BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0426.p (458 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 934 0.0 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 227 2e-60 gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 208 9e-55 gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 188 7e-49 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 141 1e-34 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 934 bits (2413), Expect = 0.0, Method: Compositional matrix adjust. Identities = 456/458 (99%), Positives = 457/458 (99%) Query: 1 MQTSALAALDRRHLIHPVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNT 60 MQTSALAALDRRHLIHPVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNT Sbjct: 1 MQTSALAALDRRHLIHPVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNT 60 Query: 61 GYGQESVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAV 120 GYGQESVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAV Sbjct: 61 GYGQESVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAV 120 Query: 121 DAAIRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLP 180 DAAIRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLP Sbjct: 121 DAAIRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLP 180 Query: 181 SPYPYRSELQGDEAIIAASVQSLRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAM 240 SPYPYRSELQGDEAIIAASVQ+LRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAM Sbjct: 181 SPYPYRSELQGDEAIIAASVQALRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAM 240 Query: 241 QLACNELDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDR 300 QLACNELDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDR Sbjct: 241 QLACNELDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDR 300 Query: 301 VYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRA 360 VYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRA Sbjct: 301 VYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRA 360 Query: 361 LLVHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDNIL 420 LL HPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDNIL Sbjct: 361 LLAHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDNIL 420 Query: 421 GFAPALCYTQEDFALMFARLKAILDEVLAQPEVRQAVR 458 GFAPALCYTQEDFALMFARLKAILDEVLAQPEVRQAVR Sbjct: 421 GFAPALCYTQEDFALMFARLKAILDEVLAQPEVRQAVR 458 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 227 bits (578), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 144/419 (34%), Positives = 223/419 (53%), Gaps = 17/419 (4%) Query: 34 SGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQRLPYATGYFHFASEPAI 93 +G+G L D K+ +DA G G+G VI A +Q+ L YA F F + PA Sbjct: 18 AGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSF-FTTAPAE 76 Query: 94 LLAKKLVDISPASLQHVYFTLGGSDAVDAAIRYITHYHHSIGKPGKQHFISLQRGYHGSS 153 LA L D +P SL HVYF GGS+AV+AA++ Y+ +G+P ++H I+ ++ YHG++ Sbjct: 77 ELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQPQRRHIIARRQSYHGNT 136 Query: 154 SVGAGLTALPNFHYHFNLPLPTQ-HYLPSPYPYRSELQGDEAI--IAASVQSLRDKVAEL 210 +GA + +P+ + H++ Y YR+ G+ + + L K+ L Sbjct: 137 -LGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDVQYVQRLADELEQKILSL 195 Query: 211 GADNVAAFFCEPIQGSGGVVVPPKG-WLKAMQLACNELDILFVVDEVITGFGRTGPMFAC 269 GAD V AF E + G+ VPP G + + ++ C++ +L ++DEV++G GRTG +FAC Sbjct: 196 GADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVMSGMGRTGYLFAC 255 Query: 270 LDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMSTVVGHGQTYSAHPVSAA 329 ++ V PD++ +AKGL AGY P+GA++ SD +Y+ + G S HG TY H + A Sbjct: 256 EEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRG---SGFFQHGHTYIGHATACA 312 Query: 330 VALEVLRLYEEGGLLDNGRRLEPVFAGGLR-ALLVHPLVGDARSRGLLGALELVADKQTK 388 A+ V + +E LL+N R+ LR A VGD R RGL +ELVA++ +K Sbjct: 313 AAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLFVGVELVAERSSK 372 Query: 389 ARFAPELKLHDRIFQAAYRHGLVFRAFGDNILG-------FAPALCYTQEDFALMFARL 440 +P+L+ H R+ A + GL+ G I G AP + D + + RL Sbjct: 373 LPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPFIASSNDISEIVQRL 431 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 208 bits (529), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 142/432 (32%), Positives = 214/432 (49%), Gaps = 15/432 (3%) Query: 17 PVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQ 76 P T+ R + P +L S +G + D +LD +GLWCV G+ ++ A + + Sbjct: 18 PFTANRNFKA-APRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVSAVQKSVA 76 Query: 77 RLPYATGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAVDAAIRYITHYHHSIGK 136 L YA F A LA++L + +V++T GS+AVD A++ YH + G Sbjct: 77 TLDYAPS-FQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAYHRAKGN 135 Query: 137 PGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPL-PTQHYLPSPYPYRSE--LQGDE 193 + I +RGYHG G + + F+ L P +LP + +G+ Sbjct: 136 ATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNAFTKGEP 195 Query: 194 AIIAASVQSLRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAMQLACNELDILFVV 253 A L V A N+AA EP+ GS GV++PPKG+LK ++ C + IL + Sbjct: 196 EYGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLKRLREICTKHGILLIF 255 Query: 254 DEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMST 313 DEVITGFGR G FA DV+PD+ T AKGLT G PMGAV + +++ +G P Sbjct: 256 DEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNG-PDGI 314 Query: 314 VVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRALLVHPLVGDARSR 373 + HG TYS HP++ A L L +++ G+L+N + + F AL P V D R+ Sbjct: 315 ELFHGYTYSGHPLACAAGLASLEVFQTEGVLENAQAVADYFQEAAHALRGLPHVIDVRTI 374 Query: 374 GLLGALELVA-DKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDNILGFAPALCYTQED 432 GL+ +EL + + AR +D F A+ G++ R GD I+ +P L ++ Sbjct: 375 GLIAGIELASIPGRVGAR------AYD-AFVRAFNDGILIRVTGD-IIALSPPLIINKQQ 426 Query: 433 FALMFARLKAIL 444 +F +L AIL Sbjct: 427 IDELFGKLAAIL 438 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 188 bits (478), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 122/427 (28%), Positives = 203/427 (47%), Gaps = 11/427 (2%) Query: 3 TSALAALDRRHLIHPVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGY 62 +SAL A R + HP T +R HE P+ + G +L D ++ LDA S W G+ Sbjct: 6 SSALVARSLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWVNLFGH 65 Query: 63 GQESVIRAATEQMQRLPYA--TGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAV 120 + A EQ+ +L +A G+ H EP + L+++L ++ +L H ++ G+ AV Sbjct: 66 TNTRINAALVEQLGQLEHAMLAGFTH---EPVVALSEQLAALTGQALGHCFYASDGASAV 122 Query: 121 DAAIRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLP 180 + A++ H + G+P K FI + GYHG + G+T P F + L H Sbjct: 123 EIALKMSVHAWRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAHVAA 182 Query: 181 SPYPYRSELQGDEAIIAASVQSLRDKVAELGADNVAAFFCEP-IQGSGGVVVPPKGWLKA 239 SP ++ +AA +++ + +AA EP +Q + G+ + G+++ Sbjct: 183 SPDARQAAPGESAEEVAARALDDVERILQQRQGRIAALIVEPLVQCAAGMAMHDPGYVRG 242 Query: 240 MQLACNELDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSD 299 ++ C+ + DE+ G GRTG FAC V PDL+ ++KG++ GY P+ VM S+ Sbjct: 243 LRSLCDRYGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMSSE 302 Query: 300 RVYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLR 359 +Y D + H +Y+ +P++ AL L ++ E +L+ R PV A L+ Sbjct: 303 AIYQAFYDDDVRRGFL-HSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAALQ 361 Query: 360 ALLVHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDNI 419 L P V R RG++ A + V + A A ++ +AA R LV R G ++ Sbjct: 362 PLAQDPRVRHVRQRGMIWAYDAVLEGSAAATTAFSRRM---AAEAAQREVLV-RPIGASL 417 Query: 420 LGFAPAL 426 P L Sbjct: 418 YLMPPYL 424 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 141 bits (356), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 121/425 (28%), Positives = 195/425 (45%), Gaps = 39/425 (9%) Query: 29 PLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQRLPYATGYFHFA 88 P+ + G D +D +G+ + G V+ A +Q + L ++ Sbjct: 48 PIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHS---MEVN 104 Query: 89 SEPAILLAKKLVDISPASLQH---VYFTLGGSDAVDAAIRYITHYHHSIGKPGKQHFISL 145 S LA K+ +I P L+ +FT GGSDA++AA+++ G+ I+ Sbjct: 105 SSKRTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRVT------GRHQIIAF 158 Query: 146 QRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSPYPYR----SELQGDEAIIAASVQ 201 GYHG + LT + F + + P PY YR + + E I V Sbjct: 159 HGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEYVD 218 Query: 202 SLRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAMQLACNELDILFVVDEVITGFG 261 L + AD+VAA EP+QG GG V P +L+ ++ AC+ L +VDEV G G Sbjct: 219 YLLN-TPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAG 277 Query: 262 RTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMSTVVGHGQTY 321 RTG M+A V+PD++T KG+ G PM ++M + I DG+ T+ Sbjct: 278 RTGKMWAVEHSGVKPDMLTFGKGI-GGDMPMAGLVMRSDLAAKIPDGS-------QPNTF 329 Query: 322 SAHPVSAAVALEVLRLYEEG--GLLDNGRRLEPVFAGGLRALLVHPLVGDARSRGLLGAL 379 +A+ +SAAVAL + + ++ L++ L +R+ P VG+ R RGL+ + Sbjct: 330 AANSISAAVALTNISILQDPRLDLVNRAHTLGLEAQERIRS-FNSPWVGEVRGRGLMIGI 388 Query: 380 ELVADKQTKARFAPEL--KLHDRIFQAAYRHGLVFRAFG--DNILGFAPALCYTQEDFAL 435 ELV +++T+ + E KL D + HG++ G N++ P+L + +L Sbjct: 389 ELVENRETREPLSREKLGKLMDYVVG----HGVLMIPCGRYTNVMRVMPSLTIPR---SL 441 Query: 436 MFARL 440 MF L Sbjct: 442 MFKGL 446 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,565,890 Number of extensions: 71843 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 9 Length of query: 458 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 370 Effective length of database: 1,203,190 Effective search space: 445180300 Effective search space used: 445180300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)