BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0389.p (467 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 867 0.0 gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 96 5e-21 gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 90 5e-19 gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 86 5e-18 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 867 bits (2241), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/438 (99%), Positives = 437/438 (99%) Query: 30 MPPVEIRQPLVMPATAAARPSYLARIQRRYEQGNQPIGELQNGLFCSRKSDIYWTAKGSE 89 MPPVEIRQPLVMPATAAARPSYLARIQRRYEQGNQPIGELQNGLFCSRKSDIYWTAKGSE Sbjct: 1 MPPVEIRQPLVMPATAAARPSYLARIQRRYEQGNQPIGELQNGLFCSRKSDIYWTAKGSE 60 Query: 90 ALLPTGVMLARFRSELQKHGYPLPGPKEAPLFQEKPDDATPSQAEQSLQVGVIVAQVLTN 149 ALLPTGVMLARFRSELQKHGYPLPGPKEAPLFQEKPDDATPSQAEQSLQVGVIVAQVLTN Sbjct: 61 ALLPTGVMLARFRSELQKHGYPLPGPKEAPLFQEKPDDATPSQAEQSLQVGVIVAQVLTN 120 Query: 150 LCLKGNASWTGEAYLRLEWQVFAPDQRKVIFKTVSEGVFQSRQASLEGAAPPIVAEAFSV 209 LCLKGNASWTGEAYLRLEWQVFAPDQRKVIFKTVSEGVFQSRQASLEGAAPPIVAEAFSV Sbjct: 121 LCLKGNASWTGEAYLRLEWQVFAPDQRKVIFKTVSEGVFQSRQASLEGAAPPIVAEAFSV 180 Query: 210 GVRNLLADPAFALALQTPYDRAAAAANASILPPSQPAAPGAGAGGAVNKPVPGPAVSIDN 269 GVRNLLADPAFALALQTPYDRAAAAANASILPPSQPAAPGAGAGGAVNKPVPG AVSIDN Sbjct: 181 GVRNLLADPAFALALQTPYDRAAAAANASILPPSQPAAPGAGAGGAVNKPVPGLAVSIDN 240 Query: 270 LPAAQPGGEMSKVIGQLRQGVVTVLTEGRSGTGFYIGQSGWLLSNQHVVGTARSVRVRLP 329 LPAAQPGGEMSKVIGQLRQGVVTVLTEGRSGTGFYIGQSGWLLSNQHVVGTARSVRVRLP Sbjct: 241 LPAAQPGGEMSKVIGQLRQGVVTVLTEGRSGTGFYIGQSGWLLSNQHVVGTARSVRVRLP 300 Query: 330 GGRELPAEVLRVDAARDVALLKTEPPGVRPLPLRLDEPGLGEDVYALGSPLGDTFNTTLT 389 GGRELPAEVLRVDAARDVALLKTEPPGVRPLPLRLDEPGLGEDVYALGSPLGDTFNTTLT Sbjct: 301 GGRELPAEVLRVDAARDVALLKTEPPGVRPLPLRLDEPGLGEDVYALGSPLGDTFNTTLT 360 Query: 390 RGILSGVRLLNGQEFLQSDVAILPGSSGGPLLDRSGQVVGITVAGLGARGMAGMNFFIPL 449 RGILSGVRLLNGQEFLQSDVAILPGSSGGPLLDRSGQVVGITVAGLGARGMAGMNFFIPL Sbjct: 361 RGILSGVRLLNGQEFLQSDVAILPGSSGGPLLDRSGQVVGITVAGLGARGMAGMNFFIPL 420 Query: 450 ASAVERLNLQLQQRPPKR 467 ASAVERLNLQLQQRPPKR Sbjct: 421 ASAVERLNLQLQQRPPKR 438 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 96.3 bits (238), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 65/164 (39%), Positives = 98/164 (59%), Gaps = 14/164 (8%) Query: 300 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 359 G+GF I G +L+N HVV A+ V V+L RE A+VL +D D+A+++ + + Sbjct: 126 GSGFIISPDGLILTNAHVVDGAQEVVVKLTDRREFKAKVLGIDKQSDIAVIRID---AKN 182 Query: 360 LP-LRLDEPG---LGEDVYALGSPLGDTFNTTLTRGILSG-VRLL---NGQEFLQSDVAI 411 LP +++ +P +G+ V A+GSP G F+ T T GI+S R L N F+Q+DVA+ Sbjct: 183 LPTVQIGDPSRVKVGQPVLAIGSPYG--FDNTATAGIISAKSRSLPDDNYVPFIQTDVAV 240 Query: 412 LPGSSGGPLLDRSGQVVGITVAGLGAR-GMAGMNFFIPLASAVE 454 PG+SGGPL D +GQV+GI G G++F IP+ A++ Sbjct: 241 NPGNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPIDVAMK 284 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 89.7 bits (221), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 62/167 (37%), Positives = 97/167 (58%), Gaps = 9/167 (5%) Query: 300 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 359 G+GF I G++++N HVV A V V L RE A+++ D DVA+LK + + Sbjct: 117 GSGFIISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPR 176 Query: 360 LPL-RLDEPGLGEDVYALGSPLGDTFNTTLTRGILSGVRLLNGQE--FLQSDVAILPGSS 416 L + D+ +GE V A+GSP G T+T GI+S G +Q+DVA+ PG+S Sbjct: 177 LNMGDSDKIRVGEWVLAIGSPFG--LENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNS 234 Query: 417 GGPLLDRSGQVVGITVAGLGAR--GMAGMNFFIPLASAVERLNLQLQ 461 GGPL++ G+V+GI + + +R G G++F +P+ A+ R+ QL+ Sbjct: 235 GGPLINLKGEVIGIN-SQIYSRSGGFMGISFAVPIDEAL-RVADQLK 279 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 86.3 bits (212), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 56/163 (34%), Positives = 94/163 (57%), Gaps = 15/163 (9%) Query: 300 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 359 G+G + G++L+N HVV A + V L GR+ A+V+ +D D+A++K + P + Sbjct: 114 GSGVIVSPQGYILTNNHVVEAADKIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPA 173 Query: 360 LPL-RLDEPGLGEDVYALGSPLGDTFNTTLTRGILS-------GVRLLNGQEFLQSDVAI 411 + L + +G+ V A+G+P G T+T GI+S G+ + F+Q+D AI Sbjct: 174 ITLGHPENSSVGDVVLAIGNPFG--VGQTVTMGIVSALGRNHLGINTF--ENFIQTDAAI 229 Query: 412 LPGSSGGPLLDRSGQVVGITVAGLGAR--GMAGMNFFIPLASA 452 PG+SGG L+D +G ++GI A + +R G G+ F IP+++A Sbjct: 230 NPGNSGGALVDTNGNLLGINTA-IYSRTGGNLGIGFAIPMSTA 271 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,519,836 Number of extensions: 67481 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 267 Number of HSP's successfully gapped: 4 Length of query: 467 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 378 Effective length of database: 1,198,455 Effective search space: 453015990 Effective search space used: 453015990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)