BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0422.p (430 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310459|ref|YP_003774551.1| glycine/D-amino acid oxidase [H... 873 0.0 gi|300309600|ref|YP_003773692.1| glycine/D-amino acid oxidase [H... 457 e-130 gi|300310497|ref|YP_003774589.1| glycine/D-amino acid oxidase [H... 182 6e-47 >gi|300310459|ref|YP_003774551.1| glycine/D-amino acid oxidase [Herbaspirillum seropedicae SmR1] Length = 430 Score = 873 bits (2256), Expect = 0.0, Method: Compositional matrix adjust. Identities = 429/430 (99%), Positives = 429/430 (99%) Query: 1 MKLESYWTDSAPLYISPTRDLPAQVDVAIVGGGFTGLSAALALARRGASVVVLEAGPHVA 60 MKLESYWTDSAPLYISPTRDLPAQVDVAIVGGGFTGLSAALALARRGASVVVLEAGPHVA Sbjct: 1 MKLESYWTDSAPLYISPTRDLPAQVDVAIVGGGFTGLSAALALARRGASVVVLEAGPHVA 60 Query: 61 AEASGRNGGHVNNGLAVDYAELAAKVGVERARAWYHAYDDAVDTVARLVQEEQIDCDFLR 120 AEASGRNGGHVNNGLAVDYAELAAKVGVERARAWYHAYDDAVDTVARLVQEEQIDCDFLR Sbjct: 61 AEASGRNGGHVNNGLAVDYAELAAKVGVERARAWYHAYDDAVDTVARLVQEEQIDCDFLR 120 Query: 121 HGKLKMATRPYQMDALQRSADRLIADGVDLDVEILDAQRVQAEVQSERFHGGLLYKRSGQ 180 HGKLKMATRPYQMDALQRSADRLIADGVDLDVEILDAQRVQAEVQSERFHGGLLYKRSGQ Sbjct: 121 HGKLKMATRPYQMDALQRSADRLIADGVDLDVEILDAQRVQAEVQSERFHGGLLYKRSGQ 180 Query: 181 MHMGRFAYGLAMAAERQGAAIHTGTEVRRLERIGQTHGHRLHTTRGAVLARQVLLATGAS 240 MHMGRFAYGLAMAAERQGAAIHTGTEVRRLERIGQTHGHRLHTTRGAVLARQVLLATGAS Sbjct: 181 MHMGRFAYGLAMAAERQGAAIHTGTEVRRLERIGQTHGHRLHTTRGAVLARQVLLATGAS 240 Query: 241 RHGAYGSFGWLRRRIVPIGSFIVVTEPLGAQRAATLLKHQRTYTTVANIHHYFRMTPDTR 300 RHGAYGSFGWLRRRIVPIGSFIVVTEPLGAQRAATLLKHQRTYTTVANIHHYFRMTPDTR Sbjct: 241 RHGAYGSFGWLRRRIVPIGSFIVVTEPLGAQRAATLLKHQRTYTTVANIHHYFRMTPDTR 300 Query: 301 LVFGGRARFAISSPQSDAASGEVLRRGLHETFPQLGQVRLDYCWGGLVDVTRDRLPQAGE 360 LVFGGRARFAISSPQSDAASGEVLRRGLHETFPQLGQVRLDYCWGGLVDVTRDRLPQAGE Sbjct: 301 LVFGGRARFAISSPQSDAASGEVLRRGLHETFPQLGQVRLDYCWGGLVDVTRDRLPQAGE 360 Query: 361 RDGLYYSMGYSGHGTQMSVHMGQCMAKVMAGDALVNPWREREWPAIPGHFGAPWFLPLVG 420 RDGLYYSMGYSGHGTQMSVHMGQCMAKVMAGDAL NPWREREWPAIPGHFGAPWFLPLVG Sbjct: 361 RDGLYYSMGYSGHGTQMSVHMGQCMAKVMAGDALANPWREREWPAIPGHFGAPWFLPLVG 420 Query: 421 LYYRLKDKLA 430 LYYRLKDKLA Sbjct: 421 LYYRLKDKLA 430 >gi|300309600|ref|YP_003773692.1| glycine/D-amino acid oxidase [Herbaspirillum seropedicae SmR1] Length = 425 Score = 457 bits (1176), Expect = e-130, Method: Compositional matrix adjust. Identities = 241/430 (56%), Positives = 305/430 (70%), Gaps = 8/430 (1%) Query: 1 MKLESYWTDSAPLYISPTRDLP-AQVDVAIVGGGFTGLSAALALARRGASVVVLEAGPHV 59 M+L+SYW D++P ++S T+ LP DV +VGGG TG +AALALAR+GA VVV EA V Sbjct: 2 MQLKSYWLDTSPAFVSQTQTLPEGACDVVVVGGGITGSAAALALARKGARVVVCEADI-V 60 Query: 60 AAEASGRNGGHVNNGLAVDYAELAAKVGVERARAWYHAYDDAVDTVARLVQEEQIDCDFL 119 ASGRNGG NNG A DYA L+ ++GVE A Y A+D VDTV RLV+EE IDC F Sbjct: 61 GGAASGRNGGMCNNGFAQDYATLSQRLGVEWANRLYRAFDAGVDTVERLVREESIDCSFA 120 Query: 120 RHGKLKMATRPYQMDALQRSADRLIADGVDLDVEILDAQRVQAEVQSERFHGGLLYKRSG 179 RHGKLK+A +P +D L RS + L+A VD D +L +++ E+ S+R+HGGLL +S Sbjct: 121 