BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0386.p
         (422 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310489|ref|YP_003774581.1| glucarate dehydratase [Herbaspi...   867   0.0  
gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac...   231   8e-62

>gi|300310489|ref|YP_003774581.1| glucarate dehydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 455

 Score =  867 bits (2240), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 422/422 (100%), Positives = 422/422 (100%)

Query: 1   MLLNLSGAHGPYFTRNLVILTDSAGNTGVGEVPGGEGIRQTLEDARTLVVGQSIGNVQGI 60
           MLLNLSGAHGPYFTRNLVILTDSAGNTGVGEVPGGEGIRQTLEDARTLVVGQSIGNVQGI
Sbjct: 34  MLLNLSGAHGPYFTRNLVILTDSAGNTGVGEVPGGEGIRQTLEDARTLVVGQSIGNVQGI 93

Query: 61  LNRARTAFADRDTGGRGLQTFDLRIAIHAVTALEAALLDLLGKFLDVPVAALLGEGQQRD 120
           LNRARTAFADRDTGGRGLQTFDLRIAIHAVTALEAALLDLLGKFLDVPVAALLGEGQQRD
Sbjct: 94  LNRARTAFADRDTGGRGLQTFDLRIAIHAVTALEAALLDLLGKFLDVPVAALLGEGQQRD 153

Query: 121 EVEMLGYLFYVADRNKTDLPYLAAPDAQDDWTRLRHEAALTPEAVVRLAEAAYARYGFND 180
           EVEMLGYLFYVADRNKTDLPYLAAPDAQDDWTRLRHEAALTPEAVVRLAEAAYARYGFND
Sbjct: 154 EVEMLGYLFYVADRNKTDLPYLAAPDAQDDWTRLRHEAALTPEAVVRLAEAAYARYGFND 213

Query: 181 FKLKGGVMRGEEEIAAVTALAERFPKARITLDPNGGWLLKDAIRLCRDQHDVLAYAEDPC 240
           FKLKGGVMRGEEEIAAVTALAERFPKARITLDPNGGWLLKDAIRLCRDQHDVLAYAEDPC
Sbjct: 214 FKLKGGVMRGEEEIAAVTALAERFPKARITLDPNGGWLLKDAIRLCRDQHDVLAYAEDPC 273

Query: 241 GAEDGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAISLQSVDIPLADPHFWTMQGSV 300
           GAEDGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAISLQSVDIPLADPHFWTMQGSV
Sbjct: 274 GAEDGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAISLQSVDIPLADPHFWTMQGSV 333

Query: 301 RVAQMCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKITAIDTHWIWQDGQRLTREPLQ 360
           RVAQMCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKITAIDTHWIWQDGQRLTREPLQ
Sbjct: 334 RVAQMCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKITAIDTHWIWQDGQRLTREPLQ 393

Query: 361 IVGGKVQVPTKPGLGVELDMAQVEAAHQTYRNMGLGARDDAVAMQYLIPGWKFDPKKPCL 420
           IVGGKVQVPTKPGLGVELDMAQVEAAHQTYRNMGLGARDDAVAMQYLIPGWKFDPKKPCL
Sbjct: 394 IVGGKVQVPTKPGLGVELDMAQVEAAHQTYRNMGLGARDDAVAMQYLIPGWKFDPKKPCL 453

Query: 421 VR 422
           VR
Sbjct: 454 VR 455


>gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate
           lactonizing [Herbaspirillum seropedicae SmR1]
          Length = 424

 Score =  231 bits (589), Expect = 8e-62,   Method: Compositional matrix adjust.
 Identities = 156/419 (37%), Positives = 214/419 (51%), Gaps = 26/419 (6%)

Query: 2   LLNLSGAHGPYFTRNLVILTDSAGNTGVGEVPGGEGIRQTLEDARTLVVGQSIGNVQGIL 61
           LLN SG H PY  R+++ +    G  G+GE  G     + L+  +  +VG    N+ G+ 
Sbjct: 18  LLNASGIHEPYALRSIIEIETDNGYIGLGESYGDAPALKILDLVKERLVGLDPFNLNGLR 77

Query: 62  NRAR---TAFADRDTGGRGLQ--TFDLRIAIHAVTALEAALLDLLGKFLDVPVAALLGEG 116
              R    A A     G  L   +   +   +A +A E A LD   ++L+VP+  LLG G
Sbjct: 78  AIVRGVVAAMAPASNAGAELAPGSHASKAVSNAYSAFEVACLDAQARYLNVPLVDLLG-G 136

Query: 117 QQRDEVEMLGYLFYVADRNKTDLPYLAAPDAQDDWTRLRHEAALTPEAVVRLAEAAYARY 176
             R E+    YLF+   ++  D PY  APDA   W       AL+ E +V  A       
Sbjct: 137 AVRKEIPFSAYLFFKYAQH-IDSPY--APDA---WGE-----ALSEEQIVAQARKMIEEN 185

Query: 177 GFNDFKLKGGVMRGEEEIAAVTALAERFPKARITLDPNGGWLLKDAIRLCRDQHDVLAYA 236
           GF   KLK G +  E E+A + AL + FP A + +DPNG W L+ AIR+     D L Y 
Sbjct: 186 GFQSIKLKAGALAPEHEVACIKALRKAFPDAPLRIDPNGNWSLQTAIRMAELLGDDLQYY 245

Query: 237 EDPCGAEDGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAISLQSVDIPLADPHFW-T 295
           EDP        G E MAE  R TGL  ATNMV TD+ ++  +++L SV I LAD H+W  
Sbjct: 246 EDPT------PGLEGMAELHRRTGLPLATNMVVTDFDELRRSVALDSVQIVLADHHYWGG 299

Query: 296 MQGSVRVAQMCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKITAIDTHWIWQ--DGQR 353
           ++ + ++A+MC  +GL    HSN+H  +SL   +HVAAA      A DTH+ WQ  D + 
Sbjct: 300 LRDTQQLAKMCDVFGLGVSMHSNSHLGISLMAMSHVAAAVENLSYACDTHYPWQEPDEEV 359

Query: 354 LTREPLQIVGGKVQVPTKPGLGVELDMAQVEAAHQTYRNMGLGARDDAVAMQYLIPGWK 412
           +    L I  G VQ+   PGLGVE+D  Q+   HQ Y   G+  RDD   M+   P WK
Sbjct: 360 IQGGKLPIRNGCVQITDAPGLGVEVDQDQLRKLHQLYLECGIRQRDDVGQMRKYQPDWK 418


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.136    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,460,518
Number of extensions: 65428
Number of successful extensions: 201
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 196
Number of HSP's successfully gapped: 2
Length of query: 422
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 334
Effective length of database: 1,203,190
Effective search space: 401865460
Effective search space used: 401865460
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 132 (55.5 bits)