BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0381.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/sign... 525 e-150 gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/sign... 102 4e-23 gi|300311601|ref|YP_003775693.1| glutamine ABC transporter perip... 92 4e-20 gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/sign... 92 4e-20 gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signa... 87 1e-18 >gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 258 Score = 525 bits (1353), Expect = e-150, Method: Compositional matrix adjust. Identities = 257/258 (99%), Positives = 258/258 (100%) Query: 1 MKPFHCLFKHLVGSVALAAAVATLGSAPAMAQASYNVGATSTGVPFTFLDIKTNKLQGMM 60 MKPFHCLFKHLVGSVALAAAVATLGSAPAMAQASYNVGATSTGVPFTFLDIKTNKLQGMM Sbjct: 1 MKPFHCLFKHLVGSVALAAAVATLGSAPAMAQASYNVGATSTGVPFTFLDIKTNKLQGMM 60 Query: 61 VDTVTAVGNKAGFAVNIQQTPFAALIPSLTGNKIDIIAAAMLKTEARAQIVDFSDPVYSY 120 VDTVTAVGNKAGF+VNIQQTPFAALIPSLTGNKIDIIAAAMLKTEARAQIVDFSDPVYSY Sbjct: 61 VDTVTAVGNKAGFSVNIQQTPFAALIPSLTGNKIDIIAAAMLKTEARAQIVDFSDPVYSY 120 Query: 121 GEGLFVNAQDGKSYKNLDDLKGEIVGAQVGTVFVDALNKKGGFKEVKTYDSVADMMRDVA 180 GEGLFVNAQDGKSYKNLDDLKGEIVGAQVGTVFVDALNKKGGFKEVKTYDSVADMMRDVA Sbjct: 121 GEGLFVNAQDGKSYKNLDDLKGEIVGAQVGTVFVDALNKKGGFKEVKTYDSVADMMRDVA 180 Query: 181 LGRIKAGFGDRPIVAYQLAQGANPQVRLVKEYQPTLMGDVCLVIRKGDPKLMERLNKAIA 240 LGRIKAGFGDRPIVAYQLAQGANPQVRLVKEYQPTLMGDVCLVIRKGDPKLMERLNKAIA Sbjct: 181 LGRIKAGFGDRPIVAYQLAQGANPQVRLVKEYQPTLMGDVCLVIRKGDPKLMERLNKAIA 240 Query: 241 ELKADGTLARIVEQWKLN 258 ELKADGTLARIVEQWKLN Sbjct: 241 ELKADGTLARIVEQWKLN 258 >gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 102 bits (253), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 77/250 (30%), Positives = 119/250 (47%), Gaps = 13/250 (5%) Query: 12 VGSVALAAAVATLGSAPAMAQASYNVGATSTGV-----PFTFLDIKTNKLQGMMVDTVTA 66 VG+ LA++++ SA + Q+ + G + PF F D KT +L G VD Sbjct: 13 VGAALLASSLSV--SAADLLQSVKSSGTLKVALEGNYPPFNFKDPKTGELTGFEVDVAKL 70 Query: 67 VGNKAGFAVNIQQTPFAALIPSLTGNKIDIIAAAMLKTEARAQIVDFSDPVYSYGEGLFV 126 + K G T ++ ++ L K D+I + T+ R + DFSDP L V Sbjct: 71 LAAKLGVKPVFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSSAQLIV 130 Query: 127 NAQDGKSYKNLDDLKGEIVGAQVGTVFVDALNKKGGFKEVKTYDSVADMMRDVALGRIKA 186 + + +K+L+DLKG+ +G GT F G +V+TY + + D+A GRI A Sbjct: 131 RKDEKREFKSLEDLKGKKLGLGQGTNFEQKAKAVPGI-DVRTYPGSPEYLADLAAGRIDA 189 Query: 187 GFGDRPIVAYQLAQGANPQVRLVKEYQPTLMGD-VCLVIRKGDPKLMERLNKAIAELKAD 245 D +V Y L P +K P D + + RKG+P+ LNKA+A++KAD Sbjct: 190 ALNDSLLVGYILKSTNLP----LKAGSPIGAVDKIGIPFRKGNPEFKAALNKALADIKAD 245 Query: 246 GTLARIVEQW 255 G+ E+W Sbjct: 246 GSFKAASEKW 255 >gi|300311601|ref|YP_003775693.1| glutamine ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 92.4 bits (228), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 86/259 (33%), Positives = 124/259 (47%), Gaps = 17/259 (6%) Query: 1 MKPFHCLFKHLVGSVALAAAVATLGSAPAMAQASYNVGATSTGVPFTFLDIKTNKLQGMM 60 MK F L G +L+ +A + +A A + VG +T P F +K K G Sbjct: 1 