BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0421.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogen... 619 e-179 gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid... 153 2e-38 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 83 3e-17 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 76 3e-15 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 74 1e-14 >gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 619 bits (1596), Expect = e-179, Method: Compositional matrix adjust. Identities = 307/308 (99%), Positives = 308/308 (100%) Query: 1 MTFLYKADPVRGAEWAQLFAQKAPELPFQIWPHDGDGASVRYLAAWTPPDDIAERFPKLE 60 MTFLYKADPVRGAEWAQLFAQKAPELPFQIWPHDGDGASVRYLAAWTPPDDIAERFPKLE Sbjct: 1 MTFLYKADPVRGAEWAQLFAQKAPELPFQIWPHDGDGASVRYLAAWTPPDDIAERFPKLE 60 Query: 61 ILFSVGAGIDQFDMSTLPPALPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRVYARQQRDQ 120 ILFSVGAGIDQFDMSTLPPALPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRVYARQQRDQ Sbjct: 61 ILFSVGAGIDQFDMSTLPPALPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRVYARQQRDQ 120 Query: 121 VWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDCYSGSAGL 180 VWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDCYSGSAGL Sbjct: 121 VWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDCYSGSAGL 180 Query: 181 RAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHLVEDDLLAALNDGQ 240 RAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHLVEDDLLAALNDGQ Sbjct: 181 RAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHLVEDDLLAALNDGQ 240 Query: 241 LSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRHREGLPLLG 300 LSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRR+REGLPLLG Sbjct: 241 LSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRYREGLPLLG 300 Query: 301 LIDRQRGY 308 LIDRQRGY Sbjct: 301 LIDRQRGY 308 >gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 153 bits (386), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 107/255 (41%), Positives = 142/255 (55%) Query: 54 ERFPKLEILFSVGAGIDQFDMSTLPPALPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRVY 113 +R P L + SV AG+++ L + R+ +P +AA M + V L LHRD +Y Sbjct: 65 KRMPNLLWVHSVWAGVERLVADLGQSDLQLTRLVDPRLAATMAEAVLAWTLYLHRDMPLY 124 Query: 114 ARQQRDQVWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDC 173 AR+QR+ W A RV +LGLG LG A L F GWSRSA + +G+ Sbjct: 125 ARRQREHHWEAQPYVLPEQRRVSLLGLGALGEAAAAALRQARFAVNGWSRSAREAEGVQT 184 Query: 174 YSGSAGLRAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHLVEDDLL 233 + G AGL LA+TD+LVCL+PLT ETRGL+ A LP A LIN RG + + L Sbjct: 185 FHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPIIDDAALK 244 Query: 234 AALNDGQLSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRHR 293 AAL+ GQL AVLDV VEPLPP W+HP + + PH ++ T A V N+RR+R Sbjct: 245 AALDAGQLRHAVLDVFAVEPLPPAAWPWSHPAVTVLPHCSAPTDRQTASAIVAANIRRYR 304 Query: 294 EGLPLLGLIDRQRGY 308 + +D +RGY Sbjct: 305 HSGEIAANVDLRRGY 319 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 82.8 bits (203), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 64/230 (27%), Positives = 99/230 (43%), Gaps = 6/230 (2%) Query: 54 ERFPKLEILFSVGAGIDQFDMSTLPPALPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRVY 113 E P+L+I+ ++ G+D FD+ ++ T + + +L R Sbjct: 62 EAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARRVVEL 121 Query: 114 ARQQRDQVWRAHRVRPASACRV-----GVLGLGMLGRAVLQQLH-GLGFPCAGWSRSAHQ 167 A + W+ V G++G+G +G AV ++ H G G P +R Sbjct: 122 AEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPDP 181 Query: 168 MDGIDCYSGSAGLRAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHL 227 + + LA+ D + ++PLT +T + FA + R A IN RG + Sbjct: 182 EAERELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRIV 241 Query: 228 VEDDLLAALNDGQLSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMTQ 277 E L+AAL D + A LDV EVEPLP P P++V PHI S T Sbjct: 242 DEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATH 291 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 76.3 bits (186), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 72/242 (29%), Positives = 116/242 (47%), Gaps = 9/242 (3%) Query: 56 FPKLEILFSVGAGIDQFDMSTLPPALPVVRMTEPGIAAGMV-QYVTHAVLGLHRDSRVYA 114 P L+++ ++GAG + D++ A + T G V + +L + R+ V Sbjct: 64 LPHLQLVCTLGAGFENVDVAH-AEAHGIEIATGAGTNEDCVADHALGLLLAILRNIPVLD 122 Query: 115 RQQRDQVWRAHR-VRPASAC-RVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGID 172 R RD WR ++P A RVG++G+G +G+ + ++ A +R + D +D Sbjct: 123 RYTRDGGWRETIPLQPQLAGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNRK--KRDDVD 180 Query: 173 CYSGSAGLRAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHLVEDDL 232 Y + D L+ P +TR L+ + +L L+N+GRG + D L Sbjct: 181 -YHYFPDVAQLAGWADCLIVAAPGGAQTRHLINARVLEELGPQGYLVNIGRGSIVDTDAL 239 Query: 233 LAALNDGQLSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRH 292 AAL+ G+L+ A LDV E EP PP P++VLTPHIA + P+ +V + LR Sbjct: 240 GAALSSGRLAGAGLDVYEGEPQPPA-ALIALPNVVLTPHIAGWS-PEAIRASVTQFLRNC 297 Query: 293 RE 294 E Sbjct: 298 EE 299 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 74.3 bits (181), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 54/167 (32%), Positives = 82/167 (49%), Gaps = 11/167 (6%) Query: 115 RQQRDQVW--RAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAG---WSRSAHQMD 169 ++ R+ W H+ +G++GLG +GR V LG P +++ A Q Sbjct: 127 QRMREGHWDKSTHKSLELQGRTLGLVGLGAIGRRVAAVAAALGMPVLAHDPYAKEAPQ-- 184 Query: 170 GIDCYSGSAGLRAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHLVE 229 G+ L A++DV+ PLT E + ++ A++ GA L+N RGG + E Sbjct: 185 GVQLVD----LDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDE 240 Query: 230 DDLLAALNDGQLSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMT 276 L+AAL+ G+L AA LD E EP HP + VL+PHI +T Sbjct: 241 QALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVT 287 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.141 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,098,863 Number of extensions: 49107 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 11 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)