BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0401.p (409 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 792 0.0 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 561 e-161 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 286 3e-78 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 285 4e-78 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 284 9e-78 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 792 bits (2046), Expect = 0.0, Method: Compositional matrix adjust. Identities = 398/400 (99%), Positives = 398/400 (99%) Query: 10 IDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMA 69 IDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMA Sbjct: 50 IDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMA 109 Query: 70 TWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGS 129 TWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGS Sbjct: 110 TWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGS 169 Query: 130 ALASILSGPISGGLLQIDGGGLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQ 189 ALASILSGPISGGLLQIDGGGL GWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQ Sbjct: 170 ALASILSGPISGGLLQIDGGGLQGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQ 229 Query: 190 ERSAITSAIVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSII 249 ERSAIT AIVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSII Sbjct: 230 ERSAITRAIVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSII 289 Query: 250 RKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFG 309 RKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFG Sbjct: 290 RKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFG 349 Query: 310 NSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFGF 369 NSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFGF Sbjct: 350 NSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFGF 409 Query: 370 LEQRTGSIQGGLMGLAITSLVAAGVVFLARSGPGATRRPR 409 LEQRTGSIQGGLMGLAITSLVAAGVVFLARSGPGATRRPR Sbjct: 410 LEQRTGSIQGGLMGLAITSLVAAGVVFLARSGPGATRRPR 449 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 561 bits (1445), Expect = e-161, Method: Compositional matrix adjust. Identities = 284/395 (71%), Positives = 331/395 (83%) Query: 10 IDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMA 69 IDRVNIGFVRSHL+TD+GIG AA+GLGAGLFFVGYALFEVPSN+LLQR+GA+AWLTRIM Sbjct: 38 IDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYALFEVPSNMLLQRYGARAWLTRIMF 97 Query: 70 TWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGS 129 TWGL A MAFV SET+FY +RFLLG AEAGFFPGV+YYFTQWLP ERGRAMA+FLSGS Sbjct: 98 TWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGS 157 Query: 130 ALASILSGPISGGLLQIDGGGLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQ 189 A+ASILSGPI+G LL I+GGGLHGWQWMFIIEG AS++ C VW LDS P +AKWL+ + Sbjct: 158 AIASILSGPITGALLLIEGGGLHGWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQE 217 Query: 190 ERSAITSAIVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSII 249 +R A+ + I EQ +R ++PA VS LLRDPQI++FCFIYF+I LTIYGATFWLP+II Sbjct: 218 QREALAAVIAAEQEQRAANRPAHVSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTII 277 Query: 250 RKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFG 309 +KMG F+DFQVGLFNSIPW+++I AMY FA LAAR+K Q W AT LVI+AAGM S Sbjct: 278 KKMGHFNDFQVGLFNSIPWIVAIAAMYLFAVLAARYKKPQFWVATTLVISAAGMAISAVA 337 Query: 310 NSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFGF 369 V FV++CFA +GFKAASSLFWP+PQAYLDARIAA V+ALINS+GNLGGF AP FG+ Sbjct: 338 GPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGY 397 Query: 370 LEQRTGSIQGGLMGLAITSLVAAGVVFLARSGPGA 404 LEQ+TGSIQGGL L+ SL+AA +VFLAR+ P A Sbjct: 398 LEQKTGSIQGGLFALSGASLLAAVLVFLARTRPAA 432 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 286 bits (731), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 154/403 (38%), Positives = 237/403 (58%), Gaps = 12/403 (2%) Query: 10 IDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMA 69 +DRVN+GF + ++ D+G +GLGAGLFF+GY LFEVPSN+L+ + GA+ W+ RIM Sbjct: 42 LDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMI 101 Query: 70 TWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGS 129 TWG+ + A FV + T FY+LRFLLG AEAGF+PG+I Y T W P+ R + +A+F+SG Sbjct: 102 TWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGI 161 Query: 130 ALASILSGPISGGLLQI--DGGGLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLT 187 +A IL P+SG ++ GGL GWQWMF+IE + +VL+ +LD+ AKWL