDLTQVKLAINNANAGVTASITNDGSNDHLVLTPTGSSATAAVSLTGTS 419 Query: 418 DYSDLAGSTMGQLAKAQDAKLTVDGVTVTITSNKVTNVISGVTLNLSKVTTSADNFSLSI 477 DY+ L+ S MGQL KAQDAKL++DGV VT +NKV NVISGVTLNLSKVTTSADNF+L+I Sbjct: 420 DYAGLSMSGMGQLLKAQDAKLSIDGVNVTSPTNKVENVISGVTLNLSKVTTSADNFTLNI 479 Query: 478 NNDTSGLSTAANTFVTAYNTLAKAVANLTKQTPSTTKGQASTGSPLASESSVLALMGQVR 537 +NDTSG++TAAN+FVTAYNTLAKAVA +T QTPSTT GQA+T +PLASESSV +M Q+R Sbjct: 480 SNDTSGITTAANSFVTAYNTLAKAVAGMTAQTPSTTLGQANTSAPLASESSVQTIMSQLR 539 Query: 538 NTMLGAL-GDNGATNLSQIGISFQKDGTLALDATKLTNAANKDFDGITQLF--------- 587 NT+ + G NG LS IGISFQKDGTLALD+TKL+ AA +F GI LF Sbjct: 540 NTLFSTVDGGNGINTLSDIGISFQKDGTLALDSTKLSTAATNNFAGIANLFAGTDPDPTN 599 Query: 588 -TSATNGVVPKLQKLMDGILGESGVLASKTKGLQDSLKIVTDQQTAANARLQDLKDTYTN 646 TS+ NG+V KLQ LM +L ++G++A+KT GLQ SLKI D+QTA +RL +LKD YTN Sbjct: 600 TTSSKNGIVTKLQTLMTSLLSDNGIIATKTSGLQASLKINQDRQTAVQSRLSNLKDAYTN 659 Query: 647 QFNRLNVTLATMQSRQSYLTQQLAKLSKSS 676 QFN+LN+TL++MQS QSYLTQQLA L+KSS Sbjct: 660 QFNQLNLTLSSMQSTQSYLTQQLANLAKSS 689 >gi|300312832|ref|YP_003776924.1| flagellar capping protein [Herbaspirillum seropedicae SmR1] Length = 680 Score = 736 bits (1899), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/680 (56%), Positives = 506/680 (74%), Gaps = 7/680 (1%) Query: 1 MATSTGTISTSAGPLDVATLVNQLISVESKSRLTPLKNKESSFNTLISAYGSLKNAFTSY 60 MATSTG+ISTSAGPLDVA LVNQL+SVESKSRLTPL +K SFN LISAYG L A T+Y Sbjct: 1 MATSTGSISTSAGPLDVANLVNQLVSVESKSRLTPLASKADSFNALISAYGKLGTALTTY 60 Query: 61 QSALKTLTAASFSAQKTTLSNAGTGTNLTT--DPFTADTNTDESTKVLAQKLQSAGFTAG 118 Q+AL LTAA FSAQK +SN G ++ + D +AD N+D STK AQ ++SAGF +G Sbjct: 61 QNALAELTAAKFSAQKAAVSNTGRSSDGKSGNDAVSADINSDASTKAQAQIIKSAGFASG 120 Query: 119 QTFNAGDSVAIKVGTNQPTFITLQANATLAGVRDAINAAKAGVTASITTDGSGDHLVLES 178 F AGDSVAIKVG P FITL+A+AT+ G+RD INAA+AGVTAS+ D +GDHLVLES Sbjct: 121 HLFKAGDSVAIKVGEASPHFITLKADATINGLRDTINAARAGVTASVVRDDAGDHLVLES 180 Query: 179 NTGDTANTIRITANNSLSSLAYDPSSNVSSTVTQIQAPRDASKAAAGKYNIAVNQLAQAV 238 NT TAN +++ ANNSLS++++D + +TQ QA RDA+ AA G Y I+V QLAQ+ Sbjct: 181 NTAGTANLLKVRANNSLSAISFDAQQSRPGAMTQTQAARDATSAAPGSYAISVEQLAQSH 240 Query: 239 