BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0301.p (470 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase... 936 0.0 gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillu... 73 5e-14 gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Her... 69 1e-12 gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptid... 57 3e-09 >gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase [Herbaspirillum seropedicae SmR1] Length = 470 Score = 936 bits (2419), Expect = 0.0, Method: Compositional matrix adjust. Identities = 468/470 (99%), Positives = 470/470 (100%) Query: 1 VSRGGIRPWLAGLALLLATAAVQSQPITERARQKQAAEKERADLQQRLANLKRDIDHTET 60 +SRGGIRPWLAGLALLLATAAVQSQPITERARQKQAAEKERADLQQRLANLKRDIDHTET Sbjct: 1 MSRGGIRPWLAGLALLLATAAVQSQPITERARQKQAAEKERADLQQRLANLKRDIDHTET 60 Query: 61 AKDNAADALAASEQAISKANRSLRDLAQEQQETEARLNQLIRQLNELTALVNAQQAQLSK 120 AKDNAADALAASEQAISKANRSLRDLAQEQQ+TEARLNQLIRQLNELTALVNAQQAQLSK Sbjct: 61 AKDNAADALAASEQAISKANRSLRDLAQEQQQTEARLNQLIRQLNELTALVNAQQAQLSK 120 Query: 121 LLREQYVAGNEDRIKLLLSGDNPNRINRELQYMGYVSQAQAKLIETLRANQQAIRANKAD 180 LLREQYVAGNEDRIKLLLSGDNPNRINRELQYMGYVSQAQAKLIETLRANQQAIRANKAD Sbjct: 121 LLREQYVAGNEDRIKLLLSGDNPNRINRELQYMGYVSQAQAKLIETLRANQQAIRANKAD 180 Query: 181 TQNAKFELEEIAQEELEQKKVLEREKGRRQTLLSQLSTKLNAQRQEAGRIEQDQQRLSSL 240 TQNAKFELEEIAQEELEQKKVLEREKGRRQTLLSQLSTKLNAQRQEAGRIEQDQQRLSSL Sbjct: 181 TQNAKFELEEIAQEELEQKKVLEREKGRRQTLLSQLSTKLNAQRQEAGRIEQDQQRLSSL 240 Query: 241 VDKLAQIIEQQKKAEAEAREKRRQEQLAKAKAEREKRLAEQRARAEKRAAEAAKAAKEGK 300 VDKLAQIIEQQKKAEAEAREKRRQEQLAKAKAEREKRLAEQRARAEKRAAEAAKAAKEGK Sbjct: 241 VDKLAQIIEQQKKAEAEAREKRRQEQLAKAKAEREKRLAEQRARAEKRAAEAAKAAKEGK 300 Query: 301 PAPKMPDPADAIDDDEPPQQASLGRNDTVPEPQPDNFGKPFASLRGQLHLPVKGDLMAKF 360 PAPKMPDPADAIDDDEPPQQASLGRNDTVPEPQPDNFGKPFASLRGQLHLPVKGDLMAKF Sbjct: 301 PAPKMPDPADAIDDDEPPQQASLGRNDTVPEPQPDNFGKPFASLRGQLHLPVKGDLMAKF 360 Query: 361 GSRRGDGPTSRGLFIRAHEGAEVRAVAAGRVVFADWLRGFGNLIIVDHGNQYMTIYGNNQ 420 GSRRGDGPTSRGLFIRAHEGAEVRAVAAGRVVFADWLRGFGNLIIVDHGNQYMTIYGNNQ Sbjct: 361 GSRRGDGPTSRGLFIRAHEGAEVRAVAAGRVVFADWLRGFGNLIIVDHGNQYMTIYGNNQ 420 Query: 421 SVLKRAGDLVKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFDPQGWVTSR 470 SVLKRAGDLVKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFDPQGWVTSR Sbjct: 421 SVLKRAGDLVKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFDPQGWVTSR 470 >gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillum seropedicae SmR1] Length = 254 Score = 72.8 bits (177), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 37/93 (39%), Positives = 58/93 (62%), Gaps = 2/93 (2%) Query: 371 RGLFIRAHEGAEVRAVAAGRVVFA-DWLRGFGNLIIVDHGNQYMTIYGNNQSVLKRAGDL 429 +G+ I EG V A AAG+V +A +RG+GN++I+ H Q +++Y +N+++L + G Sbjct: 155 KGIDIIGSEGQSVVAAAAGKVTYAGHGIRGYGNMVIIKHTPQLLSVYAHNKTILVKEGQT 214 Query: 430 VKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFD 462 V G IAT GNS N+ LYFE+R G+ + Sbjct: 215 VTRGQQIATMGNSDTNKVK-LYFEIRRNGKPIN 246 >gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 68.6 bits (166), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 39/121 (32%), Positives = 66/121 (54%), Gaps = 8/121 (6%) Query: 351 PVKGDLMAKFGSRRGDGPTSRGLFIRAHEGAEVRAVAAGRVVFA-DWLRGFGNLIIVDHG 409 P +G ++ F + +G+ I G V A AG+V++A +RG+GNL+I+ H Sbjct: 219 PAEGKVVGTFEGGK------KGVDIAGKAGQAVLAAGAGKVMYAGSGIRGYGNLVIIKHT 272 Query: 410 NQYMTIYGNNQSVLKRAGDLVKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFDPQGWVTS 469 + ++ Y +NQ++L + G V G IA G S ++ L+FE+R QG+ DP ++ Sbjct: 273 SNLLSAYAHNQTILVKEGQSVTKGQKIAEMGKS-DSDTVKLHFEIRQQGKPVDPSKYLPP 331 Query: 470 R 470 R Sbjct: 332 R 332 >gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 57.4 bits (137), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 25/68 (36%), Positives = 44/68 (64%) Query: 400 FGNLIIVDHGNQYMTIYGNNQSVLKRAGDLVKAGDTIATAGNSGGNEQSGLYFEMRYQGR 459 FGN++ +DHGN +T Y + + + GD+VK G +A G++G + + L+FE+R +G Sbjct: 238 FGNMLEIDHGNNIITRYAHASRLFVKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGI 297 Query: 460 AFDPQGWV 467 A DP+ ++ Sbjct: 298 AQDPRKFL 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.128 0.345 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 961,145 Number of extensions: 31758 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 4 Length of query: 470 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 381 Effective length of database: 1,198,455 Effective search space: 456611355 Effective search space used: 456611355 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)