BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0404.p (515 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 1019 0.0 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 667 0.0 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 523 e-149 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 488 e-139 gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sen... 471 e-134 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 1019 bits (2636), Expect = 0.0, Method: Compositional matrix adjust. Identities = 514/515 (99%), Positives = 515/515 (100%) Query: 1 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ 60 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ Sbjct: 1 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ 60 Query: 61 GSLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL 120 GSLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL Sbjct: 61 GSLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL 120 Query: 121 KNLTAYVKVDEQLAEMAANQQYDETRALFNGESAKLYRSMVGNLDAIVKINTSGSAQADE 180 KNLTAYVKVDEQLAEMAANQQYDETRALFNG+SAKLYRSMVGNLDAIVKINTSGSAQADE Sbjct: 121 KNLTAYVKVDEQLAEMAANQQYDETRALFNGDSAKLYRSMVGNLDAIVKINTSGSAQADE 180 Query: 181 EASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRP 240 EASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRP Sbjct: 181 EASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRP 240 Query: 241 AGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSLE 300 AGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSLE Sbjct: 241 AGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSLE 300 Query: 301 ETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIAD 360 ETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIAD Sbjct: 301 ETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIAD 360 Query: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIDD 420 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIDD Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIDD 420 Query: 421 SVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMDET 480 SVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMDET Sbjct: 421 SVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMDET 480 Query: 481 TQQNAALVEQAAAAADSLKGQASNLAAVVGKFKLD 515 TQQNAALVEQAAAAADSLKGQASNLAAVVGKFKLD Sbjct: 481 TQQNAALVEQAAAAADSLKGQASNLAAVVGKFKLD 515 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 667 bits (1722), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/514 (69%), Positives = 426/514 (82%) Query: 1 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ 60 M WF NL IAKKL +SFA VI LT++ GLFAI+ELK VN+AS ++ + W+PT+ AA+ +Q Sbjct: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 Query: 61 GSLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL 120 SLP +R++EL++VTA TP ER A KAIK RLD L +QRA +E ++SE +EK Y +F Sbjct: 61 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS 120 Query: 121 KNLTAYVKVDEQLAEMAANQQYDETRALFNGESAKLYRSMVGNLDAIVKINTSGSAQADE 180 K+ Y+++D++LA ++A + +E RALF G+S K +RSM+ N+D I+K+N +GS ++D+ Sbjct: 121 KSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 Query: 181 EASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRP 240 A V+R A WI+GLLAA V+IA LA +VA VSRPL EAV +A+RVA+GDLT+ IR Sbjct: 181 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRA 240 Query: 241 AGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSLE 300 DETG LM +LRAMN+SL+ IV EVR GTDTITTAS EIA GNLDLS+RTE+QAGSLE Sbjct: 241 TSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAGSLE 300 Query: 301 ETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIAD 360 ETASAMEELT+TV QNADNARQANQLAA+ASDIA+QGG