BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0394.p (483 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 964 0.0 gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 279 4e-76 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 268 6e-73 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 255 6e-69 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 240 2e-64 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 964 bits (2492), Expect = 0.0, Method: Compositional matrix adjust. Identities = 482/483 (99%), Positives = 483/483 (100%) Query: 1 MNDIDMLINGERRGATGAAYFERRNPLDHSVASRAPAATVADARAAVAAAAAAFPAWAAI 60 MNDIDMLINGERRGATGAAYFERRNPLDHSVA+RAPAATVADARAAVAAAAAAFPAWAAI Sbjct: 1 MNDIDMLINGERRGATGAAYFERRNPLDHSVAARAPAATVADARAAVAAAAAAFPAWAAI 60 Query: 61 GPGQRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIA 120 GPGQRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIA Sbjct: 61 GPGQRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIA 120 Query: 121 GEVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPA 180 GEVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPA Sbjct: 121 GEVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPA 180 Query: 181 THGLIIAALQEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQ 240 THGLIIAALQEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQ Sbjct: 181 THGLIIAALQEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQ 240 Query: 241 CARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADE 300 CARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADE Sbjct: 241 CARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADE 300 Query: 301 FVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTL 360 FVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTL Sbjct: 301 FVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTL 360 Query: 361 MPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARAL 420 MPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARAL Sbjct: 361 MPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARAL 420 Query: 421 ALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFGHFGGKAGIAEFTELRWVTVQTTPRH 480 ALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFGHFGGKAGIAEFTELRWVTVQTTPRH Sbjct: 421 ALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFGHFGGKAGIAEFTELRWVTVQTTPRH 480 Query: 481 YPF 483 YPF Sbjct: 481 YPF 483 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 279 bits (713), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 166/424 (39%), Positives = 238/424 (56%), Gaps = 7/424 (1%) Query: 62 PGQRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAG 121 P +R + AA D+ A+ +A ETG V+ A AA M Q G Sbjct: 69 PRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQG 128 Query: 122 EVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPAT 181 +++PS PG L++ R P GV+ I+P+N P+IL +R +A LA GN VV K P + Sbjct: 129 QLLPS-TPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVS 187 Query: 182 HGLIIAAL-QEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQ 240 G ++A + ++AGLP G+++ + E EA++ P V + FTGS VGR I Sbjct: 188 GGFMLARIFEQAGLPAGLLHVLPGGAETG----EALVTAPGVPMIAFTGSPAVGRRIGEL 243 Query: 241 CARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADE 300 RHLK LELGG ++L DADLD A AA+GA+ + GQICM+ RI+V S+ + Sbjct: 244 AGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEG 303 Query: 301 FVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTL 360 ++RLA KA LP+GD G V LG ++D ++R +AL+ +++A GA LV GGR Sbjct: 304 LLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQLF 363 Query: 361 MPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARAL 420 T+L V M ++ EE FGPV ++R DE AIA AN + GL++ + S V RA+ Sbjct: 364 YQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRAM 423 Query: 421 ALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFG-HFGGKAGIAEFTELRWVTVQTTPR 479 A++ R+ G+ H+N TV+DE PFGG +G G GG A E+T+ RW T++ Sbjct: 424 AVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQQAM 483 Query: 480 HYPF 483 YPF Sbjct: 484 PYPF 487 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 268 bits (686), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 166/472 (35%), Positives = 243/472 (51%), Gaps = 9/472 (1%) Query: 7 LINGERRGATGAAYFERRNPLDHSVASRAPAATVADARAAVAAAAAAFPAWAAIGPGQRR 66 +NG+ A NP + + P A+ R A+ AA AA+PAW +R Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 67 ALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAGEVIPS 126 A+L + + A DD A M AE G A + AA + A + G+ IPS Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPS 134 Query: 127 DVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPATHGLII 186 P N + +++ GV I PWN P + R LA G +V K +E P + L + Sbjct: 135 PSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFS-ALAL 193 Query: 187 AALQE-AGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQCARHL 245 A L E AG+P GV + VT P+ I + + P VR+++FTGST