KVSSAGIAPGSTFDNGVLAIKTGNGSTAIIQPKTNSLAGVRDAINASDAGVNASIVNDGT 298 K+SSAGIAPGSTFD GVLAIK G+GST +I+P +N+LAGVRDAINA+ AGVNASIV+DG Sbjct: 241 KLSSAGIAPGSTFDLGVLAIKAGSGSTTLIKPASNTLAGVRDAINAAAAGVNASIVSDGK 300 Query: 299 NDHLVLTAKDAGSANTLRVTGTGNFAAFNFDPSGTITTTGVATNQTYSSGSLALQVGSKS 358 +HLVLTAK++G+ N LR++GTG+FA F+ DPSG +T V + +S G++ L VG+++ Sbjct: 301 QEHLVLTAKESGANNALRISGTGDFAVFSSDPSGNASTASVTPEKRFSQGAVNLSVGART 360 Query: 359 FTINPTDLDGSGAIGLNDVMKAINDANTGVTASITNDGSKD----HLVLTPTGTDAVKLT 414 ++ P A GL DV +AIN+A GV+A++ +G D LVL+ +G+D V L Sbjct: 361 ISLKPASATAGAAPGLADVARAINEAKAGVSATVQREGQGDKAVERLVLSASGSDPVSLR 420 Query: 415 GAGDYSDLAGSTMGQLAKAQDAKLTVDGVTVTITSNKVTNVISGVTLNLSKVTTSADNFS 474 G+GDY+ LA S MGQL K QDA+L++DGV +T TSN + + ISGVTLNL+K T++ D + Sbjct: 421 GSGDYAVLAASAMGQLQKPQDARLSIDGVAITATSNTIKDAISGVTLNLNKTTSATDKLT 480 Query: 475 LSINNDTSGLSTAANTFVTAYNTLAKAVANLTKQTPSTTKGQASTGSPLASESSVLALMG 534 L+I+ND+SG+ A N+FV A+NTLAKA+A +T+Q P+ +GQA LA ES V AL G Sbjct: 481 LAISNDSSGVGAAVNSFVNAFNTLAKAMAEMTRQAPAKVRGQAGEQGLLAGESMVRALTG 540 Query: 535 QVRNTMLGALGDNGATNLSQIGISFQKDGTLALDATKLTNAANKDFDGITQLFTSATNGV 594 ++RN +L ++ N +L+ IG+ F+KDGTL LD+ +L A ++F+ + QLF + G+ Sbjct: 541 ELRNAVLASMPGNALGSLTAIGVGFKKDGTLDLDSARLAKAGERNFEAVAQLF-AGKEGL 599 Query: 595 VPKLQKLMDGILGESGVLASKTKGLQDSLKIVTDQQTAANARLQDLKDTYTNQFNRLNVT 654 V + + L+D +LGE G+LA KT+GLQ SLKIV+DQQ AA ARL +L+D+YTNQFNRLNVT Sbjct: 600 VARTRSLLDKVLGEQGLLAGKTRGLQGSLKIVSDQQAAARARLANLRDSYTNQFNRLNVT 659 Query: 655 LATMQSRQSYLTQQLAKLSK 674 L+ MQ+ Q+YLTQQLA+L + Sbjct: 660 LSKMQASQNYLTQQLARLRR 679 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.307 0.121 0.317 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,778,818 Number of extensions: 73464 Number of successful extensions: 327 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 288 Number of HSP's successfully gapped: 6 Length of query: 676 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 584 Effective length of database: 1,184,250 Effective search space: 691602000 Effective search space used: 691602000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 134 (56.2 bits)