BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0406.p (402 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 149 4e-37 gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 61 1e-10 gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 61 2e-10 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 149 bits (376), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 67/67 (100%), Positives = 67/67 (100%) Query: 1 MEHTGHQHDTLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGT 60 MEHTGHQHDTLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGT Sbjct: 1 MEHTGHQHDTLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGT 60 Query: 61 GVVASLA 67 GVVASLA Sbjct: 61 GVVASLA 67 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 35/75 (46%), Positives = 44/75 (58%), Gaps = 2/75 (2%) Query: 3 HTGH--QHDTLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGT 60 H GH + LH + +RR LH HPEL FEE TA +V + L+ GY VTR +GG Sbjct: 5 HPGHLCEVGDLHDIQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGN 64 Query: 61 GVVASLAVTRSAASC 75 G+VA+L V SA S Sbjct: 65 GLVATLRVGSSARSI 79 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 61.2 bits (147), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 29/46 (63%), Positives = 33/46 (71%) Query: 21 LRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASL 66 +RR LHAHPELRFEE RTA V L+S G TV +GG GVVA+L Sbjct: 20 IRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATL 65 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.131 0.468 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 622,174 Number of extensions: 12330 Number of successful extensions: 129 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 129 Number of HSP's successfully gapped: 3 Length of query: 402 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 315 Effective length of database: 1,207,925 Effective search space: 380496375 Effective search space used: 380496375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 132 (55.5 bits)