BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0395.p (460 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 897 0.0 gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 191 8e-50 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 164 1e-41 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 134 1e-32 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 103 3e-23 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 897 bits (2317), Expect = 0.0, Method: Compositional matrix adjust. Identities = 455/460 (98%), Positives = 455/460 (98%) Query: 1 MSNLGAHGNQTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWG 60 MS LGAHGNQTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWG Sbjct: 1 MSKLGAHGNQTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWG 60 Query: 61 ITRNAFAPAVAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTI 120 ITRNAFAPAVAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTI Sbjct: 61 ITRNAFAPAVAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTI 120 Query: 121 AILRFCAGLGIGGALPSSTTMTAEFSPARRRTLTVTATIVCVPLGGMLAGLFANAVLPHY 180 AILRFCAGLGIGGALPSSTTMTAEFSPARRRTL VTATIVCVPLGGMLAGLFANAVLPHY Sbjct: 121 AILRFCAGLGIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHY 180 Query: 181 GWRGLFLIGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFAD 240 GWRGLFLIGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFAD Sbjct: 181 GWRGLFLIGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFAD 240 Query: 241 AREQAIETRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAG 300 AREQAIETRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAG Sbjct: 241 AREQAIETRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAG 300 Query: 301 AGLTAYNFGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGI 360 AGLTAYNFGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGI Sbjct: 301 AGLTAYNFGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGI 360 Query: 361 GLHGLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVITQGGAAG 420 GLHGLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVITQGGAAG Sbjct: 361 GLHGLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVITQGGAAG 420 Query: 421 YLAMLGGAMLMVFVALLVVRKHIPRVNAGAAEILKKEPHA 460 YLAML GAMLMVFVALLVVRKHIPRVNAGAAE LKKE HA Sbjct: 421 YLAMLAGAMLMVFVALLVVRKHIPRVNAGAAETLKKESHA 460 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 191 bits (486), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 134/410 (32%), Positives = 213/410 (51%), Gaps = 16/410 (3%) Query: 11 TVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAPAV 70 TV + ++++ G F FQ ++ L L +++DGFD Q +G+ P++I +WG+++ + P Sbjct: 11 TVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGPVF 70 Query: 71 AAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIA---ILRFCA 127 AGL GM +GS DR+GRR +I S F A ++A +P VTT+ +LRF Sbjct: 71 GAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFF--ALCMLA-TPMVTTLDQLLVLRFIT 127 Query: 128 GLGIGGALPSSTTMTAEFSPARRRTLTVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFL 187 G G+G +P++ + EFSP+ R + +G G + A++P YGW +F Sbjct: 128 GFGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFW 187 Query: 188 IGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFADAREQAI- 246 +GG +P+ + + +PES ++L H ++ R L+++ + D D E + Sbjct: 188 VGGAVPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVID----DKTEVVVK 243 Query: 247 ETRAG---FGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGL 303 ET+A ALF G+A T+ LW+ FMNL+ +Y +WLPT++ G S S+A Sbjct: 244 ETKAEGMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIG 303 Query: 304 TAYNFGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGIGLH 363 T+ GGV G L FI RFG + C L A S + L A+ LL V + + Sbjct: 304 TSLQVGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKL--ATVPALLFVAVIVA 361 Query: 364 GLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVI 413 G + Q + AL YPT +R+TG AL GR+G+++ G +I Sbjct: 362 GFCIVGGQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLI 411 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 164 bits (415), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 131/402 (32%), Positives = 201/402 (50%), Gaps = 9/402 (2%) Query: 10 QTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAPA 69 +T+D+ +++D F+ F +V+CL AL +V DG+D + G +P L+KEW ++ Sbjct: 2 KTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGAL 61 Query: 70 VAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCAGL 129 + L GM G+ G ADR GR+ + S +F V+T L F+ T + RF AGL Sbjct: 62 GSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGL 121 Query: 130 GIGGALPSSTTMTAEFSPARRRTLTVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFLIG 189 G GG +P++ + E++P R R+ V LGG+L +LP++GW+ +F Sbjct: 122 GCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFAA 181 