VVGQVVQTMEGITESS RIAD Sbjct: 301 ETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSNRIAD 360 Query: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIDD 420 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA AAKEIK+LIDD Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDD 420 Query: 421 SVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMDET 480 SV+KV AGS LVERAG+TM+EVVASVRRVTDVVAEISAA+ EQS GI E+N AITQMDE Sbjct: 421 SVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINRAITQMDEV 480 Query: 481 TQQNAALVEQAAAAADSLKGQASNLAAVVGKFKL 514 TQQNAALVE+AAAAA SL+ QA+ L+ VV FKL Sbjct: 481 TQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKL 514 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 523 bits (1347), Expect = e-149, Method: Compositional matrix adjust. Identities = 297/515 (57%), Positives = 381/515 (73%) Query: 1 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ 60 M WFY+L I++KL +F VV+A+ G+F+I++L QVN AS EI + W+P++ LQ Sbjct: 1 MKWFYDLRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQ 60 Query: 61 GSLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL 120 ++ LR EL+ + A +E + ++ +++ L +A + ++SEP+EK Y + Sbjct: 61 LAMSRLRSFELQHILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEVE 120 Query: 121 KNLTAYVKVDEQLAEMAANQQYDETRALFNGESAKLYRSMVGNLDAIVKINTSGSAQADE 180 K + +V +++ ++ + +E R L GES K YR+ + +D + +N G+ +++ Sbjct: 121 KLIATFVSEHDKIIALSRQGKDEEARNLQRGESTKAYRTSLQQIDKLAAVNDKGADISNK 180 Query: 181 EASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRP 240 +A ++ A+ WI+ +AA + +A LLA V+ ++RPL+EAV +AQRVA GDLT RI P Sbjct: 181 QADAIYAQARLWIIVTVAACIGLAMLLAVWVSRLIARPLEEAVSVAQRVAGGDLTTRIEP 240 Query: 241 AGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSLE 300 AG+DETG L+ AL++MN+SL IV +VR GT TITTAS EIA GNLDLS+RTE+QA SLE Sbjct: 241 AGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLSSRTEEQASSLE 300 Query: 301 ETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIAD 360 ETASAMEELTSTV QNADNARQANQLA +AS++ASQGG VV QVV TM I SSR+IAD Sbjct: 301 ETASAMEELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVDTMGSINASSRKIAD 360 Query: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIDD 420 II VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELI+ Sbjct: 361 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIEG 420 Query: 421 SVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMDET 480 SV +V GS LVERAG TM+EVV SVRRVTD+V EIS+A+QEQS GI +VNLAI QMDE Sbjct: 421 SVAQVDMGSKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQSEGIEQVNLAIAQMDEV 480 Query: 481 TQQNAALVEQAAAAADSLKGQASNLAAVVGKFKLD 515 TQQNAALVE+AAAAA SL+ QA L VG FKL+ Sbjct: 481 TQQNAALVEEAAAAAQSLQEQAETLTQTVGFFKLE 515 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 488 bits (1257), Expect = e-139, Method: Compositional matrix adjust. Identities = 285/516 (55%), Positives = 369/516 (71%), Gaps = 2/516 (0%) Query: 1 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ 60 M WFYNL IA KL +F VV+ + G F+ EL VNK+S +I W+P++DA ++ Sbjct: 1 MNWFYNLKIAHKLNAAFLVVLVFIIGLGGFSSLELAAVNKSSTDIARNWLPSIDALSKMS 60 Query: 61 GSLPPLRLNELE-LVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQF 119 +L R +++ L+TA +E+A+ +A K ++ L ++ A + + +SEP+EKE Y + Sbjct: 61 LALARSRSFDMQQLLTADKQEEKASRERADK-QMATLLKEVARYGKLVSEPREKELYPEI 119 Query: 120 LKNLTAYVKVDEQLAEMAANQQYDETRALFNGESAKLYRSMVGNLDAIVKINTSGSAQAD 179 +N+ Y + E+++ + Q AL N S +YR + + + ++N+ G+A +D Sbjct: 120 ERNIALYKEAHERMSALFEQDQKQAAHALMNSTSTPVYRKLQEQIGELSEVNSKGAALSD 179 Query: 180 EEASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIR 239 +A ++ A I+ L+ V++A L++ +A V+RPL AV IA++VA GDLT I Sbjct: 180 