VG+++ Q A + Sbjct: 194 AVLAERAGIPAGVFSVVTGTPK---GIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSI 250 Query: 246 KPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADEFVRRL 305 K LELGG APFIV DADLDAAVE A + N+GQ C+ RI V D V D F +L Sbjct: 251 KKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKL 310 Query: 306 AAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGR---ASDTLMP 362 +G + V G +++ V++ + DA+AKGA ++ GG+ + Sbjct: 311 VEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFE 370 Query: 363 ATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARALAL 422 T+L V+ M + REE+FGP+ P+ R DE +A AND E+GL+S +SRD+ R + Sbjct: 371 PTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRV 430 Query: 423 SRRIESGICHINGPTVHDEAQMPFGGVKSSGFGHFGGKAGIAEFTELRWVTV 474 + +ESG+ IN + +E PFGGVK SG G G GI ++ ++++ + Sbjct: 431 AEGLESGMVGINTGLISNEV-APFGGVKQSGLGREGSHYGIDDYLVVKYLCM 481 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 255 bits (651), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 157/475 (33%), Positives = 241/475 (50%), Gaps = 7/475 (1%) Query: 2 NDIDMLINGERRGATGAAYFERRNPLDHSVASRAPAATVADARAAVAAAAAAFPAWAAIG 61 D+ + INGE + + NP V + A AD A+ AA+ F W + Sbjct: 3 KDVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMS 62 Query: 62 PGQRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAG 121 +R ++ AAD L+ RA++ A+ M E G A AAD + A + G Sbjct: 63 AFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYG 122 Query: 122 EVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPAT 181 ++P+ VPG M +++P G V PWN P+ VR ++ LA G +++ K E PA+ Sbjct: 123 RLVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPAS 182 Query: 182 HGLIIAALQEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQC 241 +I A +AG+P GV+N V P A I E +I P +++++FTGST VG+ +AA Sbjct: 183 PAELIRAYADAGVPAGVINLVYGVP---AEISEYLIPHPVIKKISFTGSTPVGKQLAALA 239 Query: 242 ARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADEF 301 +H+K A +ELGG AP +V DAD+D A + A F N+GQ+C+S R +V V + F Sbjct: 240 GQHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAF 299 Query: 302 VRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGR---ASD 358 V + A L +G+ + + +G V + + ALIDDA+++GA L GG+ Sbjct: 300 VDKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKG 359 Query: 359 TLMPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVAR 418 T+L ++ EE FGP+ I + I AN YGL++ F++ Sbjct: 360 YFFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHT 419 Query: 419 ALALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFGHFGGKAGIAEFTELRWVT 473 AL+ R+ESG+ IN + ++PFGG+K SG+G GG I + + VT Sbjct: 420 KHALAARVESGMLTINHLGLA-LPELPFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 240 bits (612), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 178/474 (37%), Positives = 253/474 (53%), Gaps = 8/474 (1%) Query: 6 MLINGERRGATGAAYFERRNPLDHSVASRAPAATVADARAAVAAAAAAFPAWAAIGPGQR 65 LI + RGA + +P V + P ADARAAV AA AAF AW A QR Sbjct: 16 QLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAWRATPAKQR 75 Query: 66 RALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAGEVIP 125 ++ D L A DD + ++ E G A V AA + T+ G++IP Sbjct: 76 AGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIP 135 Query: 126 SDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPATHGLI 185 + V G M +++P GVV I PWN P + R IA LA G TVV K +E P T + Sbjct: 136 APVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLAL 195 Query: 186 IAALQEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQCARHL 245 + QEAG+P GV+N VT + E +V+ +A+ VR+++FTGST VG+ +A A L Sbjct: 196 VRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSADTL 255 Query: 246 KPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADEFVRRL 305 K LELGG APFIV DAD+DAA++ F N GQ C+S RI V + V D FV +L Sbjct: 256 KKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVDKL 315 Query: 306 AAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGR-------ASD 358 A+ A+L +G +G +++ + + + + DA+A+GA ++ GG+ SD Sbjct: 316 GARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQGPGFGSD 375 Query: 359 TLMPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVAR 418 T+L V+ M EE+FGPV PI R A ++ IA AN +GL++ +S DV R Sbjct: 376 NYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFYSTDVRR 435 Query: 419 ALALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFGHFGGKAGIAEFTELRWV 472 ++ +ESGI +N + EA PFGGVK SG+G G G+ ++ ++V Sbjct: 436 IHRVTDALESGIVGVNEGALAAEA-APFGGVKESGYGREGSVHGLDDYLHTKYV 488 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,395,132 Number of extensions: 55313 Number of successful extensions: 246 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 20 Length of query: 483 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 394 Effective length of database: 1,198,455 Effective search space: 472191270 Effective search space used: 472191270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)