BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0424.p (382 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 770 0.0 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 447 e-127 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 325 4e-90 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 279 2e-76 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 243 1e-65 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 770 bits (1988), Expect = 0.0, Method: Compositional matrix adjust. Identities = 380/382 (99%), Positives = 380/382 (99%) Query: 1 VAYAASYVEWFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIA 60 VAYAASYVEWFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIA Sbjct: 112 VAYAASYVEWFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIA 171 Query: 61 PALAAGCTVVCKPAEDTPLTSLALVRLAQEAGVPAGVINIVTASRERTPEVVDVWLADGR 120 PALAAGCTVVCKPAEDTPLTSLALVRLAQEAGVP GVINIVTASRERTPEVVDVWLADGR Sbjct: 172 PALAAGCTVVCKPAEDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGR 231 Query: 121 VRKISFTGSTAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNG 180 VRKISFTGSTAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNG Sbjct: 232 VRKISFTGSTAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNG 291 Query: 181 GQTCVSPNRIYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVR 240 GQTCVSPNRIYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVR Sbjct: 292 GQTCVSPNRIYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVR 351 Query: 241 DAIAGGARVITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATED 300 DAIA GARVITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATED Sbjct: 352 DAIARGARVITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATED 411 Query: 301 EVIAAANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYG 360 EVIAAANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYG Sbjct: 412 EVIAAANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYG 471 Query: 361 REGSVHGLDDYLHTKYVCQGQL 382 REGSVHGLDDYLHTKYVCQGQL Sbjct: 472 REGSVHGLDDYLHTKYVCQGQL 493 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 447 bits (1149), Expect = e-127, Method: Compositional matrix adjust. Identities = 225/381 (59%), Positives = 283/381 (74%), Gaps = 9/381 (2%) Query: 1 VAYAASYVEWFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIA 60 + YAAS++EWF EE R GD IP+P R++ +KE +GV AAITPWNFPAAMI RK Sbjct: 110 IGYAASFIEWFAEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAG 169 Query: 61 PALAAGCTVVCKPAEDTPLTSLALVRLAQEAGVPAGVINIVTASRERTPEVVDVWL-ADG 119 PALAAGC +V KPAE TP ++LAL LA+ AG+PAGV ++VT TP+ + + ++ Sbjct: 170 PALAAGCPMVLKPAEATPFSALALAVLAERAGIPAGVFSVVTG----TPKGIGGEMTSNP 225 Query: 120 RVRKISFTGSTAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRN 179 VRKISFTGST VGK L + SA ++KKLSLELGGNAPFIVFDDAD+DAA++G +A+K+RN Sbjct: 226 IVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRN 285 Query: 180 GGQTCVSPNRIYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHV 239 GQTCV NRIYVQ+ VYDAF KL V KVG T+ GP+IN +A+ K++QHV Sbjct: 286 AGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHV 345 Query: 240 RDAIAGGARVITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATE 299 DA+A GARV+ GGKR G +++ PTVLADVT AMQ + EETFGP+AP+ RF T+ Sbjct: 346 ADAVAKGARVLLGGKR---HALG-HSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTD 401 Query: 300 DEVIAAANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGY 359 +EV+A AN T FGLA+YFYS D+ RI RV + LESG+VG+N G ++ E APFGGVK+SG Sbjct: 402 EEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGL 461 Query: 360 GREGSVHGLDDYLHTKYVCQG 380 GREGS +G+DDYL KY+C G Sbjct: 462 GREGSHYGIDDYLVVKYLCMG 482 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 325 bits (833), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 169/376 (44%), Positives = 233/376 (61%), Gaps = 7/376 (1%) Query: 4 AASYVEWFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPAL 63 AA ++WF EEA R G ++PA V G M +KEPVG VAA TPWNFP + RK++ AL Sbjct: 106 AADTIDWFAEEARRTYGRLVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAAL 165 Query: 64 AAGCTVVCKPAEDTPLTSLALVRLAQEAGVPAGVINIVTASRERTPEVVDVWLADGRVRK 123 AAGC+++ K E+TP + L+R +AGVPAGVIN+V E+ + + ++K Sbjct: 166 AAGCSIIVKAPEETPASPAELIRAYADAGVPAGVINLVYGV---PAEISEYLIPHPVIKK 222 Query: 124 ISFTGSTAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQT 183 ISFTGST VGK LA + +K+ ++ELGG+AP +VFDDADVD A + AKFRN