Query: 190 GLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRP--VSADTGFADAREQAIE 247 + V V+LW +PES +L R R ++ R +L ++ ++ DT A + Sbjct: 182 AVPLVLLPVILW-KLPESVGFLLRQ-GRLEQARAMLTQVDPQARIATDTQLVQADVKG-- 237 Query: 248 TRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLTAYN 307 A LF +A T+ +WV+FF LL VY+ SWLP ++A G S + + L A N Sbjct: 238 AGAPMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALN 297 Query: 308 FGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGIGLHGLFV 367 FGGV GA+ RF LP ++ T + + L S T +L + I + G Sbjct: 298 FGGVVGAIGGGWLGDRF--TLPKVVVSF-FTVGTVAIALLGFNSPTPVLYLLIIVAGATT 354 Query: 368 NAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAG 409 Q +YA A YP +R+TG A GR GAI+ G Sbjct: 355 IGTQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLG 396 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 134 bits (338), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 131/426 (30%), Positives = 199/426 (46%), Gaps = 41/426 (9%) Query: 1 MSNLGA---HGNQTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIK 57 MS L A G Q +G+ FQ + + L + D IGF+ P++ K Sbjct: 1 MSTLPASVEQGLQAAGVGK---------FQYRLFVIFGLVWLADAMQVLSIGFSAPTIAK 51 Query: 58 EWGITRNAFAPAVAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFV---FGVATSLIAFS 114 +G+T VGM +G+ G ADR GRR ++G+V + FGVA+ AF+ Sbjct: 52 TFGLTVPQALQTGTMFFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVAS---AFA 108 Query: 115 PDVTTIAILRFCAGLGIGGALPSSTTMTAEFSPARRR--------TLTVTATIVCVPLGG 166 PD T + LR G+G+GG LP TM AEF P+ RR + TI C+ + Sbjct: 109 PDFTWLLALRLLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTI-CLAVLA 167 Query: 167 MLAGLFANAVLPHYGWRGLFLIGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQ 226 ++A + N WR +F + G LP V+L F +PESP YL R + D R++L+ Sbjct: 168 LVAVSWGND-----AWRVIFFVTG-LPALVGVVLRFYVPESPMYLNRS-GKSDAARQVLE 220 Query: 227 RMSRPVSADTGFADAREQAIETRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLP 286 R+++ + T + + +E R ALF++ R +++L++A+ + +A Y F +LP Sbjct: 221 RVAKVNGSSTPIPPLQPEVVE-RKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLP 279 Query: 287 TMLAGEGLSPSVAGAGLTAYNFGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRS 346 L EG L + G L A + R+G R P L+ L +A L S Sbjct: 280 VKLGSEGFGFMRGQIFLIFLALVQLPGYALSAYGVERWG-RKPTLVGFLVLSAVGCMLYS 338 Query: 347 LDVASQTTLLVVGIGLHGLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSA 406 L L+V L + + +YA +YPT +RA+G A A R G + Sbjct: 339 L--GKDPMLVVGSTLLLSFSLLGTWAALYAFTPEVYPTDLRASGMGTAGAVARFGGL--- 393 Query: 407 FAGALV 412 FA A+V Sbjct: 394 FAPAIV 399 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 103 bits (257), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 113/453 (24%), Positives = 193/453 (42%), Gaps = 26/453 (5%) Query: 18 LDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAPAVAAGLVGM 77 +++ FS + + + + D F + F +P L+ W ++ +A+G +G Sbjct: 33 IENVPFSRWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQ 92 Query: 78 GIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCAGLGIGGALPS 137 IG+ F G A+R+GR + +V + V + + A + + + RF G+G+GG +P Sbjct: 93 VIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPV 152 Query: 138 STTMTAEFSPARRRTLTVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFLIGGLLPVAFS 197 + T E S A R + PLG + A ++P +GW +FL+GG +P Sbjct: 153 AATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPRFGWEYMFLVGG-IPGILV 211 Query: 198 VLLWFVIPESPRYL-ARHPHRWDELRRLLQRMSRPVSADTGFADAREQAIETR-----AG 251 LL +PESPR+L AR R D+ +L++ + A +QAIE R AG Sbjct: 212 ALLITRLPESPRWLIAR--GRLDDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAG 269 Query: 252 FGA--------LFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGE---GLSPSVAG 300 G LF+ T+ +W+ + + +WLP++ L S+ Sbjct: 270 AGKRQRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRM 329 Query: 301 AGLTAYNFGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGI 360 A ++ N L CAL I R G R + + + A L L S +++ + Sbjct: 330 ASVS--NVLSACAVLGCALLIDRVGRRRWAIASFILSGALLALLGVLKAESAWSVMFLAS 387 Query: 361 GLHGLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVITQGGAAG 420 + + + +Y IYPT++RA GT A ++ R + + +V+ G A Sbjct: 388 SAYAV-MGTTTVLLYLYTPEIYPTRMRAIGTGLATSWLRAASAAAPAMVGVVLAGHGIAT 446 Query: 421 YLAMLGGAMLMVFVALLVVRKHIPRVNAGAAEI 453 M G ++ LL + I N EI Sbjct: 447 VFLMFAGVTVL---GLLAAWRMIETTNRSLEEI 476 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,419,029 Number of extensions: 63467 Number of successful extensions: 512 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 478 Number of HSP's successfully gapped: 30 Length of query: 460 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 372 Effective length of database: 1,203,190 Effective search space: 447586680 Effective search space used: 447586680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)