RHGKLKLAAKPEHVDKLARSQE-LLARHVDGDTRLLSRAQLRDELGSDRYHGGLLMHKSA 179 Query: 180 QMHMGRFAYGLAMAAERQGAAIHTGTEVRRLERIGQTHGHRLHTTRGAVLARQVLLATGA 239 MH+GR+ GLA AA+R+GA T V +E + + + T+ G + A QVLLA+G Sbjct: 180 GMHVGRYVRGLAQAAQRRGALYLERTPVLGIE--AKNGRYAVRTSAGVLHAGQVLLASGI 237 Query: 240 SRHGAYGSFGWLRRRIVPIGSFIVVTEPLGAQRAATLLKHQRTYTTVANIHHYFRMTPDT 299 S+ G FGW+RRRIVP+G+F++VTEPL LL QR YT N +YFR TPD Sbjct: 238 SQ---VGPFGWIRRRIVPVGAFLIVTEPLPGALLKQLLPTQRMYTDTKNFVNYFRATPDQ 294 Query: 300 RLVFGGRARFAISSPQSDAASGEVLRRGLHETFPQLGQVRLDYCWGGLVDVTRDRLPQAG 359 RL+FGGRARFA S+PQSDA SGE+LR + E FP L Q R+DYCWGG+VD+T DRLP+AG Sbjct: 295 RLLFGGRARFAASNPQSDARSGEILRAQMLEVFPALAQTRIDYCWGGMVDMTTDRLPRAG 354 Query: 360 ERDGLYYSMGYSGHGTQMSVHMGQCMAKVMAGDALVNPWREREWPAIPGHFGAPWFLPLV 419 +RDGLYYSMGYSGHGT M+ MG MA++M G A +NPW++ +WPAIPGHFG PWFLP V Sbjct: 355 QRDGLYYSMGYSGHGTHMATLMGSMMAEIMDGHAELNPWKDFDWPAIPGHFGKPWFLPFV 414 Query: 420 GLYYRLKDKL 429 G +YRLKD+L Sbjct: 415 GAWYRLKDRL 424 >gi|300310497|ref|YP_003774589.1| glycine/D-amino acid oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 182 bits (461), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 143/398 (35%), Positives = 196/398 (49%), Gaps = 23/398 (5%) Query: 5 SYWTDSAPLYI-SPTRDLPAQVDVAIVGGGFTGLSAALALARRGASVVVLEAGPHVAA-E 62 S W +A + +P A+ DVAIVG G+TGLS AL LA+ G SV VL+A H Sbjct: 12 SLWAATAGEFSGAPPLQASARYDVAIVGAGYTGLSTALHLAQAGVSVCVLDA--HAPGWG 69 Query: 63 ASGRNGGHVNNGLAVDYAELAAKVGVERARAWYHAYDDAVDTVARLVQEEQIDCDFLRHG 122 ASGRNGG V GL D +L G A A A DTV L+ I+C+ R G Sbjct: 70 ASGRNGGQVIPGLKYDPDQLRVMFGSAVADPLIAAIGSAADTVFDLIARHGIECEAQRAG 129 Query: 123 KLKMATRPYQMDALQRSADRLIADGVDLDVEILDAQRVQAEVQSERFHGGLLYKRSGQMH 182 ++ P M AL+ A + +A+G +++D V + + + GG +R+G +H Sbjct: 130 WIQPTHSPKMMRALEARARQWMAEGA--PAQLMDGAEVTRRIGTGAYVGGWKDERAGSLH 187 Query: 183 MGRFAYGLAMAAERQGAAIHTGTEVRRLERIGQTHGHRLHTTRGA----VLARQVLLATG 238 ++ GLA AA+R G IH T V RLER + G RLH G + A +V+LAT Sbjct: 188 PLKYCRGLAQAAQRLGVVIHGDTRVTRLER--RQGGWRLHGPSGEQGPHIDAERVVLAT- 244 Query: 239 ASRHGAYGSFGW--LRRRIVPIGSFIVVTEPLGAQRAATLLKHQRTYTTVANIHHYFRMT 296 Y W LR+ ++ SFIV T PL A++L + + Y+R Sbjct: 245 ----NGYTDDLWPHLRQSVIAANSFIVATRPLPPALGASILPGGEVTSDSRRLLLYYRRD 300 Query: 297 PDTRLVFGGRARFAISSPQSDAASGEVLRRGLHETFPQLGQVRLDYCWGGLVDVTRDRLP 356 RL+ GGR F +D A L R + FPQL V +Y W G V +TRD LP Sbjct: 301 AQGRLLMGGRGPFGEPQGPADFAH---LERSVALLFPQLAGVEYEYRWSGRVAITRDFLP 357 Query: 357 QAGER-DGLYYSMGYSGHGTQMSVHMGQCMAKVMAGDA 393 E GL ++GY+G G M+ MGQ +A+ + G A Sbjct: 358 HVHEPAPGLSIALGYNGRGIAMATMMGQRLAQRLIGPA 395 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,418,306 Number of extensions: 60666 Number of successful extensions: 272 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 263 Number of HSP's successfully gapped: 3 Length of query: 430 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 342 Effective length of database: 1,203,190 Effective search space: 411490980 Effective search space used: 411490980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)