BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0319.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 828 0.0 gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 563 e-162 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 515 e-147 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 354 8e-99 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 100 3e-22 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 828 bits (2138), Expect = 0.0, Method: Compositional matrix adjust. Identities = 403/404 (99%), Positives = 403/404 (99%) Query: 1 MTPFIRRAALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMA 60 MTPFIRRAALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMA Sbjct: 1 MTPFIRRAALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMA 60 Query: 61 IADAGGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAG 120 IADAGGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAG Sbjct: 61 IADAGGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAG 120 Query: 121 EKKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAF 180 EKKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAF Sbjct: 121 EKKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAF 180 Query: 181 GTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAI 240 GTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAI Sbjct: 181 GTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAI 240 Query: 241 KAANEFGVNKTMKLAGLLIFINDTHSLGLPLTQGMYLTDGWYWDLNDQTRAWANRYFMKM 300 KAANEFGVNKTMKLAGLLIFINDTHSLGL LTQGMYLTDGWYWDLNDQTRAWANRYFMKM Sbjct: 241 KAANEFGVNKTMKLAGLLIFINDTHSLGLSLTQGMYLTDGWYWDLNDQTRAWANRYFMKM 300 Query: 301 KKMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVH 360 KKMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVH Sbjct: 301 KKMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVH 360 Query: 361 DMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 DMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK Sbjct: 361 DMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 563 bits (1451), Expect = e-162, Method: Compositional matrix adjust. Identities = 264/378 (69%), Positives = 310/378 (82%) Query: 27 QAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIADAGGAINGKKVELVFADHQNKADI 86 Q +GD IKIGFITD+SG Y+D DGQGG EAI+MAIAD GGAINGKK+EL+ ADHQNKAD+ Sbjct: 25 QISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADV 84 Query: 87 AANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTI 146 AA+KAREWFD+ GVDML+GG NS +LA KVA EKKKV++ +GAG+ R TNEEC+ YT+ Sbjct: 85 AASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTV 144 Query: 147 QYAYDTTALARGTGAAITRQGGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVP 206 YAYDT ALA+GTG+A+ +QGGKSW+FLTADYAFG SLE DT+ VVK++GG VVGA + P Sbjct: 145 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHP 204 Query: 207 LATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFINDTHS 266 L SDFSSFLLQAQSSKAQI+GLA AGGD IN++KAANEFG+ KT KLAGLL+FIND HS Sbjct: 205 LGASDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHS 264 Query: 267 LGLPLTQGMYLTDGWYWDLNDQTRAWANRYFMKMKKMPSMFQAGDASAVGQYLKAVKAVG 326 L L T+G+YLTD WYWD N++TRA+A RYF KKMPS QA D SA QYLK VK +G Sbjct: 265 LTLKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKKMPSSLQAADYSAALQYLKVVKEIG 324 Query: 327 STDADKVMDYLRKNRINDFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTI 386 + D+DKVM LRK + ND + NG +R DGR++HDMYL +VK SESK PWDYYKLVQTI Sbjct: 325 TDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTI 384 Query: 387 PGEQAYMTKAESKCALWK 404 P EQA+ TKAESKCA WK Sbjct: 385 PAEQAWTTKAESKCAAWK 402 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 515 bits (1326), Expect = e-147, Method: Compositional matrix adjust. Identities = 247/403 (61%), Positives = 309/403 (76%), Gaps = 4/403 (0%) Query: 2 TPFIRRAALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAI 61 TP R AA A A GAA+ AQ + D ++IGFITDMSG YAD DG GG+EAIRMA+ Sbjct: 5 TPLRRLAAACAIAGIFSGAAL---AQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMAV 61 Query: 62 ADAGGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGE 121 AD GG + GK +E++ ADHQNKADIAA +AREW D G+DML+GGVNSA +L+M KV E Sbjct: 62 ADFGGTVLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMAE 121 Query: 122 KKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAFG 181 KK+V+I++GAGT R TNEEC YT+ Y YDT ALA+GT +A+ +QGGKSW+FL