BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0400.p (411 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 798 0.0 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 562 e-161 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 290 1e-79 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 288 4e-79 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 286 3e-78 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 798 bits (2060), Expect = 0.0, Method: Compositional matrix adjust. Identities = 409/442 (92%), Positives = 409/442 (92%), Gaps = 31/442 (7%) Query: 1 VETPQPAASA-------------------------------YIDRVNIGFVRSHLATDVG 29 VETPQPAASA YIDRVNIGFVRSHLATDVG Sbjct: 8 VETPQPAASATGAAAPTVLQQAVAKIKQRVLPLFVIMFIVNYIDRVNIGFVRSHLATDVG 67 Query: 30 IGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTF 89 IGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTF Sbjct: 68 IGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTF 127 Query: 90 YVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQID 149 YVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQID Sbjct: 128 YVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQID 187 Query: 150 GGGLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQERSAITSAIVDEQAERMK 209 GGGL GWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQERSAIT AIVDEQAERMK Sbjct: 188 GGGLQGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQERSAITRAIVDEQAERMK 247 Query: 210 HQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIP 269 HQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIP Sbjct: 248 HQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIP 307 Query: 270 WLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFGNSVYAFVSICFAAVGFKA 329 WLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFGNSVYAFVSICFAAVGFKA Sbjct: 308 WLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFGNSVYAFVSICFAAVGFKA 367 Query: 330 ASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFGFLEQRTGSIQGGLMGLAIT 389 ASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFGFLEQRTGSIQGGLMGLAIT Sbjct: 368 ASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFGFLEQRTGSIQGGLMGLAIT 427 Query: 390 SLVAAGVVFLARSGPGATRRPR 411 SLVAAGVVFLARSGPGATRRPR Sbjct: 428 SLVAAGVVFLARSGPGATRRPR 449 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 562 bits (1449), Expect = e-161, Method: Compositional matrix adjust. Identities = 285/396 (71%), Positives = 332/396 (83%) Query: 11 YIDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIM 70 YIDRVNIGFVRSHL+TD+GIG AA+GLGAGLFFVGYALFEVPSN+LLQR+GA+AWLTRIM Sbjct: 37 YIDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYALFEVPSNMLLQRYGARAWLTRIM 96 Query: 71 ATWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSG 130 TWGL A MAFV SET+FY +RFLLG AEAGFFPGV+YYFTQWLP ERGRAMA+FLSG Sbjct: 97 FTWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSG 156 Query: 131 SALASILSGPISGGLLQIDGGGLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTA 190 SA+ASILSGPI+G LL I+GGGLHGWQWMFIIEG AS++ C VW LDS P +AKWL+ Sbjct: 157 SAIASILSGPITGALLLIEGGGLHGWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQ 216 Query: 191 QERSAITSAIVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSI 250 ++R A+ + I EQ +R ++PA VS LLRDPQI++FCFIYF+I LTIYGATFWLP+I Sbjct: 217 EQREALAAVIAAEQEQRAANRPAHVSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTI 276 Query: 251 IRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTF 310 I+KMG F+DFQVGLFNSIPW+++I AMY FA LAAR+K Q W AT LVI+AAGM S Sbjct: 277 IKKMGHFNDFQVGLFNSIPWIVAIAAMYLFAVLAARYKKPQFWVATTLVISAAGMAISAV 336 Query: 311 GNSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFG 370 V FV++CFA +GFKAASSLFWP+PQAYLDARIAA V+ALINS+GNLGGF AP FG Sbjct: 337 AGPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFG 396 Query: 371 FLEQRTGSIQGGLMGLAITSLVAAGVVFLARSGPGA 406 +LEQ+TGSIQGGL L+ SL+AA +VFLAR+ P A Sbjct: 397 YLEQKTGSIQGGLFALSGASLLAAVLVFLARTRPAA 432 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 290 bits (742), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 156/405 (38%), Positives = 239/405 (59%), Gaps = 12/405 (2%) Query: 10 AYIDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRI 69 AY+DRVN+GF + ++ D+G +GLGAGLFF+GY LFEVPSN+L+ + GA+ W+ RI Sbjct: 40 AYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARI 99 Query: 70 MATWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLS 129 M TWG+ + A FV + T FY+LRFLLG AEAGF+PG+I Y T W P+ R + +A+F+S Sbjct: 100 MITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMS 159 Query: 130 