BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0320.p (529 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309489|ref|YP_003773581.1| NAD-dependent dehydrogenase [He... 1063 0.0 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 72 1e-13 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 69 1e-12 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 63 5e-11 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 60 6e-10 >gi|300309489|ref|YP_003773581.1| NAD-dependent dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 529 Score = 1063 bits (2748), Expect = 0.0, Method: Compositional matrix adjust. Identities = 527/529 (99%), Positives = 529/529 (100%) Query: 1 VSFNILGHNYIGGQRSGQGDVALHSVDATTGALFETPFLTATDKEVAAAVHAAEQAYPLY 60 +SFNILGHNYIGGQRSGQGDVALHSVDATTGALFETPFLTATDKEVAAAVHAAEQAYPLY Sbjct: 1 MSFNILGHNYIGGQRSGQGDVALHSVDATTGALFETPFLTATDKEVAAAVHAAEQAYPLY 60 Query: 61 RATTSEQRAQFLEAIADEIDALGDDFLAAVARETALPATPRLAGERARTSGQMRLFAKVV 120 RATTSEQRAQFLEAIADEIDALGDDFLAAVARETALPATPRLAGERARTSGQMRLFAKVV Sbjct: 61 RATTSEQRAQFLEAIADEIDALGDDFLAAVARETALPATPRLAGERARTSGQMRLFAKVV 120 Query: 121 RRGDFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPLAFSVAGGDTAAALAA 180 RRGDFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPLAFSVAGGDTAAALAA Sbjct: 121 RRGDFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPLAFSVAGGDTAAALAA 180 Query: 181 GCPVVFKAHSGHLVTSELVADAIERAVKKTGMPAGTFNMIYGDRVGAQLVKSAGIQAVGF 240 GCPVVFKAHSGHLVTSELVADAIERAVKKTGMPAGTFNMIYGDRVGAQLVKSAGIQAVGF Sbjct: 181 GCPVVFKAHSGHLVTSELVADAIERAVKKTGMPAGTFNMIYGDRVGAQLVKSAGIQAVGF 240 Query: 241 TGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMPEALKLRGDAIAKDLAGSVTVGVG 300 TGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMPEALKLRGDAIAKDLAGSVTVGVG Sbjct: 241 TGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMPEALKLRGDAIAKDLAGSVTVGVG 300 Query: 301 QLCTSPGLLLGVRSPELTAFIEKLSAAFGGTNPATMLNSGGLTHYNGGVARLTQLPGVKV 360 QLCTSPGLLLGVRSPELT+FIEKLSAAFGGTNPATMLNSGGLTHYNGGVARLTQLPGVKV Sbjct: 301 QLCTSPGLLLGVRSPELTSFIEKLSAAFGGTNPATMLNSGGLTHYNGGVARLTQLPGVKV 360 Query: 361 IATGGTSYTQAVPHLFKADAALLFSKEAPLEEEVFGPSTVIVELESREQLLDFAAKMNGQ 420 IATGGTSYTQAVPHLFKADAALLFSKEAPLEEEVFGPSTVIVELESREQLLDFAAKMNGQ Sbjct: 361 IATGGTSYTQAVPHLFKADAALLFSKEAPLEEEVFGPSTVIVELESREQLLDFAAKMNGQ 420 Query: 421 LTATLQAEIGDLQGNQDLIAILEQKAGRLLLNGFPTGVEVCDAMVHGGPYPATSDARGTS 480 LTATLQAEIGDLQGNQDLIAILEQKAGRLLLNGFPTGVEVCDAMVHGGPYPATSDARGTS Sbjct: 421 LTATLQAEIGDLQGNQDLIAILEQKAGRLLLNGFPTGVEVCDAMVHGGPYPATSDARGTS 480 Query: 481 VGSLAIERFLRPVCYQNYPDAMLPAALQNANPLGLMRLVDGEQTRATVG 529 VGSLAIERFLRPVCYQNYPDAMLPAALQNANPLGLMRLVDGEQTRATVG Sbjct: 481 VGSLAIERFLRPVCYQNYPDAMLPAALQNANPLGLMRLVDGEQTRATVG 529 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 71.6 bits (174), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 80/282 (28%), Positives = 126/282 (44%), Gaps = 31/282 (10%) Query: 49 AVHAAEQAYPLYRATTSEQRAQFLEAIADEIDALGDDFLAAVARETALPATPRLAGERAR 108 A+ AA + + +R ++ +R++ + AD + ++ VAR + LA + Sbjct: 47 