BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0396.p (318 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [... 625 e-180 gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [... 120 2e-28 gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herba... 102 4e-23 >gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 625 bits (1613), Expect = e-180, Method: Compositional matrix adjust. Identities = 314/318 (98%), Positives = 317/318 (99%) Query: 1 MKIAIVGAGAMGGLFAARLALAGQDVSLIEVAPASIEAISSHGLSLTDEDGAHTVRVPVG 60 MKIAIVGAGAMGGLFAARLALAGQDVSLIEVAPASIEAISSHGLSLTDEDGAHTVRVPVG Sbjct: 1 MKIAIVGAGAMGGLFAARLALAGQDVSLIEVAPASIEAISSHGLSLTDEDGAHTVRVPVG 60 Query: 61 RADSFRDTFDLIILFTKGFHTQAAIASVAHLAGPHTWALSVQNGLGNADLIAEVIPRERI 120 RADSF+D FDLIILFTKGFHTQAAIASVAHLAGPHTWALSVQNGLGNADLIAEVIPRERI Sbjct: 61 RADSFQDAFDLIILFTKGFHTQAAIASVAHLAGPHTWALSVQNGLGNADLIAEVIPRERI 120 Query: 121 IVGMTNLPADLRAPGVVHSHGEGQVRVWTLSGQPSAQLTQVAADFNAAGLPCSADPLVQQ 180 IVGMTNLPADLRAPGVVHSHGEGQVR+WTLSGQPSAQLTQVAADFNAAGLPCSADPLVQQ Sbjct: 121 IVGMTNLPADLRAPGVVHSHGEGQVRIWTLSGQPSAQLTQVAADFNAAGLPCSADPLVQQ 180 Query: 181 AIWEKLAFNAALNAICAVTGLTVGAAGASASGRELALRIAAETVAVAQAAGIEARLVQVE 240 AIWEKLAFNAALNAICAVTGLTVGAAGASASGRELALRIAAETVAVAQAAGI+ARLVQVE Sbjct: 181 AIWEKLAFNAALNAICAVTGLTVGAAGASASGRELALRIAAETVAVAQAAGIDARLVQVE 240 Query: 241 DAIEHAFRTHLHHKPSMLQDIEAGRRTEIDFINGAVVREAERLGLPVPTTQTLHQLVKLM 300 DAIEHAFRTHLHHKPSMLQDIEAGRRTEIDFINGAVVREAERLGLPVPTTQTLHQLVKLM Sbjct: 241 DAIEHAFRTHLHHKPSMLQDIEAGRRTEIDFINGAVVREAERLGLPVPTTQTLHQLVKLM 300 Query: 301 EGAAQAQQRPAAQTSESS 318 EGAAQAQQRPAAQTSESS Sbjct: 301 EGAAQAQQRPAAQTSESS 318 >gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 97/309 (31%), Positives = 149/309 (48%), Gaps = 16/309 (5%) Query: 1 MKIAIVGAGAMGGLFAARLALAGQDVSLIEVAPASIEAISSHGLSLTDEDGAHTVRVPVG 60 MK+A++GAGA+G FA LA G V+L+ P +EA ++ GL L + T+ V Sbjct: 1 MKVAVMGAGAVGCYFAGMLARVGHQVALVG-RPQHVEAFNTRGLRLETKAFDETIAVKAS 59 Query: 61 RADSFRDTFDLIILFTKGFHTQAAIASVAHLAGPHTWALSVQNGLGNADLIAEVIPRERI 120 + +L++ K T+ A A +A P T LS+QNG NA+ + + + + Sbjct: 60 TDAAIAADAELVLFCVKSPDTETAGAQLAPHLAPDTLVLSLQNGTDNAERL-RTVMAQPV 118 Query: 121 IVGMTNLPADLRAPGVVHSHGEGQVRVWTLSGQPSAQLTQVAADFNAAGLPCSADPLVQQ 180 + + ++ PG V HG G++ + +P+A A AAG+P V+ Sbjct: 119 AAAVVYVATEMAGPGHVKHHGRGELVI-----EPAAGSRDAADALVAAGVPTEISDNVRG 173 Query: 181 AIWEKLAFNAALNAICAVTGLTVGAAGASASGRELALRIAAETVAVAQAAGIEARLVQVE 240 +W KL N A NA+ A+ L G E+ ++ E AVA A G V + Sbjct: 174 ELWLKLILNCAYNALSAIAQLPYGKLVEGPGIPEVIAQLVTECKAVAAAEG-----VTLA 228 Query: 241 DAIEHAFRTHLHHKP----SMLQDIEAGRRTEIDFINGAVVREAERLGLPVPTTQTLHQL 296 D ++ A R + P S QD+ G+ +EID +NG +V A+ GL P T+H L Sbjct: 229 DDVDSAVRKIVQTMPGQFSSTAQDLARGKPSEIDHLNGFIVARAQAHGLSAPANLTVHAL 288 Query: 297 VKLMEGAAQ 305 VKL+E AQ Sbjct: 289 VKLIESKAQ 297 >gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 102 bits (255), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 97/333 (29%), Positives = 152/333 (45%), Gaps = 34/333 (10%) Query: 1 MKIAIVGAGAMGGLFAARLALAGQDVSLIEVAPASIEAISSHGLSLTDEDGAHTVRVPVG 60 ++I I GAGA+GG A RLA AG VS++ +++AI HGL+L D G R Sbjct: 15 LRICIAGAGAIGGTLAVRLAAAGHQVSVLARG-QTLQAIREHGLTLHDLHGTAHARPAAD 73 Query: 61 RADSFRDTFDLIILFTKGFHTQAAIASVAHLAGPHT-----------WALSVQNGLGNAD 109 SF D+I L K + VA + G T W + G + Sbjct: 74 HRPSF-GVQDVIFLCAKSQDMAGLLPQVAPMVGETTVVIPTNNGVPWWYFHREGGRFDGQ 132 Query: 110 LIAEVIPR---------ERIIVGMTNLPADLRAPGVVHSHGEGQVRVWTLSGQPSAQLTQ 160 + V P +II + + A++ + GV+ S + + SG+ S +L + Sbjct: 133 TVKAVDPEGALAAALPLAQIIGSVLFITAEVESAGVIRSVNPHLMVLGEPSGEMSERLLR 192 Query: 161 VAADFNAAGLPCSADPLVQQAIWEKLAFNAALNAICAVTGLTVGAAGASASGRELALRIA 220 V A +AG+ A ++ +W K+ N + N + +T T+ RE+ +I Sbjct: 193 VRAAVESAGIEARATDRIRDKLWTKIIANISTNPLSVITQGTLQQLYGLPELREVVSQIM 252 Query: 221 AETVAVAQAAGIEARL-----VQVEDAIEHAFRTHLHHKPSMLQDIEAGRRTEIDFINGA 275 ET+ VA + G + +Q+ +A+ AFRT SMLQD+E GR E+ I A Sbjct: 253 RETLLVASSHGARVDIDPLTFLQLGEAM-GAFRT------SMLQDLERGRPLELAAIGDA 305 Query: 276 VVREAERLGLPVPTTQTLHQLVKLMEGAAQAQQ 308 V+ AER +P+P T+ + L + AAQ Q+ Sbjct: 306 VLEMAERYAIPMPITRAIVSLARFRGEAAQQQR 338 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 827,244 Number of extensions: 33104 Number of successful extensions: 138 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 3 Length of query: 318 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 233 Effective length of database: 1,217,395 Effective search space: 283653035 Effective search space used: 283653035 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)