GSALASILSGPISGGLLQI--DGGGLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKW 187 G +A IL P+SG ++ GGL GWQWMF+IE + +VL+ +LD+ AKW Sbjct: 160 GIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKW 219 Query: 188 LTAQERSAITSAIV-DEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFW 246 L +E++++ + I D + + KH + ++D ++ + C IYFS + YG T W Sbjct: 220 LNDEEKASLQADIDGDAKGKESKHGFGAI-----VKDARVWLMCLIYFSFVMGQYGLTLW 274 Query: 247 LPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMF 306 +P++++ G + ++GL ++IP+ +I+AM A R + ++ ++ G Sbjct: 275 MPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRERRWHLVIPALMGGVGFV 334 Query: 307 ASTF--GNSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFV 364 + N+ + S+ AA G + LFW LP A+L AA IA INS+GNL GFV Sbjct: 335 GAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFV 394 Query: 365 APTAFGFLEQRTGSIQGGLMGLAITSLVAAGVVFLARSGPGATRR 409 +P G+L+ T + G+ LA+ LV + LA + PG + Sbjct: 395 SPYLIGYLKDLTHNNATGMYMLAVM-LVVGSIATLA-TKPGMVNK 437 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 288 bits (738), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 159/395 (40%), Positives = 234/395 (59%), Gaps = 10/395 (2%) Query: 9 SAYIDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTR 68 ++Y+DRVN+GF + + D+ YGLGAG+FF+GY +FEVPSN++L + GA+ W+ R Sbjct: 38 ASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIAR 97 Query: 69 IMATWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFL 128 IM TWG+ + AMA+V + FY++RFLLG AEAGFFPGVI Y T W PA+ RG+ A+F+ Sbjct: 98 IMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFM 157 Query: 129 SGSALASILSGPISGGLLQIDGG--GLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAK 186 +G AL+ ++ GP+SG ++ G G GWQWMFI+E + S++L V ++ DA Sbjct: 158 TGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDAD 217 Query: 187 WLTAQERSAITSAIVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFW 246 WL+ +E+ + + I E++ Q +S + + ++ + IYF + +YG +FW Sbjct: 218 WLSEEEKRLLEANISHEES-----QKEHLSLGAMFANGRVWLGALIYFCFVMGLYGVSFW 272 Query: 247 LPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMF 306 LP+II+ G VGL +IP+ + AM A + ++ A I G+ Sbjct: 273 LPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRERRWHVAIPAFIGCIGLL 332 Query: 307 AST-FG-NSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFV 364 ST +G N+ A S+ A+VG LFW LP A+L AA IALINS+GNL GFV Sbjct: 333 LSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAAAGIALINSLGNLAGFV 392 Query: 365 APTAFGFLEQRTGSIQGGLMGLAITSLVAAGVVFL 399 +P G+L+ T S G+ LA SLV G++ L Sbjct: 393 SPFLVGWLKDATQSTNAGMYVLA-ASLVIGGLLTL 426 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 286 bits (731), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 169/392 (43%), Positives = 242/392 (61%), Gaps = 8/392 (2%) Query: 10 AYIDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRI 69 A+IDRVN+GF + + D+ A YGLGAG+FF+GY +FEVPSNLLL++ GA+ L RI Sbjct: 43 AWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARI 102 Query: 70 MATWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLS 129 WGLA+ AMA+V + T+FY+LRFLLG EAGFFPGV+ Y T W PA R R +F++ Sbjct: 103 TILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMT 162 Query: 130 GSALASILSGPISGGLLQ-IDG-GGLHGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKW 187 A+A + GPI+G ++ ++G G L WQW+FI+EG+ S+L V WL PA+AKW Sbjct: 163 SFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPERPANAKW 222 Query: 188 LTAQERSAITSAIVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWL 247 L+ E++A+T + E + KH S + + ++ I +YF + FW Sbjct: 223 LSEAEKNAVTQVLAAENSAPGKH----FSFKDACTNYRVWICAAVYFCVVSGNATIAFWA 278 Query: 248 PSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFA 307 PSII+++G + Q+GL ++IP++ +AM + + ++ +A A +IA G+ A Sbjct: 279 PSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVA 338 Query: 308 S--TFGNSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVA 365 + T GN+ A S+ AA+G AA +FW +P A+L AAG IALINSIGNL GFVA Sbjct: 339 TGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVA 398 Query: 366 PTAFGFLEQRTGSIQGGLMGLAITSLVAAGVV 397 P G L+ TGS+ GL +A LVA+ +V Sbjct: 399 PYMIGALKTSTGSLSSGLYFVAALELVASFLV 430 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,243,442 Number of extensions: 49694 Number of successful extensions: 335 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 293 Number of HSP's successfully gapped: 20 Length of query: 411 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 323 Effective length of database: 1,203,190 Effective search space: 388630370 Effective search space used: 388630370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)