MKTFSKLL--CAGMASLSLLIAAVPAAHANGEV-IRVGTDATFPPMEF--VKDGKRTGFD 55 Query: 61 VDTVTAVGNKAGFAVNIQQTPFAALIPSLTGNKIDIIAAAMLKTEARAQIVDFSDPVYSY 120 V+ + A+ G V F LIP L N+ DI A+A+ T+ R ++V+FSDP Y Sbjct: 56 VELIEALAKTMGKKVQWIDIDFKGLIPGLVSNRFDIAASAIYMTDERRKVVNFSDPYYRG 115 Query: 121 GEGLFVNAQDGKSYKNLDDL-KGEIVGAQVGTVFVDALNKK-GGFKEVKTYDSVADMMRD 178 G + V +D S K +DL KG+ V QVGT V L G + V+ + A M Sbjct: 116 GLAVLVR-RDDNSIKVPEDLNKGKRVSVQVGTKSVGYLKDNFPGVERVEVEKNQA-MFDL 173 Query: 179 VALGRIKAGFGDRP-IVAYQLAQGANPQVRLV-KEYQPTLMGDVCLVIRKGDPKLMERLN 236 +A GR+ A RP V Y Q P VR++ K L G +RK D +L +LN Sbjct: 174 LATGRVNAVVTGRPAAVEYARTQ---PLVRVLDKGLTTELYG---FALRKSDTELTAQLN 227 Query: 237 KAIAELKADGTLARIVEQW 255 KA+ L+ +GT + ++W Sbjct: 228 KALQTLRMNGTYTALTDKW 246 >gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 271 Score = 92.0 bits (227), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 67/226 (29%), Positives = 112/226 (49%), Gaps = 11/226 (4%) Query: 35 YNVGATSTGVPFTFLDIKTNKLQGMMVDTVTAVGNKAGFAVNIQQTPFAALIPSLTGNKI 94 Y VG S PF+ + + + G +D + A+ K G V TPF L Sbjct: 49 YVVGVESAYAPFS-SENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNFLAQGDR 107 Query: 95 DIIAAAMLKTEARAQIVDFSDPVYSYGEGLFVNAQDGKSYKNLDDLKGEIVGAQVGT--- 151 D++ +A+ T+ R + V FS+P + + + + A D K K ++DLK VG Q T Sbjct: 108 DLLISAITITDERKKSVAFSEPYFVATQTIALPAADTKVSK-MEDLKPLTVGTQSATSGD 166 Query: 152 -VFVDALNKKGGFKEVKTYDSVADMMRDVALGRIKAGFGDRPIVAYQLAQGANPQVRLVK 210 + L K ++K +DS ++++ G + A D P+V +A N ++R V Sbjct: 167 ELVQQVLGKNSA--KIKRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPNSKLRTVT 224 Query: 211 EYQPTL-MGDVCLVIRKGDPKLMERLNKAIAELKADGTLARIVEQW 255 + P+ D + +RK DP+L+ ++NK +AE+KADG+ A I Q+ Sbjct: 225 D--PSFPKEDYGIAVRKDDPELLAKINKGLAEMKADGSFAAISAQY 268 >gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 87.4 bits (215), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 70/240 (29%), Positives = 112/240 (46%), Gaps = 12/240 (5%) Query: 19 AAVATLGSAPAMAQASYNVGATSTG-VPFTFLDIKTNKLQGMMVDTVTAVGNKAGFAVNI 77 A++ L A A+A V A T VPF F D N+ G +D A+ G + Sbjct: 11 ASLVALSVVGASARAETLVVACDTAFVPFEFKD--RNQYVGFDIDLWDAIAKDIGVTYKL 68 Query: 78 QQTPFAALIPSLTGNKIDIIAAAMLKTEARAQIVDFSDPVYSYGEGLFVNAQDGKSYKNL 137 Q F +IP+L +D+ A + + R +++DFSD Y G L V G S K++ Sbjct: 69 QPMDFNGIIPALQTKNVDVALAGITIKDERKKVIDFSDGYYDSGFSLMVPV--GSSIKSV 126 Query: 138 DDLKGEIVGAQVGTVFVDALNKKGGFK--EVKTYDSVADMMRDVALGRIKAGFGDRPIVA 195 DL G+ + + GT D K FK E+ + ++ + ++ GR+ A D P V Sbjct: 127 ADLAGKALAVKSGTSAFD--YAKANFKATELHQFPNIDNAYLELQTGRVDAAMHDTPNVL 184 Query: 196 YQLAQGANPQVRLVKEYQPTLMGDVCLVIRKGDPKLMERLNKAIAELKADGTLARIVEQW 255 Y +A + ++V + + KG P L+ ++N A+A++KADG A I +W Sbjct: 185 YYIATAGKGRAKVVG--TQMMAHQYGIGFPKGSP-LVPKVNAALAKIKADGRYAEIYRKW 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 721,180 Number of extensions: 29129 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 120 Number of HSP's successfully gapped: 12 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)