BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0407.p (487 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 951 0.0 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 291 8e-80 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 288 5e-79 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 288 1e-78 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 266 2e-72 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 951 bits (2457), Expect = 0.0, Method: Compositional matrix adjust. Identities = 475/487 (97%), Positives = 478/487 (98%), Gaps = 1/487 (0%) Query: 1 MTSSPLTHLTRNARLDHFYIDGKWQPPQGGERAAVVAPATEERVCEIALGNAADVDQAVQ 60 MTSSPLTHLTRNARLDHFYIDGKWQPPQG ERAAVVAPATEERVCEIALGNAADVDQAVQ Sbjct: 1 MTSSPLTHLTRNARLDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQ 60 Query: 61 AARRAIAKHTPQPTPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAA 120 AARRAI +PLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAA Sbjct: 61 AARRAIPAWAAT-SPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAA 119 Query: 121 EHLRVARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVV 180 EHLRVARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVV Sbjct: 120 EHLRVARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVV 179 Query: 181 LKPSELSPLSALLFAEIVHEAGVPAGVFNLVNGNGAEVGAALSAHPQVDMISITGSTRAG 240 LKPSELSPLSALLFAEIVHEAGVPAGVFNLVNG+GAEVGAALSAHPQVDMISITGSTRAG Sbjct: 180 LKPSELSPLSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAG 239 Query: 241 VLVAQAAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVP 300 VLVAQAAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVP Sbjct: 240 VLVAQAAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVP 299 Query: 301 RAALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGG 360 RAALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGG Sbjct: 300 RAALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGG 359 Query: 361 PGRPDGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLG 420 PGRPDGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLG Sbjct: 360 PGRPDGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLG 419 Query: 421 AHVQGSDKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEYLEVK 480 AHVQG DKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEY+EVK Sbjct: 420 AHVQGRDKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVK 479 Query: 481 AILGYYA 487 AILGYYA Sbjct: 480 AILGYYA 486 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 291 bits (745), Expect = 8e-80, Method: Compositional matrix adjust. Identities = 175/470 (37%), Positives = 260/470 (55%), Gaps = 15/470 (3%) Query: 19 YIDGKWQPPQGGERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIAKHTPQPTPLER 78 +++G+W GER AV PA E + + L AA+ +A++AA A + T ER Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKK-TAKER 73 Query: 79 AALLGRVHALLLERSELFAQALTLEMGAP-------ISYARTAHVPLAAEHLRVARDNLA 131 +A+L R + L+L ++ A +T E G P I YA + A E R D + Sbjct: 74 SAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI- 132 Query: 132 DYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSA 191 P P +++E IGVCA ITPWN+P IT K GPALAAGC +VLKP+E +P SA Sbjct: 133 --PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSA 190 Query: 192 LLFAEIVHEAGVPAGVFNLVNGNGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTV 251 L A + AG+PAGVF++V G +G ++++P V IS TGST G L+ Q +A ++ Sbjct: 191 LALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSI 250 Query: 252 KRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELA 311 K+++ ELGG +P +V DADL+ A+ +A+ RN GQ+C R+ V Sbjct: 251 KKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKL 310 Query: 312 LATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGY 371 + + + KVG + T GPL N +V+Q + + GAR+L G G+ L + Sbjct: 311 VEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLG--GKRHALGHSF 368 Query: 372 YARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGSDKDQV 431 + PT+ ++V M +A+EE FGP+ + + T EE +A+ANDT +GL ++ D ++ Sbjct: 369 F-EPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRI 427 Query: 432 RAVAARIQSGQVHLNYPAWDPQ-APFGGYKQSGNGREYGVEGMEEYLEVK 480 VA ++SG V +N + APFGG KQSG GRE G+++YL VK Sbjct: 428 WRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVK 477 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 288 bits (738), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 195/478 (40%), Positives = 260/478 (54%), Gaps = 14/478 (2%) Query: 15 LDHFYIDGKWQPPQGGERAAVVAPATEERVCEIALGNAADVDQAVQAARRAI-AKHTPQP 73 L HF I G+ G+R +V P EE A G AADV +AV AA+ + + Sbjct: 5 LQHF-IAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKL 63 Query: 74 TPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVARD----- 128 + L+R LL ++ AL+ ++L A +G R VP A HLR A Sbjct: 64 SGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQM 123 Query: 129 ---NLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSE 185 + ++ + VREPIGV I PWN P+ + KV LAAGCTVV+KP+E Sbjct: 124 EGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAE 183 Query: 186 LSPLSALLFAEIVHEAGVPAGVFNLVNGNGAEVGAALSAHPQVDMISITGSTRAGVLVAQ 245 +P SAL A + EAG