ALEAASKGFETWRNMSAFERSKIMRRAADLLRERAEE----VARLMTMEQGKPLAEAKME 102 Query: 109 TSG---QMRLFAKVVRRGDFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPL 165 T G + FA+ RR YG + +P + K VGPVA F NFPL Sbjct: 103 TLGAADTIDWFAEEARRT--YGRLVPARVPGAYQM------VIKEPVGPVAAFTPWNFPL 154 Query: 166 AFSVAGGDTAAALAAGCPVVFKAHSGHLVT-SELVADAIERAVKKTGMPAGTFNMIYG-- 222 V +AALAAGC ++ KA + +EL+ RA G+PAG N++YG Sbjct: 155 NQVVR--KLSAALAAGCSIIVKAPEETPASPAELI-----RAYADAGVPAGVINLVYGVP 207 Query: 223 DRVGAQLVKSAGIQAVGFTGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMPEALKL 282 + L+ I+ + FTGS G+ L +A + + E+ P ++ +A Sbjct: 208 AEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRATM--ELGGHAPAMVFDDA--- 262 Query: 283 RGDAIAKDLAGSVTVGVGQLCTSPGLLLGVRSPELTAFIEKL 324 D AK LA + GQ+C SP L V+ AF++K Sbjct: 263 DVDVAAKMLATAKFRNAGQVCVSPTRFL-VQKGVFNAFVDKF 303 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 68.6 bits (166), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 127/478 (26%), Positives = 188/478 (39%), Gaps = 71/478 (14%) Query: 9 NYIGGQRSG--QGDVALHSVDATTGALF-ETPFLTATDKEVAA-AVHAAEQAYPLYRATT 64 N+IGG+ +G+ ++ G F E +A D E+A A HAA++++ T+ Sbjct: 21 NFIGGKFVPPVKGEY-FENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWG---KTS 76 Query: 65 SEQRAQFLEAIADEIDALGDDFLAAVARETALPATPRLAGERARTSGQMRLFAKVVRRGD 124 +RA L IAD ++A + A + P +A + R FA VR + Sbjct: 77 PTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQE 136 Query: 125 FYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGA---SNFPLAFSVAGGDTAAALAAG 181 ID D Y P+ V G NFP+ +V A ALAAG Sbjct: 137 GSICPIDN-----------DTYAYHFH-EPLGVVGQIIPWNFPILMAV--WKLAPALAAG 182 Query: 182 CPVVFK----AHSGHLVTSELVADAIERAVKKTGMPAGTFNMI--YGDRVGAQLVKSAGI 235 VV K + +V EL+AD I P G N++ +G G L + I Sbjct: 183 NCVVLKPAEQTPASIMVLIELIADLI---------PPGVVNIVQGFGVEAGKPLASNKRI 233 Query: 236 QAVGFTGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMPEALKLRGDAIAKDLAGSV 295 + FTG GR + M A IPV E+ +P I + L D K L G Sbjct: 234 AKIAFTGETTTGRLI--MQYASQNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFA 291 Query: 296 TVGV--GQLCTSPGLLLGVRSPELTAFIE---KLSAAFGGTNP---ATMLNSGGLTHYNG 347 + G++CT P +L V+ FIE K AA NP +TM+ + Sbjct: 292 MFALNQGEVCTCPSRVL-VQESIYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLE 350 Query: 348 GVARLTQL---PGVKVIATGG--------TSYTQAVPHLFKADAALLFSKEAPLEEEVFG 396 + + G KV+A GG S P +F+ + +K +EE+FG Sbjct: 351 KILSYIDIGKQEGAKVLAGGGREELGGDLASGYYVKPTVFQGN-----NKMRIFQEEIFG 405 Query: 397 PSTVIVELESREQLLDFAAKMNGQLTATLQAEIGDLQGNQDLIAILEQKAGRLLLNGF 454 P + + E+ L A N L L A + G + E +AGR+ N + Sbjct: 406 PVVSVTTFKDEEEALAIA---NDTLYG-LGAGLWTRDGTRAFRMGREIQAGRVWTNCY 459 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 63.2 bits (152), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 87/324 (26%), Positives = 139/324 (42%), Gaps = 31/324 (9%) Query: 10 YIGGQR--SGQGD-VALHSVDATTGALFETPFLTATDKEVAAAVHAAEQAYPLYRATTSE 66 ++ GQ + QG+ +A+H+ A L P + A E A+ AA A+P ++ T++ Sbjct: 15 FVNGQWCDADQGERLAVHN-PANGELLGHVPLMGAA--ETRRAIEAANAAWPAWKKKTAK 71 Query: 67 