ADYAFG Sbjct: 122 KKRVYINIGAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAFG 181 Query: 182 TSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIK 241 SL DTS VVK++GG VVG+ + P +SD SSFLLQAQ+SKAQILGLA AG D +N+IK Sbjct: 182 HSLANDTSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSIK 241 Query: 242 AANEFGVNKTMKLAGLLIFINDTHSLGLPLTQGMYLTDGWYWDLNDQTRAWANRYFMKMK 301 AA EFGV KTMKL GLL+ IND H+LGL +G+ +TD WYWD ++ +R +A R+F KMK Sbjct: 242 AAKEFGVTKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKMK 301 Query: 302 KMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVHD 361 KMPS QA SA YLKAV+A+G+ D+ KVM L+K RI+DF+ + G +R DGR +HD Sbjct: 302 KMPSTHQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDFY-NKGSIRADGRNIHD 360 Query: 362 MYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 MYL +VK P+ESK+PWDY KL++TIPGEQA+ + A+SKC+L K Sbjct: 361 MYLYQVKSPAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLK 403 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 354 bits (908), Expect = 8e-99, Method: Compositional matrix adjust. Identities = 189/403 (46%), Positives = 258/403 (64%), Gaps = 5/403 (1%) Query: 6 RRAALSATACAL-LGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIADA 64 R +A AC + L A +P+ +T+KIG +TDMSG Y+ GQG V A +MA+ D Sbjct: 8 RSLTAAAVACTVSLAATAIPAWAQDANTVKIGVLTDMSGPYSAMGGQGSVVAAKMAVEDC 67 Query: 65 -GGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKK 123 G K+E++ AD+QNK D+ +AREW DRD V+ + NS+ +LA+ K+ +K Sbjct: 68 LKNECKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAVQKLIKDKG 127 Query: 124 KVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAFGTS 183 + + G T T EC + +DT + A G AA+T+ G KSWYF+T DYAFG S Sbjct: 128 GIAMYSGPATGALTTAECAPNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHS 187 Query: 184 LEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAA 243 LEKD S+VVK+NGG VVG+ R PL SDFSSFLLQAQ+SKAQ++GLA G DV+NAIK A Sbjct: 188 LEKDASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQA 247 Query: 244 NEFGV-NKTMKLAGLLIFINDTHSLGLPLTQGMYLTDGWYWDLNDQTRAWANRYFMKMKK 302 F V K +L LL+F++D H+LGL QG+ TDG+YWD ++QTRA++ R+ + K Sbjct: 248 RSFAVGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAFSKRFESQFKN 307 Query: 303 M-PSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVHD 361 + P+M QAG S+V Q+LKA A ++D V +R+ I D N +RPDGR++HD Sbjct: 308 IKPTMVQAGVYSSVYQFLKARAAAKTSDWKVVAQKMREIPIADAVMRNASIRPDGRVIHD 367 Query: 362 MYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 MYL +VK P+ESK PWDY KL+ IP +QA+ ++ C L K Sbjct: 368 MYLYQVKTPAESKGPWDYLKLLSVIPAQQAF-RPMDAACPLVK 409 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 99.8 bits (247), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 107/409 (26%), Positives = 176/409 (43%), Gaps = 27/409 (6%) Query: 6 RRAALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIADAG 65 RR L +A A GA A ++I G MSG +A +G+ +++A+ G Sbjct: 4 RRHFLKLSALAAPGALSYHDVFAQAESIAFGCPVPMSGPFAA-NGKFADLGMKLALQKYG 62 Query: 66 GAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKV 125 + GK V D + K A K +E ++ G GG+ S +LAMGK A + V Sbjct: 63 KVL-GKSTSYVNLDTEGKPATAVRKMQESIEK-GTRFFAGGILSGEALAMGKEADKAGAV 120 Query: 126 FISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQ--GGKSWYFLTADYAFGTS 183 FI+ AG T +CN T +++ T T + + K WY +T Y FG Sbjct: 121 FITT-AGADEITGSDCNRATFRWSVPTFGAIEQTVRPLLEKMPNAKRWYTITPQYVFGEG 179 Query: 184 LEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAA 243 L + K G VG + L +FS +L A ++K +L L G + ++ A Sbjct: 180 LLSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTNAIAAKPDVLLLLNFGSQSSDTLRQA 239 Query: 244 NEFGV--NKTMKLA---GLLIFINDTHSLGLPLTQGMYLTDGWYWDLNDQTRAWANRYFM 298 FG+ N T+ +A GL F +LG + G+Y YW D A N+ F+ Sbjct: 240 VSFGMKNNCTILMAWASGLEQF----ETLGADICDGVYF-GAQYWHAID---APFNKEFV 291 Query: 299 K-----MKKMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVR 353 K +K P+ AG L A+ STD V+ + + S + + Sbjct: 292 KLVNDNLKIDPNYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYEGLTGSEEIRK 351 Query: 354 PDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCAL 402 D +++ + YL++ K ++ DY +V + ++++ +++C L Sbjct: 352 GDHQVIKNYYLLKGKAKAKMANKNDYADIVSS---GKSFLPLEKTQCKL 397 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,242,377 Number of extensions: 52550 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 11 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)