P GV N+V G G VG AL HP V IS TGS AG + + Sbjct: 184 ETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQR 243 Query: 246 AAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALA 305 A KRV ELGGKSP +V DA E A+ N GQ C+A +R++V R+ Sbjct: 244 IAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQ 303 Query: 306 RVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPD 365 R + A + VGDP+ GP+A +AQF RV + I+ GL +GA LLAGG RPD Sbjct: 304 RFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPD 363 Query: 366 GLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQG 425 +G++ +PTIF++ + M IA+EEIFGPV I+ +DT EEAIA+AND+ YGL A V Sbjct: 364 ---KGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWT 420 Query: 426 SDKDQVRAVAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYLEVKAI 482 ++ + VAA ++ G V +N + D P+GG K SG GRE G+ G Y E K + Sbjct: 421 TNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTV 478 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 288 bits (736), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 186/487 (38%), Positives = 265/487 (54%), Gaps = 18/487 (3%) Query: 14 RLDHFYIDGKWQPPQGGERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIAKHTPQP 73 R D+F I GK+ PP GE ++P CE+A +A DV+ A+ AA A K + Sbjct: 18 RYDNF-IGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAA-KKSWGKT 75 Query: 74 TPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRV----ARDN 129 +P ERA +L ++ + EL A A TL+ G PI A +PLA +H R R Sbjct: 76 SPTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQ 135 Query: 130 LADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPL 189 I EP+GV I PWN+PI K+ PALAAG VVLKP+E +P Sbjct: 136 EGSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPA 195 Query: 190 SALLFAEIVHEAGVPAGVFNLVNGNGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAV 249 S ++ E++ + +P GV N+V G G E G L+++ ++ I+ TG T G L+ Q A+ Sbjct: 196 SIMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQ 254 Query: 250 TVKRVAQELGGKSPNLVLPDA-----DLERAIPPGVAAALRNMGQSCSAPTRLIVPRAAL 304 + V ELGGKSPN+ D D G A N G+ C+ P+R++V + Sbjct: 255 NLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESIY 314 Query: 305 ARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRP 364 R E AL +A +K G+P D +T G A++ Q ++ I+ G Q+GA++LAGG GR Sbjct: 315 ERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG-GRE 373 Query: 365 D---GLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGA 421 + LA GYY +PT+F + + M I QEEIFGPV+++ + EEA+AIANDT+YGLGA Sbjct: 374 ELGGDLASGYYVKPTVF-QGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGA 432 Query: 422 HVQGSDKDQVRAVAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYLEVK 480 + D + + IQ+G+V N Y + A FGGYKQSG GRE ++ Y + K Sbjct: 433 GLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTK 492 Query: 481 AILGYYA 487 +L Y+ Sbjct: 493 NLLVSYS 499 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 266 bits (681), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 164/473 (34%), Positives = 250/473 (52%), Gaps = 13/473 (2%) Query: 17 HFYIDGKWQPPQGGERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIAKHTPQPTPL 76 +I+G+W G V+ PATEE + +IA + AD+D+A++AA + + Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNM-SAF 64 Query: 77 ERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLA------AEHLRVARDNL 130 ER+ ++ R LL ER+E A+ +T+E G P++ A+ + A AE R L Sbjct: 65 ERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRL 124 Query: 131 ADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLS 190 P P +++EP+G A TPWN+P+ Q+ K+ ALAAGC++++K E +P S Sbjct: 125 V--PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPAS 182 Query: 191 ALLFAEIVHEAGVPAGVFNLVNGNGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVT 250 +AGVPAGV NLV G AE+ L HP + IS TGST G +A A Sbjct: 183 PAELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQH 242 Query: 251 VKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHEL 310 +KR ELGG +P +V DAD++ A A RN GQ C +PTR +V + + Sbjct: 243 MKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDK 302 Query: 311 ALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQG 370 + Q+KVG+ +A T GP+AN+ + ++ +I+ + GA+L GG + +G Sbjct: 303 FVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGN---KG 359 Query: 371 YYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGSDKDQ 430 Y+ +PT+ + + EE FGP+ I ++T++E I AN YGL A+ Sbjct: 360 YFFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHT 419 Query: 431 VRAVAARIQSGQVHLNYPAWD-PQAPFGGYKQSGNGREYGVEGMEEYLEVKAI 482 A+AAR++SG + +N+ P+ PFGG K SG G E G E +E Y+ K + Sbjct: 420 KHALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLV 472 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,664,104 Number of extensions: 75265 Number of successful extensions: 389 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 307 Number of HSP's successfully gapped: 21 Length of query: 487 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 398 Effective length of database: 1,198,455 Effective search space: 476985090 Effective search space used: 476985090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)