BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0287.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC t... 595 e-171 gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC t... 595 e-171 gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC tr... 224 5e-60 gi|300312237|ref|YP_003776329.1| amino acid ABC transporter perm... 162 4e-41 gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC t... 151 8e-38 >gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 595 bits (1534), Expect = e-171, Method: Compositional matrix adjust. Identities = 309/309 (100%), Positives = 309/309 (100%) Query: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV Sbjct: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 Query: 61 APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM 120 APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM Sbjct: 61 APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM 120 Query: 121 MIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRAT 180 MIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRAT Sbjct: 121 MIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRAT 180 Query: 181 AENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV 240 AENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV Sbjct: 181 AENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV 240 Query: 241 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGI 300 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGI Sbjct: 241 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGI 300 Query: 301 MGERVADRA 309 MGERVADRA Sbjct: 301 MGERVADRA 309 >gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 595 bits (1534), Expect = e-171, Method: Compositional matrix adjust. Identities = 309/309 (100%), Positives = 309/309 (100%) Query: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV Sbjct: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 Query: 61 APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM 120 APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM Sbjct: 61 APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM 120 Query: 121 MIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRAT 180 MIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRAT Sbjct: 121 MIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRAT 180 Query: 181 AENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV 240 AENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV Sbjct: 181 AENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV 240 Query: 241 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGI 300 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGI Sbjct: 241 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGI 300 Query: 301 MGERVADRA 309 MGERVADRA Sbjct: 301 MGERVADRA 309 >gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 308 Score = 224 bits (572), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 137/314 (43%), Positives = 202/314 (64%), Gaps = 27/314 (8%) Query: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAPGLP 65 QQ++NGL LG++YALIA+GYTMVYG++ +INFAHG+I M+ + VGL L + V G P Sbjct: 10 QQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAIG-VQSGYP 68 Query: 66 GIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAMMIWGR 125 + L A++ ++ V + +ER+AYRPLR PRL PLI+AIG+SI LQ + G Sbjct: 69 LPLLLGGALIVSVLVTGLYGWTVERVAYRPLRGGPRLVPLISAIGMSIFLQNYVQIGQGA 128 Query: 126 SPLPFPQVMPSDPVHIAGAL-----------ISPTQIMLLALAVLAMVGLVLIVEKTKMG 174 + S PV I+GAL I ++++++ + ++ MV L L + +++MG Sbjct: 129 RDM-------SVPVLISGALEFQMGSDFTVTIPYSRMVIVGVTLVLMVALTLFIARSRMG 181 Query: 175 RAMRATAENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLK 234 RA RA AE+ +A L+G+D NKVI TF +GA LAA+ GV+ A +GF+ G+K Sbjct: 182 RACRACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFIAGIK 241 Query: 235 AFSAAVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLT 294 AF+AAVLGGIG+I GAMLGG+LLGL E+ AGY L S Y+D+ +F +L+++L Sbjct: 242 AFTAAVLGGIGSIPGAMLGGVLLGLAETFAAGY--------LPSEYKDVVSFGLLVLILL 293 Query: 295 LRPSGIMGERVADR 308 RP+G++G+ ++ Sbjct: 294 FRPTGLLGKPDVEK 307 >gi|300312237|ref|YP_003776329.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 162 bits (409), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 113/307 (36%), Positives = 186/307 (60%), Gaps = 20/307 (6%) Query: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAPGLP 65 QQ+IN L LGS+YAL ALG+T+V+GVL +IN +HG I M+G+ L L V+Q+A LP Sbjct: 4 QQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLL---VEQMA--LP 58 Query: 66 GIVQLVIAIVGAIPVCIVVSLLIERIAYRPLR--NAPRLAPLITAIGVSILLQTLAMMIW 123 V L++A+V ++ L+I+ + +PLR NAP L P+I IGV+I++ ++ ++ Sbjct: 59 LWVALLLAMVA----TGLLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGLF 114 Query: 124 GRSPLPFPQ-VMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRATAE 182 G FPQ +P + V + I+ Q+ ++ +A + MV L+ ++ KT++GRA+RA AE Sbjct: 115 GAENKRFPQGTIPEESVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAE 174 Query: 183 NPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAVLG 242 +P+ A L+G++ + ++T A L AGV+ +++ MG K + +LG Sbjct: 175 SPKAAYLLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILG 234 Query: 243 GIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGIMG 302 G+G+I GAM+GG+ LG E L YI S+++D AF +L ++L ++PSG+ G Sbjct: 235 GMGDIRGAMIGGLFLGFAEVLTVAYI--------SSDFRDAVAFGLLFLILLVKPSGMFG 286 Query: 303 ERVADRA 309 + + +A Sbjct: 287 KVLERKA 293 >gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 296 Score = 151 bits (381), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 99/313 (31%), Positives = 181/313 (57%), Gaps = 23/313 (7%) Query: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 MDI +Q + +G+ LG +YA+IA GY + + +NF G+ LM+GA+VGLS++ + Sbjct: 1 MDILLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNIH-- 58 Query: 61 APGLPGIVQLVIAIVGAIPVCIVVS----LLIERIAYRP-LRNAPRLAPLITAIGVSILL 115 G P + ++ IP+ +V + +E I RP ++ +++ I ++I+ Sbjct: 59 --GGPYLNYWLM-----IPIVLVFGALQGMFVEWIGVRPAIKIKSEFGWIMSTIALAIIF 111 Query: 116 QTLAMMIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGR 175 + +A IWG+ LPFP + P I GA + P Q++++ A+L M + L K+ G+ Sbjct: 112 KNVAENIWGKDDLPFPSPISGAPFQIFGANVQPMQVLVVVGALLIMAAVELFNRKSIYGK 171 Query: 176 AMRATAENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKA 235 A+ AT+ + AGLMG++ + VI ++A+ + AA AGV+ A + MG GLKA Sbjct: 172 AVVATSNDRDAAGLMGINTSMVITFSYALSSATAAFAGVL-VAPLTLTGATMGAALGLKA 230 Query: 236 FSAAVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTL 295 F+ A++GG+ + GA++GG++LG+ E+ LTG ++ + Y+++ ++L++VL + Sbjct: 231 FAVAIIGGLTSGMGAIVGGLILGVAET--------LTGFYISTGYKEVPGLVLLLLVLAV 282 Query: 296 RPSGIMGERVADR 308 +P+G+ G+ + Sbjct: 283 KPAGLFGKTAIKK 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.144 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 808,401 Number of extensions: 29221 Number of successful extensions: 265 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 229 Number of HSP's successfully gapped: 33 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)