ADADHIYGQANMAILALVTTCVVLAMALSWWIARLVARPLANAVDIARQVAAGDLTADIP 239 Query: 240 PAGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSL 299 A RDETG L+EALR MN +L +IV EVRQGTDTITTAS EIA GN+DLS+RTEQQAG+L Sbjct: 240 TAHRDETGQLLEALRQMNGNLLNIVREVRQGTDTITTASAEIATGNMDLSSRTEQQAGAL 299 Query: 300 EETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIA 359 EETASAMEELTSTV QN+DNARQANQLA AS++A GG VVG+VV TM I +SSR+I Sbjct: 300 EETASAMEELTSTVRQNSDNARQANQLAVNASEVAQAGGTVVGKVVATMSAINDSSRKIV 359 Query: 360 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELID 419 DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA AA EIK LID Sbjct: 360 DIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAHEIKALID 419 Query: 420 DSVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMDE 479 DSV KV GS LV+ AG TM EVVASV+RV+D+VAEI+AA EQ+ GI ++N A+ QMDE Sbjct: 420 DSVAKVDTGSKLVDEAGQTMNEVVASVKRVSDIVAEITAAGAEQTQGIEQINNAVVQMDE 479 Query: 480 TTQQNAALVEQAAAAADSLKGQASNLAAVVGKFKLD 515 TQQNAALVEQAAAAA +L+ QA L+ V F+LD Sbjct: 480 NTQQNAALVEQAAAAAKALQEQAGRLSETVSVFRLD 515 >gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 471 bits (1213), Expect = e-134, Method: Compositional matrix adjust. Identities = 272/516 (52%), Positives = 366/516 (70%), Gaps = 3/516 (0%) Query: 1 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ 60 M WFYN IA KL L+FA V+ LT G+F+I +L +VN+A+ E+ + W+P++ + +++ Sbjct: 1 MNWFYNRRIATKLLLAFACVLLLTGGLGIFSIVQLHKVNQAATELATNWLPSIRSLNDMK 60 Query: 61 GSLPPLRLNELELVTAVTPQERAAANKAIKDR-LDALTRQRAIFEQRMSEPQEKEQYAQF 119 +L +R N+ +L +++++A + ++ L+AL R + ++SEP EK + Sbjct: 61 VALSRVRANQAQLALYDGDEKQSSAVIGLMNKNLEALAEARKQYTGQISEPGEKALFPGV 120 Query: 120 LKNLTAYVKVD-EQLAEMAANQQYDETRALFNGESAKLYRSMVGNLDAIVKINTSGSAQA 178 + ++ + + LA + A + DE + L G + + Y S++ +LD + ++N GS Q+ Sbjct: 121 NARIDKFIALHGDTLAAVRAGK-LDEAKRLLLGPAFENYLSLLNDLDKLAQVNLDGSVQS 179 Query: 179 DEEASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRI 238 A T + AQ W+V +L +L LA +A ++ PL+ A + A +A GDLT I Sbjct: 180 TRSAETTYDQAQVWVVAILIGALLAGLALALFIARVIATPLRSAGEFAHLIAAGDLTADI 239 Query: 239 RPAGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGS 298 DETG L AL AMN SLR IVS+VR GTD I TAS +I+ GNLDLS+RTE+QA S Sbjct: 240 TARSDDETGQLTAALAAMNTSLRRIVSQVRSGTDAIATASGQISSGNLDLSSRTEEQASS 299 Query: 299 LEETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRI 358 LEETASAMEELTSTV QNA+NARQANQLA +AS IA +GG+VV QV++TM I SS++I Sbjct: 300 LEETASAMEELTSTVRQNAENARQANQLALSASTIAEEGGKVVSQVIETMGSIDASSKKI 359 Query: 359 ADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELI 418 DIISVID IAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA AAKEIK LI Sbjct: 360 VDIISVIDSIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSATAAKEIKTLI 419 Query: 419 DDSVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMD 478 D+SVQ+V GS LV++AG T++EVV+SV+RVTDV++EISAA+ EQ+ GI +VN AITQMD Sbjct: 420 DNSVQQVGLGSGLVQKAGVTISEVVSSVQRVTDVMSEISAASGEQTQGIEQVNQAITQMD 479 Query: 479 ETTQQNAALVEQAAAAADSLKGQASNLAAVVGKFKL 514 E TQQNAALVE+AA+A+ SL QA L+ +VG F+L Sbjct: 480 EVTQQNAALVEEAASASQSLSHQAQRLSELVGVFRL 515 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.125 0.321 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,120,909 Number of extensions: 37867 Number of successful extensions: 492 Number of sequences better than 1.0e-08: 69 Number of HSP's gapped: 268 Number of HSP's successfully gapped: 70 Length of query: 515 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 426 Effective length of database: 1,198,455 Effective search space: 510541830 Effective search space used: 510541830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)