Sbjct: 162 PVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLN 221 Query: 188 AQERSAITSAIV-DEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLP 246 +E++++ + I D + + KH + ++D ++ + C IYFS + YG T W+P Sbjct: 222 DEEKASLQADIDGDAKGKESKHGFGAI-----VKDARVWLMCLIYFSFVMGQYGLTLWMP 276 Query: 247 SIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFAS 306 ++++ G + ++GL ++IP+ +I+AM A R + ++ ++ G + Sbjct: 277 TLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGA 336 Query: 307 TF--GNSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAP 364 N+ + S+ AA G + LFW LP A+L AA IA INS+GNL GFV+P Sbjct: 337 ALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSP 396 Query: 365 TAFGFLEQRTGSIQGGLMGLAITSLVAAGVVFLARSGPGATRR 407 G+L+ T + G+ LA+ LV + LA + PG + Sbjct: 397 YLIGYLKDLTHNNATGMYMLAVM-LVVGSIATLA-TKPGMVNK 437 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 285 bits (729), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 158/392 (40%), Positives = 231/392 (58%), Gaps = 10/392 (2%) Query: 10 IDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMA 69 +DRVN+GF + + D+ YGLGAG+FF+GY +FEVPSN++L + GA+ W+ RIM Sbjct: 41 LDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMI 100 Query: 70 TWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGS 129 TWG+ + AMA+V + FY++RFLLG AEAGFFPGVI Y T W PA+ RG+ A+F++G Sbjct: 101 TWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGI 160 Query: 130 ALASILSGPISGGLLQIDGG--GLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLT 187 AL+ ++ GP+SG ++ G G GWQWMFI+E + S++L V ++ DA WL+ Sbjct: 161 ALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLS 220 Query: 188 AQERSAITSAIVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPS 247 +E+ + + I E++ Q +S + + ++ + IYF + +YG +FWLP+ Sbjct: 221 EEEKRLLEANISHEES-----QKEHLSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPT 275 Query: 248 IIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFAST 307 II+ G VGL +IP+ + AM A + ++ A I G+ ST Sbjct: 276 IIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRERRWHVAIPAFIGCIGLLLST 335 Query: 308 -FG-NSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPT 365 +G N+ A S+ A+VG LFW LP A+L AA IALINS+GNL GFV+P Sbjct: 336 QYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPF 395 Query: 366 AFGFLEQRTGSIQGGLMGLAITSLVAAGVVFL 397 G+L+ T S G+ LA SLV G++ L Sbjct: 396 LVGWLKDATQSTNAGMYVLA-ASLVIGGLLTL 426 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 284 bits (727), Expect = 9e-78, Method: Compositional matrix adjust. Identities = 168/390 (43%), Positives = 240/390 (61%), Gaps = 8/390 (2%) Query: 10 IDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMA 69 IDRVN+GF + + D+ A YGLGAG+FF+GY +FEVPSNLLL++ GA+ L RI Sbjct: 45 IDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITI 104 Query: 70 TWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGS 129 WGLA+ AMA+V + T+FY+LRFLLG EAGFFPGV+ Y T W PA R R +F++ Sbjct: 105 LWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSF 164 Query: 130 ALASILSGPISGGLLQ-IDG-GGLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLT 187 A+A + GPI+G ++ ++G G L WQW+FI+EG+ S+L V WL PA+AKWL+ Sbjct: 165 AIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPERPANAKWLS 224 Query: 188 AQERSAITSAIVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPS 247 E++A+T + E + KH S + + ++ I +YF + FW PS Sbjct: 225 EAEKNAVTQVLAAENSAPGKH----FSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPS 280 Query: 248 IIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFAS- 306 II+++G + Q+GL ++IP++ +AM + + ++ +A A +IA G+ A+ Sbjct: 281 IIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATG 340 Query: 307 -TFGNSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPT 365 T GN+ A S+ AA+G AA +FW +P A+L AAG IALINSIGNL GFVAP Sbjct: 341 MTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPY 400 Query: 366 AFGFLEQRTGSIQGGLMGLAITSLVAAGVV 395 G L+ TGS+ GL +A LVA+ +V Sbjct: 401 MIGALKTSTGSLSSGLYFVAALELVASFLV 430 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,222,027 Number of extensions: 48753 Number of successful extensions: 336 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 294 Number of HSP's successfully gapped: 20 Length of query: 409 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 322 Effective length of database: 1,207,925 Effective search space: 388951850 Effective search space used: 388951850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)