BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0393.p (532 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 1062 0.0 gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 97 3e-21 gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspi... 74 2e-14 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 68 2e-12 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 1062 bits (2747), Expect = 0.0, Method: Compositional matrix adjust. Identities = 530/532 (99%), Positives = 530/532 (99%) Query: 1 MQQKFPHPITVRHAVLDLMRRLGMTSVFANPGSTELPMFRDFPEDFRYVLGLQEAVVVGM 60 MQQKFPHPITVRHAVLDLMRRLGMTSVFANPGSTELPMFRDFPEDFRYVLGLQEAVVVGM Sbjct: 1 MQQKFPHPITVRHAVLDLMRRLGMTSVFANPGSTELPMFRDFPEDFRYVLGLQEAVVVGM 60 Query: 61 ADGFAQASGNAALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGAT 120 ADGFAQASGNAALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGAT Sbjct: 61 ADGFAQASGNAALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGAT 120 Query: 121 QATELAKPYLKWSIEPARAADVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPT 180 QATELAKPYLKWSIEPARAADVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPT Sbjct: 121 QATELAKPYLKWSIEPARAADVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPT 180 Query: 181 QVTSRCRPEPAAIATLAAALEQSQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMS 240 QVTSRCRPEPAAIA LAAALEQSQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMS Sbjct: 181 QVTSRCRPEPAAIARLAAALEQSQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMS 240 Query: 241 SRCSFPEDHPLFAGFLPAMREKIVQALQGHDLLFALGAPAFTYHVEGSGPHVPPGAALFQ 300 SRCSFPEDHPLFAGFLPAMREKIVQALQGHDLLFALGAPAFTYHVEGSGPHVPPGAALFQ Sbjct: 241 SRCSFPEDHPLFAGFLPAMREKIVQALQGHDLLFALGAPAFTYHVEGSGPHVPPGAALFQ 300 Query: 301 LIDDPVTASWTPTGQAIVGNIQLGLDDLLAQIRRQPPTQRVQPPARPARARVEASAPLSM 360 LIDDPVTASWTPTGQAIVGNIQLGLDDLLAQIRRQPPTQR QPPARPARARVEASAPLSM Sbjct: 301 LIDDPVTASWTPTGQAIVGNIQLGLDDLLAQIRRQPPTQRAQPPARPARARVEASAPLSM 360 Query: 361 AYALQTLADVREAGDIVVEEAPGSRPVMQAHLPFTRPGSFYTMASGGLGYGMPAAVGVAL 420 AYALQTLADVREAGDIVVEEAPGSRPVMQAHLPFTRPGSFYTMASGGLGYGMPAAVGVAL Sbjct: 361 AYALQTLADVREAGDIVVEEAPGSRPVMQAHLPFTRPGSFYTMASGGLGYGMPAAVGVAL 420 Query: 421 ARPHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAALQDFAPVFGFGPEDP 480 ARPHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAALQDFAPVFGFGPEDP Sbjct: 421 ARPHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAALQDFAPVFGFGPEDP 480 Query: 481 VHGTELPGLDFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLVEIVVR 532 VHGTELPGLDFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLVEIVVR Sbjct: 481 VHGTELPGLDFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLVEIVVR 532 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 97.1 bits (240), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 133/535 (24%), Positives = 222/535 (41%), Gaps = 44/535 (8%) Query: 23 GMTSVFANPGSTELPMFRD-FPED-FRYVLGLQEAVVVGMADGFAQASGNAALVNLHSAA 80 G+ VF PG L ++ F +D F+++L E V AD ++++S + + S Sbjct: 20 GVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSQKVGVAIVTSGP 79 Query: 81 GVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGATQATELAKPYLKWSIEPARAA 140 GV NA+ + TA+ + P+++ +GQ + + D F + +P +K + Sbjct: 80 GVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAF-QECDTVGITRPCVKHNFLVKDVK 138 Query: 141 DVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPTQVTSRC-----RPEPAAIAT 195 D+ + +A+++A GPV V IP D P V R + I Sbjct: 139 DLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVEMRSYKPVDKGHSGQIRK 198 Query: 196 LAAALEQSQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMSSRCSFPEDHPLFAGF 255 L ++RP I G + A A L + L + ++ F G Sbjct: 199 