QRAQFLEAIADEIDALGDDFLAAVARETALP-ATPRLAGERARTSGQMRLFAKVVRRGDF 125 +R+ L + + A DD + E P A R GE + + FA+ +R Sbjct: 72 ERSAILRRWYELMLANTDDLALIMTAEQGKPLAEAR--GEIGYAASFIEWFAEEGKRT-- 127 Query: 126 YGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPLAFSVAGGDTAAALAAGCPVV 185 YG I + P P I K +G A NFP A ALAAGCP+V Sbjct: 128 YGDTIPS------PSPSNRIVVIKEAIGVCAAITPWNFPAAMITR--KAGPALAAGCPMV 179 Query: 186 FKAHSGHLVTSELVADAIERAVKKTGMPAGTFNMIYG--DRVGAQLVKSAGIQAVGFTGS 243 K ++ +A ERA G+PAG F+++ G +G ++ + ++ + FTGS Sbjct: 180 LKPAEATPFSALALAVLAERA----GIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGS 235 Query: 244 LRGGRALCDMAAARPQPIPVFAEMSSINPIILMPEALKLRGDAIAKDLAGSVTVGVGQLC 303 G+ L +A+ + + + E+ P I+ +A DA + S GQ C Sbjct: 236 TGVGKLLMQQSASSIKKLSL--ELGGNAPFIVFDDA---DLDAAVEGAIASKYRNAGQTC 290 Query: 304 TSPGLLLGVRSPELTAFIEKLSAA 327 + V+ AF KL A Sbjct: 291 VCANRIY-VQDGVYDAFAAKLVEA 313 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 59.7 bits (143), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 111/443 (25%), Positives = 180/443 (40%), Gaps = 46/443 (10%) Query: 6 LGHNYIGGQ-RSGQG-DVALHSVDATTGALFETPFLTATDKEVAAAVHAAEQAYPLYRAT 63 L H YI G+ + QG + A AT + E A D V AV AA +A P + AT Sbjct: 15 LDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAAD--VDQAVQAARRAIPAWAAT 72 Query: 64 TSEQRAQFLEAIADEIDALGDDFLAAVARETALPATPRLAGERARTSGQMRLFAKVVRRG 123 + +RA L + + + F A+ E P + + +R+ + Sbjct: 73 SPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVARDNLADY 132 Query: 124 DFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPLAFSVAGGDTAAALAAGCP 183 F R TA+ R+P +G A+ N+P+ A ALAAGC Sbjct: 133 PFIRPRGTTAI-VREP------------IGVCALITPWNWPIYQITA--KVGPALAAGCT 177 Query: 184 VVFKAHSGHLVTSELVADAIERAVKKTGMPAGTFNMIYGD--RVGAQLVKSAGIQAVGFT 241 VV K +++ L A+ + A G+PAG FN++ G VGA L + + T Sbjct: 178 VVLKPSELSPLSALLFAEIVHEA----GVPAGVFNLVNGSGAEVGAALSAHPQVDMISIT 233 Query: 242 GSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMPEALKLRGDAIAKDLAGSVTVGVGQ 301 GS R G + AA + V E+ +P +++P+A R AI +A ++ +GQ Sbjct: 234 GSTRAGVLVAQAAAVTVK--RVAQELGGKSPNLVLPDADLER--AIPPGVAAALR-NMGQ 288 Query: 302 LCTSPGLLLGVRSPELTAFIEKLSAAFGGTNPATMLNSGGLTH----YNGGVARLTQL-- 355 C++P L+ R+ A + +L+ A N TH R+ Q+ Sbjct: 289 SCSAPTRLIVPRA--ALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIE 346 Query: 356 ----PGVKVIATGGTSYTQAVPHLFKADAALLFSKEAPL---EEEVFGPSTVIVELESRE 408 G +++A GG + + A + + +EE+FGP I+ ++ E Sbjct: 347 AGLQDGARLLA-GGPGRPDGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVE 405 Query: 409 QLLDFAAKMNGQLTATLQAEIGD 431 + + A L A +Q D Sbjct: 406 EAIAIANDTVYGLGAHVQGRDKD 428 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,685,228 Number of extensions: 74872 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 290 Number of HSP's successfully gapped: 8 Length of query: 529 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 439 Effective length of database: 1,193,720 Effective search space: 524043080 Effective search space used: 524043080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)