BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0385.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 880 0.0 gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 586 e-168 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 315 4e-87 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 310 1e-85 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 285 7e-78 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 880 bits (2274), Expect = 0.0, Method: Compositional matrix adjust. Identities = 442/447 (98%), Positives = 445/447 (99%) Query: 1 VNPSQSSAPAGLALTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAA 60 +NPSQSS PAGLALTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAA Sbjct: 1 MNPSQSSTPAGLALTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAA 60 Query: 61 FGLGAGLFFVGYALFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRF 120 FGLGAGLFFVGYALFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRF Sbjct: 61 FGLGAGLFFVGYALFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRF 120 Query: 121 LLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLH 180 LLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLH Sbjct: 121 LLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLH 180 Query: 181 GWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQAQRAANRPAH 240 GWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQ QRAANRPAH Sbjct: 181 GWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQEQRAANRPAH 240 Query: 241 VSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAI 300 VSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAI Sbjct: 241 VSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAI 300 Query: 301 AAMYLFAVLAARYKKPQFWVATTLVISAAGMAISAVAGPVIGFVAVCFAGIGFKAASSLF 360 AAMYLFAVLAARYKKPQFWVATTLVISAAGMAISAVAGPVIGFVAVCFAGIGFKAASSLF Sbjct: 301 AAMYLFAVLAARYKKPQFWVATTLVISAAGMAISAVAGPVIGFVAVCFAGIGFKAASSLF 360 Query: 361 WPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAA 420 WPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAA Sbjct: 361 WPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAA 420 Query: 421 VLVFLARTRPASPEGGGPAEAAVKHAA 447 VLVFLARTRPA+P+GGGPAEAAVKHAA Sbjct: 421 VLVFLARTRPAAPDGGGPAEAAVKHAA 447 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 586 bits (1511), Expect = e-168, Method: Compositional matrix adjust. Identities = 300/419 (71%), Positives = 350/419 (83%) Query: 14 LTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYA 73 L AV+KIK R+LPLFV MFI+NYIDRVNIGFVRSHL+TD+GIG AA+GLGAGLFFVGYA Sbjct: 26 LQQAVAKIKQRVLPLFVIMFIVNYIDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYA 85 Query: 74 LFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGV 133 LFEVPSN+LLQR+GA+AWLTRIM TWGL A MAFV SET+FY +RFLLG AEAGFFPGV Sbjct: 86 LFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGV 145 Query: 134 VYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLHGWQWMFIIEGGAS 193 +YYFTQWLP ERGRAMA+FLSGSA+ASILSGPI+G LL I+GGGL GWQWMFIIEG AS Sbjct: 146 IYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQIDGGGLQGWQWMFIIEGMAS 205 Query: 194 MVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQAQRAANRPAHVSALSLLRDPQIV 253 ++ C VW LDS P +AKWL+ ++R A+ I EQA+R ++PA VS LLRDPQI+ Sbjct: 206 VLLCGFVWFWLDSMPADAKWLTAQERSAITRAIVDEQAERMKHQPAQVSPRQLLRDPQIL 265 Query: 254 LFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYLFAVLAARY 313 +FCFIYF+I LTIYGATFWLP+II+KMG F+DFQVGLFNSIPW+++I AMY FA LAAR+ Sbjct: 266 IFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARF 325 Query: 314 KKPQFWVATTLVISAAGMAISAVAGPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIA 373 K Q W AT LVI+AAGM S V FV++CFA +GFKAASSLFWP+PQAYLDARIA Sbjct: 326 KHQQAWAATALVIAAAGMFASTFGNSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIA 385 Query: 374 AAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAAVLVFLARTRPAS 432 A V+ALINS+GNLGGF AP FG+LEQ+TGSIQGGL L+ SL+AA +VFLAR+ P + Sbjct: 386 AGVIALINSIGNLGGFVAPTAFGFLEQRTGSIQGGLMGLAITSLVAAGVVFLARSGPGA 444 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 315 bits (808), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 169/418 (40%), Positives = 251/418 (60%), Gaps = 12/418 (2%) Query: 19 SKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYALFEVP 78 SK+ WR+LPL ++ +Y+DRVN+GF + + DL +GLGAG+FF+GY +FEVP Sbjct: 22 SKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEVP 81 Query: 79 SNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGVVYYFT 138 SNM+L + GAR W+ RIM TWG+++ MA+V + FY MRFLLGVAEAGFFPGV+ Y T Sbjct: 82 SNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLT 141 Query: 139 QWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGG--GLHGWQWMFIIEGGASMVF 196 W P RG+ A+F++G A++ ++ GP++G ++ G G GWQWMFI+E S++ Sbjct: 142 YWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLIL 201 Query: 197 CVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQAQRAANRPAHVSALSLLRDPQIVLFC 256 V+V L + R+A WLS+E++ L A I+ E++Q+ H+S ++ + ++ L Sbjct: 202 GVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQK-----EHLSLGAMFANGRVWLGA 256 Query: 257 FIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYLFAVLAARYKKP 316 IYF + +YG +FWLPTIIK G + VGL +IP+ A AAM L A ++ Sbjct: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 Query: 317 QFWVATTLVISAAGMAISAVAG--PVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIAA 374 ++ VA I G+ +S G + ++ A +G LFW +P A+L AA Sbjct: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAA 376 Query: 375 AVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAAVLVFLARTRPAS 432 A +ALINSLGNL GF +P G+L+ T S G++ L+ + ++ +L T PA Sbjct: 377 AGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTL---TLPAK 431 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 310 bits (795), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 158/399 (39%), Positives = 244/399 (61%), Gaps = 8/399 (2%) Query: 19 SKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYALFEVP 78 K+ WRI+P + ++I Y+DRVN+GF + +S DLG FGLGAGLFF+GY LFEVP Sbjct: 23 KKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEVP 82 Query: 79 SNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGVVYYFT 138 SN+L+ + GAR W+ RIM TWG+++A FVQ+ T FY +RFLLG+AEAGF+PG++ Y T Sbjct: 83 SNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYLT 142 Query: 139 QWLPNHERGRAMAVFLSGSAIASILSGPITGALL--LIEGGGLHGWQWMFIIEGGASMVF 196 W P+H R + +AVF+SG +A IL P++G ++ + GGL GWQWMF+IE +++ Sbjct: 143 YWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLI 202 Query: 197 CVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQAQRAANRPAHVSALSLLRDPQIVLFC 256 V L LD+ + AKWL+ E++ +L A I + A + + ++++D ++ L C Sbjct: 203 GVATVLYLDNDVKSAKWLNDEEKASLQADIDGD----AKGKESKHGFGAIVKDARVWLMC 258 Query: 257 FIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYLFAVLAARYKKP 316 IYF+ + YG T W+PT++K G + ++GL ++IP+ AI AM L A R ++ Sbjct: 259 LIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRER 318 Query: 317 QFWVATTLVISAAGMAISAV--AGPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIAA 374 ++ + ++ G +A+ + ++ A G + LFW +P A+L AA Sbjct: 319 RWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAA 378 Query: 375 AVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALS 413 +A INS+GNL GF +P GYL+ T + G++ L+ Sbjct: 379 VGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLA 417 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 285 bits (728), Expect = 7e-78, Method: Compositional matrix adjust. Identities = 181/446 (40%), Positives = 252/446 (56%), Gaps = 30/446 (6%) Query: 2 NPSQSSAPAGLALTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAAF 61 N SQ SA + K+ W+ILP + ++I Y+DRVN+GF + + +DLG+ AA+ Sbjct: 3 NASQGSA---VQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAY 59 Query: 62 GLGAGLFFVGYALFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAF---------VQSE 112 GLGAG+FF+GY +FEVPSN++L R GA+ W+ RIM TWG++++ MAF V +E Sbjct: 60 GLGAGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNE 119 Query: 113 TSFYFMRFLLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALL 172 T FY +RFLLG +EAGFFPGV+ Y T W P R MAV L G+ + IL GPI+GAL+ Sbjct: 120 TVFYTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALM 179 Query: 173 LIEGGG--LHGWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQ 230 G G L GWQWMF+IE + + +V+ VL + AKWL+ E++ LA +AAE Sbjct: 180 QFMGSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAEN 239 Query: 231 AQRAANRPAHVSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGL 290 ++ N P SL P + +Y + + YG FW PTII+ G + +G+ Sbjct: 240 KEK-VNVPLK----SLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGM 294 Query: 291 FNSIPWIVAIAAMYLFAVLAARYKKPQ-FWVATTLVISAAGMAISAVAGPVIGFVAVCFA 349 ++IP+I+ AM A + K + + VA ++V AG+A+ + + + A Sbjct: 295 ISAIPYILGATAMVFITRHAEKAGKHRSYSVAVSMV---AGLAL-VLCTLFPHNIYITLA 350 Query: 350 GIGFKAASS-----LFWPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGS 404 GI S LFW P AY AAA +A+INS+GNLGGF P G L GS Sbjct: 351 GIAIAVGGSLTSIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGS 410 Query: 405 IQGGLFALSGASLLAAVLVFL-ARTR 429 + GL L GA LA VL+ L R R Sbjct: 411 PRYGLMMLGGALFLAGVLIALGGRKR 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.139 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,196,802 Number of extensions: 45716 Number of successful extensions: 358 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 309 Number of HSP's successfully gapped: 23 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)