GQ Sbjct: 223 ISFTGSTPVGKQLAALAGQHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQV 282 Query: 184 CVSPNRIYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAI 243 CVSP R VQ+ V++AFVDK LKVG + +GP+ N R I ++ + DA+ Sbjct: 283 CVSPTRFLVQKGVFNAFVDKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAV 342 Query: 244 AGGARVITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVI 303 + GA++ TGGKR+ G+ ++ PTVL ++ + EE FGP+A I F T DEVI Sbjct: 343 SQGAQLRTGGKRIGNKGY----FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVI 398 Query: 304 AAANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREG 363 AN P+GLAAY ++ H + +ESG++ +N LA PFGG+K+SGYG EG Sbjct: 399 TEANRLPYGLAAYAFAKSAHTKHALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEG 458 Query: 364 SVHGLDDYLHTKYVCQ 379 ++ Y++TK V Q Sbjct: 459 GTEAIEAYINTKLVTQ 474 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 279 bits (714), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 160/377 (42%), Positives = 233/377 (61%), Gaps = 9/377 (2%) Query: 4 AASYVEWFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPAL 63 AA +++W EE R G ++ A R + ++EPVGV AAI+PWNFP +K+A A+ Sbjct: 106 AAEHLDWHAEEGRRLYGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAM 165 Query: 64 AAGCTVVCKPAEDTPLTSLALVRLAQEAGVPAGVINIVTA-SRERTPEVVDVWLADGRVR 122 AAGCTVV K ++P + + +L +AGVPAGV NIV+ S E + +++ L VR Sbjct: 166 AAGCTVVLKGPSESPAAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPL----VR 221 Query: 123 KISFTGSTAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQ 182 ISFTGS VG LA +A +K++S+ELGG+AP +VFDDA+V+ A + K RN GQ Sbjct: 222 NISFTGSVPVGSKLAAQAALHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQ 281 Query: 183 TCVSPNRIYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDA 242 CVSP+R YVQ+ +Y FV + +KVGP +P Q+GP+I+ R + + DA Sbjct: 282 VCVSPSRFYVQKGIYARFVTAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDA 341 Query: 243 IAGGARVITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEV 302 +A G+ ++TGG RL G ++YAPT++ V + + EE FGP+A +T F+ +E Sbjct: 342 LAQGSELVTGGARLGQRG----HFYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEA 397 Query: 303 IAAANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGRE 362 IA ANA P GLA+Y ++ + HRV+ L++G+V +N +A PFGG+ ESG+G E Sbjct: 398 IAMANALPLGLASYVFTQSLDTAHRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSE 457 Query: 363 GSVHGLDDYLHTKYVCQ 379 G V G + +L TK + Q Sbjct: 458 GGVEGFESFLVTKMISQ 474 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 243 bits (621), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 144/366 (39%), Positives = 205/366 (56%), Gaps = 10/366 (2%) Query: 15 ATRANGDIIP-APVTGRRMMA--LKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVC 71 A + G IP A G+ ++ ++EP+GVV AI PWN P + + K+A LAAGCTVV Sbjct: 120 ANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVI 179 Query: 72 KPAEDTPLTSLALVRLAQEAGVPAGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTA 131 KPAE+TP ++L L RLAQEAG P GVIN+VT + V KISFTGS Sbjct: 180 KPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRAL---CEHPDVAKISFTGSPE 236 Query: 132 VGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIY 191 G+ + R + + K+++LELGG +P IVFDDA + A+ G F N GQ C + +RI Sbjct: 237 AGRAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRIL 296 Query: 192 VQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIAGGARVIT 251 VQ + F L A + VG + P ++GP+ +++++++ + GA ++ Sbjct: 297 VQRSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLA 356 Query: 252 GGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPF 311 GG G+ + PT+ AD M + EE FGPV I F TE+E IA AN + + Sbjct: 357 GGVSRPDKGW----FVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKY 412 Query: 312 GLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDY 371 GLAA ++T++ R HRV ++ G VGVN + P+GG+K+SG GREG + G Y Sbjct: 413 GLAATVWTTNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAY 472 Query: 372 LHTKYV 377 K V Sbjct: 473 TEEKTV 478 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,267,235 Number of extensions: 55829 Number of successful extensions: 257 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 20 Length of query: 382 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 295 Effective length of database: 1,207,925 Effective search space: 356337875 Effective search space used: 356337875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)