ALQLLLSAERPMIYTGGGIILANAAPELNKLVDRL-GYPCTNTLMGLGAYRSSSDKFVG- 256 Query: 256 LPAMR--EKIVQALQGHDLLFALGAPAFTYHVEGSGPHVPPGAALFQLID-DPVTASW-T 311 +P M + A+Q D+L A+GA F V G+ H A ID DP + S Sbjct: 257 MPGMHGTYEANMAMQHCDVLIAIGA-RFDDRVIGNPKHFASHARKIIHIDIDPSSISKRV 315 Query: 312 PTGQAIVGNIQLGLDDLLAQIR---------------RQPPTQRVQPPARPARARVEASA 356 IVGN++ L +LL+Q+ +Q R + + A + E Sbjct: 316 KVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRKRDCLKFATSE-EFIK 374 Query: 357 PLSMAYALQTLADVREAGDIVVEEAPGSRPVMQA-HLPFTRPGSFYTMASGGL---GYGM 412 P S+ +Q + +V + GD + G + A + F +P + + SGGL G G+ Sbjct: 375 PQSV---VQKVWEVTK-GDAFITSDVGQHQMWAAQYYGFDKPRRW--INSGGLGTMGVGL 428 Query: 413 PAAVGVALARPHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAALQDFAPV 472 P A+GV +A P + V + G+ S Q + + Q L ++LNN ++ + + Sbjct: 429 PYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMVRQWQQI 488 Query: 473 FGFGPEDPVHGTELPGLDFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLV 527 + LP DF LA S G G+++ + + ALREA + RLV Sbjct: 489 DYGSRYSESYMDSLP--DFNKLAESYGHVGMKIEKPGDVDGALREAFAMKD-RLV 540 >gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 74.3 bits (181), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 47/133 (35%), Positives = 65/133 (48%), Gaps = 5/133 (3%) Query: 13 HAVLDLMRRLGMTSVFANPGSTELPMFR---DFPEDFRYVLGLQEAVVVGMADGFAQASG 69 HA+ + G+ + FANPG+TEL + R FP+ R V+ LQE V G ADG+ + G Sbjct: 40 HALWAGLLDQGVDTCFANPGTTELDLVRGLESFPK-LRCVVALQENVCTGAADGYGRMLG 98 Query: 70 NAALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGATQATELAKPY 129 A LH G N + N+ A R RTP++ G LP+D L ++ LA P Sbjct: 99 RPASTLLHLGPGFANGIANLHNARRARTPIVNIIGDHTSWHLPYDAPL-SSDIESLAHPV 157 Query: 130 LKWSIEPARAADV 142 W AD+ Sbjct: 158 SGWVHRITGTADI 170 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 67.8 bits (164), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 96/400 (24%), Positives = 161/400 (40%), Gaps = 34/400 (8%) Query: 159 GPVFVSIPVD----DWDQPAELVPPTQVTSRCR-PEPAAIATLAAALEQSQRPAIVVGAE 213 GPV +++P D +D P + P + R + P + A L Q+++P ++ G Sbjct: 187 GPVTLALPQDVQTMAYDYPLDFFSPRVIVPRAQAPAAQELEDAVALLRQARQPLLIAGGG 246 Query: 214 LDRAGAWDAAIALAETLRARVFVAPMSSRCSFPEDHPLFAGFLPAMREKIVQAL-QGHDL 272 + A + AE V + + + P H L G + AL Q D+ Sbjct: 247 VLYGNACEHLRRFAEQHGVPV-AETQAGKSALPWSHALQMGAIGVTGSPAANALAQEADV 305 Query: 273 LFALGAPAFTYHVEGSGPHVPPGAALFQLIDDPVTASWTPTGQAIVGNIQLGLDDL---L 329 + A+G + +G H A ++ + G A+ + LGL+ L L Sbjct: 306 VIAVGTRLQDFT---TGSHTLFAQARLLNLNVNAMDAHKWRGLALQADAGLGLEALSHAL 362 Query: 330 AQIRRQPPTQ-RVQPPARPARARV-----EASAPLSMAYALQTLADVREA------GDIV 377 R P R A+ R RV + + Y + + ++ + DIV Sbjct: 363 EGWRSAPEWHARAHTLAQDWRERVAQITGQTDTGGRLPYDGEVIGAIQRSVADSPSQDIV 422 Query: 378 VEEAPGSRPV-MQAHLPFTRPGSFYT-MASGGLGYGMPAAVGVALARPHSRVIGLIGDGS 435 V A G+ P + RPG+++ +GY + +GV LA+P VI ++GDGS Sbjct: 423 VC-AAGTLPAELHKLWRAGRPGAYHVEYGYSCMGYEVAGGLGVKLAQPQREVIVIVGDGS 481 Query: 436 AMYSPQAIYSAVQLGLPITFVILNNGRYAALQDFAPVFGFGPEDPVHGTEL------PGL 489 + + ++V LG + V+L+N Y + G P + + L P + Sbjct: 482 YLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMLADCLQAGPGAPAI 541 Query: 490 DFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLVEI 529 DF A A S+G G V L AL+ A ++ + LV I Sbjct: 542 DFAAHARSLGALGENVKTITELEAALQRARAADRSYLVCI 581 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,723,026 Number of extensions: 78360 Number of successful extensions: 278 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 4 Length of query: 532 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 442 Effective length of database: 1,193,720 Effective search space: 527624240 Effective search space used: 527624240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)