BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0368.p (252 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehyd... 501 e-143 gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain)... 307 6e-85 gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehyd... 250 8e-68 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 91 7e-20 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 91 1e-19 >gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 501 bits (1289), Expect = e-143, Method: Compositional matrix adjust. Identities = 252/252 (100%), Positives = 252/252 (100%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSS 60 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSS Sbjct: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSS 60 Query: 61 EEDAQAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMAR 120 EEDAQAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMAR Sbjct: 61 EEDAQAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMAR 120 Query: 121 FAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSG 180 FAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSG Sbjct: 121 FAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSG 180 Query: 181 IRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNGET 240 IRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNGET Sbjct: 181 IRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNGET 240 Query: 241 IRLDGAIRMQPK 252 IRLDGAIRMQPK Sbjct: 241 IRLDGAIRMQPK 252 >gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain) dehydrogenase type II oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 307 bits (786), Expect = 6e-85, Method: Compositional matrix adjust. Identities = 158/254 (62%), Positives = 193/254 (75%), Gaps = 2/254 (0%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGV--FVRCDV 58 M ++ V+++TGAASGLGAATA + AG K VLAD+N AGE + A LG FV DV Sbjct: 1 MAVKQGVYVVTGAASGLGAATAEQLIRAGAKVVLADVNPVAGEKMQATLGANARFVLTDV 60 Query: 59 SSEEDAQAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNM 118 +SE AQ V A ++G++ GLVNCAGIAP EKL+GK G H + FM+ LNINLVGSFNM Sbjct: 61 TSETSAQEVFGVASSMGAVVGLVNCAGIAPGEKLLGKGGVHRLDSFMRALNINLVGSFNM 120 Query: 119 ARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSR 178 AR AA+ ++ A GERGVIIHTAS+AA+DGQ+GQ AYAASKG +V+MTLPMAREL+R Sbjct: 121 ARLAAQHISAGPADATGERGVIIHTASIAAFDGQMGQVAYAASKGGVVSMTLPMARELAR 180 Query: 179 SGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNG 238 GIRV+TIAPGI ETPMML MP+EV +SL + VPFPPRLG+ +E+A L I+EN LNG Sbjct: 181 HGIRVVTIAPGIIETPMMLGMPEEVLESLSRNVPFPPRLGKPSEFAGLVMHILENRYLNG 240 Query: 239 ETIRLDGAIRMQPK 252 E IR+DGA+RM K Sbjct: 241 EVIRMDGALRMSAK 254 >gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 250 bits (638), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 132/254 (51%), Positives = 184/254 (72%), Gaps = 2/254 (0%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSS 60 M+++G ++TG ASGLGA T R + AG + + D+N EA + LA EL VRCD++ Sbjct: 1 MQLQGQAALVTGGASGLGAETVRQLVQAGARVSILDVNMEAAQALADELRCHAVRCDITD 60 Query: 61 EEDAQAVVRAAEAL-GSLRGLVNCAGIAPAEKLVGKSG-PHTMALFMKTLNINLVGSFNM 118 + + ++AA+ G+ R L+NCAGI A+++VGK G P + F + +N+NL+G+FN+ Sbjct: 61 SDSVETALQAAQQTNGAPRILINCAGIGGAKRMVGKDGKPMPLEDFSRIVNVNLIGTFNV 120 Query: 119 ARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSR 178 R AA MA + A+ EGERGVI+ TASVAA+DGQ+GQ YAASKG I AMTLP+AR+L++ Sbjct: 121 IRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQ 180 Query: 179 SGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNG 238 GIRV+TIAPG+F TP++ +P+EV+ SL ++PFP RLG+A EYAQLA I N+ LNG Sbjct: 181 YGIRVVTIAPGLFLTPLLYKLPEEVQQSLASSIPFPKRLGKAEEYAQLALHIATNLSLNG 240 Query: 239 ETIRLDGAIRMQPK 252 E IRLDGA+R+ P+ Sbjct: 241 EVIRLDGALRLAPR 254 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 91.3 bits (225), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 86/264 (32%), Positives = 122/264 (46%), Gaps = 40/264 (15%) Query: 2 RIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFV------- 54 R EG V I+TGAASG+G ATAR + G + +LAD + AA LG VF Sbjct: 11 RFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRD-------AAALGKVFDSLPPERT 63 Query: 55 ---RCDVSSEEDAQAVVR-AAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNI 110 DVS E + +V A E G L LV+ AG+ AE V + P T N+ Sbjct: 64 AARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVF-AEGNVTEVSPEDWHRVQAT-NV 121 Query: 111 NLVGSFNMARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTL 170 N G F AR A + E RG I++ ASV+ +AY ASKGA+ +T Sbjct: 122 N--GVFYGAREALPHL-------EKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTR 172 Query: 171 PMARELSRSGIRVMTIAPGIFETPMMLAMPQE--VRDSLGQAVPFPPRLGRAAEYAQLAR 228 MA + R G+R+ + P + T M M + + D + + LGR A+ ++A Sbjct: 173 AMALDFGRKGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIA----LGRGADPLEIAA 228 Query: 229 TII-----ENVMLNGETIRLDGAI 247 I + +NG + +DG + Sbjct: 229 VITFLASPDASFVNGVNLPVDGGL 252 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 76/248 (30%), Positives = 122/248 (49%), Gaps = 20/248 (8%) Query: 7 VFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRC-----DVSSE 61 V ++TG A G+GA+ ++ +AA G ++AD+N +A A L R D+ S Sbjct: 10 VALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGSP 69 Query: 62 ED-AQAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMAR 120 E A A + G LVN AG+A + + + +++T+N+N+ G + Sbjct: 70 ESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFL----DYPLENWLQTMNVNVTGVLLAGQ 125 Query: 121 FAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSG 180 AA M + + G I++ +S++ G+TAY SK A++ +T MA EL++ G Sbjct: 126 HAARLMVKQ------QWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYG 179 Query: 181 IRVMTIAPGIFETPMMLAMPQE-VRDSLGQAVPFPPRLGRAAEYAQLARTII--ENVMLN 237 I V ++APG +TPM AM E R+S + VP R G E A + E+ + Sbjct: 180 ITVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPM-RRYGTTDEIASAVCYLAAEESAYIT 238 Query: 238 GETIRLDG 245 G I +DG Sbjct: 239 GHVIPVDG 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 657,886 Number of extensions: 24764 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 42 Length of query: 252 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 169 Effective length of database: 1,226,865 Effective search space: 207340185 Effective search space used: 207340185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)